Difference between revisions of "Team:UPF CRG Barcelona/Improve"

IMPROVED PARTS

BBa_K2581011: T14_pAR_32_RFP or Improved fatty acid acyl-CoA biosensor with medium RBS

The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.

This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).

BBa_K2581012: T14_pAR_34_RFP or Improved fatty acid acyl-CoA biosensor with strong RBS

The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.

This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), an strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).