Revision as of 17:41, 17 October 2018

Primers used
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Primers used

... they are a lot of $$$

Primers used

... they are a lot of $$$

Primers

#	Oligo Name	DNA Sequence (5 to 3)
O1	pLL7.1-F(S)	GGTCTATATAAGCAGAGCTGG
O2	pLL7.1-R(S)	AACCGGAACCCTTAAACATG
O3	LaG16-F(G_pLL7.1)	TGAACCGTCAGATCCGCTAGAATTCGCCACCATGGCCCTGCCCGTGACC
O4	LaG16-R(G_mNc)	AGTCCAGGATGCTCACGGTCACCTGGGTG
O5	mNc-F(G_LaG16)	GACCGTGAGCATCCTGGACTACAGCTTCACAGGTGGCGC
O6	mNc\|e-R(G_tTa)	ATCTAGACATCCGCCGGCGCTTGCGGGA
O7	tTa-F(G_mNc\|e)	GCGCCGGCGGATGTCTAGATTAGATAAAAGTAAAGTGATTAAC
O8	tTa-R(G_pLL7.1)	CCCTCGACGAATTGCCTGCAGGCTACCCACCGTACTCGTCAATTC
O9	LaG16-R(G-mNc(e))	CCACACAGGGGCTCACGGTCACCTGGGTG
O10	mNc(e)-F(G_LaG16)	GACCGTGAGCCCCTGTGTGGGTAGCAACCCCTGCTAC
O11	mNotch 1 core-PF	ATCCTGGACTACAGCTTCACAG
O12	mNotch 1 core (extended)-PF	CCCTGTGTGGGTAGCAACC
O13	mNotch 1 core-PB	CCGCCGGCGCTTG
O14	LaG16-F(EcoRI-Kozark)	GCTGAATTCGCCACCATGGCCCTGCCCGTG
O15	LaG16-B(OE	mNotch)	CTGTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGG
O16	LaG16-B(OE	mNCe)	GGTTGCTACCCACACAGGGGCTCACGGTCACCTGGG
O17	mcherry-F(OE	mNotch)	CAAGCGCCGGCGGATGGTGAGCAAGGGCGAG
O18	mCherry-B(UAA-HindIII)	GCTAAGCTTTTACTTGTACAGCTCGTCCATGC
O19	CMV-F(ala-EcoRI-ala-XbaI)	GCAGAATTCGCATCTAGAGACATTGATTATTGACGAGTTATTAATAGTAATCAATTACG
O20	LacOi-F(OE	intron)	CGTAATTGTGAGCGGATAACAATTACGTTCGCCCCGTGCCCCG
O21	LacOi-B(OE	intron)	CGTAATTGTTATCCGCTCACAATTACGGGCAACGCAGCGACTC
O22	intron-B(SpeI-ala-HindIII-ala)	GCAAAGCTTGCAACTAGTCTGTAGGAAAAAGAAGAAGGCATGAAC
O23	LaG16-F(G_pML2-EcoRI-Kozark)	AGTTATTAGGTCCCTCGACGAATTCGCCACCATGGCCCTGCCCGTGACC
O24	LaG16-R(G_mNC)	GTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGGTG
O25	LaG16-R(G_mNCe)	GGGTTGCTACCCACACAGGGGCTCACGGTCACCTGGGTG
O26	mcherry-F(G_mNC)	TGTCCCGCAAGCGCCGGCGGATGGTGAGCAAGGGCGAG
O27	mcherry-R(HindIII	G_pML2)	AAGTTATATTAAGGGTTCCAAGCTTTTACTTGTACAGCTCGTCCATG
O28	rev_41BB_CD3z9	序列缺失
O29	hNotch-4168	CCCTGCCTGGGCGGCAAC
O30	5286-hNotch	CCGCCGGCGCTTGCGGG
O31	tTA-start	CAAGCGCCGGCGG ATGTCTAGATTAGATAAAAGTAAAGTG
O32	rev_tTA-end-3NotI-2-Hind3-5	GTTCCAAGCTTAAGCGCTACCCACCGTACTCG
O33	EGFP_BglⅡ_F	GCAGATCTatggtgagcaagggcga
O34	EGFP_XbalI_R	GCTCTAGActttacttgtacagctcgtccat
O35	rtTA_pLL7.0_F	TGAACCGTCAGATCCGCTAGAAGTTTGTACAAAAAAGCAG
O36	rtTA_EGFP_R	TGGCACTTTTTTTAAATGATTTGCCCTCCC
O37	rtTa_EGFP_F	GCAAATCATTTAAAAAAAGTGCCACCTGACGTC
O38	LacI_pLL7.0_R	GCAAATCATTTAAAAAAAGTGCCACCTGACGTC
O39	lacI_mFF4_ployA_F	TACCGCGGGCCCGGGATCCATTTGACAATTAAAGCTTGAGG
O40	mFF4_ployA_pCAGop_R	attaataactaGCGCAGCGAGTCAGTGAG
O41	mFF4_polyA_pCAGop_F	ctcgctgcgcTAGTTATTAATAGTAATCAATTACGGG
O42	pCAGop_pLL7.0_R	CTTGCTCACCATGGTGGCGACTGTAGGAAAAAGAAGAAGG
O43	mFF4_pll7.0_R	CTTGCTCACCATGGTGGCGAGCGCAGCGAGTCAGTGAG
O44	TRE_pLL7.0_R	cgcggtaccgtcgactgcagTACCGAGCTCGAAATCTC
O45	TRE_GAL4_F	TACCGCGGGCCCGGGATCCAAAGTTTGTACAAAAAAGCAG
O46	GAL4_UAS5_R	TCACATGAGATACAGTCAACTGTCTTTG
O47	GAS4_UAS5_F	gttgactgtaTCTCATGTGATTACGCCAAG
O48	UAS5_mCherry_R	cttgctcaccatggtggcgaTGCTTTTTTGTACAAACTTGG
O49	pLL7.0_mCherry_F	aagcttatcgataatcaacctctggattacaaaatttgtgaaaga
O50	pLL7.0_mCherry_R	GATATCGAATTGTACCTAGTGGAACCGGAACC
O51	mCherry_FF4_polyA_F	actaggtacaattcgatatcGAATTCTGCAGTCGACGG
O52	FF4_ployA_pLL7.0_R	ggttgattatcgataagcttTTTGCCCTCCCATATGTC
O53	EGFPpoly(A)F	agtttggttagtaccgcgctGCAAATCATTTAAAAAAAGTGCCACC
O54	EGFPpoly(A)R	cctggagaatgccaccATGGTGAGCAAGGGCGAG
O55	Bi_TRE F	tgctcaccatggtggcATTCTCCAGGCGATCTGACGG
O56	Bi_TRE R	cgcggtaccgtcgactgcaggaattcCGCGGTACCGTCGACTGC
O57	pll7.0.1 F	cagggatattcaccattatcgtttcagacc
O58	poly(A)F	cgacggtaccgcgggcccggAACTTGTTTATTGCAGCTTATAATG
O59	poly(A)R	caccatggtggcgaccggtgGCAGTGAAAAAAATGCTTTATTTG
O60	BiTRE F	ccatggtggcattctccaggGCCTCAGGAGATCTcAATTCtcc
O61	BiTRE R	cgcggtaccgtcgactgcagaattctaccgagctcgaaatctcca
O62	pSB1C3-VR	ATTACCGCCTTTGAGTGAGC
O63	pSB1C3-VF	TGCCACCTGACGTCTAAGAA
O64	pSB1C3-BP	GAATTCGCGGCCGCTTC
O65	pSB1C3-BS	CTGCAGCGGCCGCTAC
O66	EGFP-F(G_pQM6.2)	gcaagagcaagggccaattgGTGAGCAAGGGCGAGGAG
O67	EGFP-R(G_P2A)	gtcgacccCTTGTACAGCTCGTCCATGC
O68	P2A-F(G_EGFP)	ctgtacaagGGGTCGACGGGCTCCGGC
O69	mcherry-R(G_pQM6.2)	ccctttttctggagacaagcttTTACTTGTACAGCTCGTCCATGCCG
O70	pQM6.2-R(L-P2A)	ggatctggggccggggttctcc
O71	pQM6.2-F(L-EGFP)	atggtgagcaagggcgaggagc
O72	alpha-SP-LaG16-F(3-AgeI)	GCAACCGGTGCCACCATGGCCCTGC
O73	tTa-R(HindIII-3)	GCAAAGCTTCTACCCACCGTACTCGTCAATTCc
O74	P-minCMV-F	GAATTCGCGGCCGCTTCTAGgaggtaggcgtgtacggtg
O75	S-minCMV-R	CTGCAGCGGCCGCTACTAGTAggcgatctgacggttcactaaac
O76	P-pSV40-F	GAATTCGCGGCCGCTTCTAGgtgtgtcagttagggtgtgg
O77	S-pSV40-R	CTGCAGCGGCCGCTACTAGTAtttgcaaaagcctaggcctccaaaaaag
O82	SV40NLS-KRAB-F	CCAAAAAAGAAGAGAAAGGTAatggacgccaagagcctg
O83	BS-F	tactagtagcggccgctgc
O84	BP-R	ctctagaagcggccgcg
O85	KRAB-R	caccagccagggttcctc
O86	mcherry-F	ATGGTGAGCAAGGGCGAGGAGGATAACATG
O87	pTight-F(S)	AGCTCGTTTAGTGAACCGTCAG
O88	3 LTR (Delta-U3)-R(S)	GCGTTACTTAAGCTAGCTTGCC
O89	P2A-F(S)	ACCAACTTCTCCCTGCTGAAG
O90	mcherry-R(S)	CATGTGCACCTTGAAGCGC
O91	BP protect	tttctggaattcgcggccg
O92	BS protect	gccggactgcagcg
O93	2* ZF 42-10 operator	GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a GAC GCT GCT c TACTAGTAGCGGCCGCTGCAG
O94	2* ZF 54-8 operator	GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a TGG GTG GCA t TACTAGTAGCGGCCGCTGCAG
O95	2* ZF 43-8 operator	GAATTCGCGGCCGCTTCTAG a GAG TGA GGA c a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG
O96	2* ZF 21-16 operator	GAATTCGCGGCCGCTTCTAG a TTA GAA GTG a a TTA GAA GTG a TACTAGTAGCGGCCGCTGCAG
O97	1* ZF 42-10 & 54-8 operator	GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a TGG GTG GCA t TACTAGTAGCGGCCGCTGCAG
O98	1* ZF 42-10 & 43-8 operator	GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG
O99	1* ZF 54-8 & 43-8 operator	GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG
O100	2* ZFHD1	GAATTCGCGGCCGCTTCTAG TAATGATGGGCG TA TAATGATGGGCGTATAATGATGGGCGTATAATGATGGGCG TA TACTAG
		TAGCGGCCGCTGCAG
O101	1*PIR	GAATTCGCGGCCGCTTCTAGGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACTACTAGTAGCGGCCGCTGCAG
O102	pCMV-F(S)	cgtgtacggtgggaggtctatataag
O103	pML2-R(S)	acgagattccagcaggtcg
O106	pSB1C3-BP(L)	tttctggaattcgcggccgcttc
O107	pSB1C3-BS(L)	gccggactgcagcggccgc
O108	PIR-F(6-XbaI)	GCCGCTTCTAGagGAAATAGCGCTGTACAGCG
O109	2*ZFHD1-F(6-XbaI)	GCCGCTTCTAGagTAATGATGGGCGTATAATGATGG
O110	CD8-alpha-SP-F(G_pML2)	gatccgctagcgctaccggtgccaccATGGCCTTACCAGTGACCGCCTTG
O111	alpha-GPC3-R(G_mNce)	acacagggTGGTCGCGGCGCTGGCGT
O112	mNce-F(G_alpha-GPC3)	cgcgaccaCCCTGTGTGGGTAGCAACC
O113	tTa-R(G_pML2)	gttatattaagggttccaagcttCTACCCACCGTACTCGTCAATTC
O114	alpha-GPC3-R(G_mNc)	TCCAGGATTGGTCGCGGCGCTGGCGT
O115	mNc-F(G_alpha-GPC3)	cgcgaccaATCCTGGACTACAGCTTCACAG
O116	CD8-alpha-F2(G_pML2)	GATCCGCTAGCGCTACCGGTGCCACCATGGCCCTGCCCGTGACC
O117	CD8-alpha-SP-F(G_pML2)	cgtcagatccgctagcgctaccggtGCCACCATGGCCCTGCCCG
O118	KRAB-R(G_pML2)	aagttatattaagggttccaagctttcaCACCAGCCAGGGTTCCTC
O119	mNc\|e-R(G_Gal4DBD)	gcagcttcatCCGCCGGCGCTTGCGGGA
O120	Gal4DBD-F(G_mNc\|e)	gcgccggcggATGAAGCTGCTGAGCAGCATC
O121	Gal4DBD-R(G_SV40NLS)	tacctcctcctccCACTGTCAGCTGCCGCTG
O122	SV40NLS-F(G_Gal4DBD)	gctgacagtgGGAGGAGGAGGTAGTCCAAAAAAG
O123	mNc\|e-R(G_PIP)	cccttgacatCCGCCGGCGCTTGCGGGA
O124	PIP-F(G_mNc\|e)	gcgccggcggATGTCAAGGGGGGAGGTTAG
O125	PIP-R(G_SV40NLS)	tacctcctcctccGGCCTGCTCCACCATAGC
O126	SV40NLS-F(G_PIP)	ggagcaggccGGAGGAGGAGGTAGTCCAAAAAAG
O127	mNc\|e-R(G_ZF21-16)	tagggctagcCCGCCGGCGCTTGCGGGA
O128	ZF21-16-F(G_mNc\|e)	gcgccggcggGCTAGCCCTAGAGTGAGAACAGG
O129	ZF21-16-R(G_SV40NLS)	tacctcctcctccGGATCCCTTCTCGCCTGTG
O130	SV40NLS-F(G_ZF21-16)	gaagggatccGGAGGAGGAGGTAGTCCAAAAAAG
O131	mNc\|e-R(G_ZF42-10)	tagggctagcCCGCCGGCGCTTGCGGGA
O132	ZF42-10-F(G_mNc\|e)	gcgccggcggGCTAGCCCTAGAGTGAGAACAG
O133	ZF42-10-R(G_SV40NLS)	tacctcctcctccGGATCCCTTTTCTCCGGTG
O134	SV40NLS-F(G_ZF42-10)	aaagggatccGGAGGAGGAGGTAGTCCAAAAAAG
O135	mNc\|e-R(G_ZF43-8)	tggggctagcCCGCCGGCGCTTGCGGGA
O136	ZF43-8-F(G_mNc\|e)	gcgccggcggGCTAGCCCCAGAGTGAGAAC
O137	ZF43-8-R(G_SV40NLS)	tacctcctcctccGGATCCTTTTTCACCTGTATGTG
O138	SV40NLS-F(G_ZF43-8)	aaaaggatccGGAGGAGGAGGTAGTCCAAAAAAG
O139	mNc\|e-R(G_ZF54-8)	tgggagaagcCCGCCGGCGCTTGCGGGA
O140	ZF54-8-F(G_mNc\|e)	gcgccggcggGCTTCTCCCAGAGTAAGAACGGG
O141	ZF54-8-R(G_SV40NLS)	tacctcctcctccGGACCCTTTTTCGCCGGTG
O142	SV40NLS-F(G_ZF54-8)	aaaagggtccGGAGGAGGAGGTAGTCCAAAAAAG
O143	mNc\|e-R(G_ZFHD1)	atgggcgttcCCGCCGGCGCTTGCGGGA
O144	ZFHD1-F(G_mNc\|e)	gcgccggcggGAACGCCCATATGCTTGC
O145	ZFHD1-R(G_SV40NLS)	tacctcctcctccGTTGATTCTTTTTTCTTTCTGGC
O146	SV40NLS-F(G_ZFHD1)	aagaatcaacGGAGGAGGAGGTAGTCCAAAAAAG
O147	EcoRV-mcherry-F(KOD)	GATATCATGGTGAGCAAGGGCGAGGAGG
O148	P2A-F(6-AgeI-EcoRI)	GCAGCAACCGGTGGTGGAGAATTCggAtcAggTgccaccaacttctccctgctgaag
O149	EGFP-R(HindIII-6)	GCAGCAAAGCTTTTActtgtacagctcgtccatgccga
O150	KRAB-R(G_P2A)	AGTTGGTGGCACCTGATCCGAATTCCACCAGCCAGGGTTCCTCGC
