Difference between revisions of "Team:NDC-HighRiverAB/Parts"

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<p>This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (BBa_R0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:</p>
 
<p>This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (BBa_R0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:</p>
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<p> - First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in pseudomonas aeruginosa. (Part:BBa_K2694001) </>
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  <li>First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in Pseudomonas aeruginosa. (Part:BBa_K2694001) </li>
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<p> - For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli. (Part:BBa_R0011) </p>
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  <li>For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli. (Part:BBa_R0011) </li>
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<p> - The RBS we used is B0034. (Part:BBa_B0034) </p>
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  <li>The RBS we used is B0034. (Part: BBa_B0034) </li>
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<p> - Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part:BBa_B0015) </p>
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  <li>Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part: BBa_B0015) </li>
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<p>- After we had chosen all of our biobrick parts, we combined them into the plasmid BBa_K2694000.
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  <li>After we had chosen all of our biobrick parts, we combined them into the plasmid BBa_K2694000.</li>
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</p>
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<div> <a href="http://parts.igem.org/Part:BBa_K2694000">BBa_K2694000</a> </div>
 
<div> <a href="http://parts.igem.org/Part:BBa_K2694000">BBa_K2694000</a> </div>

Revision as of 02:17, 17 October 2018

Parts

This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (BBa_R0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:

  • First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in Pseudomonas aeruginosa. (Part:BBa_K2694001)
  • For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli. (Part:BBa_R0011)
  • The RBS we used is B0034. (Part: BBa_B0034)
  • Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part: BBa_B0015)
  • After we had chosen all of our biobrick parts, we combined them into the plasmid BBa_K2694000.