Difference between revisions of "Team:Munich/chibiobrick1.html"
Franziwinzig (Talk | contribs) |
Franziwinzig (Talk | contribs) |
||
| Line 63: | Line 63: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/7/7e/T--Munich--WL1_Restriction_Digest.pdf" target="_blank"> | + | <a href="https://static.igem.org/mediawiki/2018/7/7e/T--Munich--WL1_Restriction_Digest.pdf" target="_blank">Restriction digest</a>, |
<a href="https://international.neb.com/products/t1030-monarch-pcr-dna-cleanup-kit-5-ug#Product%20Information" target="_blank">PCR purification</a>, | <a href="https://international.neb.com/products/t1030-monarch-pcr-dna-cleanup-kit-5-ug#Product%20Information" target="_blank">PCR purification</a>, | ||
<a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, | <a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, | ||
Revision as of 14:07, 15 October 2018
Forming oligodimers from single-strand DNA chi6 sequences
2018/06/01| Participants: | Dominic Schwarz |
| Protocol: | Oligodimerization |
Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/04| Participants: | Dominic Schwarz |
| Protocol: | Restrition digest, Ligation, Chemical transformation, Mini prep, Sequencing |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment. |
Redo: Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/06 - 2018/06/26| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, PCR purification, Ligation, Chemical Transformation, PCR, Agarose gel |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | No bands on the Gel. Colonies on the plate resulted from contamination. |