Difference between revisions of "Team:Vilnius-Lithuania/Attributions"

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<strong>Audrius Žemys</strong>
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<strong>Justinas Iljeitis
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<p>For organizing and hosting Lithuania’s first BioHackathon together with us.</p>
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</strong>
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<p>Who helped us with public relations and marketing at the beginning of the project.
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</p>
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<strong>Justina Žvirblytė</strong>
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<p>Who was involved in developing our idea as well as helped a lot with the InterLab study measurements.
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</p>
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<strong>Lina Bivainienė
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</strong>
 +
<p>Who supported us while planning the DNA day celebration.
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</p>
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<strong>Prof. Aurelija Žvirblienė
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</strong>
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<p>Who provided a theoretical background of antibody-antigen interactions.
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</p>
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<strong>Milda Zilnytė
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</strong>
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<p>Who helped us plan and perform scFv activity detection experiments.</p>
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<strong>Dr. Violeta Jonušienė
 +
</strong>
 +
<p>Who provided us with lab gear for SDS-PAGE.
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</p>
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<strong>Dovilė Strepetkaitė
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</strong>
 +
<p>Who provided us with a Precast Gel system and explained how to use it.</p>
 +
<strong>Prof. Christoph Flamm</strong>
 +
<p>Who surprised us with his willingness to teach us how to design custom RNA structures in silico and helped us create synthetic RNA thermometers.
 +
</p>
 +
<strong>Prof. Ivo Hofacker
 +
</strong>
 +
<p>Another renown specialist in RNA Bioinformatics, who dedicated his time and consulted us purely out of kind will on the design and applications of RNA thermoswitches.
 +
</p>
 +
 
 +
<strong>Tadas Rimkus-Masaitis
 +
</strong>
 +
<p>Who kindly gave us a lot of valuable advice for problem-solving as an IT specialist.
 +
</p>
 +
<strong>Povilas Sidaravičius</strong>
 +
<p>Who enthusiastically mentored all the teams during the BioHackathon and inspired us to develop the idea of LipoVision.
 +
 
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Revision as of 19:40, 16 October 2018

Attributions

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Cell-free systems are becoming an increasingly popular in vitro tool to study biological processes as it is accompanied by less intrinsic and extrinsic noise. Relying on fundamental concepts of synthetic biology, we apply a bottom-up forward engineering approach to create a novel cell-free system for unorthodox protein-evolution. The core of this system is cell-sized liposomes that serve as excellent artificial membrane models. By encapsulating genetic material and full in vitro protein transcription and translation systems within the liposomes, we create reliable and incredibly efficient nanofactories for the production of target proteins. Even though there are many alternative proteins that can be synthesized, our main focus is directed towards membrane proteins, which occupy approximately one third of living-cells’ genomes. Considering their significance, membrane proteins are spectacularly understudied since synthesis and thus characterization of them remain prevailing obstacles to this day. We aim to utilize liposomes as nanofactories for directed evolution of membrane proteins. Furthermore, by means of directed membrane protein-evolution, a universal exposition system will be designed in order to display any protein of interest on the surface of the liposome. This way, a system is built where a phenotype of a particular protein is expressed on the outside while containing its genotype within the liposome. To prove the concept, small antibody fragments will be displayed to create a single-chain variable fragment (scFv) library for rapid screening of any designated target.

Special Thanks

Dr. Linas Mažutis

Sector of Microtechnologies

Even though Dr. Mažutis was mostly working abroad, he never hesitated to find extra time for an internet call and give some fundamental advice in the field of microfluidics. The opportunity to work in the microfluidics’ laboratory was also invaluable for building our project.

Dr. Gintaras Valinčius

Department of Bioelectrochemistry and Biospectroscopy

Dr. Gintaras Valinčius was always optimistic about our team and was always curious about the current state of our project. Despite Dr. Valinčius busy schedule, he was always willing to help in any way he could. His expertise in membrane science was particularly helpful.

Professor Edita Sužiedėlienė

Department of Biochemistry and Molecular Biology

Prof. Edita Sužiedėlienė’s support has been invaluable to Lithuanian iGEM teams since the establishment of the first team back in 2015. She has been kind enough to let us use her lab regularly and was patient about missing equipment after intensive work routines.

Tadas Penkauskas

Department of Bioelectrochemistry and Biospectroscopy

Tadas Penkauskas’ experience in membrane-based science and willingness to help with any matter related to lipids and membranes always helped us move further. Additionally, Tadas’ bulk-synthesized liposomes were a powerful tool to test our membrane proteins in higher throughput, while our method was still being developed.

Aistė Skrebytė

Head of Rector’s Office at Vilnius University

Aistė Skrebytė was the person at the Vilnius University Rector’s office that we could rely on. She helped our team with financial questions - team’s registration, Giant Jamboree-fee payments, and many others. She was always there for our team and tried to find best solutions to our organizational difficulties including our trip to Boston and general PR strategy.

Additional Thanks

Justinas Iljeitis

Who helped us with public relations and marketing at the beginning of the project.

Justina Žvirblytė

Who was involved in developing our idea as well as helped a lot with the InterLab study measurements.

Lina Bivainienė

Who supported us while planning the DNA day celebration.

Prof. Aurelija Žvirblienė

Who provided a theoretical background of antibody-antigen interactions.

Milda Zilnytė

Who helped us plan and perform scFv activity detection experiments.

Dr. Violeta Jonušienė

Who provided us with lab gear for SDS-PAGE.

Dovilė Strepetkaitė

Who provided us with a Precast Gel system and explained how to use it.

Prof. Christoph Flamm

Who surprised us with his willingness to teach us how to design custom RNA structures in silico and helped us create synthetic RNA thermometers.

Prof. Ivo Hofacker

Another renown specialist in RNA Bioinformatics, who dedicated his time and consulted us purely out of kind will on the design and applications of RNA thermoswitches.

Tadas Rimkus-Masaitis

Who kindly gave us a lot of valuable advice for problem-solving as an IT specialist.

Povilas Sidaravičius

Who enthusiastically mentored all the teams during the BioHackathon and inspired us to develop the idea of LipoVision.

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