O151	KRAB-F(GGGGS-SV40NLS)	GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAatggacgccaagagcctg
O160	lacIF	accccggccccagatccgatATGAAACCAGTAACGTTATAC
O161	LacIR	ggtaccgtcgAACCTTCCTCTTCTTCTTAG
O162	SV40F	gaggaaggttCGACGGTACCGCGGGCCC
O163	sv40R	attaataactaCACCATGGTGGCGACCGGTG
O164	pCAGF	caccatggtgTAGTTATTAATAGTAATCAATTACGGG
O165	pCAGR	tcgcccttgctcaccatgatCTGTAGGAAAAAGAAGAAGG
O166	tTa-R(EcoRI-6)	GCAGCAGAATTCCTACCCACCGTACTCGTCAATTCC
O167	tTa-R(EcoRI-6	f)	GCAGCAGAATTCCCCACCGTACTCGTCAATTCC
O168	EGFP-R(S)	gctgaacttgtggccgtttac
O169	mcherry-R(S)	TGATGATGGCCATGTTATCCTCC
O170	PIR/ZFD F	tttctggaattcgcggccgcttcTAGag
O171	pSBIC3 F	Ggcagaatttcagataaaaaaaatccttagctttcgctaaggatg
O172	pSBIC3 R	gcagggtggtgacaccttgcccttttttg
O173	NPminCMV	GAATTCGCGGCCGCTTCTAgagaggtaggcgtgtacggtg
O174	P2A-R(G_EcoRV-mcherry)	TCGGATCTGGGGCCGGGGTTCTCCTCCACGTCGC
O175	mcherry-F(G_P2A-EcoRV)	ccggccccAGATCCGATATCATGGTGAGCAAG
O176	SV40poly(A)-F	AACTTGTTTATTGCAGCTTATAATGG
O177	SV40poly(A)-R1	TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTG
O178	SV40poly(A)-R2	GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
O179	rtTA-F-(AgeI)	tttctgACCGGTATGTCTAGATTAGATAAAAGTAAAGTGATTAACAGCG
O180	P2A-R(G2_mcherry)	ccatggtggcgatatcGGGGCCGGGGTTCTCCTC
O181	mcherry-F(G2_P2A)	ccggccccgatatcgccaccATGGTGAGCAAGGGCGAGGagg
O182	Kaba_B_R	taaacaagttctaCACCAGCCAGGGTTCCTC
O183	LPA_F	gctggtgtagAACTTGTTTATTGCAGCTTATAATG
O184	LPA_R	catccttagcGATCCAGACATGATAAGATAC
O185	Prom_F	tgtctggatcGCTAAGGATGATTTCTGGAATTCGCGGC
O186	Prom_R	ttgctcaccatggtggcgatTTGCCGGACTGCAGCGGC
O187	TF1_F(same with tf4)	aggagaaccccggccccgatGCTAGCCCTAGAGTGAGAAC
O188	TF2_F	aggagaaccccggccccgatGCTAGCCCCAGAGTGAGAAC
O189	TF3_F	aggagaaccccggccccgatGCTTCTCCCAGAGTAAGAACG
O190	TF8_F	aggagaaccccggccccgatGAACGCCCATATGCTTGC
O191	TF9_F	aggagaaccccggccccgatATGTCAAGGGGGGAGGTTAG
O192	lacI_F	aggagaaccccggccccgatATGAAACCAGTAACGTTATAC
O193	LacI_R	taaacaagttctaAACCTTCCTCTTCTTCTTAG
O194	lacI_40_F	gaggaaggtttagAACTTGTTTATTGCAGCTTATAATG
O195	LacI_40_R	attaataactaGATCCAGACATGATAAGATAC
O196	pCAGop_F	tgtctggatcTAGTTATTAATAGTAATCAATTACGGG
O197	pCAGop_R	ttgctcaccatggtggcgatCTGTAGGAAAAAGAAGAAGG
O198	VP64-F(GGGGS-SV40NLS)	GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAGACGCGCTGGACGatt
O199	VP64-R	TAACATATCGAGATCGAAATCGTCCAGag
O200	VP64-F2(GSSSS-SV40NLS)	GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAgatgctttagacgattttgactt
O201	VP64-R2	tagcatatctagatcaaagtcatctagg
O202	rtTAA-F(6-AgeI)	GCAGCAACCGGTatgtctagactggacaagagcaaag
O203	rtTAA-R(HindIII-6)	TGCTGCAAGCTTttacccggggagcatgtcaag
O204	gal_F	aggagaaccccggccccgatATGAAGCTGCTGAGCAGC
O205	Gal_poly(a)_R	acatgagatcGATCCAGACATGATAAGATAC
O206	Uas_F	tgtctggatcGATCTCATGTGATTACGCC
O207	Uas_R	ttgctcaccatggtggcgatTGTACAAACTTGGGTCGAATTG
O208	PGK-F(6-EcoRI-6-BamHI-6-MfeI)	GCAGCAGAATTCGGTGGAGGATCCGGAGGTCAATTGgggtaggggaggcgc
O209	PuroR-R(NotI-6)	TGCTGCGCGGCCGCtcaggcaccgggcttg
O210	EGFP-R(NotI-6-HindIII6)	TGCTGCAAGCTTTGCAGCGCGGCCGCTTActtgtacagctcgtccatgccgag
O211	rtTAA-F(6-EcoRI)	GCAGCAGAATTCatgtctagactggacaagagcaaag
O212	rtTAA-R(BamHI-6)	TGCAGCGGATCCttacccggggagcatgtcaag
O213	Gal4DBD-F	ATGAAGCTGCTGAGCAGCatc
O214	PIP-F	ATGTCAAGGGGGGAGGTTAG
O215	ZF21-16\|24-10-F	GCTAGCCCTAGAGTGAGAACAGG
O216	ZF43-8-F	GCTAGCCCCAGAGTGAGAAC
O217	ZF54-8	GCTTCTCCCAGAGTAAGAACGG
O218	ZFHD1-F	GAACGCCCATATGCTTGCC
O219	M13fwdL	gtgtaaaacgacggccagtg
O220	M13revL	gctatggacaggaaacagctatgac
O221	G_puro_F	tgacatgctccccgggtaaaGCAGCAGAATTCGGTGGAGGATC
O222	G_puro_R	aagttatattaagggttccaTCAGGCACCGGGCTTGCG
O223	G_pCAGop_R	ttgctcaccatggtggcgatATGAGACAGCACAACAAC
O224	PGK-R(S)	gtgtagcgccaagtgcc
O225	Ne-R(KOD)	CCCTGATTGTAGCAGGGGTTGCTAC
O226	Ne-F(KOD)	CACCTGTGAGCCCACATCCGAGAAC
O227	M13revL2	AATTTCACACAGGAAACAGCTATGAC
O228	EGFP-F(6-MfeI-Kozak)	GCAGCACAATTGGCCACCatggtgagcaagggcgag
O229	mCherry-F(6-Kozak-MfeI)	GCAGCAICAATTGGCCACCATGGTGAGCAAGGGCGAG
O230	PGK-F(6-EcoRI-6-BamHI-6-SalI)	GGAGGTGAATTCGGTGGAGGATCCGGAGGTGTCGACgggtaggggaggcgc
O231	PGK-R(MfeI-6)	GCAGCACAATTGcgaaaggcccggagatgag
O232	mCherry-R(NotI-6-HindIII-6)	GGTGGTAAGCTTGGTGGAGCGGCCGCTTACTTGTACAGCTCGTCCATGCC
O233	Gal4DBD-F(6-MfeI-Kozak)	GCAGCACAATTGGCCACCATGAAGCTGCTGAGCAGCatc
O234	PIP-F(6-MfeI-Kozak)	GCAGCACAATTGGCCACCATGTCAAGGGGGGAGGTTAG
O235	ZF21-16\|42-10-F(6-MfeI-Kozak)	GCAGCACAATTGGCCACCatgGCAAGCCCTAGAGTGAGAACAGgttc
O236	ZF43-8-F(6-MfeI-Kozak)	GCAGCACAATTGGCCACCatgGCAAGCCCCAGAGTGAGAACTGGTTCTAAGACACCTCC
O237	ZF54-8-F(6-MfeI-Kozak)	GCAGCACAATTGGCCACCatgGCTTCTCCCAGAGTAAGAACGG
O238	ZFHD1-F(6-MfeI-Kozak)	GCAGCACAATTGGCCACCATGGAACGCCCATATGCTTGCc
O239	KRAB-R(TAA-HindIII-6)	GCAGCAAAGCTTTTACACCAGCCAGGGTTCCTC
O240	VP64-R(TAA-HindIII-6)	GCAGCAAAGCTTTTAtagcatatctagatcaaagtcatctagggcatc
O241	P2A-R(MfeI-6-HindIII-6)	TCCTCCAAGCTTTCCTTACAATTGGGGGCCGGGGTTCTC
O242	EGFP\|mCherry-F(6-AgeI-6-EcoRI-Kozak)	GGAGGAACCGGTGGAGGTGAATTCGCCACCATGGTGAGCAAGGGCGAGGAG
O243	EGFP\|mCherry-F(6-BamHI-AgeI)	GCAGCAGGATCCACCGGTgccaccatggtgagcaagggcgag
O244	L.Lanier loxp seq	tggtgggtacctagtggaacc
O245	pSB1C3-BS(L)(AgeI-6)	GCAGCAACCGGTgccggactgcagcggccgc
O246	EGFP-F(L_CD8-alpha-SP1)	tgctgctgcccctggccctgctgctgcacgccgccagaccTatggtgagcaagggcgag
O247	EGFP-F(L_CD8-alpha-SP2)	ATGgccctgcccgtgaccgccctgctgctgcccctgg
O248	CD8-alpha-SP-F(6-AgeI)	GCAGCAACCGGTGCCACCATGgccctgcccgt
O249	EGFP-R(OE_CD86tm)	gtgaagataaaagccgcttgtacagctcgtccatgc
O250	CD86tm-F(OE-EGFP)	ggacgagctgtacaagcggcttttatcttcacctttctct
O251	CD86-R(HindIII-6)	GCAGCAAAGCTTTCAatcccgaggccgcttc
O252	CD86tm-F(L)	cggcttttatcttcacctttctctatagagcttgaggaccctcagc
O253	uas-t-R	gagatgatccagacatgataagatacattg
O254	uas-t-F	cttctggatccaattcgacccaag
O255	Sv40-UAS-F	tatcatgtctggatcatctcGTGTGTCAGTTAGGGTGTG
O256	SV40-UAS-R	tggcgtaatcTTTGCAAAAGCCTAGGCC
O257	Gal4BDF	cttttgcaaaGATTACGCCAAGCTACGG
O258	Gal4BDR	ggtcgaattggatccagaagTATCTAGAGTCTCCGCTC
O259	mCherry-F(6-Kozak-MfeI)	GCAGCACAATTGGCCACCATGGTGAGCAAGGGCGAG
O260	UAS-pre	ttctggAATTCGCGGCCGCTTCTAGaggatcatctcgtgtgtcagtta
O261	UAS-suf	gccggacTGCAGCGGCCGCTACTAGTAagaaggtaactagagtctccgctcg
O262	LaG16-F(L_CD8-alpha-SP1)	CGCCAGACCTGAGCAAAAGTTGATCTCAGAGGAGGACCTCATGACCATGGCCCAGGTG
O263	P2A-R(L_CD8-alpha-SP1)	GCAGGGCCAGGGGCAGCAGCAGGGCGGTCACGGGCAGGGCCATGGGGCCGGGGTTCTC
O264	P2A-R(L_CD8-alpha-SP2)	CTCCTCTGAGATCAACTTTTGCTCAGGTCTGGCGGCGTGCAGCAGCAGGGCCAGGGGC
O265	P2A-R(L_CD8-alpha-SP2-HA)	GTAATCTGGCACGTCATAGGGGTAAGGTCTGGCGGCGTGCAGCAGCAGGGCCAGGGGC
O266	alpha-GPC3-F(L_CD8-alpha-SP1)	CTTACCCCTATGACGTGCCAGATTACGCGGATGTTGTGATGACTCAGTCTCCACTCTC
O267	LaG16-F(L_CD8-alpha-SP2)	GACCGCCCTGCTGCTGCCCCTGGCCCTGCTGCTGCACGCCGCCAGACCTGAGCAAAAG
O268	CD8-alpha-SP-F(6-AgeI-Kozak)	GCAGCAACCGGTGCCACCATGGCCCTGCCCGTGACCGCCCTGCTGCTGCCCC
O269	VP64-R(EcoRI-6	f)	GCAGCAGAATTCtagcatatccagatcaaagtcatctagggcatcgg
O270	pUAS-F(6-BP)	GCAGCAGAATTCGCGGCCGCTTCTAGAGcatgtgattacgccaagctacggg
O271	pUAS-R(BS-6)	GCAGCACTGCAGCGGCCGCTACTAGTActagagtctccgctcggaggacaga
O272	P2A-F(S_6-BP)	GCAGCAGAATTCGCGGCCGCTTCTAGAGACCAACTTCTCCCTGCTGAAG
O273	pML2-R(S_BS-6)	GCAGCACTGCAGCGGCCGCTACTAGTAacgagattccagcaggtcg
O274	pCMV-F(S_6-BP)	GCAGCAGAATTCGCGGCCGCTTCTAGAGcgtgtacggtgggaggtctatataag
O275	mTagBFP2-F(4-EcoRV-Kozak)	CGGccccGATATCGCCAccATGGTGTCTAAGGGCGAAGAGCTGATTAAGGAGAACATGC
O276	mTagBFP2-F(L1-T2A)	GGGCAGGGGAAGTCTTCTAACtTGCGGGGACGTGGAGGAAAATCCCGGCCCCGATATCG
O277	mTagBFP2-F(L2-T2A)	GCAGCAaCCGGTGGTGAATTCGGACAATTGggAtcAggTGAGGGCAGGGGAAGTC
O278	mTagBFP2-R(HindIII-6)	GCAGCAAAGCTTTTAATTAAGtTTGTGCCCCAGTTTGCTAGG
O279	EBFP2-F(4-EcoRV-Kozak)	CGGCCCCGATATCGCCAccatggtgagcaagggcg
O280	EBFP2-R(HindIII-6)	GCAGCAAAGCTTttacttgtacagctcgtccatg
O281	mTagBFP2-F(L2-T2A)	GCAGCAGAATTCGGTaCCGGTGGACAATTGggAtcAggTGAGGGCAGGGGAAGTC
O282	PIP-F(6-AgeI)	GCAGCAACCGGTGCCACCATGTCAAGGGGGGAGGTTAG
O283	ZF43-8-F(6-AgeI)	GCAGCAACCGGTGCAAGCCCCAGAGTGAGAAC
O284	PIP-F(6-AgeI-Kozak)	GCAGCAACCGGTGCCACCatggtgTCAAGGGGGGAGGTTAGAATGG
O285	ZF43-8-F(6-AgeI-Kozak)	GCAGCAACCGGTGCCACCatgGCAAGCCCCAGAGTG
O286	P2A-F(6-SbfI-C)	GCAGCACCTGCAGGCggctccggcgccac
O287	KRAB-R(MfeI-6)	GCAGCACAATTGcaccagccagggttcctc
O288	mTagBFP2-NR(S)	GCACTTGAAGTGATGGTTGTCC
O289	P2A-F(3-AgeI-Kozak-ATG-EcoRI-BamHI-SalI)	GCAACCGGTGAATTCGCCACCATGGGATCCGTCGACGGATCAGGTGCCACCAAC
O290	mCherry-R(CAAX-HindIII-1)	TAAGCTTtcaggagagcacacacttgcagctcatgcagccCTTGTACAGCTCGTCCATG
O291	mCherry-F(LifeAct-L1)	AGTCCATCTCCAAGGAGGAGGGCGATCCCCCGGTCGCCACCATGGTGAGCAAGGGCGAG
O292	mCherry-F(LifeAct-L2)	ATGGGCGTGGCCGACTTGATCAAGAAGTTCGAGTCCATCTCCAAGGAGGAGG
O293	LifeAct-F(3-EcoRI-BamHI-Kozak)	GCAGAATTCGGATCCGCCACCATGGGCGTGGCCGAC
O294	mCherry-R(P2A-L1)	CGCcggcctgcttcagcagggagaagttggtggcgccggagccCTTGTACAGCTCGTCC
O295	mCherry-R(P2A-L2)	TAAGCTTGTCGACCTACAATTGGGGGCCGGGGTTCTCctccacgtcgccggcctgcttc
O296	Hist2h2bb-F(3-EcoRI-BamHI-Kozak)	GGTGAATTCGGATCCGCCACCatgccggacccagcgaag
O297	Hist2h2bb-R(M)	cgttcacgaaggaGttcatgattcccatagccttagatgagatg
