Difference between revisions of "Team:Munich"
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<div class="row align-items-center justify-content-end how-it-works d-flex"> | <div class="row align-items-center justify-content-end how-it-works d-flex"> | ||
<div class="col-8 col-md-6 text-center"> | <div class="col-8 col-md-6 text-center"> | ||
| − | <h5>Phage Selection</h5> | + | <h5>Phage Library Selection</h5> |
<p class="text-justify">By using a cell-free production system, we utilize one of the most revolutionary concepts of synthetic biology to produce therapeutic bacteriophages. Based on a personalized diagnosis, bacteriophage DNA is chosen from a library and is subjected to extensive quality assessment procedures to ensure a safe and effective application.</p> | <p class="text-justify">By using a cell-free production system, we utilize one of the most revolutionary concepts of synthetic biology to produce therapeutic bacteriophages. Based on a personalized diagnosis, bacteriophage DNA is chosen from a library and is subjected to extensive quality assessment procedures to ensure a safe and effective application.</p> | ||
<div class="labelBox"> | <div class="labelBox"> | ||
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<span>✖</span> | <span>✖</span> | ||
<h1>homogeneous</h1> | <h1>homogeneous</h1> | ||
| − | <p>Because our raw material is DNA, phage therapy based on well defined phages is no longer a fantasy.</p> | + | <p>Because our raw material is DNA, phage therapy based on well-defined phages is no longer a fantasy.</p> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="text-center my-4"> | <div class="text-center my-4"> | ||
| − | <a href="https://2018.igem.org/Team:Munich/ | + | <a href="https://2018.igem.org/Team:Munich/Description" class="readButton btn btn-outline-info mt-2">Read More</a> |
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</div> | </div> | ||
<div class="col-8 col-md-6 text-center"> | <div class="col-8 col-md-6 text-center"> | ||
| − | <h5>Phage Manufacturing</h5> | + | <h5>Synthetic Phage Manufacturing</h5> |
<p class="text-justify">Production of our bacteriophages in a cell-free system ensures a fast and dependable application. Furthermore, working in a cell-free environment enables host independent production of phages in only 2.5% of the traditional production volume while demanding no biosafety regulations.</p> | <p class="text-justify">Production of our bacteriophages in a cell-free system ensures a fast and dependable application. Furthermore, working in a cell-free environment enables host independent production of phages in only 2.5% of the traditional production volume while demanding no biosafety regulations.</p> | ||
<div class="labelBox"> | <div class="labelBox"> | ||
| − | <button class="reg"> | + | <button class="reg"> affordable </button> |
<div class="pop text-left"> | <div class="pop text-left"> | ||
<span>✖</span> | <span>✖</span> | ||
<h1>affordable</h1> | <h1>affordable</h1> | ||
| − | <p>Below $10 per treatment, Phactory has a very competitive market value</p> | + | <p>Below $10 per treatment, Phactory has a very competitive market value.</p> |
</div> | </div> | ||
</div> | </div> | ||
<span> | </span> | <span> | </span> | ||
<div class="labelBox"> | <div class="labelBox"> | ||
| − | <button class="reg"> | + | <button class="reg"> scalable </button> |
<div class="pop text-left"> | <div class="pop text-left"> | ||
<span>✖</span> | <span>✖</span> | ||
<h1>scalable</h1> | <h1>scalable</h1> | ||
| − | <p>Manufacturing in Phactory can be scaled up for mass production</p> | + | <p>Manufacturing in Phactory can be scaled up for mass production.</p> |
</div> | </div> | ||
</div> | </div> | ||
<span> | </span> | <span> | </span> | ||
<div class="labelBox"> | <div class="labelBox"> | ||
| − | <button class="reg"> | + | <button class="reg"> modular</button> |
<div class="pop text-left"> | <div class="pop text-left"> | ||
<span>✖</span> | <span>✖</span> | ||
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<span>✖</span> | <span>✖</span> | ||
<h1>safe</h1> | <h1>safe</h1> | ||
| − | <p> | + | <p>Host independence eliminates the need to cultivate pathogens to produce bacteriophages.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="text-center my-4"> | <div class="text-center my-4"> | ||
| − | <a href="https://2018.igem.org/Team:Munich/ | + | <a href="https://2018.igem.org/Team:Munich/Description" class="readButton btn btn-outline-info mt-2">Read More</a> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="row align-items-center justify-content-end how-it-works d-flex"> | <div class="row align-items-center justify-content-end how-it-works d-flex"> | ||
<div class="col-8 col-md-6 text-center"> | <div class="col-8 col-md-6 text-center"> | ||
| − | <h5> | + | <h5>Quality Control</h5> |
| − | <p class="text-justify"> | + | <p class="text-justify">Extensive quality controls are employed to make sure that the produced bacteriophages are pure and effective. Potential complications due to toxins and contaminants are eliminated.</p> |
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<button class="reg">pure</button> | <button class="reg">pure</button> | ||
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<span>✖</span> | <span>✖</span> | ||
<h1>pure</h1> | <h1>pure</h1> | ||
| − | <p>Purity is essential for any therapeutic application. | + | <p>Purity is essential for any therapeutic application. We use next-generation sequencing to ensure the absence of any contaminating DNA in the manufacturing process.</p> |
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<div class="text-center my-4"> | <div class="text-center my-4"> | ||
| − | <a href="https://2018.igem.org/Team:Munich/ | + | <a href="https://2018.igem.org/Team:Munich/Description" class="readButton btn btn-outline-info mt-2">Read More</a> |
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<p class="text-justify">Bacteriophage therapy covers a broad range of potential applications. We engineered custom hardware to package bacteriophages for oral treatment. Our packaging procedure is the last step of a personalized on-site treatment process.</p> | <p class="text-justify">Bacteriophage therapy covers a broad range of potential applications. We engineered custom hardware to package bacteriophages for oral treatment. Our packaging procedure is the last step of a personalized on-site treatment process.</p> | ||
