Peristaltic Pump (IMPROVEMENT)

Function

The peristaltic pump displaces fluid, such that bacterial culture is kept flowing through the cuvette. This allows the sensor to take continuous measurements.

Design

This peristaltic pump is an improvement on the design by the 2015 Aachen iGEM team. We chose to build on their design since their pump was also designed for continuous pumping in a bioreactor. Three modifications were made.

Improvement 1^[a]

The first modification replaces their pentagonal spring rotor base with a circular rotor base (Figure 1).

This was so that we could countersink flat-head bolts into the rotor structure, compared to having raised bolts such as pan-head bolts (Figure 2).

We thickened the wall separating the fastening nut and the shaft by 2.75 mm, placing the nut 6mm away from the centre of the shaft (Figure 4). We also thickened the wall between the bolt head and the nut. This design can thus better withstand mechanical stresses, and is more durable.

Improvement 3

The third modification we made was to reposition the inlet and outlet for the silicone tubing.

Aachen 2015’s design stacked several Plexiglass layers on the stepper motor’s faceplate, forming a housing to hold a silicone tube inside a circular path. We noticed that Aachen 2015’s silicone tubes had a smaller outer and inner diameter than the tubes we planned to use, as the working volume of their bioreactor is less than ours. After analyzing their design, we conjectured that we only needed to modify the thickness of the pumping layer (Figure 5) to accommodate our own silicone tubing.

However, our silicone tube exerted forces on the walls of the pumping layer and caused the walls to deflect (Figure 6).

Because of this, the silicone tube could not be pinched closed (occluded), and the pump was unable to force the fluid to move through the tube.

We thus repositioned the inlet and outlet, combining them into one opening (Figure 7).

Now, when the silicone tube attempts to regain its original, non-deformed shape, the lines of action of the forces it exerts at the opening will be much closer to the metal fasteners (Figure 8), causing a much smaller bending moment. Additionally, the force exerted by the silicone tube is now shared by 4 fasteners instead of 2 as the shape is now continuous.

After this modification, the pumping layer no longer deflected visibly, and operation was smooth.

Testing

We validated the functionality of this component and characterized it by plotting the mass flow rate as a function of RPM.

Procedure

1. Place a length of silicone tubing in the peristaltic pump. Put one end into a water reservoir. Place another end into a clean, empty beaker. Put the empty beaker on an electronic balance.
2. Use the code to set the RPM.
3. Turn the peristaltic pump on.
4. Wait approximately 5 seconds for the flow to stabilize. When the flow has stabilized, start the stopwatch while simultaneously taring the electronic balance.
5. Read the mass off the display on the electronic balance every 10 seconds, for 5 minutes total.
6. After completing Step 4, stop the stopwatch and empty the beaker. Refill the water reservoir if necessary.
7. Repeat Steps 3-5 twice more.
8. Represent the measurements with a scatter plot. Find the trendline. The gradient is the mass flow rate [g/s]. While kg is the correct SI unit, we find g to be more helpful here.
9. Record mass flow rate and RPM.
10. Repeat steps 4-9 for different RPMs.
11. Plot a graph of flow rate against RPM. You may now use this graph to find out what RPM you should enter in the code for your desired mass flow rate.



Results





Construction




Ever wanted a peristaltic pump of your own? Now you can have one! Just follow these steps!




Bill of Materials




All fasteners are M3 size.









Structural Assembly







STL and dxf


Electronics





Code