Difference between revisions of "Team:WLC-Milwaukee/Experiments"
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| − | <p>Our Wet Lab efforts focused on first designing our parts in such a way as to test new versions of past WLC-iGEM binding proteins for optimization and use in our test kit. Our work then turned to successfully sub-cloning our new parts (BBa_K2589000, BBa_K2589002) from the pETail4 plasmid containing the lambda phage genome into both pTrc99a and pSB1C3 along with improving a past part BBa_K2452002 to create the composite part BBa_K2589001. Our final focus in WetLab was to purify the proteins from our new parts using Nickel column purification (as most of our parts contain a His tag element) and then confirming expression or protein presence. </p> | + | <p>Our Wet Lab efforts focused on first designing our parts in such a way as to test new versions of past WLC-iGEM binding proteins for optimization and use in our test kit. Our work then turned to successfully sub-cloning our new parts (<a href="http://parts.igem.org/Part:BBa_K2589000">BBa_K2589000</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2589002">BBa_K2589002 |
| + | </a>) from the pETail4 plasmid containing the lambda phage genome into both pTrc99a and pSB1C3 along with improving a past part <a href="http://parts.igem.org/Part:BBa_K2452002">BBa_K2452002</a> to create the composite part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2589001">BBa_K2589001</a>. Our final focus in WetLab was to purify the proteins from our new parts using Nickel column purification (as most of our parts contain a His tag element) and then confirming expression or protein presence. </p> | ||
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Revision as of 17:38, 17 October 2018
Wet Lab
Our Wet Lab efforts focused on first designing our parts in such a way as to test new versions of past WLC-iGEM binding proteins for optimization and use in our test kit. Our work then turned to successfully sub-cloning our new parts (BBa_K2589000, BBa_K2589002 ) from the pETail4 plasmid containing the lambda phage genome into both pTrc99a and pSB1C3 along with improving a past part BBa_K2452002 to create the composite part BBa_K2589001. Our final focus in WetLab was to purify the proteins from our new parts using Nickel column purification (as most of our parts contain a His tag element) and then confirming expression or protein presence.
Cloning
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in Protocol Link 1 reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla Protocol Link 2 pariatur. Excepteur sint occaecat cupidatat non proident, sunt in Protocol Link 3 culpa qui officia deserunt mollit a nim id est laborum.
Protein Expression
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in Protocol Link 4 reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in Protocol Link 5 culpa qui officia deserunt mollit a nim id est laborum.
