Line 163: | Line 163: | ||
<div class="chapai"><br /><br /> | <div class="chapai"><br /><br /> | ||
− | <h4 ><font size=" | + | <h4 ><font size="6">Elementary Operation</font></h4 > |
<br />Conservation | <br />Conservation | ||
<br />Gel Electrophoresis | <br />Gel Electrophoresis | ||
Line 175: | Line 175: | ||
<br />Sterilization | <br />Sterilization | ||
<br />Transformation of Escherichia coli | <br />Transformation of Escherichia coli | ||
− | <h4 ><font size=" | + | <h4 ><font size="6">Systems Detection</font></h4 > |
<br />Assay of Gene Expression by Flow Cytometry | <br />Assay of Gene Expression by Flow Cytometry | ||
<br />Fluorescence measurement | <br />Fluorescence measurement | ||
Line 196: | Line 196: | ||
</div> | </div> | ||
− | + | <br /><br /><br /> | |
Revision as of 16:20, 16 October 2018
Experiments
This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.
Elementary Operation
Conservation
Gel Electrophoresis
Gel Extraction
Inoculation
Ligation system
medium
Plasmid extraction
Polymerase Chain Reaction
Restriction Enzyme Digestion
Sterilization
Transformation of Escherichia coli
Systems Detection
Assay of Gene Expression by Flow Cytometry
Fluorescence measurement
miniToe Polycistron Fluorescence measurement
The application of miniToe——Regulation of motA
Contact Us : oucigem@163.com | ©2018 OUC IGEM.All Rights Reserved. | …………