Difference between revisions of "Team:HZAU-China/InterLab"

To obtain the OD600 reference point of a particular plate reader is rather necessary since we can only obtain ABS from a plate reader which is different from OD gained from a spectrophotometer. The ABS data obtained from a plate reader is volume-dependent while the sample of a spectrophotometer are volume-fixed.

As the protocol instructed, we added 100μL LUDOX solution into wells A1, B1, C1, D1 while added 100μL ddH₂O into wells A2, B2, C2, D2. Then, we measured OD600 of the samples in the plate reader (Table 1).

Table 1. The result of OD600 reference point measuring.

The data showed that the OD600 reference point of our plate reader is 3.500.

The particle standard curve experiment aims to obtain the relationship curve between the ABS600 of the solution and the concentration of bacteria. To achieve this goal, we need to find a kind of material which resembles bacteria very much. The headquarters sent us with Silica Beads, a kind of little particle which met our needs.

We made up a series of solution of different but continuous magnitude. To make the experiment more precise, we also set up another three parallel groups(Figure 1).

The result of Particle Standard Curve

The fluorescence standard curve experiment targets to obtain the relationship curve between the strength of fluorescence and the concentration of fluorescein which has similar excitation and emission properties to GFP.

We prepared a series of fluorescein solution of different but continuous magnitude. As every experiment required, we also set up 4 parallel groups. Then we measured the strength of the fluorescence of each sample(Figure 2).