Difference between revisions of "Team:UPF CRG Barcelona/Improve"
| Line 76: | Line 76: | ||
<p class="page-title">IMPROVED PARTS</p> | <p class="page-title">IMPROVED PARTS</p> | ||
| − | <p><b>BBa_K2581011: | + | <p><b>BBa_K2581011: T14_pAR_32_RFP or Improved fatty acid acyl-CoA biosensor with medium RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | ||
acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA | acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA | ||
| Line 85: | Line 85: | ||
(BBa_E1010). | (BBa_E1010). | ||
</p> | </p> | ||
| − | <center><img src="/ | + | <center><img src="https://static.igem.org/mediawiki/2018/e/ec/T--UPF_CRG_Barcelona--goldenpart32.svg" style="width: 50%;"></center> |
| − | <p><b>BBa_K2581012: | + | <p><b>BBa_K2581012: T14_pAR_34_RFP or Improved fatty acid acyl-CoA biosensor with strong RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | ||
acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA | acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA | ||
| Line 94: | Line 94: | ||
(BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from <i>Discosoma striata</i> | (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from <i>Discosoma striata</i> | ||
(BBa_E1010). </p> | (BBa_E1010). </p> | ||
| − | <center><img src="/ | + | <center><img src="https://static.igem.org/mediawiki/2018/5/5c/T--UPF_CRG_Barcelona--goldenpart34.svg" style="width: 50%;"></center> |
</div> | </div> | ||
Revision as of 22:36, 16 October 2018
IMPROVED PARTS
BBa_K2581011: T14_pAR_32_RFP or Improved fatty acid acyl-CoA biosensor with medium RBS
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581012: T14_pAR_34_RFP or Improved fatty acid acyl-CoA biosensor with strong RBS
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), an strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