O298	Hist2h2bb-F(M)	catgaaCtccttcgtgaacgaCatcttcgagcgcatcgc
O299	Hist2h2bb-R(OE_mCherry)	CATTgtGgcCacAggAggGtccttggagctggtgtacttggtg
O300	mCherry-F(OE_Hist2h2bb)	acccTccTgtGgcCacAATGGTGAGCAAGGGCGAG
O301	mCherry-R(OE_mito)	cgcctctcccgttCTTGTACAGCTCGTCCATGCC
O302	mito-F(OE_mCherry)	GTACAAGaacgggagaggcggtagac
O303	mito-R(HindIII-3)	GCAAAGCTTttaattattttttcttaattgaataattttgataaacgcccc
O304	Myc-F	GAGCAGAAATTGATCTCTGAAGAGGAC
O305	pML2-C0-R	gcatacattatacgaagttatattaagggttccaag
O306	mNce-F	CCCTGCGTCGGTAGCAAC
O307	tTAA-F(3-MfeI)	GCACAATTGATGAGTCGATTGGATAAATCTAAGGTAATTAACAG
O308	tTAA-R(HindIII-3)	GCAAAGCTTTCATCCTGGGAGCATATCCAGG
O309	TetOn3G-F(3-MfeI)	GCACAATTGATGAGCCGACTGGACAAGAG
O310	TetOn3G-R(HindIII-R)	GCAAAGCTTTTAGCCCGGCAACATATCAAGATC
O311	pSV40-F(3-BP)	GCAGAATTCGCGGCCGCTTCTAGAGgtgtgtcagttagggtgtgg
O312	pSV40-R(BS-AgeI-3)	GCAACCGGTctgcagcggccgcTACTAGTAtttgcaaaagcctaggcctcc
O313	pTight-F(3-BP)	GCAGAATTCGCGGCCGCTTCTAGAGGCCGGAATTGCTCGAGTTTAC
O314	pTight-R(BS-AgeI-3)	GCAACCGGTctgcagcggccgctactagtaGGCGATCTGACGGTTCACTAAAC
O315	TRE3GV-F(3-BP)	GCAgaattcgcggccgcttcTAGAGCCCTTTCGTCTTCACTCGAGTTTAC
O316	TRE3GV-R(BS-AgeI-2)	CAACCGGTctgcagcggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc
O317	minCMV-F(3-BP)	GCAgAATTCGCGGCCGCTTCTAGAGGGTAGGCGTGTACGGTGG
O318	minCMV2-F(3-BP)	GCAgaattcgcggccgcttcTAGAGGGTAGGCGTGTACGGTGG
O319	pSB1C3-VF(S)	TGCCACCTGACGTCTAAGAA
O320	pSB1C3-VR(S)	ATTACCGCCTTTGAGTGAGC
O321	prefix-mNotch(e)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGCCCTGCGTCGGTAGCA
O322	mNotch(e)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTACCGTCGGCGCTTTCG
O323	prefix-hNotch(e)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGCCCTGTCTTGGAGGGAATCC
O324	hNotch(e)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTACCGGCGTCGTTTTCGAC
O325	pSB1C3-F	GCAGCATACTAGTAGCGGCCGCTG
O326	pSB1C3-R	GCAGCACTCTAGAAGCGGCCGCG
O327	prefix-LaG17(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGTGAACCGTCAGATCAGATCCGCTAG
O328	LaG17(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGCA
O329	prefix-CAR3G(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGCCAGCCAAACCAACAACTACAC
O330	CAR3G(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccAAGCT
O331	prefix-EGFPtm(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGTGGAAGAAAATCCCGGTCCG
O332	EGFPtm(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagct
O333	prefix-tTAA(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGTGTCACGAAAGCGCCG
O334	tTAA(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagct
O335	IL-2(CO)-F(G_pSB1C3)	GAAGATCTCACGACGtgaaccgtcagatccgctag
O336	IL-2(CO)-R(G_pSB1C3)	GATGATCATACGACGATACGAAGTTATATTAAGGGTTCCAAGC
O337	IL-10(CO)-F(G_pSB1C3)	GAAGATCTCACGACGtgaaccgtcagatccgctag
O338	IL-10(CO)-R(G_pSB1C3)	GATGATCATACGACGATACGAAGTTATATTAAGGGTTCCAAGC
O339	LaG16-2(CO)-F(G_pSB1C3)	GAAGATCTCACGACGtgaaccgtcagatccgctag
O340	LaG16-2(CO)-R(G_pSB1C3)	GATGATCATACGACGGGGTTGCTACCGACGC
O341	prefix-CD47tm(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc
O342	CD47tm(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTACGGACCGGGATTTTCTTCC
O343	prefix-hYP9	1scFv(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc
O344	hYP9	1scFv(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAACCTGGGACCCCCC
O345	prefix-LaG 16-2(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC
O346	LaG 16-2(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGC
O347	prefix-OKT3scFv(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGGGTGGCGGGGGGTC
O348	OKT3scFv(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagcTTC
O349	prefix-Tet-On3G(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGGGAGGTgaattcGCCACC
O350	Tet-On3G(CO)-R -suffix	GCAGCACTGCAGCGGCCGCTACTAGTAACCTCCggatccTTAGCC
O351	prefix-α-CD19(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC
O352	α-CD19(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGCAG
O353	prefix-α-CD47(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC
O354	α-CD47(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAGTAGTTGTTGGTTTGGCTGGC
O355	prefix-IL-2(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc
O356	IL-2(CO)-R-suffix	GCAGCACTGCAGCGGCCGCTACTAGTAATACGAAGTTATATTAAGGGTTCCAAGC
O357	IL-10(CO)-F	GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC
O358	IL-10(CO)-R	GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagctTC
O359	pML2-C0(AgeI)	tgaaccgtcagatccgctagc
O360	pML2-C0(HindIII)	atacgaagttatattaagggttccaagc
O361	mNc-F	ATACTCGACTACAGTTTTACTGGGG
O362	LaG2-R(OE_mNc)	CCCCAGTAAAACTGTAGTCGAGTATAACAGTGACTTGCGTTCCCTG
O363	LaG17-R(OE_mNc)	CCCCAGTAAAACTGTAGTCGAGTATGGAAACGGTCACCTGTGTACC
O364	CD8SP-F(3-AgeI-Kozak)	GCAACCGGTGCCACCATGGCCCTTCCTGTAACGG
O365	PDGFRB-R(TGA-HindIII-3)	GCAAAGCTTTCATCTAGGTTTCTTCTGCCAAAGCATGATC
O382	2*ZF21-16-F(Scar)	tactagattagaagtgaattagaagtga
O383	2*ZF21-16-R(Scar)	ctagtatcacttctaattcacttctaat
O384	BP-BbsI-F(3)	ctggaattcgcggccgcttctagaGAAGAC
O385	3-BP-BbsI-R(Scar)	ctagtaGTCTTCtctagaagcggccgcgaattccag
O386	BS-3-F	TACTAGTAGCGGCCGCTGCAGtcc
O387	BS-R(3)	ggaCTGCAGCGGCCGCTA
O388	EGFP-F(myr-L1)	ccaaggatgccagccagcggcgccgcGGTGGTGGAGGCTCAATGGTGAGCAAGGGCGAG
O389	O389 EGFP-F(myr-L2)	TGAATTCGGATCCGCCACCatgggtagcaacaagagcaagcccaaggatgccagccagc
O390	2*ZF21-16-A-F(A)	tactagattagaagtgaattagaagtgatactag
O391	2*ZF21-16-A-R(A)	tcacttctaattcacttctaat
O392	2*ZF21-16-B-F(A)	attagaagtgaattagaagtga
O393	2*ZF21-16-B-R(A)	ctagtatcacttctaattcacttctaatctagta
O394	BP-F(A)	GATCCGaattcgcggccgcttctagaGAAGAC
O395	BP-R(A)	ctagtaGTCTTCtctagaagcggccgcgaattcg
O396	BS-F(A)	TAGCGGCCGCTGCAGA
O397	BS-R(A)	AGCTTCTGCAGCGGCCGCTActagta
O398	BP-F	Cgaattcgcggccgc
O399	BS-R	TCTGCAGCGGCCGC
O400	pSB1A3-C(3-BP-BamHI)	GCActctagaagcggccgcgaattcGGATCCgaaatcatccttagcgaaagctaagg
O401	pSB1A3-C(3-BS-HindIII	C)	GCAtactagtagcggccgctgcagAAGCTTtccggcaaaaaagggcaagg
O404	mNCe-F(M)	CCTTGTGTTGGtAGTAATCCATGCTACAATCAAGGGACCTGCG
O405	mNCe-F	CCTTGTGTTGGtAGTAATCCATGCTAC
O406	mNC\|e-R(M)	cctTCTtcttTTTCGTGACAGCAAAACACCGCAGCCTAC
O407	mNC\|e-R	cctTCTtcttTTTCGTGACAGCAAAAC
O408	LaG2-R(G_mNCe)	ggattactaccaacacaaggAACAGTGACTTGCGTTCCCTGC
O409	LaG17-R(G_mNCe)	ggattactaccaacacaaggGGAAACGGTCACCTGTGTACC
O410	tTAA-F(G_mNCe)	TGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAG
O411	pML2-CX-R(Lin)	CACCGGTaActcaagATgatctgacTAttcactacagctctgcttatatagacctccc
O412	pML2-CX-F(Lin)	TCAATTGGGAACTCGATACCTTCTAATATCTCATAataacttcgtataatgtatgctatacgaagttattaggtccctcgacctgctg
O413	MCS-F(A)	TGAATTCGGATCCAGGAGCAGTCTTCGGGCCCGAAGACTGCCATAGTCGACAAGCTTC
O414	MCS-R(A)	GAAGCTTGTCGACTATGGCAGTCTTCGGGCCCGAAGACTGCTCCTGGATCCGAATTCA
O415	pCMV-F2(S)	cccattgacgcaaatgggc
O416	CD8aSP-F(Kozak	G_pML2-CX)	gtgaaTAgtcagatcATcttgagTtACCGGTGAATTCGCCACCATGGCGCTGCCGG
O417	LaG2-R(G_mNCe)	ATTaTAGCAaGGATTACTACCAACACAAGGAACAGTGACTTGCGTTCCCTGC
O418	mNCe-F(M)	CCTTGTGTTGGTAGTAATCCtTGCTAtAATCAAGGGACCTGCGAGC
O419	mNC-F(M)	ATACTCGACTAtAGTTTTACaGGGGGGGCGGGCCGGGATATACCCCCG
O420	mNC\|e-R(M)	CCTTCTTCTTTTTCGTGACAGCAAAACtCCGCAGCCTACGAAGAACAAGAGTAC
O421	tTAA-F(M)	GGaGTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGG
O422	tTAA-R(G_pML2-CX)	GAGATATTAGAAGGTATCGAGTTCCCAATTGAAGCTTTCATCCTGGGAGCATATCCAGG
O423	pML2-R(Lin	L1)	tcaagATgatctgacTAttcactacagctctgcttatatagacctcccaccgtacacgc
O424	pML2-R(Lin	L2)	CGAATTCACCGGTaActcaagATgatctgacTAttcactacagctc
O425	pML2-F(Lin	L2)	CAAGCTTCAATTGGGAACTCGATACCTTCTAATATCTC
O426	pML2-CX(EcoRI)	gtagtgaaTAgtcagatcATcttgagTtACCGGTG
O427	pML2-CX(HindIII)	TATGAGATATTAGAAGGTATCGAGTTCCCAATTGAAGC
O428	pML2X-R(Lin1)	AAGACCACCGGTGAATTCattaagggttccggttccactaggtacccacc
O429	pML2X-R(Lin2)	CGTCGACTAGCCCTAACGTGATCTGACAGTACATTAAGACCACCGGTGAATTCattaag
O430	pML2X-F(Lin1)	TGAtaggtccctcgacctgctggaatctcgtg
O431	pML2X-F(Lin2)	GAAGCTTCCCTCATTCAAGTACCAGTCTAGTCGAAGTGTGAtaggtccctcgacctgc
O432	MCS-F(A)	AGTCGACGGATCCAGGAGCAGTCTTCCTGCAGGAAGACTGCCATACAATTGAAGCTTC
O433	MCS-R(A)	GAAGCTTCAATTGTATGGCAGTCTTCCTGCAGGAAGACTGCTCCTGGATCCGTCGACT
O434	pML2X-F(S)	atcctccctttatccagccc
O435	pML2X-R(S)	atcgataagctcgcttcacg
O436	pML2Y-F(Lin1)	CGGCCGCtaggtccctcgacctgctggaatctcgtg
O437	pML2Y-F(Lin2)	GAAGCTTCCCTCATTCAAGTACCAGTCTAGTCGAAGTGTGAGCGGCCGCtaggtccctc
O438	pML2Y(G_SalI)	GGTCTTAATGTACTGTCAGATCACGTTAGGGC
O439	pML2Y(G_HindIII)	TCACACTTCGACTAGACTGGTACTTGAATGAGG
O440	pML2X-R(Lin2)	CGTCGACTAGCCCTAACGTGATCTGACAGTACAaTAAGACCACCGGTGAATTCattaag
O441	pML2Y(G_SalI)	GGTCTTAtTGTACTGTCAGATCACGTTAGGGC
O442	CMV-F(3-EcoRI)	GCAGAATTCcgttacataacttacggtaaatggcccgcctggctg
O443	pCMV-R(AgeI-3)	GCAACCGGTagctctgcttatatagacctcccaccgtacacgcc
O444	PGK-F(3-EcoRI)	GCAGAATTCgggtaggggaggc
O445	PGK-R(AgeI-G_pML2Y-SalI)	CGTCGACTAGCCCTAACGTGATCTGACAGTACAATAAGACCACCGGTcgaaaggcccgg
O446	CD8aSP-F(G_pML2Y)	GGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTGCCG
