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<h3></h3> | <h3></h3> | ||
This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail. | This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail. | ||
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− | <div class="chapai" | + | <div class="chapai"><br /> |
<h4 ><font size="6">Elementary Operation</font></h4 > | <h4 ><font size="6">Elementary Operation</font></h4 > | ||
<br />Conservation | <br />Conservation | ||
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<br />Restriction Enzyme Digestion | <br />Restriction Enzyme Digestion | ||
<br />Sterilization | <br />Sterilization | ||
− | <br />Transformation of Escherichia coli | + | <br />Transformation of Escherichia coli <br /><br /> |
<h4 ><font size="6">Systems Detection</font></h4 > | <h4 ><font size="6">Systems Detection</font></h4 > | ||
<br />Assay of Gene Expression by Flow Cytometry | <br />Assay of Gene Expression by Flow Cytometry | ||
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<br />miniToe Polycistron Fluorescence measurement | <br />miniToe Polycistron Fluorescence measurement | ||
<br />The application of miniToe——Regulation of motA | <br />The application of miniToe——Regulation of motA | ||
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Revision as of 16:21, 16 October 2018
Experiments
This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.
Elementary Operation
Conservation
Gel Electrophoresis
Gel Extraction
Inoculation
Ligation system
medium
Plasmid extraction
Polymerase Chain Reaction
Restriction Enzyme Digestion
Sterilization
Transformation of Escherichia coli
Systems Detection
Assay of Gene Expression by Flow Cytometry
Fluorescence measurement
miniToe Polycistron Fluorescence measurement
The application of miniToe——Regulation of motA
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