Difference between revisions of "Team:Munich/preparingge.html"

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       <td>Protocol:</td>
 
       <td>Protocol:</td>
 
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<a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">ELectrocompetent transformation</a>
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<a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Electrocompetent transformation</a>
 
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Revision as of 12:19, 16 October 2018

Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering

2018/08/28
Participants: Dominic Schwarz
Protocol: Electrocompetent transformation
Notes: inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.
Results: ~200 colonies