Difference between revisions of "Team:Munich/qpcr1.html"

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Forming oligodimers from single-strand DNA chi6 sequences
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Gel extraction of TP01 and T7p29 PCR product
 
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       <td>Participants:</td>
 
       <td>Participants:</td>
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       <td>Dominic Schwarz</td>
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       <td>Nils O'Brien</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
 
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       <td>
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<a href="https://www.sigmaaldrich.com/technical-documents/protocols/biology/annealing-oligos.html" target="_blank">Oligodimerization
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<a href="HERE" target="_blank">NAME</a>,
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</a>
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<a href="HERE" target="_blank">NAME</a>
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PCR from May 28th, NEB Gel extraction Kit (Monarch #T10265)
  
 
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Revision as of 08:35, 16 October 2018

Forming oligodimers from single-strand DNA chi6 sequences

2018/05/28
Participants: Nils O'Brien
Protocol: Gel extraction, NEB Q5 High-Fidelity DNA Polymerase Kit #M0492S, NEB Taq Polymerase Kit (nummer)
Notes: T7 Phage:
TP01_gene (qPCR_Fwd_T7p01, qPCR_Rvd_T7p01)
TP07_gene (qPCR_Fwd_T7p07, qPCR_Rvd_T7p07) --> did not work
T7p29_gene (qPCR_Fwd_T7p29, qPCR_Rvd_T7p29)
RzLys_gene (qPCR_RzLys_F, qPCR_RzLys_R)
E.coli K12 for T7:
CysG_gene (qPCR_cysG_F, qPCR_cysG_R)
idnT_gene (qPCR_cidnT_F, qPCR_cidnT_R)
RrsA_gene (qPCR_rrsA_F, qPCR_rrsA_R) RFP
mRFP_gene (fwd_RFP_qPCR,rvd_RFP_qPCR)
Results: Important: TP07 Primer does not work; T7p29 and TP01 primers work

Gel extraction of TP01 and T7p29 PCR product

2018/06/01
Participants: Nils O'Brien
Protocol: NAME, NAME PCR from May 28th, NEB Gel extraction Kit (Monarch #T10265)
Notes: here
Results: here