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Revision as of 15:33, 15 October 2018

Template loop detected: Template:Grenoble-Alpes

ATTRIBUTIONS

Through this page, we want to explain the role that each team member has had within the project. We want also to make known, and thank, the other actors of the project who participated in its smooth running.
But before that we will present rapidly our iGEM Grenoble history!

iGEM GRENOBLE-ALPES STORY

1) How did we know iGEM ?

We have never had lessons on iGEM but each member of the team has heard about the competition at Grenoble Alpes University (UGA) or INP school, by the members of the previous team and the teachers who took them framed.
Then, the team met last November via Pierre Cavailles (the main advisor and a biotechnology teacher at the UGA), and that's how the story started.

2) Project Chronology

You can find, in the diagram below, the chronology of the project which summarizes the key points of our work.

NEED DIAGRAM

Attribution

This part describes the work done, and the person who performed it.
The legend below could help you to know if it is a team member, an advisor, an instructor or an outsider thanks to the color code :

Team members
Advisors
Instructors
Outsiders

Engineering

WHAT THE TEAM DID

DNA extraction module & Phages-bacteria Modeling
Thomas Goyet

Temperature control system & Transformation Module
Xandre Gallard

Fluorescence Sensor module
Charles Jabour

Pipetting Module
Jean Le Pennec

Overall 3D Model of the system
Mainly Lucas Sancéré with the help of Jean Le Pennec & Thomas Goyet

Overall code for the control of the system
Jean Le Pennec & Thomas Goyet

PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:

Lab and premise support

Ugo Hevin, Franz Bruckert & Marianne Weidenhaupt of the CIME Nanotech Laboratory (Minatec, Grenoble) Nicolas Ruty & Julien Traveaux of the Phelma-INP school

Difficult technique support

Engineers of BioMérieux : Hervé Rostaing (general engineering support), Blaze Jérome (mechanics and machining of complex parts of the system), Frédéric Pinston (fluorescence detection) & Alain Laurent (magnetic beads).

Biology

WHAT THE TEAM DID

Experiments on bacteriophages & their capacity to lyse bacteria
Cécile Razimbaud & Yasmine Taveau

DNA extraction with magnetic beads
Cécile Razimbaud

Construction of the DNA probe :
- detecting an antibiotic resistance marker of Pseudomonas a. PA01
Elise Aubert
- detecting a sequence of an housekeeping gene of Pseudomonas a. PA01
Fanny Garon

Conservation of biological products & Freeze-drying
Perrine Rochas

BBa_J04450 characterisation
Perrine Rochas with the help of Xandre Gallard

PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:

Lab support

Sophie Boretti, Martin Ghis and “practical work” service members in general (first floor of Jean Roger Laboratory (University of Grenoble-Alpes)).
The fifth floor members of Jean Roger Laboratory including Pierre Cavailles.
Hélène Coradin, Béatrice Schaack (for her freeze-drying help), and the other members of the eighth floor of Jean Roger Laboratory.


Biology support

Pierre Cavailles & Clément Caffaratti who provided a lot of advice about the Biology and an outstanding support throughout the project.


Bacteriophages experiments support - General support & donation of T5 bacteriophages and their host E. coli F

Cécile Breyton of the Grenoble IBS (Institute of Structural Biology)


Donation of Pseudomonas a. PA01

Ina Attrée of the Grenoble CEA (Office of the Atomic Energy and Alternative Energies)


Donation and sending of PAK-P3 bacteriophages and their host Pseudomonas a. PAK

Laurent Debarbieux of the Pasteur Institute of Paris


Donation of Staphylococcus aureus strains and a specific bacteriophage Sb-1

Grégory Resch & Jonathan Save of the University of Lausanne (Swiss)


Feedback and advices about detection experiments on bacteriophages

Pierre Marcoux of the Grenoble CEA (Office of the Atomic Energy and Alternative Energies)

FURTHER INFORMATIONS AND ACKNOWLEDGEMENTS

Pseudomonas aeruginosa PAO1 and their specific phages HER18 the team needed to find a class 2 laboratory which accepts to welcome us. Javier Yugueros Marcos, the main BioMérieux contact, have immediately responded to this call for help. With the assistance of Patrice Gracieux, he organized the coming of Cécile Razimbaud in BioMérieux lab (about security and others). Stéphanie Borland has accompanied Cécile during all the experiments, mostly for the safety rules.