Difference between revisions of "Team:Bio Without Borders/Results"

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<h3>What should this page contain?</h3>
 
<h3>What should this page contain?</h3>
 
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> We succeeded in cloning the two proteins that make up the test system: Factor C and our substrate (GFP-linker-CDB-CBD)  </li>
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<li> Future plans for the project. </li>
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<li> We submitted them to the iGEM registry in pSB1C3. </li>
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<li> Considerations for replicating the experiments. </li>
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<li> We attempted to express Factor C in Pichia pastoris, a yeast expression system. </li>
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<li> We attempted to express the substrate in E. coli. </li>
 
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<div class="column two_thirds_size" >
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<h3>Describe what your results mean </h3>
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<h3>Cloning and expression of Factor C </h3>
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<ul>
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<p> Our first choice was whether to express the Factor C gene from the Atlantic horseshoe crab (Limulus polyphemus) or from the Japanese Horseshoe crab (Tachypleus tridentatus). The latter had been described more fully in the literature. But we wanted to use the one from OUR homeland. We codon-optimized it for our preferred expression system, Pichia pastoris, and sent it for synthesis. Unfortunately, after several failed attempts, IDT was unable to synthesize it. So we switched to the Japanese horseshoe crab and had it synthesized as G-blocks for later assembly, and also as a complete gene. Our attempts at G-block assembly failed, and we ended up using the full gene synthesized in the plasmid pIDT:
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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</p>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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Revision as of 03:02, 18 October 2018

Results

Here you can describe the results of your project and your future plans.

What should this page contain?

  • We succeeded in cloning the two proteins that make up the test system: Factor C and our substrate (GFP-linker-CDB-CBD)
  • We submitted them to the iGEM registry in pSB1C3.
  • We attempted to express Factor C in Pichia pastoris, a yeast expression system.
  • We attempted to express the substrate in E. coli.

Cloning and expression of Factor C

Our first choice was whether to express the Factor C gene from the Atlantic horseshoe crab (Limulus polyphemus) or from the Japanese Horseshoe crab (Tachypleus tridentatus). The latter had been described more fully in the literature. But we wanted to use the one from OUR homeland. We codon-optimized it for our preferred expression system, Pichia pastoris, and sent it for synthesis. Unfortunately, after several failed attempts, IDT was unable to synthesize it. So we switched to the Japanese horseshoe crab and had it synthesized as G-blocks for later assembly, and also as a complete gene. Our attempts at G-block assembly failed, and we ended up using the full gene synthesized in the plasmid pIDT:

Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

  • A list of linked bullet points of the successful results during your project
  • A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

Inspiration

See how other teams presented their results.