Difference between revisions of "Team:Fudan/Primers"
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| − | <li><a href=" | + | <li><a href="./InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="./Notebook">Our notebook</a></li> |
| − | <li | + | <li>Primers used</li> |
| − | <li><a href=" | + | <li><a href="./Protocols">Protocols</a></li> |
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| − | < | + | <p>Primers</p> |
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| − | + | <p>gBlocks</p> | |
| − | + | <pre>> TEVp(FL)(CO) | |
| − | + | ATCACGTTAGGGCTAGTCGACGCCACCATGGGGGAGAGCCTGTTCAAAGGGCCCCGCGAT | |
| − | + | TATAACCCTATAAGCAGTACAATATGTCATCTGACTAACGAATCAGACGGACATACGACC | |
| − | + | TCCCTCTACGGGATAGGGTTCGGCCCCTTCATCATCACCAATAAGCACTTGTTTAGGCGA | |
| − | + | AATAACGGCACGCTTCTGGTACAGTCCCTGCACGGGGTCTTTAAGGTCAAGAATACGACA | |
| + | ACTCTCCAACAGCATCTTATTGATGGACGCGACATGATTATCATTAGAATGCCTAAAGAT | ||
| + | TTTCCGCCGTTCCCACAAAAATTGAAGTTTAGAGAACCGCAAAGGGAAGAGCGCATTTGT | ||
| + | TTGGTAACCACTAATTTTCAGACAAAGAGCATGTCCTCCATGGTTAGTGACACTTCATGC | ||
| + | ACGTTTCCTTCTTCTGATGGTATATTTTGGAAACATTGGATACAGACGAAAGACGGACAG | ||
| + | TGCGGGTCCCCTCTTGTCTCTACCAGGGACGGCTTTATCGTCGGCATACATTCAGCCAGT | ||
| + | AATTTCACAAACACAAATAATTATTTTACTAGCGTGCCCAAAAATTTCATGGAGTTGCTG | ||
| + | ACCAATCAAGAGGCACAGCAGTGGGTTTCTGGATGGAGACTCAACGCCGACAGCGTACTC | ||
| + | TGGGGTGGACATAAGGTGTTCATGgtgAAACCCGAGGAACCCTTTCAGCCGGTGAAAGAG | ||
| + | GCCACGCAACTGATGAACGAATTGGTCTATAGCCAGTGAAAGCTTCCCTCATTCAAGTAC</pre> | ||
| + | <pre>ZF21_16N-CfaN-CfaC-ZF21_16C(CO) | ||
| + | ATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGCGGACCGGATCAAAG | ||
| + | ACGCCGCCTCACGAACGACCATTTCAATGCAGGATCTGCATGCGGAACTTCTCCCGGAAC | ||
| + | TTTATCCTCCAGCGGCATACACGGACCCATACCGGAGAAAAGCCCTTCCAGTGCCTCTCA | ||
| + | TATGACACGGAAATTTTGACCGTCGAATATGGCTTTCTTCCTATCGGGAAGATCGTTGAA | ||
| + | GAAAGAATTGAATGCACGGTCTATACCGTGGATAAGAATGGTTTTGTATACACTCAACCG | ||
| + | ATAGCGCAGTGGCATAACAGGGGGGAGCAAGAAGTCTTTGAATACTGTTTGGAGGATGGG | ||
| + | TCCATCATCAGGGCAACCAAGGACCATAAGTTCATGACCACTGATGGGCAGATGCTTCCG | ||
| + | ATTGATGAAATTTTCGAAAGGGGCCTTGACCTGAAGCAGGTCGATGGGTTGCCAATGGTT | ||
| + | AAGATCATATCACGGAAGAGCCTGGGGACACAGAATGTGTATGATATTGGAGTAGGCGAG | ||
