Difference between revisions of "Team:Edinburgh OG/Parts"

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      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter </td>
 
      <td>The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter </td>
 
      <td>1646bp</td>
 
      <td>1646bp</td>
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      <td>Original Sequence obtained from: NCBI(AM260479.1) and BBa_K390501. Codon Optimised for E.coli</td>
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      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a> Codon Optimised for E.coli</td>NCBI(AM260479.1) and BBa_K390501. Codon Optimised for E.coli</td>
 
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      <td>Co-expression of Bktb with PHA operon</td>
 
      <td>Co-expression of Bktb with PHA operon</td>
 
      <td>5462bp</td>
 
      <td>5462bp</td>
−
      <td>The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCAB is BBa_K1149051</td>
+
      <td>Original Sequence obtained from: <a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_015726.1?report=genbank&from=1485802&to=1486986">NCBI (NC_015726.1)</a>. Codon Optimised for E.coli. The part of Hybrid promoter-PhaCAB is BBa_K1149051</td>
 
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Revision as of 18:01, 16 October 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

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Parts

NCBI(AM260479.1). Codon Optimised for E.coliNCBI(AM260479.1) and BBa_K390501. Codon Optimised for E.coli
Registry Number Brief Description Key Details Length/th> Sequence information
BBa_K2739005 proR-PhaR-Pha promoter-PhaP The phasin autoregulation system with its native promoter + phasin with its native promoter 1459bp Original Sequence obtained from: NCBI(AM260479.1) Codon Optimised for E.coli
BBa_K2739006 proR-PhaR-proP-PhaP-HlyA The phasin autoregulation system with its native promoter + phasin-HlyA signal peptide with its native promoter 1646bp Original Sequence obtained from: NCBI(AM260479.1) Codon Optimised for E.coli
BBa_K2739007 LacP-RBS-PhaP-HlyA-Terminator Phasin with HlyA signal peptide under the control of Lac promoter 1139bp This part is developed from BBa_K390501
BBa_K2739009 Hybrid promoter-PhaCAB-Bktb Co-expression of Bktb with PHA operon 5462bp Original Sequence obtained from: NCBI (NC_015726.1). Codon Optimised for E.coli. The part of Hybrid promoter-PhaCAB is BBa_K1149051
BBa_K2739010 Hybrid promoter-PhaCB-Bktb Bktb in replacing the phaA in PHA operon 4252bp The sequence of Bktb is obtained from NCBI and being codon optimised to E.coli. The part of Hybrid promoter-PhaCB is developed from BBa_K1149051