O447	P2A-R(G_CD8aSP)	tacaggaagggccatCGGACCGGGATTTTCTTCCACGTC
O448	CD8aSP-F(G_P2A)	gaaaatcccggtccgATGGCCCTTCCTGTAACG
O449	PDGFRBtr-R(G_pML2Y)	TCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTTCAGCGAGGTTTCTTTTGC
O450	CD8aSP-F(G_pML2Y)	GGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCG
O451	tTAA-R(G_pML2Y)	TCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTTCATCCTGGGAGCATATC
O452	LaG17-R(G_mNCe)	attatagcaaggattactaccaacacaaggGGAAACGGTCACCTGTGTACCTTGTCC
O453	aCD19-R(G_mNCe)	attatagcaaggattactaccaacacaaggTGATGAAACGGTTACGGATGTCCCTTGTC
O454	LaG16-2-R(G_mNC)	CGCCCCCCCTGTAAAACTATAGTCGAGTATAACAGTGACTTGCGTTCCCTGCC
O455	LaG17-R(G_mNC)	cgccccccctgtaaaactatagtcgagtatGGAAACGGTCACCTGTGTACCTTGTCC
O456	aCD19-R(G_mNC)	CGCCCCCCCTGTAAAACTATAGTCGAGTATTGATGAAACGGTTACGGATGTCCCTTGTC
O457	CD19ECD-R(G_Myc)	gatcaatttctgctcCTTCCATCCCCCCGTGCG
O458	Myc-F(G_CD19ECD)	acggggggatggaagGAGCAGAAATTGATCTCTGAAGAG
O459	PGK-F(S)	cggcattctgcacgcttc
O460	LaG16-F(L1)	acgccgccaggccgGAGCAGAAGCTGATCTCAGAGGAGGACCTGATGGCCCAGGTGCAG
O461	LaG16-F(L2)	ggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggc
O462	LaG16-F(L3)	tgaaccgtcagatccgctagcgctaCCGGTatggccttaccagtgaccg
O463	mNC\|e-R(M)	CCTTCTTCTTTTTCGTGACAGCAAAACTCCACAGCCCACAAAGAACAGG
O464	pML2Y-R(C)	ACCACCGGTGAATTCattaagggttccggttccactaggtacccaccaaattccggcgc
O465	pML2Y-F(C)	TGAGCGGCCGCtaggtccctcgacctgctggaatctcgtgaagcgagcttatcg
O466	XXX-F(1-AgeI-pML2Y-SalI)	CACCGGTGGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACccggggtggtgc
O467	XXX-R(HindIII-pML2Y-NotI-1)	aGCGGCCGCTCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTcccggcggcg
O468	ZF 21-16	GCAGAATTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaTTAGAAGTGaTACTAGTAGCGGCCGCTGCAG
O469	EGFP-F(3-SalI-Kozak-ATG-BamHI)	CTAGTCGACGCCACCATGGGATCCGTGAGCAAGGGCGAGGAG
O470	EGFP/mCherry-R(L1)	GCTTGCTTTAACAGAGAGAAGTTCGTGGCgccggagccCTTGTACAGCTCGTCCATGCC
O471	EGFP-R(L2)	TCAATTGAGGTCCCGGGTTTTCTTCCACGTCTCCTGCTTGCTTTAACAGAGAGAAGTTC
O472	P2A-R(MfeI-BbsI-TGA-SbfI-BbsI-HindIII-3)	GGAAGCTTAGTCTTCCTGCAGGTCATGGAAGACTCAATTGAGGTCCCGGGTTTTCTTCC
O473	CD8aSP-F(3-SalI-Kozak)	GCAGTCGACGCCACCatggccttaccagtgaccg
O474	TRE3GV-F(3-EcoRI)	GCAGAATTCGAGTTTACTCCCTATCagtgatagagaacgtatgaag
O475	TRE3GV-R(AgeI-3)	GCAACCGGTAAGTATAAGACAAAAGTGTTGTGGAATTGcTCC
O476	mCherry-R(KOD)	CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCAC
O477	mCherry-F(KOD)	CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCC
O478	BP-F(M_3-EcoRI)	GCAGAATTCcggccgcttcTAGAG
O479	BS-R(M_1-PstI-AgeI-3)	GCAACCGGTCTGCAGacggccgcTACTAGT
O480	ZF 43-8	GCAGAATTCGCGGCCGCTTCTAGaGAGTGAGGAcaGAGTGAGGAcTACTAGTAGCGGCCGCTGCAG
O481	HIST2H2BE-F(CE_pML2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGcctgaaccggcaaaatcc
O482	HIST2H2BE-R(CE_EGFP)	tccttggagctggtgtacttggtgac
O483	EGFP-F(CE_HIST2H2BE)	aagtacaccagctccaaggaCccTccTgtGgcCacAATGGTGAGCAAGGGCGAG
O484	P2A-R(CE_pML2Y)	GTACTTGAATGAGGGAAGCTTAGTCTTCCTGCAGGTC
O485	LifeAct-F(3-SalI)	CTAGTCGACGCCACCATGGGCGTGGCCGAC
O486	myr-F(3-SalI)	CTAGTCGACGCCACCATGggtagcaacaagagcaagcccaaggatgccagccagc
O487	1-BP-21-16-43-8-SpePI-1-F(A)	CGAATTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTG
O488	1-BP-21-16-43-8-SpeI-1-R(A)	CACTAGTAgTCCTCACTCttCACTTCTAAtCtCTAGAAGCGGCCGCGAATTCG
O489	1-XbaI-21-16-43-8-SpeI-BS-1-F(A)	GTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTAGCGGCCGCTGCAGC
O490	1-XbaI-21-16-43-8-SpeI-BS-1-R(A)	GCTGCAGCGGCCGCTACTAGTAgTCCTCACTCttCACTTCTAAtCtCTAGAC
O491	p2Y(CE_EcoRI)	accggaacccttaatGAATTC
O492	p2Y(CE_AgeI)	CAGTACAATAAGACCACCGGT
O493	p2Y(CE_SalI)	ATCACGTTAGGGCTAGTCGAC
O494	p2Y(CE_HindIII)	GTACTTGAATGAGGGAAGCTT
O495	mNC-F(M)	ATTCTCGACTACAGTTTCACAGGAGGAGCGGGCCGGGATATACCCCCG
O496	tTAA-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCATCCTGGGAGCATATCCAGGTC
O497	CD8aSP-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGG
O498	LaG2-R(CE_mNC)	GTGAAACTGTAGTCGAGAATAACAGTGACTTGCGTTCCC
O499	LaG17-R(CE_mNC)	GTGAAACTGTAGTCGAGAATGGAAACGGTCACCTGTGTACC
O500	aCD19-R(CD_mNC)	GTGAAACTGTAGTCGAGAATTGATGAAACGGTTACGGATGTCC
O501	PGK-F(CE_p2Y)	accggaacccttaatGAATTCgggtaggggaggcgc
O502	PGK-R(CE_p2Y)	CAGTACAATAAGACCACCGGTcgaaaggcccggagatgag
O503	CD8aSP-F2(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTGCCGGTC
O504	CD19ECD(CE_Myc)	atttctgctcCTTCCATCCCCCCGTGC
O505	Myf-F(CE_CD19ECD)	gggatggaagGAGCAGAAATTGATCTCTGAAGAGGAC
O506	PDGFRBtr-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCATCTAGGTTTCTTCTGCCAAAGCATG
O507	TetOn3G-F(CE_P2A)	TGGAAGAAAACCCGGGACCTATGAGCCGACTGGACAAGAGC
O508	TetOn3G-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTTACAATTGGCCCGGCAACATATCAAGATCAAAGTC
O509	U105 pEGFP-C5	CATGGTCCTGCTGGAGTTCGT
O510	mCherry-R(KOD)	CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCTTGCAGGGAGGAGTCCTGGGTCAC
O511	mCherry-F(KOD)	CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAAAAGACCATGGGCTGGGAGGCC
O512	BP-F(M	CE_p2Y-EcoRI)	accggaacccttaatGAATTCcggccgcttcTAGAG
O513	BS(M	CE_p2Y-AgeI)	CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT
O514	BS-R(M	CE_p2Y-AS)	CCCTAACGTGATCTGACAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT
O515	PIP-F(CE_p2Y-AS-Kozak)	ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGTCAAGGGGGGAGGTTAG
O516	KRAB-R(CE_HindIII)	GTACTTGAATGAGGGAAGCTTTTACACCAGCCAGGGTTCC
O517	pSV40-F(CE_p2Y-EcoRI)	accggaacccttaatGAATTCcggccgcttcTAGAGgtgtgtcagttagggtgtgg
O518	ZF21-16-F(CE_p2Y-EcoRI)	accggaacccttaatGAATTCcggccgcttcTAGAGaTTAGAAGTG
O519	CE_P2A-R	AGGTCCCGGGTTTTCTTCC
O520	TetOn3G-F(CE_P2A-MfeI)	TGGAAGAAAACCCGGGACCTCAATTGATGAGCCGACTGGACAAGAGC
O521	TetOn3G-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTTAGCCCGGCAACATATCAAGATCAAAGTC
O522	TRE3GV-F(1-BP-M_p2Y)	CGAATTCcggccgcttcTAGAGGAGTTTACTCCCTATCagtgatagagaacgtatgaag
O523	TRE3GV-R(BS-M_p2Y-AgeI-1)	GACCGGTCTGCAGacggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc
O524	ZF43-8-F(CE_p2Y	M)	accggaacccttaatGAATTCcggccgcttcTAGAGaGAGTGAGG
O525	LaG16-F(CE-P2Y_SalI)	ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGGTCaccgccttgctcctgc
O526	ZF43-8-F(CE_P2A)	TGGAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC
O527	ZF43-8-R	GGAGCCCTTCTCACCTGTATGTGTTTTCAGATGtctgttc
O528	G4S-SV40NLS-F(CE_SynTF-C)	ATACAGGTGAGAAGGGCTCCGGAGGAGGAGGTAGTCCAAAAAAG
O529	KRAB-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTCACAATTGCACCAGCCAAGGTTCTTCTCccttttcc
O530	PIP-F(CE_P2A)	TGGAAGAAAACCCGGGACCTATGTCAAGGGGGGAGGTTAG
O531	ZF43-8-F(CE_p2Y-SalI)	ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGAG
O532	HA-F(CE_pGEX)	TTCCAGGGGCCCCTGGGATCCTACCCCTATGATGTACCAGATTACGC
O533	Myc-R(CE_pGEX)	CTCGAGTCGACCCGGGAATTCTCACAGGTCCTCTTCAGAGATCAATTTC
O534	TetOn3G-F(CE_P2A-MfeI)	GAAAACCCGGGACCTCAATTGATGAGCCGACTGGACAAGAGCAAGGTAATC
O535	TetOn3G-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTTAGCCCGGCAACATATCAAGATCAAAGTCGTCG
O536	CD8aSP-F(CE_P2A)	GTCCGATGGCCCTTCCTGTAACGG
O537	PDGFRBtr(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTCAGCGAGGTTTCTTTTGCCAGAG
O538	T2A-F(CE_p2Y-SalI-BamHI)	ATCACGTTAGGGCTAGTCGACGCAGGATCCggAtcAggTGAAGGTAGGGGGTCATTGC
O539	hYP9.1bscFv-R(CE_OKT3scFV)	GACTGTTGGAGTTGAACCTGACTACCTCCTCCACCCTTGATCTCAAGTTTGGTTCCTTG
O540	OKT3scFv-F	CAGGTTCAACTCCAACAGTCTGG
O541	OKT3scFv-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTCTAGCGGTTTATTTCAAGTTTTGTGCC
O542	2.3D11scFv-R(CE_CAR3G)	GTAGTTGTTGGTTTGGCTGGCTTTATTTCGACTTTCGTGCCTCC
O543	CAR3G-F	CCAGCCAAACCAACAACTACAC
O544	CAR3G-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTCACCGTGGTGGCAAAGCC
O545	IL-10-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTCAGTTCCTTATTTTCATCGTCATATACGCTTC
O546	LaG17-R(CE_hNC)	GAAACTGTAGTCGAGAATGGAAACGGTCACCTGTGTACCTTGTCCC
O547	hNC-F(M)	ATTCTCGACTACAGTTTCGGTGGGGGAGCGGG
O548	hNC\|e-R(M)	CCTTCTTCTTTTTCGTGACAGCAAAACTCCACACCCTACG
O549	mTagBFP2-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGGTGTCTAAGGGCGAAGAG
O550	mTagBFP2-R(M1)	CGTCCTCGTATGTGGTGACTCTCTCCCATGTGAAGCCC
O551	mTagBFP2-F(M2)	AGTCACCACATACGAGGACGGGGGCGTGCTGACCG
O552	mTagBFP2-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCAATTCAGCTTGTGCCCCAGTTTG
O553	mNc F(CE/G_LNR-A [-])	ATTCTCGACTACAGTTTCACAGGAGGAGCGTCACTTAACTTCAATGATCCGTGGAAG
O554	mNc F(CE/G_LNR-ABLnkr[-])	ATTCTCGACTACAGTTTCACAGGAGGAGCGTGCACCCAGTCCCTCCA
O555	mNc F(CE_LNR-A[-]hlx3+)	ATTCTCGACTACAGTTTCACAATCACGCCTGCGGTT
O556	mNC-R(M_S2)	GTTCGCTTTTgtcctCTTCGATTTTATACGGAATATTCAAGCTCCCAAGAGAGGCC
O557	mNC-F(M_S2)	CGAAGaggacAAAAGCGAACCTGTCGAACCGCC
O558	mNC-R(M_S3)	GCAAAAagaagagcagTACGAAGAACAAGAGTACGAACGCAGCAGC
O559	mNC-F(M_S3)	CGTActgctcttctTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGG
O560	MycoP-F	CACCATCTGTCACTCTGTTAA
O561	MycoP-R	GGAGCAAACAGGATTAGATAC
O562	BP-ZF21-16-43-8-BS	TTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTAGCGGCCGCTG
O563	CE ZF21-16-43-8 F	TAAGGATGATTTCTGGAATTCGCGGCCGCTTc
