Difference between revisions of "Team:Aalto-Helsinki"

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<div class="title_style animate_title" id="title1"> <br>Our Mission</div>
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<div id="title2" class="title_style animate_title"> <br>Our Project</div>
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<div id="title2" class="title_style animate_title">SILKOLOR</div>
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<div class="text_style">A sustainable approach to dyeing industry using fusion proteins</div>
 
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<div class="title_style animate_title" id="title3"><br> Our Lab</div>
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<div class="title_style animate_title" id="title3">Problem <br><br>2nd<br>
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textile dyeing is the second largest cause of water pollution
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<div class="text_style"><br><br> <p>The parts of our fusion proteins were ordered as separate sequences: mRPF, Prancer Purple, sgBP, silk repeats, cellulose binding module, and keratin binding domain. Constructs containing a chromoprotein sequence, a binding domain, and silk repeats in different combinations were assembled with Golden Gate. Purified and evaluated plasmids were transformed in <i>E. coli</i> production strain BL21, and the constructs were produced by inducing with IPTG. Proteins were purified with ÄKTA or a heat-based protocol based on our modeling results.</p>
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<p>The tests performed included:</p>
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<li>The effect of protein production on cell growth.</li>
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<li>The binding of the cellulose binding module to nanocellulose and untreated cotton.</li>
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<li>The binding of the keratin binding domain to a keratin supplement and hair.</li>
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<li>The production of colorful silk fibers.</li>
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<div class="title_style animate_title" id="title4"><br> Our Team </div>
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<div class="title_style animate_title" id="title4">Solutions</div>
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  <li>Pre-dyed silk fibers</li>
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  <li>Chromoprotein based dye with cellulose binding domain</li>
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  <li>Chromoprotein based dye with keratin binding domain</li>
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<div class="title_style animate_title" id="title5"> <br>Our Story <br><br> </div>
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Revision as of 09:06, 15 October 2018

Our Mission
SILKOLOR
A sustainable approach to dyeing industry using fusion proteins
Problem

2nd
textile dyeing is the second largest cause of water pollution
Solutions
  • Pre-dyed silk fibers
  • Chromoprotein based dye with cellulose binding domain
  • Chromoprotein based dye with keratin binding domain
Lab