Difference between revisions of "Team:UPF CRG Barcelona/Improve"
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<a class="navbar-logo" href="#"> | <a class="navbar-logo" href="#"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018"> | + | <img src="https://static.igem.org/mediawiki/2018/3/37/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018"> |
</a> | </a> | ||
<div class="navbar-burger" data-target="navbar-menu"> | <div class="navbar-burger" data-target="navbar-menu"> | ||
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<div class="swiper-wrapper"> | <div class="swiper-wrapper"> | ||
<a class="swiper-slide swiper-slide-menu" href="Basic.html"> | <a class="swiper-slide swiper-slide-menu" href="Basic.html"> | ||
| − | <embed src="https://static.igem.org/mediawiki/2018/T--UPF_CRG_Barcelona--basicpart.svg" alt=""> | + | <embed src="https://static.igem.org/mediawiki/2018/2/25/T--UPF_CRG_Barcelona--basicpart.svg" alt=""> |
<p>Basic Parts</p> | <p>Basic Parts</p> | ||
</a> | </a> | ||
<a class="swiper-slide swiper-slide-menu" href="Intermediate.html"> | <a class="swiper-slide swiper-slide-menu" href="Intermediate.html"> | ||
| − | <img style="height: 50px" src="https://2018.igem.org//wiki/images/T--UPF_CRG_Barcelona-- | + | <img style="height: 50px" src="https://2018.igem.org//wiki/images/falta/T--UPF_CRG_Barcelona--partsdeliverables.svg" alt=""> |
<p>Construction<br>Intermediates</p> | <p>Construction<br>Intermediates</p> | ||
</a> | </a> | ||
<a class="swiper-slide swiper-slide-menu" href="Composite.html"> | <a class="swiper-slide swiper-slide-menu" href="Composite.html"> | ||
| − | <embed src="https://static.igem.org/mediawiki/2018/T--UPF_CRG_Barcelona--compositepart.svg" alt=""> | + | <embed src="https://static.igem.org/mediawiki/2018/e/ed/T--UPF_CRG_Barcelona--compositepart.svg" alt=""> |
<p>Composite Parts</p> | <p>Composite Parts</p> | ||
</a> | </a> | ||
<a class="swiper-slide swiper-slide-menu" href="Improve.html"> | <a class="swiper-slide swiper-slide-menu" href="Improve.html"> | ||
| − | <embed src="https://static.igem.org/mediawiki/2018/T--UPF_CRG_Barcelona-- | + | <embed src="https://static.igem.org/mediawiki/2018/9/96/T--UPF_CRG_Barcelona--improvedpart.svg" alt=""> |
<p>Improved Parts</p> | <p>Improved Parts</p> | ||
</a> | </a> | ||
<a class="swiper-slide swiper-slide-menu" href="Registry.html"> | <a class="swiper-slide swiper-slide-menu" href="Registry.html"> | ||
| − | <embed src="https://static.igem.org/mediawiki/2018/T--UPF_CRG_Barcelona--registryparts.svg" alt=""> | + | <embed src="https://static.igem.org/mediawiki/2018/f/f5/T--UPF_CRG_Barcelona--registryparts.svg" alt=""> |
<p>Parts Registry</p> | <p>Parts Registry</p> | ||
</a> | </a> | ||
Revision as of 23:15, 16 October 2018
IMPROVED PARTS
BBa_K2581011: T14_pAR_32_RFP or Improved fatty acid acyl-CoA biosensor with medium RBS
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581012: T14_pAR_34_RFP or Improved fatty acid acyl-CoA biosensor with strong RBS
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), an strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
