Difference between revisions of "Team:Marburg"
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| − | Establishing Vibrio natriegens as the new chassis organism for synthetic biology | + | Establishing <i>Vibrio natriegens</i> as the new chassis organism for synthetic biology |
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| − | We developed a workflow for accelerating metabolic engineering. As a proof of concept we established the first synthetic pathway in Vibrio natriegens to produce the platform chemical 3-hydroxypropionic acid. | + | We developed a workflow for accelerating metabolic engineering. As a proof of concept we established the first synthetic pathway in <i>Vibrio natriegens</i> to produce the platform chemical 3-hydroxypropionic acid. |
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| − | To collaborate with other teams and spread the word to other scientists on Vibrio natriegens' advantages we had teams from all over Europe conduct a growth curve or a InterLab inspired experiment. | + | To collaborate with other teams and spread the word to other scientists on <i>Vibrio natriegens'</i> advantages we had teams from all over Europe conduct a growth curve or a InterLab inspired experiment. |
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| − | <td> | + | <td>Initially, we carried out foundational experiments to characterize the growth rate, pH, salt and antibiotic tolerance of <i>V. natriegens</i> and sequenced its genome. |
| + | </td> | ||
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>We performed Cloning in One day: From transformation to isolated plasmids in less than 12 hours!</td> |
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Low amounts of DNA could be transformed reliably and we successfully demonstrated challenging clonings such as Gibson Assembly with 5 and Aquacloning with 3 fragments as well as 8 part golden-gate reactions</td> |
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>We designed and constructed the Marburg Collection, a highly flexible golden-gate based toolbox consisting of 123 parts |
| + | </td> | ||
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Dozens of test constructs were built and tested to obtain characterization data for all part categories in our toolbox |
| + | </td> | ||
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>This was only possible with our novel experimental and data analysis workflow using platereader measurements |
| + | </td> | ||
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>We successfully demonstrated a genome engineering workflow in <i>V. natriegens</i> to establish three new lab strains for diverse applications. |
| + | </td> | ||
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Subsequently, we were able to use Flp/FRT recombinase system for marker recycling thus allowing additional rounds of genomic modifications</td> |
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
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| − | <td> | + | <td>Chromosomal locations suitable for genomic integration were identified and characterized to detect possible fitness effects </td> |
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>We accelerated metabolic engineering by developing a workflow for rapid pathway assembly and pathway optimization in <i>V. natriegens</i></td> |
| + | <td><div class="checkmark"></div></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Model-predicted parts were used to construct a pathway for maximal 3-hydroxypropionate production in <i>V. natriegens</i> and we demonstrated its functionality</td> | ||
| + | <td><div class="checkmark"></div></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Via mass spectrometry, we detected our pathway product 3-hydroxypropionic acid in <i>V. natriegens</i> | ||
| + | </td> | ||
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
Revision as of 19:33, 17 October 2018
Strain Engineering
Part Collection
Metabolic Engineering
Interlab
Accessible Science
