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<h4>Growth of msbB and RecBCD-Nuklease deficient E.Coli mutant strains</h4>
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<h4>Growth of msbB and RecBCD-Nuklease Deficient E.Coli Mutant Strains</h4>
 
<em>2018/08/28</em>
 
<em>2018/08/28</em>
 
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<h4>preparing electro-competent cells of WT RecBCD und dRecB</h4>
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<h4>Preparing Electro-Competent Cells of WT RecBCD und dRecB</h4>
 
<em>2018/08/31</em>
 
<em>2018/08/31</em>
 
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<h4>DNA midi prep of RecBCD(WT) plasmid from Dh5a</h4>
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<h4>DNA Midi Prep of RecBCD(WT) Plasmid from Dh5a</h4>
 
<em>2018/09/03</em>
 
<em>2018/09/03</em>
 
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<h4>Growth of RecB, RecD, RecBCD, msb�-B deficient E.Coli strains</h4>
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<h4>Growth of RecB, RecD, RecBCD, msb�-B Deficient E.Coli Strains</h4>
 
<em>2018/09/14</em>
 
<em>2018/09/14</em>
 
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Latest revision as of 00:43, 18 October 2018

Growth of msbB and RecBCD-Nuklease Deficient E.Coli Mutant Strains

2018/08/28
Participants: Katja Neishsalo
Protocol: Measuring optical density
Notes: Strains tested: deltamsbB; DeltaRecB, DeltaRecD and WT (MG1655); inoculation of 100 ml LB medium with 1 ml preculture. Measure OD every 30 – 60 minutes.
Results:

Preparing Electro-Competent Cells of WT RecBCD und dRecB

2018/08/31
Participants: Katja Neishsalo
Protocol: Preparation electrocompetent cells
Results: PICcells are still viable after preparation, dRecB grows significantly slower than others

Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)

2018/09/03
Participants: Katja Neishsalo
Protocol: Electrocompetent transformation
Results: Colonies

DNA Midi Prep of RecBCD(WT) Plasmid from Dh5a

2018/09/03
Participants: Katja Neishsalo
Protocol: Midiprep

Growth of RecB, RecD, RecBCD, msb�-B Deficient E.Coli Strains

2018/09/14
Participants: Katja Neishsalo
Protocol: Measuring optical density
Notes:
Results:
The experiment shows no significant difference between growth curves of KO strains compared to those with RecBCDwt-Plasmid. The reference curves (strains transformed with mRFP) have the highest OD values. We suppose that mRFP falsified the OD values since it also emits fluorescence in the wavelength of the OD measurement.