Difference between revisions of "Team:Lambert GA/Experiments"

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<div id="subheading1"><b>Work Flow</b><div>
 
<div id="subheading1"><b>Work Flow</b><div>
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<b><a style="color:black;" href="#Step1">Miniprep/Nanodrop</a></b>
 
<b><a style="color:black;" href="#Step1">Miniprep/Nanodrop</a></b>
 
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<b><a style="color:black;" href="#Step2">Digest</a></b>
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<div id="subheading1"><b>Materials</b><div>
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Miniprep: grown culture, microcentrifuge, 2 1.5mL microcentrifuge tubes, mini column and collection tube, Solution I, Solution II, Solution III, HBC Wash Buffer, DNA Wash Buffer, Elution Buffer, micropipette and tips
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Nanodrop: nanodrop machine, miniprepped DNA, Kimtech wipes, micropipette and tips
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Digest: miniprepped DNA, dH₂O, 10X RE-Mix, standard restriction enzyme, micropipettes and tips
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Gel: agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
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Ligation: vector, parts 1 and 2, ligase buffer, ligase, Antarctic phosphatase, microcentrifuge tube, ice, micropipette and tips
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Transformation: ice, ligation mixture, competent cells, incubator, LB media, microcentrifuge tubes, micropipette and tips Plate: agar plate, micropipette and tips, beads
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Colony PCR: dH₂O, buffer, VF₂, VR, Q5 polymerase, dNTP, DNA dilution, micropipette and tips, PCR tubes, thermocycler, ice
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Gel: agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
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Inoculate: LB media, dilution, micropipette and tips
  
 
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Revision as of 00:42, 15 October 2018

CALM ELECTROPEN
BACKGROUND DATA & METHODS RESULTS DISCUSSION COLOR Q SUPPLEMENTARY REFERENCES
COLOR Q CHROME Q REFERENCES
PRINCIPLES & DESIGN MODEL & THEORY PRODUCT DESIGN REFERENCES
INTEGRATED HUMAN PRACTICES EDUCATION & ENGAGEMENT COLLABORATIONS
BASIC PARTS COMPOSITE PARTS
DESCRIPTION BACKGROUND DESIGN EXPERIMENTS LAB NOTEBOOK INTERLAB RESULTS DEMONSTRATE THE VISION
TEAM MEMBERS ATTRIBUTIONS
E X P E R I M E N T S



































Wet Lab Summary


Gaurav add wet lab summary


Work Flow


Click on the step to be directed to the procedure!
Miniprep/Nanodrop
Digest


Materials


Miniprep: grown culture, microcentrifuge, 2 1.5mL microcentrifuge tubes, mini column and collection tube, Solution I, Solution II, Solution III, HBC Wash Buffer, DNA Wash Buffer, Elution Buffer, micropipette and tips
Nanodrop: nanodrop machine, miniprepped DNA, Kimtech wipes, micropipette and tips
Digest: miniprepped DNA, dH₂O, 10X RE-Mix, standard restriction enzyme, micropipettes and tips
Gel: agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
Ligation: vector, parts 1 and 2, ligase buffer, ligase, Antarctic phosphatase, microcentrifuge tube, ice, micropipette and tips
Transformation: ice, ligation mixture, competent cells, incubator, LB media, microcentrifuge tubes, micropipette and tips Plate: agar plate, micropipette and tips, beads
Colony PCR: dH₂O, buffer, VF₂, VR, Q5 polymerase, dNTP, DNA dilution, micropipette and tips, PCR tubes, thermocycler, ice
Gel: agarose gel (make one if necessary), 1X TAE Buffer, power supply, chamber and electrodes, ladder, micropipette and tips, DNA
Inoculate: LB media, dilution, micropipette and tips