| + | CCGCACAACTTCCTCCTTAAAAATGGTCTCGTAGCTTCAAACTGTAGGATATGCATGCGA | ||
| + | AACTTCTCCCAGGGGGGCAACTTGGTCAGGCACCTTCGGACGCATACCGGAGAGAAGCCA | ||
| + | TTTCAGTGCAGGATTTGCATGCGAAATTTTTCTCAGCAAACCGGGTTGAATGTACATCTG | ||
| + | AAAACGCATACAGGTGAGAAGGGCTCCTGAAAGCTTCCCTCATTCAAGTAC</pre> | ||
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<!--Abstract on content page--> | <!--Abstract on content page--> | ||
Revision as of 11:06, 17 October 2018
Primers used
Primers
gBlocks
> TEVp(FL)(CO) ATCACGTTAGGGCTAGTCGACGCCACCATGGGGGAGAGCCTGTTCAAAGGGCCCCGCGAT TATAACCCTATAAGCAGTACAATATGTCATCTGACTAACGAATCAGACGGACATACGACC TCCCTCTACGGGATAGGGTTCGGCCCCTTCATCATCACCAATAAGCACTTGTTTAGGCGA AATAACGGCACGCTTCTGGTACAGTCCCTGCACGGGGTCTTTAAGGTCAAGAATACGACA ACTCTCCAACAGCATCTTATTGATGGACGCGACATGATTATCATTAGAATGCCTAAAGAT TTTCCGCCGTTCCCACAAAAATTGAAGTTTAGAGAACCGCAAAGGGAAGAGCGCATTTGT TTGGTAACCACTAATTTTCAGACAAAGAGCATGTCCTCCATGGTTAGTGACACTTCATGC ACGTTTCCTTCTTCTGATGGTATATTTTGGAAACATTGGATACAGACGAAAGACGGACAG TGCGGGTCCCCTCTTGTCTCTACCAGGGACGGCTTTATCGTCGGCATACATTCAGCCAGT AATTTCACAAACACAAATAATTATTTTACTAGCGTGCCCAAAAATTTCATGGAGTTGCTG ACCAATCAAGAGGCACAGCAGTGGGTTTCTGGATGGAGACTCAACGCCGACAGCGTACTC TGGGGTGGACATAAGGTGTTCATGgtgAAACCCGAGGAACCCTTTCAGCCGGTGAAAGAG GCCACGCAACTGATGAACGAATTGGTCTATAGCCAGTGAAAGCTTCCCTCATTCAAGTAC
ZF21_16N-CfaN-CfaC-ZF21_16C(CO) ATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGCGGACCGGATCAAAG ACGCCGCCTCACGAACGACCATTTCAATGCAGGATCTGCATGCGGAACTTCTCCCGGAAC TTTATCCTCCAGCGGCATACACGGACCCATACCGGAGAAAAGCCCTTCCAGTGCCTCTCA TATGACACGGAAATTTTGACCGTCGAATATGGCTTTCTTCCTATCGGGAAGATCGTTGAA GAAAGAATTGAATGCACGGTCTATACCGTGGATAAGAATGGTTTTGTATACACTCAACCG ATAGCGCAGTGGCATAACAGGGGGGAGCAAGAAGTCTTTGAATACTGTTTGGAGGATGGG TCCATCATCAGGGCAACCAAGGACCATAAGTTCATGACCACTGATGGGCAGATGCTTCCG ATTGATGAAATTTTCGAAAGGGGCCTTGACCTGAAGCAGGTCGATGGGTTGCCAATGGTT AAGATCATATCACGGAAGAGCCTGGGGACACAGAATGTGTATGATATTGGAGTAGGCGAG CCGCACAACTTCCTCCTTAAAAATGGTCTCGTAGCTTCAAACTGTAGGATATGCATGCGA AACTTCTCCCAGGGGGGCAACTTGGTCAGGCACCTTCGGACGCATACCGGAGAGAAGCCA TTTCAGTGCAGGATTTGCATGCGAAATTTTTCTCAGCAAACCGGGTTGAATGTACATCTG AAAACGCATACAGGTGAGAAGGGCTCCTGAAAGCTTCCCTCATTCAAGTAC
Abstract
Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.