O564	CE ZF21-16-43-8 R	ttgcccttttttgccggaCTGCAGCGGCCGctac
O565	mNC-R-N(S)	CCTCTGTCAGTTGGCAGTC
O566	pGEX usual F(S)	GAGCTGCATGTGTCAGAGG
O567	pGEX usual R(S)	GCAAGCCACGTTTGGTG
O568	d2EGFP-R(L1)	CCTGCTCCTCCACCTCCGGCGGGAAGCCATGGCTCAACTTCTTGTACAGCTCGTCCATG
O569	d2EGFP-R(L2)	ATCCCGCTCTCCTGGGCACAAGACATGGGCAGCGTGCCATCATCCTGCTCCTCCACCTC
O570	d2EGFP-R(L3)	CACATTGATCCTAGCAGAAGCACAGGCTGCTGGGTGACGGTCCATCCCGCTCTCCTGG
O571	d2EGFP-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTCTACACATTGATCCTAGCAGAAGC
O572	CD8aSP-F(CE_p2Y	G3)	ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG
O573	EGFP-R(CE_mN1ce)	GGATTACTACCAACACAAGGCTTGTACAGCTCGTCCATGCC
O574	mN1c-R(C)	CCTTCTTCTTTTTCGTGACAGCAAAACTCCGC
O575	p2Y-F(C_HindIII)	AAGCTTCCCTCATTCAAGTACCAGTCTAGTCG
O576	VP64-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTCAGagcatGtcCagGtcGaagtcatctagggcatcgg
O577	Gal4-F(CE_m\|hN1c)	TCACGAAAAAGAAGAAGGATGAAGCTGCTGAGCAGCatc
O578	ZF21-16-F(CE_m\|hN1c)	TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC
O579	ZF42-10-F(CE_mN1c)	TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCAC
O580	ZF43-8-F(CE_mN1c)	TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC
O581	KRAB-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTCACACCAGCCAAGGTTCTTCGCccttttccagcc
O582	rtTAA-F(CE_m\|hN1c\|e)	TCACGAAAAAGAAGAAGGatgtctagactggacaagagc
O583	rtTAA-R(CE_p2Y-HindIII)	GTACTTGAATGAGGGAAGCTTTCAcccggggagcatgtcaag
O584	mN1c-F-C(S)	CCAGTTGCACCTCATGTACG
O585	mTagBFP2-R(L1)	CCTGCTCCTCCACCTCCGGCGGGAAGCCATGGCTCAACTTATTCAGCTTGTGCCCCAG
O586	rtTAA-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCatgtctagactggacaagagcaaag
O587	rtTAA-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTttacccggggagcatgtcaag
O588	VP64-R(MfeI	CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCACAATTGtagcatatcCagatcaaagtcatctaggg
O589	ZF21-16-F(CE_P2A)	GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC
O590	ZF42-10-F(M	CE_p2Y)	accggaacccttaatGAATTCcggccgcttcTAGAGaGACGCTG
O591	CMV-F(BP_M)	tGAATTCcggccgcttcTAGAGcgttacataacttacggtaaatg
O592	CMV-R(BS_M)	cACCGGTCTGCAGacggccgcTACTAGTAagctctgcttatatagacctc
O593	TetO-R(BS_M)	cACCGGTCTGCAGacggccgcTACTAGTATCTCTATCACTGATAGGGAgtaaactggat
O594	EGFP-F(L1_palm)	CaaaatgcggctgctgcttcgcccgggaCccaccCgtcgccaccATGGTGAGCAAGGGC
O595	EGFP-F(L2_palm)	Gggggtcgctgtgcctgtccccgtgtgatccgaaaagtgctggcaaaatgcggctgctg
O596	palm-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCatgcggggggggcacaaagggggtcgctgtgc
O597	mCherry-F(CE_mN1c-SV40NLS)	TCACGAAAAAGAAGAAGGCCAAAAAAGAAGAGAAAGGTAATGGTGAGCAAGGGCGAGG
O598	mCherry-R(L_HA)	AGCGTAATCTGGTACGTCGTATGGGTACTTGTACAGCTCGTCCATGC
O599	HA-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCAAGCGTAATCTGGTACGTCGTATG
O600	mCherry(CE_mN1c)	TCACGAAAAAGAAGAAGGATGGTGAGCAAGGGCGAGG
O601	mN1c-R(M_S1)	TCGAGTTCTGCAGCTGCAGCGCCACCACTCGTGCCCGG
O602	mN1c-F(M_S1)	GCTGCAGCTGCAGAACTCGATCCTATGGACATACGGGGGTCC
O603	mN1c-R(M_S2)	GTTCGCTTTTGTGCACTTCGATTTTATACGGAATATTCAAGCTCCCAAGAGAGGCC
O604	mN1c-F(M_S2)	CGAAGTGCACAAAAGCGAACCTGTCGAACCGCC
O605	CD8aSP-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG
O606	PDGFRB-R	GCGAGGTTTCTTTTGCCAG
O607	mCherry-F(CE_PDGFRB)	TGGCAAAAGAAACCTCGCATGGTGAGCAAGGGCGAGG
O608	mCherry-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCACTTGTACAGCTCGTCCATGC
O609	tTAA-F(CE_PDGFRB)	TCTGGCAAAAGAAACCTCGCATGAGTCGATTGGATAAATCTAAGGTAATTAACAG
O610	minCMV-R(L1_ZF21-16)	cgcTACTAGTAtCACTTCTAAttCACTTCTAAtCTCTAgTAggcgatctgacggttcac
O611	minCMV-R(L2_ZF21-16)	CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGTAtCACTTCTAAttCAC
O612	minCMV-R(L1_ZF43-8)	cgcTACTAGTAgTCCTCACTCtgTCCTCACTCtCTCTAgTAGGCGATCTGACGGTTCAC
O613	minCMV-R(L2_ZF43-8)	CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGTAgTCCTCACTCtg
O614	tTAA-R-N(S)	CCCCTCGATACCTACTTCGTTC
O615	UAS-R(CE_minCMV）	CTCTAGTActagagtctccgctcggaggacaga
O616	minCMV-F(CE_UAS)	cggagactctagTACTAGAGGGTAGGCGTGTACGGTGG
O617	pSV40-R(L_ZF43-8)	cgcTACTAGTAgTCCTCACTCtgTCCTCACTCtCTCTAgTAtttgcaaaagcctaggcc
O618	mNc F (OE-test)	ATTCTCGACTACAGTTTCACAGGAG
O619	mNc R (OE-test)	CCTTCTTCTTTTTCGTGACAGCAAAA
O620	LNRA-R	GCAGTCACCCCCGTCC
O621	LNRB-F	TGCACCCAGTCCCTCCAAT
O622	LNRA R (OE_AB lnkr-WT10)	GTTCTTCCACGGATCATTGAAGTTAAG
O623	LNRB F (OE_AB lnkr-WT10)	TCACTTAACTTCAATGATCCGTGGAAG
O624	LNR A R (OE_ABlnkr-WT 8)	CTTCCACGGATCATTGAAGTTAAGTG
O625	LNR B F (OE_AB lnkr-WT8)	CTTAACTTCAATGATCCGTGGAAGAAC
O626	LNR A R (OE_AB lnkr-WT6)	CCACGGATCATTGAAGTTAAGTGAG
O627	LNR B F (OE_AB lnkr-WT6)	AACTTCAATGATCCGTGGAAGAAC
O628	LNR A R (OE_AB lnkr-WT4)	CGGATCATTGAAGTTAAGTGAGCAG
O629	LNR B F (OE_AB lnkr-WT4)	TTCAATGATCCGTGGAAGAACTGC
O630	LNR A R (OE_AB lnkr-WT2)	ATCATTGAAGTTAAGTGAGCAGTCACC
O631	LNR B F (OE_AB lnkr-WT2)	AATGATCCGTGGAAGAACTGCAC
O632	LNR A-lnkr R(OE mut L-I )	AGTTCTTCCACGGATCATTGAAGTTGATTGAGCAGTCACCCCCGT
O633	LNR B-lnkr F(OE mut L-I )	ACGGGGGTGACTGCTCAATCAACTTCAATGATCCGTGGAAGAACT
O634	LNR A-lnkr R(OE mut L-V)	AGTTCTTCCACGGATCATTGAAGTTCACTGAGCAGTCACCCCCGT
O635	LNR B-lnkr F(OE mut L-V )	ACGGGGGTGACTGCTCAGTGAACTTCAATGATCCGTGGAAGAACT
O636	LNR A-lnkr R(OE mut L-A)	AGTTCTTCCACGGATCATTGAAGTTGGCTGAGCAGTCACCCCCGT
O637	LNR B-lnkr F(OE mut L-A )	ACGGGGGTGACTGCTCAGCCAACTTCAATGATCCGTGGAAGAACT
O638	LNR A-lnkr R(OE mut L-G)	AGTTCTTCCACGGATCATTGAAGTTGCCTGAGCAGTCACCCCCGT
O639	LNR B-lnkr F(OE mut L-G )	ACGGGGGTGACTGCTCAGGCAACTTCAATGATCCGTGGAAGAACT
O640	LNR A-lnkr R(OE mut S-G)	GTTCTTCCACGGATCATTGAAGTTAAGGCCGCAGTCACCCCCGTCC
O641	LNR B-lnkr F(OE mut S-G )	GGACGGGGGTGACTGCGGCCTTAACTTCAATGATCCGTGGAAGAAC
O642	LNR A-lnkr R(OE mut N-Q)	GCAGTTCTTCCACGGATCATTGAATTGAAGTGAGCAGTCACCCCC
O643	LNR B-lnkr F(OE mut N-Q )	GGGGGTGACTGCTCACTTCAATTCAATGATCCGTGGAAGAACTGC
O644	LNR A-lnkr R(OE mut N-V)	GCAGTTCTTCCACGGATCATTGAAAACAAGTGAGCAGTCACCCCC
O645	LNR B-lnkr F(OE mut N-V )	GGGGGTGACTGCTCACTTGTTTTCAATGATCCGTGGAAGAACTGC
O646	LNR A-lnkr R(OE mut S-A)	GTTCTTCCACGGATCATTGAAGTTAAGGGCGCAGTCACCCCCGTCC
O647	LNR B-lnkr F(OE mut S-A )	GGACGGGGGTGACTGCGCCCTTAACTTCAATGATCCGTGGAAGAAC
O648	IL10-F(L1)	TGCTTACATGCGGAGATGTGGAGGAGAATCCGGGACCAATGCATTCTAGCGCTCTTTTG
O649	IL10-F(L2)	GAAGGTAGGGGGTCATTGCTTACATGCGGAGATGTG
O650	TetOn3G-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGAGCCGACTGGACAAG
O651	mNc F(CE_LNR-A[-]hlx3+)√	ATTCTCGACTACAGTTTCACAAATCACGCCTGCGGTT
O652	LaG17 R(CE_G6-LNR-A[-]hlx3+)	ACCGCCTCCACCGCCTCCGGAAACGGTCACCTGTGTACC
O653	mNc F(CE_G6-LNR-A[-]hlx3+)	GGAGGCGGTGGAGGCGGTAATCACGCCTGCGGTTG
O654	mNc F(CE_G6-LNR-A[-])	GGAGGCGGTGGAGGCGGTTCACTTAACTTCAATGATCCGTGGAAG
O655	LNR A-lnkr R(OE mut L-I )	TTGAAGTTAAGTGAGCAGTCACCCCCGTCCCAAC
O656	LNR B-lnkr F(OE mut L-I )	GACTGCTCACTTAACTTCAATGATCCGTGGAAGAACTGCACCC
O657	LNR A-lnkr R(OE mut L-V)	TTGAAGTTCACTGAGCAGTCACCCCCGTCCCAAC
O658	LNR B-lnkr F(OE mut L-V )	GACTGCTCAGTGAACTTCAATGATCCGTGGAAGAACTGCACCC
O659	LNR A-lnkr R(OE mut L-A)	TTGAAGTTGGCTGAGCAGTCACCCCCGTCCCAAC
O660	LNR B-lnkr F(OE mut L-A )	GACTGCTCAGCCAACTTCAATGATCCGTGGAAGAACTGCACCC
O661	LNR A-lnkr R(OE mut L-G)	TTGAAGTTGCCTGAGCAGTCACCCCCGTCCCAAC
O662	LNR B-lnkr F(OE mut L-G )	GACTGCTCAGGCAACTTCAATGATCCGTGGAAGAACTGCACCC
O663	LNR A-lnkr R(OE mut S-G)	TTGAAGTTAAGGCCGCAGTCACCCCCGTCCCAACC
O664	LNR B-lnkr F(OE mut S-G )	GACTGCGGCCTTAACTTCAATGATCCGTGGAAGAACTGCACCC
O665	LNR A-lnkr R(OE mut N-Q)	TTGAACTGAAGTGAGCAGTCACCCCCGTCC
O666	LNR B-lnkr F(OE mut N-Q )	GACTGCTCACTTCAGTTCAATGATCCGTGGAAGAACTGCACCCAG
O667	LNR A-lnkr R(OE mut N-V)	TTGAACACAAGTGAGCAGTCACCCCCGTCC
O668	LNR B-lnkr F(OE mut N-V )	GACTGCTCACTTGTGTTCAATGATCCGTGGAAGAACTGCACCCAG
O669	LNR A-lnkr R(OE mut S-A)	TTGAAGTTAAGGGCGCAGTCACCCCCGTCCCAACC
O670	LNR B-lnkr F(OE mut S-A )	GACTGCGCCCTTAACTTCAATGATCCGTGGAAGAACTGCACCC
O671	LNR A-lnkr R(OE mut N-L)	TTGAACAGAAGTGAGCAGTCACCCCCGTCC
O672	LNR B-lnkr F(OE mut N-L )	GACTGCTCACTTCTGTTCAATGATCCGTGGAAGAACTGCACCCAG
O673	LNR A-lnkr R(OE mut N-G)	TTGAAGCCAAGTGAGCAGTCACCCCCGTCC
O674	LNR B-lnkr F(OE mut N-G )	GACTGCTCACTTGGCTTCAATGATCCGTGGAAGAACTGCACCCAG
O675	tTAA-F(CE_p2A)	GAAGAAAACCCGGGACCTATGAGTCGATTGGATAAATCTAAGGTAATTAACAG
O676	rtTAA-F(CE_p2A)	GAAGAAAACCCGGGACCTatgtctagactggacaagagcaaag
O677	CD19ECD-F(3-BamHI)	CTGGGATCCGAAGAGCCTCTCGTCGTGAAAG
O678	CD19ECD-R(TAA-EcoRI-3)	CGGGAATTCTTACTTCCATCCCCCCGTGC
O679	Gal4-R(CE_VP64)	agaAcctccActTccAccagcagctgcCACTGTCAGCTGCCGCTGgc
O680	VP64-F(CE_Gal4)	ggTggAagTggaggTtctggCgggAGcgatgctttagacgattttgacttagatatgc
O681	VP64-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCAtgatccGagcatGtcCagGtcGaagtcatctaggg
O682	Gal4-F(CE_P2A)	GAAGAAAACCCGGGACCTATGAAGCTGCTGAGCAGCatc
O683	mCherry-F-C(S)	ACCTCCCACAACGAGGAC
O684	SynTF-F-N(CE_P2A)	GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTG
O685	mScarlet-F(3-SalI-Kozak-ATG-BamHI)	CTAGTCGACGCCACCATGGGATCCGTGAGCAAGGGCGAGG
O686	mScarlet-R(M)	CTTGCGGTCcACGTTGTAGGCGCCG
