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All three subunit of Glycolate Oxidase(GO) are shown in the SDS-PAGE. GO contains three subunits, D,E, and F, which weigh 55.5 kDa, 38.3 kDa, and 45.0 kDa respectively. Lane 1 was BL21(DE3) with pETDuet-GO, but without IPTG induction process. Lanes 2 and 3 were BL21(DE3) with empty pETDute vectors and contain 0.1 and 0.2 mM IPTG. Lanes 4 to 7 are BL21(De3) with pETDuet-GO. Lanes 4 and 6 were inducted with 0.1mM IPTG, and lanes 5 and 7 were inducted with 0.2mM IPTG. All the inductions were done at 30ºC. | All three subunit of Glycolate Oxidase(GO) are shown in the SDS-PAGE. GO contains three subunits, D,E, and F, which weigh 55.5 kDa, 38.3 kDa, and 45.0 kDa respectively. Lane 1 was BL21(DE3) with pETDuet-GO, but without IPTG induction process. Lanes 2 and 3 were BL21(DE3) with empty pETDute vectors and contain 0.1 and 0.2 mM IPTG. Lanes 4 to 7 are BL21(De3) with pETDuet-GO. Lanes 4 and 6 were inducted with 0.1mM IPTG, and lanes 5 and 7 were inducted with 0.2mM IPTG. All the inductions were done at 30ºC. | ||
</p> | </p> | ||
− | <img src = "https://static.igem.org/mediawiki/2018/a/a6/T--RHIT--SDSpage.jpg"></li> | + | <center> |
+ | <img style="width:50%" src = "https://static.igem.org/mediawiki/2018/a/a6/T--RHIT--SDSpage.jpg"></center></li> | ||
<li> <p style="font-weight:bold"> BBa_K2716003 - Terminator used in both plasmids </p> Terminator for E. coli pSB1C3 backbone used in BBa_K2716100 and BBa_K2716102 composite parts. <br><br> </li> | <li> <p style="font-weight:bold"> BBa_K2716003 - Terminator used in both plasmids </p> Terminator for E. coli pSB1C3 backbone used in BBa_K2716100 and BBa_K2716102 composite parts. <br><br> </li> | ||
</ul> | </ul> |
Revision as of 23:08, 15 October 2018