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<p> Our project was made possible by help from a variety of different sources: </p> | <p> Our project was made possible by help from a variety of different sources: </p> | ||
<p> - Funding from geekStarter/Mindfuel, Knights of Columbus High River, Cargill High River, and Co-op High River. This allowed us to purchase our genetic circuit, purchase lab reagents and equipment as well as travel to places such as aGEM 2018, and by the end of October, iGEM 2018.</p> | <p> - Funding from geekStarter/Mindfuel, Knights of Columbus High River, Cargill High River, and Co-op High River. This allowed us to purchase our genetic circuit, purchase lab reagents and equipment as well as travel to places such as aGEM 2018, and by the end of October, iGEM 2018.</p> | ||
− | <p> - Throughout the process, our mentors, FREDsense’s Emily and Robert, were invaluable in helping us with the execution of different lab procedures as well as providing us with advice which helped moved our project forward. In addition to this, Robert taught us modelling and provided the idea of testing our bacteria using Nitrophenol | + | <p> - Throughout the process, our mentors, FREDsense’s Emily and Robert, were invaluable in helping us with the execution of different lab procedures as well as providing us with advice which helped moved our project forward. In addition to this, Robert taught us modelling and provided the idea of testing our bacteria using Nitrophenol so that we could use a spectrophotometer to quantitatively model the reaction and use those values for our model. Without Emily, we would not have successfully inserted our gene into the proper pSB1C3 backbone to submit our new part into the registry in time.</p> |
<p> - Cassie Sillner, Jacob Grainger, Christian Emond, and Lalit Bharadwaj from the University of Calgary iGEM team helped us conduct testing with their spectrophotometer. Rainer Lim helped us in designing a logo and other graphic design aspects of our project.</p> | <p> - Cassie Sillner, Jacob Grainger, Christian Emond, and Lalit Bharadwaj from the University of Calgary iGEM team helped us conduct testing with their spectrophotometer. Rainer Lim helped us in designing a logo and other graphic design aspects of our project.</p> | ||
<p> - During aGEM, we were able to gain valuable responses from the judges and other participants, which helped us prepare for iGEM. It was also during aGEM that we were able to contact Amino Labs and University of Calgary iGEM team who are helping us to improve our presentation.</p> | <p> - During aGEM, we were able to gain valuable responses from the judges and other participants, which helped us prepare for iGEM. It was also during aGEM that we were able to contact Amino Labs and University of Calgary iGEM team who are helping us to improve our presentation.</p> |
Revision as of 21:55, 17 October 2018
Attributions
The idea for our project, degrading fatbergs, was generated by one of our team members, Miguel, during one of our meetings. Our team then did research in the literature to determine what genes might be able to break down fats. Our mentors from FREDsense Technologies, Emily and Robert, helped us find relevant papers, identify our EstA gene and eventually design our genetic circuit, which we had synthesized by BioBasic.
Our project was made possible by help from a variety of different sources:
- Funding from geekStarter/Mindfuel, Knights of Columbus High River, Cargill High River, and Co-op High River. This allowed us to purchase our genetic circuit, purchase lab reagents and equipment as well as travel to places such as aGEM 2018, and by the end of October, iGEM 2018.
- Throughout the process, our mentors, FREDsense’s Emily and Robert, were invaluable in helping us with the execution of different lab procedures as well as providing us with advice which helped moved our project forward. In addition to this, Robert taught us modelling and provided the idea of testing our bacteria using Nitrophenol so that we could use a spectrophotometer to quantitatively model the reaction and use those values for our model. Without Emily, we would not have successfully inserted our gene into the proper pSB1C3 backbone to submit our new part into the registry in time.
- Cassie Sillner, Jacob Grainger, Christian Emond, and Lalit Bharadwaj from the University of Calgary iGEM team helped us conduct testing with their spectrophotometer. Rainer Lim helped us in designing a logo and other graphic design aspects of our project.
- During aGEM, we were able to gain valuable responses from the judges and other participants, which helped us prepare for iGEM. It was also during aGEM that we were able to contact Amino Labs and University of Calgary iGEM team who are helping us to improve our presentation.
- Patrick Wu gave us valuable feedback on our project, and helped us with different wiki problems we had.
- George Dearing helped us with notarizing travel documents for iGEM.
- Jayden Henderson took photographs of our team members for this wiki.
- Norm Durieux, a local retired electrical engineer helped us to create our own incubator using Christmas lights and a plastic container.