O687	mScarlet-F(M)	TACAACGTgGACCGCAAGTTGGACATCAC
O688	G4S-R(C)	ACATCGGCCATTGAACCACCCCCACCGGAACC
O689	LaG17-F(CE_G4S)	TGGTTCAATGGCCGATGTGCAGC
O690	mN1c-F-N(C)	ATTCTCGACTACAGTTTCACAGGAGGAGCG
O691	HA-R(C)	CGCGTAATCTGGTACATCATAGGGGTAGGG
O692	LaG17-F(CE_HA)	GATGTACCAGATTACGCGATGGCCGATGTGCAGC
O693	LaG17-R	GGAAACGGTCACCTGTGTACC
O694	G4S-F(CE_LaG17)	ACACAGGTGACCGTTTCCGGGGGGGGTGGCAGTGG
O695	SynZF-F(CE_p2Y-Kozak)	ATCACGTTAGGGCTAGTCGACGCCACCATGGCAAGTCCTAGGGTG
O696	TRE3GV-F(1-BP_M_p2Y)	CGAATTCcggccgcttcTAGAGTCCCTATCagtgatagagaacgtatgaag
O697	4xUAS-F(1-BP_M_p2Y)	tGAATTCcggccgcttcTAGAGcgccaagctacggg
O698	4xUAS-R(1-BS_M_p2Y)	cACCGGTCTGCAGacggccgcTACTAGTAcgctcggaggacaga
O699	CMV-R(L1_21-43)	TCTAAtCTAGTAgTCCTCACTCttCACTTCTAAtCTAGTAagctctgcttatatagacc
O724	pol-F-C	GCCCTCAAAGTAGACGGC
O725	ori-F-3	CGCCTGGTATCTTTATAGTCCTG
O726	tTAA-R(C)	TCCTGGGAGCATATCCAGGTCAAGATCGTC
O727	CD8ASP-F(L1_P2A)	CTCCCTGCTGAAGCAAGCAGGAGACGTGGAAGAAAACCCGGGACCTATGGCGCTGCCGG
O728	CD8ASP-F(L2_P2A	CE_tTAA)	CTGGATATGCTCCCAGGAggctccggcGCCACCAACTTCTCCCTGCTGAAGCAAGC
O729	LaG2-F-N(S)	GTGCTGCCTCCGGTC
O730	tTAA-F-C(S)	GCTTAAGTGCGAGAGTGGC
O731	ACD8b-F-N(S)	TATCCGGGGTCTCCCTCC
O732	Gal4DBD-R-N(S)	ttggcgcacttgggc
O733	TetOn3G-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGGAGGTgaattcGCCACC
O734	TetOn3G-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTACCTCCggatccTTAGCC
O735	T2A-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACggAtcAggTGAGGGTCGG
O736	2.3D11scFv-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCACTTTATTTCGACTTTCGTGCCTCC
O737	CAR3G-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACCCAGCCAAACCAACAACTACAC
O747	CD8A-F(CE_P2A)	GAAGAAAACCCGGGACCTATGGCGCTGCCGG
O748	SynPro_space_5-F(CE_p2Y)	accggaacccttaatGAATTCCGGGTTTCGTAACAATCGCATGAGGATTCGCAACGC
O749	SynPro_space_7/7-F(CE_p2Y)	accggaacccttaatGAATTCGCCTAGATCATCGTTGGC
O750	SynPro_space_6/7-F(CE_p2Y)	accggaacccttaatGAATTCCGCACGTATCACTTGATC
O751	SynPro_space_5/7-F(CE_p2Y)	accggaacccttaatGAATTCCTTTCCACGATCATGTGC
O752	SynPro_space_4/4-F(CE_p2Y)	accggaacccttaatGAATTCGACTGTTGCGAACGATTC
O753	SynPro_space_3/4-F(CE_p2Y)	accggaacccttaatGAATTCTCCCGTCTCAGTAAAGG
O754	SynPro_space_2/4-F(CE_p2Y)	accggaacccttaatGAATTCAATCGGACTGCCTTCG
O755	SynPro_space_1/4-F(CE_p2Y)	accggaacccttaatGAATTCGTATCAGTCGCCTCGG
O756	minCMV2-F(L_space3)	CGTAAGAGGCTCACTCTCCCTTACACGGAGTGGATAGGTAGGCGTGTACGG
O757	minCMV2-R(CE_p2Y)	CAGTACAaTAAGACCACCGGTAAGTATAAGACAAAAGTGTTGTGG
O758	ZF21.16_8/8-F(L)	GAGGATTCGCAACGCaTTAGAAGTGaGCCTAGATCATCGTTGGC
O759	ZF21.16_7/8-F(L)	GCCTAGATCATCGTTGGCaTTAGAAGTGaCGCACGTATCACTTGATC
O760	ZF21.16_6/8-F(L)	CGCACGTATCACTTGATCaTTAGAAGTGaCTTTCCACGATCATGTGC
O761	ZF21.16_5/8-F(L)	CTTTCCACGATCATGTGCaTTAGAAGTGaGACTGTTGCGAACGATTC
O762	ZF21.16_4/8-F(L)	GACTGTTGCGAACGATTCaTTAGAAGTGaTCCCGTCTCAGTAAAGG
O763	ZF21.16_3/8-F(L)	TCCCGTCTCAGTAAAGGTaTTAGAAGTGaAATCGGACTGCCTTCG
O764	ZF21.16_2/8-F(L)	AATCGGACTGCCTTCGTAaTTAGAAGTGaGTATCAGTCGCCTCGG
O765	ZF21.16_1/8-F(L)	GTATCAGTCGCCTCGGAAaTTAGAAGTGaCGTAAGAGGCTCACTCTC
O766	TetO_6/6-F(L)	CGCACGTATCACTTGATCTCCCTATCagtgatagagaCTTTCCACGATCATGTGC
O767	TetO_5/6-F(L)	CTTTCCACGATCATGTGCTCCCTATCagtgatagagaGACTGTTGCGAACGATTC
O768	TetO_4/6-F(L)	GACTGTTGCGAACGATTCTCCCTATCagtgatagagaTCCCGTCTCAGTAAAGG
O769	TetO_3/6-F(L)	TCCCGTCTCAGTAAAGGTTCCCTATCagtgatagagaAATCGGACTGCCTTCG
O770	TetO_2/6-F(L)	AATCGGACTGCCTTCGTATCCCTATCagtgatagagaGTATCAGTCGCCTCGG
O771	TetO_1/6-F(L)	GTATCAGTCGCCTCGGAATCCCTATCagtgatagagaCGTAAGAGGCTCACTCTC
O772	minCMV2-F(CE_p2Y)	accggaacccttaatGAATTCGGTAGGCGTGTACGGTGG
O773	SV40NLS-KRAB-F(CE_P2A)	GAAGAAAACCCGGGACCTATGGGCCCAAAAAAGAAGAGAAAGGTAatggacg
O774	KRAB-G4S-R	ACTACCTCCTCCACCTACAAGCCACGGTTCCTCGCccttttccagcctaag
O775	TetR-F(CE_KRAB-G4S)	TTGTAGGTGGAGGAGGTAGTATGAGTCGATTGGATAAATCTAAGG
O776	TetR-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCAAGAGCCACTCTCGCACTTAAG
O777	Gal4-F(CE_KRAB-G4S)	TTGTAGGTGGAGGAGGTAGTATGAAGCTGCTGAGCAGCatc
O778	Gal4-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCACACTGTCAGCTGCCGC
O779	SynZFN-F(CE_KRAB-G4S)	TTGTAGGTGGAGGAGGTAGTATGGCAAGTCCTAGGGTG
O780	ZF21.16-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTGTGTGTTTTCAGATGc
O781	ZF42.10-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTGTGGGTTTTCAGATGc
O782	ZF43.8-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTATGTGTTTTCAGATGtc
O783	7xTetO-R(CE_CMVe)	accgtaagttatgtaacgGCCTACCTCGACATACG
O784	4xUAS-F(CE_p2Y)	accggaacccttaatGAATTCcgccaagctacggg
O785	4xUAS-R(CE_CMVe)	accgtaagttatgtaacgGTActagagtctccgc
O786	8xZFO-R(CE_CMVe)	accgtaagttatgtaacgCACGCCTACCCTCTAG
O787	VP64-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGgatgctttagacgattttgacttag
O788	VP64-G4S-R	ACTACCTCCTCCACCAagcatGtcCagGtcGaagtcatc
O789	SynZF-F(CE_VP64_G4S)	tgctTGGTGGAGGAGGTAGTatgGCAAGTCCTAGGGTG
O790	CfaN-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCATGGCAACCCATCGACCTG
O791	KRAB-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGgacgccaagagcctg
O792	SynZF-F(CE_KRAB_G4S)	TTGTAGGTGGAGGAGGTAGTatgGCAAGTCCTAGGGTG
O793	CfaC-F(CE_p2Y)	ATCACGTTAGGGCTAGTCGACGCCACCATGGTTAAGATCATATCACGG
O794	SynZF-R(SV40NLS-CE_p2Y)	TACTTGAATGAGGGAAGCTTTCATACCTTTCTCTTCTTTTTTGGGGAGCCCTTCTCACC
O795	KRAB-R(CE_NES)	GTCAATCTTTCCAGGGGCGGCAGTACAAGCCACGGTTCCTCGCccttttccagcctaag
O796	SynZF-F(L1_dNLS)	GATCAGAGAACTTGTATTTTCAAAGTGGCAAGAAGAAGAAAATGGCAAGTCCTAGGGTG
O797	SynZF-F(L2_dNLS)	GCCCCTGGAAAGATTGACGCTGAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC
O798	KRAB-R	TACAAGCCACGGTTCCTCGCccttttccagcctaag
O799	dNLS-F(CE_KRAB)	GAGGAACCGTGGCTTGTAAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC
O800	KRAB-R(CE_TCS)	CAAGTTCTCTGATCCACCTACAAGCCACGGTTCCTCGCccttttccagcctaag
O801	SynZF-F(CE_TCS)	GGTGGATCAGAGAACTTGTATTTTCAAAGTGGCGGAATGGCAAGTCCTAGGGTG
O802	TEVp-F(L1_NLS)	GGTGCCAAAAAAGAAGAGAAAGGTAGGGGAGAGCCTGTTCAAAG
O803	TEVp-F(L2_NLS)	ATCACGTTAGGGCTAGTCGACGCCACCATGGTGCCAAAAAAGAAGAGAAAGG
O804	TEVpN-R(CE_CfaN)	CGTGTCGTATGAGAGGCATGTCTGAAAATTAGTGGTTACCAAAC
O805	CfaN-F	TGCCTCTCATACGACACGGAAATTTTGACCGTC
O806	CfaN-R(CE_p2Y)	GTACTTGAATGAGGGAAGCTTTCATGGCAACCCATCGACC
O807	CfaC-F	ATCACGTTAGGGCTAGTCGACGCCACCATGGTTAAGATCATATCACGGAAGAGC
O808	CfaC-R	GTTTGAAGCGACGAGACCATTTTTAAGGAGG
O809	TEVpC-F(CE_CfaC)	GGTCTCGTCGCTTCAAACTGCGCCGAAAAGAGCATGTCCTCCATGG
O810	BS-R(CE_minCMV2)	ACGCCTACCTCGACATACGTCACGCCTACCCTCTAG
O811	minCMV2-F	ACGTATGTCGAGGTAGGCGT
O812	VP64-R	CagcatGtcCagGtcGaagtcatc
O813	dNLS-F(CE_VP64)	ttCgaCctGgaCatgctGAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC
O814	SynZFC-R(CE_T2AN)	CCTACCTTCAccTgaTccGGAGCCCTTCTCACCTGT
O815	SV40NLS-F(L1_T2A)	GCTTACATGCGGAGATGTGGAGGAGAATCCTGGACCAGTGCCAAAAAAGAAGAGAAAGG
O816	T2A-F	ggAtcAggTGAAGGTAGGGGGTCATTGCTTACATGCGGAGATGTGG
O817	TEVpC-R(NLS-CE_T2A)	CCTACCTTCAccTgaTccTACCTTTCTCTTCTTTTTTGGCTGGCTATAGACCAATTCG
O818	VP64-F(L_T2A)	TACATGCGGAGATGTGGAGGAGAATCCTGGACCAgatgctttagacgattttgacttag
O819	KRAB-F(L_T2A)	CTTACATGCGGAGATGTGGAGGAGAATCCTGGACCAATGgacgccaagagcc
O824	CE_p2Y-SalI(CE_pSB1C3-XbaI)	gaattcgcggccgcttctagagATCACGTTAGGGCTAGTCGAC
O825	CE_p2Y-HindIII(CE_pSB1C3-SpeI)	actgcagcggccgctactagtaGTACTTGAATGAGGGAAGCTT
O826	CE_p2Y-EcoRI(CE_pSB1C3-XbaI)	gaattcgcggccgcttctagagaccggaacccttaatGAATTC
O827	CE_p2Y-AgeI(CE_pSB1C3-SpeI)	actgcagcggccgctactagtaCAGTACAaTAAGACCACCGGT
O828	mN1c-F(CE_BP)	gaattcgcggccgcttctagagATTCTCGACTACAGTTTC
O829	mN1c-R(CE_BS)	actgcagcggccgctactagtaCCTTCTTCTTTTTCGTGA
O830	mN1ce-F(CE_BP)	gaattcgcggccgcttctagagCCTTGTGTTGGTAGTAATCC
O831	LaG2-F(CE_BP)	gaattcgcggccgcttctagagATGGCTCAGGTCCAGCTTG
O832	LaG2-R(CE_BS)	actgcagcggccgctactagtaAACAGTGACTTGCGTTCCC
O833	LaG16(CE_BP)	gaattcgcggccgcttctagagATGGCACAGGTCCAACTCG
O834	LaG16-R(CE_BS)	actgcagcggccgctactagtaGCTTACCGTTACTTGTGTGCC
O835	LaG17-F(CE_BP)	gaattcgcggccgcttctagagATGGCCGATGTGCAGC
O836	LaG17-R(CE_BS)	actgcagcggccgctactagtaGGAAACGGTCACCTGTGTACC
O837	ACD19-F(CE_BP)	gaattcgcggccgcttctagagGATATACAAATGACCCAGACTACGTCC
O838	ACD19-R(CE_BS)	actgcagcggccgctactagtaTGATGAAACGGTTACGGATGTCC
O839	CfaN-F(CE_BP)	gaattcgcggccgcttctagagTGCCTCTCATATGACACGG
O840	CfaN-R(CE_BS)	actgcagcggccgctactagtaTGGCAACCCATCGACCTG
O841	CfaC-F(CE_BP)	gaattcgcggccgcttctagagATGGTTAAGATCATATCACGGAAGAGC
O842	CfaC-R(CE_BS)	actgcagcggccgctactagtaGTTTGAAGCTACGAGACCATTTTTAAGG
O843	TEVN(CE_BP)	gaattcgcggccgcttctagagGGGGAGAGCCTGTTCAAAGG
O844	TEVpN(CE_BS)	actgcagcggccgctactagtaTGTCTGAAAATTAGTGGTTACCAAACAAATG
O845	TEVpC-F(CE_BP)	gaattcgcggccgcttctagagTGCGCCGAAAAGAGCATGTCCTCCATGGTTAG
O846	TEVpC-R(CE_BS)	actgcagcggccgctactagtaCTGGCTATAGACCAATTCGTTCATC
O847	SynZFN-F(CE_BP)	gaattcgcggccgcttctagagatgGCAAGTCCTAGGGTG
O848	ZF21.16N-R(CE_BS)	actgcagcggccgctactagtaCTGGAAGGGCTTTTCTCCG
O849	ZF21.16C-F(CE_BP)	gaattcgcggccgcttctagagTGTAGGATATGCATGCGAAACTTCTC
O850	SynZFC-R(CE_BS)	actgcagcggccgctactagtaGGAGCCCTTCTCACCTGT

