Difference between revisions of "Team:EPFL/Improve"

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<h3>BBa_K2696005</h3>
 
<h3>BBa_K2696005</h3>
  
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<a href="http://parts.igem.org/Part:BBa_K192000">BBa_K192000</a> is a part that was brought to the registry by iGEM Toronto 2009. We designed an upgraded part, <a href="http://parts.igem.org/Part:BBa_K2686005">BBa_K2686005</a> with a different encapsulin sequence where we introduced a HexaHistidine loop on the protein subunit's inner surface which increases the heat resistance and stability of the part. To facilitate the introduction of antigen encoding sequences into the HexaHistidine Encapsulin, BsaI cut sites are present on the Ecapsulin's C terminus with an insert coding for sfGFP under a native promoter to enable selection of successful transformants by color.
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<a href="http://parts.igem.org/Part:BBa_K192000">BBa_K192000</a> is a part that was brought to the registry by iGEM Toronto 2009. We designed an upgraded composite part, <a href="http://parts.igem.org/Part:BBa_K2686005">BBa_K2686005</a> with a different encapsulin sequence where we introduced a HexaHistidine loop on the protein subunit's inner surface which increases the heat resistance and stability of the part. To facilitate the introduction of antigen encoding sequences into the HexaHistidine Encapsulin, BsaI cut sites are present on the Ecapsulin's C terminus with an insert coding for sfGFP under a native promoter to enable selection of successful transformants by color.
  
  

Revision as of 12:01, 17 October 2018

iGEM EPFL 2018

Improve

Introduction

Encapsulins are versatile proteins found in a variety of different bacteria (Giessen and Silver, 2017). In the case of this specific part derived from Thermotoga maritima, it can be used among other things to deliver cargo, both on the outer surface of the cage by fusing a peptide in between the 139/140 Amino Acids or the protein's C terminus. A cargo protein can also be loaded by fusing it with a tag binding to Encapsulin's interior surface (Cassidy-Amstutz et al., 2016).

Improve

For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.

Gold Medal Criterion #2

Standard Tracks: Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement. Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry. The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.

BBa_K2696005

BBa_K192000 is a part that was brought to the registry by iGEM Toronto 2009. We designed an upgraded composite part, BBa_K2686005 with a different encapsulin sequence where we introduced a HexaHistidine loop on the protein subunit's inner surface which increases the heat resistance and stability of the part. To facilitate the introduction of antigen encoding sequences into the HexaHistidine Encapsulin, BsaI cut sites are present on the Ecapsulin's C terminus with an insert coding for sfGFP under a native promoter to enable selection of successful transformants by color.

Special Tracks: Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.