Difference between revisions of "Team:Vilnius-Lithuania/Attributions"
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<h1>Additional Thanks</h1> | <h1>Additional Thanks</h1> | ||
| + | <strong>Audrius Žemys</strong> | ||
| + | <p>For organizing and hosting Lithuania’s first BioHackathon together with us.</p> | ||
| + | |||
| + | <strong>Justinas Iljeitis</strong> | ||
| + | <p>Who helped us with public relations and marketing at the beginning of the project.</p> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<strong>Justina Žvirblytė</strong> | <strong>Justina Žvirblytė</strong> | ||
| − | <p>Who was involved in developing our idea as well as helped a lot with the InterLab study measurements. | + | <p>Who was involved in developing our idea as well as helped a lot with the InterLab study measurements.</p> |
| − | </p> | + | |
| − | <strong>Lina Bivainienė | + | <strong>Lina Bivainienė</strong> |
| − | </strong> | + | <p>Who supported us while planning the DNA day celebration.</p> |
| − | <p>Who supported us while planning the DNA day celebration. | + | |
| − | </p> | + | <strong>Prof. Aurelija Žvirblienė</strong> |
| − | <strong>Prof. Aurelija Žvirblienė | + | <p>Who provided a theoretical background of antibody-antigen interactions.</p> |
| − | </strong> | + | |
| − | <p>Who provided a theoretical background of antibody-antigen interactions. | + | <strong>Milda Zilnytė</strong> |
| − | </p> | + | |
| − | <strong>Milda Zilnytė | + | |
| − | </strong> | + | |
<p>Who helped us plan and perform scFv activity detection experiments.</p> | <p>Who helped us plan and perform scFv activity detection experiments.</p> | ||
| − | <strong>Dr. Violeta Jonušienė | + | |
| − | </strong> | + | <strong>Dr. Violeta Jonušienė</strong> |
| − | <p>Who provided us with lab gear for SDS-PAGE. | + | <p>Who provided us with lab gear for SDS-PAGE.</p> |
| − | </p> | + | |
| − | <strong>Dovilė Strepetkaitė | + | <strong>Dovilė Strepetkaitė</strong> |
| − | </strong> | + | |
<p>Who provided us with a Precast Gel system and explained how to use it.</p> | <p>Who provided us with a Precast Gel system and explained how to use it.</p> | ||
| + | |||
<strong>Prof. Christoph Flamm</strong> | <strong>Prof. Christoph Flamm</strong> | ||
| − | <p>Who surprised us with his willingness to teach us how to design custom RNA structures in silico and helped us create synthetic RNA thermometers. | + | <p>Who surprised us with his willingness to teach us how to design custom RNA structures in silico and helped us create synthetic RNA thermometers.</p> |
| − | </p> | + | |
| − | <strong>Prof. Ivo Hofacker | + | <strong>Prof. Ivo Hofacker</strong> |
| − | </strong> | + | <p>Another renown specialist in RNA Bioinformatics, who dedicated his time and consulted us purely out of kind will on the design and applications of RNA thermoswitches.</p> |
| − | <p>Another renown specialist in RNA Bioinformatics, who dedicated his time and consulted us purely out of kind will on the design and applications of RNA thermoswitches. | + | |
| − | </p> | + | <strong>Tadas Rimkus-Masaitis</strong> |
| + | <p>Who kindly gave us a lot of valuable advice for problem-solving as an IT specialist.</p> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<strong>Povilas Sidaravičius</strong> | <strong>Povilas Sidaravičius</strong> | ||
| − | <p>Who enthusiastically mentored all the teams during the BioHackathon and inspired us to develop the idea of LipoVision. | + | <p>Who enthusiastically mentored all the teams during the BioHackathon and inspired us to develop the idea of LipoVision.</p> |
| + | |||
| + | <strong>Mindaugas Gapševičius</strong> | ||
| + | <p>Who helped us to plan and organize the “DIY-science” workshop.</p> | ||
| + | |||
| + | <strong>Juozas Nainys</strong> | ||
| + | <p>Who helped us accelerate the ordering processes of relevant reagents.</p> | ||
| + | |||
| + | <strong>Ignas Maželis</strong> | ||
| + | <p>Who helped us in the microfluidics’ laboratory and was involved in our idea’s development process.</p> | ||
| + | |||
| + | <strong>Dr. Karolis Leonavičius</strong> | ||
| + | <p>Who helped us with software maintenance of the syringe microfluidics station and contributed to BioHackathon related events.</p> | ||
| + | |||
| + | <strong>Antanas Žilakauskis</strong> | ||
| + | <p>Who helped us in developing our project’s idea.</p> | ||
| + | <strong>Matthijs Tadema</strong> | ||
| + | <p>Who kindly suggested to help us with molecular thermodynamics modeling of our surface display system.</p> | ||
Revision as of 19:51, 16 October 2018
Attributions
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Cell-free systems are becoming an increasingly popular in vitro tool to study biological processes as it is accompanied by less intrinsic and extrinsic noise. Relying on fundamental concepts of synthetic biology, we apply a bottom-up forward engineering approach to create a novel cell-free system for unorthodox protein-evolution. The core of this system is cell-sized liposomes that serve as excellent artificial membrane models. By encapsulating genetic material and full in vitro protein transcription and translation systems within the liposomes, we create reliable and incredibly efficient nanofactories for the production of target proteins. Even though there are many alternative proteins that can be synthesized, our main focus is directed towards membrane proteins, which occupy approximately one third of living-cells’ genomes. Considering their significance, membrane proteins are spectacularly understudied since synthesis and thus characterization of them remain prevailing obstacles to this day. We aim to utilize liposomes as nanofactories for directed evolution of membrane proteins. Furthermore, by means of directed membrane protein-evolution, a universal exposition system will be designed in order to display any protein of interest on the surface of the liposome. This way, a system is built where a phenotype of a particular protein is expressed on the outside while containing its genotype within the liposome. To prove the concept, small antibody fragments will be displayed to create a single-chain variable fragment (scFv) library for rapid screening of any designated target.
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