Difference between revisions of "Team:UPF CRG Barcelona/Improve"
| Line 77: | Line 77: | ||
<p class="page-title">IMPROVED PARTS</p> | <p class="page-title">IMPROVED PARTS</p> | ||
| − | <p><b>BBa_K2581011: | + | <p><b>BBa_K2581011: Improved fatty acid acyl-CoA biosensor with medium RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | ||
acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA | acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA | ||
| Line 89: | Line 89: | ||
<center><img src="https://static.igem.org/mediawiki/2018/e/ec/T--UPF_CRG_Barcelona--goldenpart32.svg" style="width: 50%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/e/ec/T--UPF_CRG_Barcelona--goldenpart32.svg" style="width: 50%;"></center> | ||
<div class="spacer"></div> | <div class="spacer"></div> | ||
| − | <p><b>BBa_K2581012: | + | <p><b>BBa_K2581012: Improved fatty acid acyl-CoA biosensor with strong RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | ||
acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA | acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA | ||
Revision as of 14:51, 17 October 2018
IMPROVED PARTS
BBa_K2581011: Improved fatty acid acyl-CoA biosensor with medium RBS
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581012: Improved fatty acid acyl-CoA biosensor with strong RBS
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). It act as an intracellular biosensor of fatty acid acyl-coA but in this case the high basality activity of the promoter has been reduced.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pFadBA DNA sequence (BBa_K2581013), an strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
