Difference between revisions of "Team:Bio Without Borders/Design"

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<h1>Design</h1>
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<section class="spotlight">
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<p>
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<div class="image"><img src="https://static.igem.org/mediawiki/2018/5/55/T--Bio_Without_Borders--HorseshoeCrabs.png" alt="" /></div><div class="content">
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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<h2>Creating Factor C<br /></h2>
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</p>
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<p>We used an NEBuilder kit to combine two sequence that IDT sent us.</p>
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<div class="image"><img src="https://static.igem.org/mediawiki/parts/6/6b/T--Bio_Without_Borders--Outreach_1.png" alt="" /></div><div class="content">
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<h2>Creating a linker protein of the cellulose-binding domain (CBD) and GFP
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</h2>
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<p> This functions as the reporter protein which would demonstrate Factor C's ability to cleave.</p>
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<section class="spotlight">
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<div class="image"><img src="https://static.igem.org/mediawiki/2018/7/72/T--Bio_Without_Borders--Team.png" alt="" /></div><div class="content">
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<h2>Expressing Factor C through Pichia pastoris</h2>
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<p> After making our parts, we wanted to express the parts in an easy model organism like pichia. </p>
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2018.igem.org/Team:Bio_Without_Borders/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
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<h3>What should this page contain?</h3>
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<ul>
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<li>Explanation of the engineering principles your team used in your design</li>
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<li>Discussion of the design iterations your team went through</li>
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<li>Experimental plan to test your designs</li>
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<h3>Inspiration</h3>
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
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Revision as of 00:08, 18 October 2018

Creating Factor C

We used an NEBuilder kit to combine two sequence that IDT sent us.

Creating a linker protein of the cellulose-binding domain (CBD) and GFP

This functions as the reporter protein which would demonstrate Factor C's ability to cleave.

Expressing Factor C through Pichia pastoris

After making our parts, we wanted to express the parts in an easy model organism like pichia.