gBlocks

> alphaCD19(CO)
tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG
CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCTACCCCTATGATGTACCAGAT
TACGCGGATATACAAATGACCCAGACTACGTCCAGCCTTTCCGCCTCCCTGGGTGATCGC
GTGACAATCAGTTGTAGGGCTAGTCAGGACATAAGTAAATACCTCAACTGGTATCAACAG
AAGCCTGACGGAACAGTTAAACTTCTTATCTATCATACAAGTAGGCTTCATTCAGGCGTT
CCATCCAGATTTAGTGGATCTGGGAGCGGCACCGACTACTCACTCACGATTAGTAATCTG
GAACAAGAGGACATCGCCACTTATTTTTGCCAGCAGGGTAACACTCTTCCCTATACATTC
GGCGGCGGTACGAAACTCGAAATTACAGGGGGGGGTGGCAGTGGTGGCGGAGGTTCCGGT
GGGGGTGGTTCAGAAGTAAAACTTCAGGAATCTGGTCCAGGGCTGGTTGCACCCAGCCAA
TCCCTTTCCGTCACGTGCACCGTATCCGGGGTCTCCCTCCCAGACTACGGCGTGAGTTGG
ATACGACAGCCGCCGCGCAAGGGGCTTGAGTGGCTGGGTGTCATTTGGGGATCTGAAACA
ACGTATTATAATTCCGCTTTGAAATCCCGCCTCACTATTATCAAGGACAATTCTAAGAGC
CAGGTCTTTCTGAAGATGAACAGCCTGCAAACGGATGACACAGCCATCTACTATTGTGCA
AAGCACTATTACTATGGTGGTTCATACGCTATGGATTACTGGGGACAAGGGACATCCGTA
ACCGTTTCATCACCCTGCGTCGGTAGCAACCC

> tTAA(CO)
TGTCACGAAAGCGCCGACGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAGCGCAC
TGGAGCTTCTGAACGAAGTAGGTATCGAGGGGCTCACCACACGAAAATTGGCACAGAAGC
TCGGAGTGGAGCAGCCGACTTTGTACTGGCACGTTAAGAATAAGAGGGCTTTGCTTGACG
CGCTGGCCATTGAGATGCTCGACCGGCATCACACTCACTTCTGCCCGCTTGAAGGCGAGA
GTTGGCAAGATTTCCTTAGGAACAATGCAAAGAGCTTTCGATGCGCCCTGCTTAGCCATC
GGGACGGCGCAAAGGTTCATCTTGGGACTCGGCCTACTGAGAAGCAATATGAAACCCTGG
AAAACCAGCTTGCTTTCCTTTGCCAACAGGGGTTCTCACTGGAGAATGCCCTGTACGCAC
TCTCCGCGGTCGGCCATTTTACTCTCGGGTGTGTTTTGGAAGATCAGGAACACCAGGTAG
CGAAAGAGGAGCGAGAAACGCCTACAACTGATTCCATGCCACCGTTGCTGAGACAAGCTA
TAGAGTTGTTCGACCACCAGGGTGCTGAACCGGCGTTTCTCTTTGGTCTGGAACTCATTA
TCTGCGGCCTTGAAAAGCAGCTTAAGTGCGAGAGTGGCGGTCCCGCAGATGCACTTGATG
ACTTTGATTTGGATATGTTGCCCGCGGACGCTCTTGATGACTTCGATCTGGATATGCTGC
CGGCTGATGCCCTCGACGATCTTGACCTGGATATGCTCCCAGGATGAaagcttggaaccc
ttaatataacttcgtat

> TetOn3G(CO)
GGAGGTgaattcGCCACCATGAGCCGACTGGACAAGAGCAAGGTAATCAATTCAGCTTTG
GAGCTTCTGAACGGCGTGGGAATCGAGGGTCTCACCACCAGAAAGCTCGCGCAAAAATTG
GGGGTGGAACAACCGACTCTCTATTGGCATGTTAAGAACAAGAGGGCCCTCCTTGACGCA
CTCCCCATCGAAATGCTGGATCGACATCACACACATAGTTGTCCGCTGGAGGGGGAGTCT
TGGCAAGATTTCCTCAGGAATAACGCGAAGAGTTATCGGTGCGCTCTCCTTAGTCATCGC
GATGGAGCGAAAGTGCATCTGGGGACAAGACCGACGGAAAAACAATATGAGACGTTGGAG
AACCAACTGGCATTCTTGTGCCAACAGGGGTTCAGCCTTGAAAATGCTCTTTACGCCTTG
AGCGCTGTTGGGCACTTCACCTTGGGCTGTGTACTGGAGGAGCAGGAGCACCAGGTCGCA
AAGGAGGAGCGCGAAACGCCCACCACAGATAGTATGCCACCCCTGTTGAAGCAGGCTATC
GAGCTTTTTGATCGGCAGGGTGCTGAGCCTGCCTTTTTGTTCGGCCTTGAGCTTATAATT
TGCGGCCTGGAAAAACAACTCAAGTGCGAAAGCGGTGGTCCCACAGACGCATTGGACGAT
TTTGATCTTGACATGCTGCCAGCCGATGCTTTGGACGATTTTGACCTGGATATGTTGCCG
GCAGATGCCCTCGACGACTTTGATCTTGATATGTTGCCGGGCTAAggatccGGAGGT

> mN1ce(CO)
CCCTGCGTCGGTAGCAACCCCTGCTACAATCAAGGGACCTGCGAGCCAACGTCAGAAAAT
CCCTTTTATAGATGCCTGTGCCCGGCAAAGTTCAACGGACTGCTGTGCCACATACTCGAC
TACAGTTTTACTGGGGGGGCGGGCCGGGATATACCCCCGCCTCAGATAGAAGAGGCATGC
GAACTGCCGGAATGCCAGGTCGATGCGGGCAATAAAGTCTGCAACCTCCAATGCAATAAT
CACGCCTGCGGTTGGGACGGGGGTGACTGCTCACTTAACTTCAATGATCCGTGGAAGAAC
TGCACCCAGTCCCTCCAATGTTGGAAATATTTTTCCGATGGTCATTGTGATAGCCAGTGC
AATTCCGCAGGATGTTTGTTCGATGGGTTTGACTGCCAACTGACAGAGGGTCAGTGTAAT
CCTCTTTACGACCAATACTGCAAGGATCATTTCAGTGATGGACATTGTGACCAAGGTTGT
AACAGTGCGGAGTGCGAATGGGATGGGTTGGATTGTGCTGAGCATGTTCCTGAGCGCCTC
GCAGCGGGAACTTTGGTCCTCGTTGTCCTCCTTCCGCCTGACCAGCTCAGAAATAATAGC
TTTCACTTTCTTAGAGAACTCTCTCATGTGTTGCATACCAACGTAGTCTTTAAGCGCGAC
GCTCAAGGACAGCAAATGATCTTCCCGTACTATGGGCACGAAGAAGAGCTTCGGAAACAC
CCGATAAAAAGGAGTACGGTTGGTTGGGCGACGAGTAGTCTTTTGCCGGGCACGAGTGGT
GGCAGACAACGGCGGGAACTCGATCCGATGGACATACGGGGGTCCATAGTGTATCTGGAG
ATTGACAACCGACAGTGTGTTCAAAGTTCAAGCCAGTGTTTTCAATCAGCCACTGATGTA
GCTGCGTTTCTCGGAGCGTTGGCCTCTCTTGGGAGCTTGAATATTCCGTATAAAATCGAA
GCTGTAAAAAGCGAACCTGTCGAACCGCCATTGCCAAGCCAGTTGCACCTCATGTACGTG
GCCGCTGCTGCGTTCGTACTCTTGTTCTTCGTAGGCTGCGGTGTTTTGCTGTCACGAAAG
CGCCGACGG