<div class="labelBox"> | <div class="labelBox"> | ||
| − | <button class="reg">shelf-life</button> | + | <button class="reg">long shelf-life</button> |
<div class="pop text-left"> | <div class="pop text-left"> | ||
<span>✖</span> | <span>✖</span> | ||
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<span> | </span> | <span> | </span> | ||
<div class="labelBox"> | <div class="labelBox"> | ||
| − | <button class="reg"> | + | <button class="reg">portable</button> |
<div class="pop text-left"> | <div class="pop text-left"> | ||
<span>✖</span> | <span>✖</span> | ||
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<span> | </span> | <span> | </span> | ||
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| − | <button class="reg"> | + | <button class="reg">on-site</button> |
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<span>✖</span> | <span>✖</span> | ||
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</div> | </div> | ||
<div class="text-center my-4"> | <div class="text-center my-4"> | ||
| − | <a href="https://2018.igem.org/Team:Munich/ | + | <a href="https://2018.igem.org/Team:Munich/Hardware" class="readButton btn btn-outline-info mt-2">Read More</a> |
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</div> | </div> | ||
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<div class="col-8 col-md-6 text-center"> | <div class="col-8 col-md-6 text-center"> | ||
<h5>Therapy</h5> | <h5>Therapy</h5> | ||
| − | <p class="text-justify">Our bacteriophages are now ready for therapy. Phactory is fast and reliable enough to be | + | <p class="text-justify">Our bacteriophages are now ready for therapy. Phactory is fast and reliable enough to be an oral bedside application.</p> |
<div class="labelBox"> | <div class="labelBox"> | ||
<button class="reg">cooperation</button> | <button class="reg">cooperation</button> | ||
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<div class="text-center my-4"> | <div class="text-center my-4"> | ||
| − | <a href="https://2018.igem.org/Team:Munich/ | + | <a href="https://2018.igem.org/Team:Munich/Human_Practices" class="readButton btn btn-outline-info mt-2">Read More</a> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 17:06, 17 October 2018
Diagnosis
Next generation diagnostic systems like SHERLOCK can identify pathogens with high precision and accuracy. This enables rapid identification of an individualized therapeutic strategy even in the case of multiresistant superbugs. Using Phactory, we overcome the last remaining obstacles for an efficient bacteriophage therapy.
Phage Library Selection
By using a cell-free production system, we utilize one of the most revolutionary concepts of synthetic biology to produce therapeutic bacteriophages. Based on a personalized diagnosis, bacteriophage DNA is chosen from a library and is subjected to extensive quality assessment procedures to ensure a safe and effective application.
homogeneous
Because our raw material is DNA, phage therapy based on well-defined phages is no longer a fantasy.
software-aided
State of the art DNA sequencing tools are combined with bioinformatics to precisely determine the amount of contaminants in a sample.
high quality
The phage genome DNA is produced using extensive purification protocols, resulting in high quality raw materials with minimal contamination.
Synthetic Phage Manufacturing
Production of our bacteriophages in a cell-free system ensures a fast and dependable application. Furthermore, working in a cell-free environment enables host independent production of phages in only 2.5% of the traditional production volume while demanding no biosafety regulations.
affordable
Below $10 per treatment, Phactory has a very competitive market value.
scalable
Manufacturing in Phactory can be scaled up for mass production.
modular
Phactory is an open system. This allows for the incorporation of modified proteins while leaving the genome unmodified, thereby achieving modularity without GMOs.
safe
Host independence eliminates the need to cultivate pathogens to produce bacteriophages.
Quality Control
Extensive quality controls are employed to make sure that the produced bacteriophages are pure and effective. Potential complications due to toxins and contaminants are eliminated.
pure
Purity is essential for any therapeutic application. We use next-generation sequencing to ensure the absence of any contaminating DNA in the manufacturing process.
toxin-free
Phactory assembles phages outside of living cells and is therefore free of most biological contaminants. We engineered it to reduce immunogenicity in humans by five orders of magnitude.
GMO-free
The phages assembled by Phactory are purified to remove any genetically modified material before ever leaving a controlled lab environment.
Packaging
Bacteriophage therapy covers a broad range of potential applications. We engineered custom hardware to package bacteriophages for oral treatment. Our packaging procedure is the last step of a personalized on-site treatment process.
long shelf-life
Phactory and phages are fragile components. We combined various techniques to enable phage assembly outside of a lab.
portable
We demonstrated lyophilization of Phactory components in order to enable long range transport.
on-site
Producing phages in Phactory is so easy that it allows any doctor to apply phage therapy on-site.
Therapy
Our bacteriophages are now ready for therapy. Phactory is fast and reliable enough to be an oral bedside application.
cooperation
We were able to produce genetically modified phages we received from the iGEM Team in Grenoble.
integrated human practice
We reached out to local doctors to demonstrate just how easy bacteriophage therapy using Phactory is and to gain valuable input for our project.
ready
In cooperation with phage therapy pioneers in Belgium, we safely produced functional phages against EHEC – a severe intestinal infection.
Teams
Participants
International speakers
Workshops