> hN1c(CO)
CCCTGTCTTGGAGGGAATCCGTGTTACAACCAAGGGACATGTGAACCCACGAGCGAGTCT
CCTTTTTACAGGTGTCTCTGTCCAGCAAAATTTAATGGATTGCTTTGCCACATTCTGGAT
TACTCATTTGGTGGGGGAGCGGGGCGGGACATACCGCCGCCCCTTATTGAAGAAGCATGC
GAACTGCCCGAGTGCCAGGAGGATGCAGGTAACAAGGTATGCTCTCTTCAGTGCAATAAC
CACGCTTGCGGGTGGGATGGTGGCGATTGCTCCTTGAATTTTAATGATCCTTGGAAAAAT
TGCACCCAATCACTTCAGTGTTGGAAATACTTCTCAGATGGCCATTGCGATTCTCAGTGT
AACAGCGCGGGATGCTTGTTTGACGGATTCGACTGTCAAAGGGCTGAGGGACAATGTAAT
CCGCTCTACGATCAGTATTGTAAAGACCATTTCAGCGACGGCCACTGTGACCAAGGCTGC
AATAGTGCCGAGTGTGAGTGGGATGGGTTGGATTGCGCGGAGCATGTTCCGGAGAGACTT
GCGGCAGGTACGCTCGTGGTTGTTGTACTCATGCCGCCTGAGCAACTGAGGAATAGTAGC
TTTCACTTCCTCCGGGAACTGAGCCGGGTTCTGCATACTAACGTTGTATTTAAGAGAGAT
GCACATGGTCAGCAAATGATATTTCCGTACTACGGTCGGGAAGAGGAATTGCGGAAGCAC
CCCATTAAGAGAGCTGCCGAGGGTTGGGCCGCTCCGGATGCCCTCCTTGGACAAGTAAAA
GCCTCCCTCCTTCCCGGCGGATCTGAGGGTGGACGGAGACGCCGGGAACTCGATCCAATG
GATGTAAGAGGGTCCATTGTGTACTTGGAGATTGATAATCGCCAGTGTGTGCAGGCGTCC
TCACAATGTTTTCAAAGTGCGACCGATGTCGCAGCATTCTTGGGTGCCCTGGCATCATTG
GGCAGCCTGAATATTCCATATAAGATCGAAGCGGTGCAATCCGAAACGGTGGAACCGCCT
CCACCGGCACAGCTTCATTTCATGTATGTCGCAGCAGCAGCTTTCGTTTTGCTTTTCTTC
GTAGGGTGTGGTGTTCTCCTTAGTCGAAAACGACGCCGG

> LaG17(CO)
tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG
CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG
GAGGACCTCATGGCCGATGTGCAGCTCGTCGAGTCAGGTGGGGGTCTCGTACAAGCTGGG
GGGTCACTCAGACTGTCTTGCGCCGCCAGTGGCAGAACCATCTCTATGGCAGCTATGTCC
TGGTTCAGACAGGCGCCAGGCAAGGAGAGGGAGTTTGTGGCGGGTATAAGTAGGTCCGCA
GGAAGCGCGGTCCACGCCGATTCAGTCAAAGGTCGGTTCACGATAAGCCGGGATAACACT
AAGAACACTCTTTATCTCCAGATGAACTCTCTGAAGGCCGAGGATACGGCTGTTTACTAT
TGCGCTGTACGGACATCTGGGTTCTTCGGGAGCATACCGCGCACCGGAACAGCGTTTGAT
TACTGGGGACAAGGTACACAGGTGACCGTTTCCCCCTGCGTCGGTAGCAACCC

> LaG16-2(CO)
tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG
CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG
GAGGACCTCATGGCACAGGTCCAACTCGTCGAATCCGGAGGTCGCCTCGTGCAAGCTGGC
GATTCACTTCGACTCTCTTGCGCGGCGTCAGGTCGGACCTTCAGCACGTCAGCTATGGCG
TGGTTCCGCCAGGCGCCTGGGAGAGAGAGAGAGTTTGTAGCAGCCATCACATGGACTGTT
GGAAATACTATATTGGGCGACTCCGTAAAAGGGCGCTTCACTATCTCACGAGATAGAGCG
AAAAACACTGTAGATCTGCAAATGGACAATTTGGAGCCGGAGGATACTGCTGTGTATTAT
TGCAGCGCTCGCTCTCGGGGGTACGTCCTCTCCGTACTCCGGAGCGTGGATTCATACGAC
TACTGGGGACAGGGCACACAAGTAACGGTAAGCGGTGGGGGAGGGTCTGGTGGTGGAGGC
TCAGGAGGCGGGGGATCTATGGCTCAGGTCCAGCTTGTAGAGAGCGGTGGTGGATTGGTG
CAAGCAGGAGGGAGCCTCAGGCTTAGTTGTGCTGCCTCCGGTCGAACCTTCTCAAATTAT
GCAATGGGTTGGTTTAGGCAAGCTCCTGGTAAAGAACGGGAGTTCGTGGCCGCAATTAGC
TGGACGGGAGTTAGCACATATTACGCTGACTCTGTGAAAGGTCGGTTCACGATAAGTCGA
GACAATGATAAGAATACGGTTTACGTCCAAATGAATAGCCTCATCCCGGAGGACACAGCT
ATATATTATTGCGCTGCTGTACGGGCAAGATCCTTCTCAGATACTTATAGCAGGGTTAAC
GAATACGATTATTGGGGGCAGGGAACGCAAGTCACTGTTCCCTGCGTCGGTAGCAACCC

> EGFPtm(CO)
GGAAGAAAATCCCGGTCCGATGGCCCTTCCTGTAACGGCGCTCCTTCTCCCTCTTGCCC
TCCTGCTCCATGCCGCACGACCAATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGG
TGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCG
AGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA
AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCA
GCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCT
ACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGG
TGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGG
AGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATA
TCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCG
AGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCC
CCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCA
ACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCG
GCATGGACGAGCTGTACAAGAATGCCGTTGGTCAAGACACGCAGGAAGTCATTGTTGTAC
CCCACTCCCTGCCATTTAAGGTGGTAGTAATTTCAGCCATCCTTGCTCTCGTTGTGTTGA
CAATTATTTCTCTGATAATTCTTATCATGCTCTGGCAAAAGAAACCTCGCTGAaagcttg
gaacccttaatataacttcgtat

> CD19ECD(CO)
TTGCTCCCACTGGCCTTGCTGCTTCATGCGGCACGGCCTTACCCCTATGATGTACCAGAT
TACGCGGAAGAGCCTCTCGTCGTGAAAGTTGAAGAAGGTGACAATGCAGTTTTGCAATGT
CTGAAAGGGACGTCTGACGGACCAACCCAACAGCTCACTTGGAGTCGCGAATCCCCGCTT
AAGCCGTTCCTGAAGTTGTCTCTGGGGTTGCCGGGTCTTGGTATACACATGCGCCCGCTT
GCGATATGGCTCTTCATCTTCAATGTCAGCCAACAGATGGGCGGGTTCTATCTCTGTCAA
CCGGGACCGCCATCAGAGAAGGCGTGGCAACCTGGTTGGACGGTAAACGTGGAAGGTAGC
GGGGAGTTGTTTCGCTGGAACGTTTCTGATCTCGGGGGCCTGGGTTGCGGTCTGAAAAAT
CGCTCTAGCGAAGGGCCGAGTTCACCTTCCGGCAAGCTCATGTCCCCGAAACTCTACGTG
TGGGCGAAGGATAGGCCAGAAATTTGGGAGGGAGAACCCCCCTGCCTGCCGCCCAGAGAT
TCACTCAACCAGAGTCTCTCCCAAGATCTGACTATGGCCCCAGGAAGTACCTTGTGGCTT
AGCTGCGGTGTACCGCCCGACTCCGTGAGCCGAGGGCCTCTCTCTTGGACACACGTTCAC
CCTAAGGGGCCGAAATCATTGTTGAGTTTGGAACTCAAAGACGATAGGCCAGCAAGGGAT
ATGTGGGTTATGGAGACCGGCCTTCTGCTCCCACGAGCGACCGCTCAGGATGCGGGCAAG
TATTATTGCCACAGAGGAAATCTGACTATGTCTTTTCATCTGGAAATTACTGCGCGGCCA
GTTTTGTGGCACTGGCTGTTGCGCACGGGGGGATGGAAGGAGCAGAAATTGATCTCTGA

> Gal4DBD(CO)
atgaagctgctgagcagcatcgagcaggcctgtgacatctgccggctgaagaaactgaag
tgcagcaaagaaaagcccaagtgcgccaagtgcctgaagaacaactgggagtgccggtac
agccccaagaccaagagaagccccctgaccagagcccacctgaccgaggtggaaagccgg
ctggaaagactggaacagctgtttctgctgatcttcccacgcgaggacctggacatgatc
ctgaagatggacagcctccaggacatcaaggccctgctgaccggcctgttcgtgcaggac
aacgtgaacaaggacgccgtgaccgacagactggccagcgtggaaaccgacatgcccctg
accctgcggcagcacagaatcagcgccaccagcagcagcgaggaaagcagcaacaagggc
cagcggcagctgacagtgtct

> ZF21.16(CO)
GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCTCCTCACGAGAGGCCTTTTCAATGT
AGAATTTGTATGCGTAATTTTTCTaggaacttcatcctccagagaCATACAAGGACTCAC
ACAGGTGAAAAGCCTTTTCAGTGCAGAATTTGTATGCGCAATTTTTCTcagggcggtaac
ctggtgagaCATCTGCGTACCCACACTGGTGAAAAGCCTTTCCAATGTAGAATTTGTATG
CGAAATTTTTCTcagcaaaccggcctgaacgtgCATCTGAAGACACACACAGGCGAGAAG
GGATCC

> ZF42.10(CO)
GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCACCACATGAAAGACCATTCCAATGT
AGAATTTGTATGAGGAATTTTTCTaccggccagatcctggacagaCATACAAGGACCCAC
ACCGGCGAAAAGCCATTCCAGTGTAGAATTTGTATGCGTAATTTTTCTgtggcccacagc
ctgaagagaCATCTGAGGACCCACACCGGAGAGAAGCCTTTTCAATGCAGAATTTGTATG
CGTAATTTTTCTgaccccagcaacctgagaaggCATCTGAAGACCCACACCGGAGAAAAG
GGATCC

> ZF43.8(CO)
GCTAGCCCCAGAGTGAGAACCGGTTCTAAGACACCTCCCCACGAGAGGCCTTTTCAGTGT
AGAATTTGTATGCGTAATTTTTCTaggcaggacagactggacaggCACACAAGGACTCAC
ACTGGTGAAAAGCCCTTCCAATGTAGAATTTGTATGCGCAATTTTTCTcagaaggagcac
ctggccggcCATCTGAGGACCCACACAGGCGAAAAGCCTTTCCAGTGCAGAATTTGTATG
AGAAATTTTTCTaggagagacaacctgaacagaCATCTGAAGACACATACAGGTGAAAAA
GGATCC

> TEVp(FL)(CO)
ATCACGTTAGGGCTAGTCGACGCCACCATGGGGGAGAGCCTGTTCAAAGGGCCCCGCGAT
TATAACCCTATAAGCAGTACAATATGTCATCTGACTAACGAATCAGACGGACATACGACC
TCCCTCTACGGGATAGGGTTCGGCCCCTTCATCATCACCAATAAGCACTTGTTTAGGCGA
AATAACGGCACGCTTCTGGTACAGTCCCTGCACGGGGTCTTTAAGGTCAAGAATACGACA
ACTCTCCAACAGCATCTTATTGATGGACGCGACATGATTATCATTAGAATGCCTAAAGAT
TTTCCGCCGTTCCCACAAAAATTGAAGTTTAGAGAACCGCAAAGGGAAGAGCGCATTTGT
TTGGTAACCACTAATTTTCAGACAAAGAGCATGTCCTCCATGGTTAGTGACACTTCATGC
ACGTTTCCTTCTTCTGATGGTATATTTTGGAAACATTGGATACAGACGAAAGACGGACAG
TGCGGGTCCCCTCTTGTCTCTACCAGGGACGGCTTTATCGTCGGCATACATTCAGCCAGT
AATTTCACAAACACAAATAATTATTTTACTAGCGTGCCCAAAAATTTCATGGAGTTGCTG
ACCAATCAAGAGGCACAGCAGTGGGTTTCTGGATGGAGACTCAACGCCGACAGCGTACTC
TGGGGTGGACATAAGGTGTTCATGgtgAAACCCGAGGAACCCTTTCAGCCGGTGAAAGAG
GCCACGCAACTGATGAACGAATTGGTCTATAGCCAGTGAAAGCTTCCCTCATTCAAGTAC

> ZF21_16N-CfaN-CfaC-ZF21_16C(CO)
ATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGCGGACCGGATCAAAG
ACGCCGCCTCACGAACGACCATTTCAATGCAGGATCTGCATGCGGAACTTCTCCCGGAAC
TTTATCCTCCAGCGGCATACACGGACCCATACCGGAGAAAAGCCCTTCCAGTGCCTCTCA
TATGACACGGAAATTTTGACCGTCGAATATGGCTTTCTTCCTATCGGGAAGATCGTTGAA
GAAAGAATTGAATGCACGGTCTATACCGTGGATAAGAATGGTTTTGTATACACTCAACCG
ATAGCGCAGTGGCATAACAGGGGGGAGCAAGAAGTCTTTGAATACTGTTTGGAGGATGGG
TCCATCATCAGGGCAACCAAGGACCATAAGTTCATGACCACTGATGGGCAGATGCTTCCG
ATTGATGAAATTTTCGAAAGGGGCCTTGACCTGAAGCAGGTCGATGGGTTGCCAATGGTT
AAGATCATATCACGGAAGAGCCTGGGGACACAGAATGTGTATGATATTGGAGTAGGCGAG
CCGCACAACTTCCTCCTTAAAAATGGTCTCGTAGCTTCAAACTGTAGGATATGCATGCGA
AACTTCTCCCAGGGGGGCAACTTGGTCAGGCACCTTCGGACGCATACCGGAGAGAAGCCA
TTTCAGTGCAGGATTTGCATGCGAAATTTTTCTCAGCAAACCGGGTTGAATGTACATCTG
AAAACGCATACAGGTGAGAAGGGCTCCTGAAAGCTTCCCTCATTCAAGTAC

Abstract

Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.

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 <tr><th>#</th><th>Oligo Name</th><th>DNA Sequence (5 to 3)</th></tr>

Difference between revisions of "Team:Fudan/Primers"

Revision as of 17:41, 17 October 2018

Primers used

Primers used

Primers

gBlocks

Abstract