Difference between revisions of "Team:IIT Kanpur/Results"

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<h1 align="center">Results</h1>
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<h3>Cloning of our device for optimizing  extracellular expression using PelB/Ompt secretion tags under different Anderson promoters:</h3>
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We used  pSBIK3.m1 and pSB1C3 for cloning of our designed constructs. We synthesised  our DNA construct via IDT. We  added restriction enzyme site for Ecor1 and Pst1 in our synthesised double stranded DNA. We performed restriction enzyme  digestion for both our double stranded DNA and backbones with Ecor1 and Pst1. These were ligated together.
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These are the biobricks we submitted in iGEM registry.
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BBa_K2874000
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BBa_K2874001
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BBa_K2874002
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These are the sequences which we cloned but are not submitted with iGEM registry.
  
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pBS1K3.m1 ( JA23100->pelB-LINKER-sfGFP-HIS-STOP)
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pBS1K3.m1 ( JA23102->ompT-LINKER-sfGFP-HIS-STOP)
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Revision as of 23:34, 17 October 2018

Results

Cloning of our device for optimizing extracellular expression using PelB/Ompt secretion tags under different Anderson promoters:

We used pSBIK3.m1 and pSB1C3 for cloning of our designed constructs. We synthesised our DNA construct via IDT. We added restriction enzyme site for Ecor1 and Pst1 in our synthesised double stranded DNA. We performed restriction enzyme digestion for both our double stranded DNA and backbones with Ecor1 and Pst1. These were ligated together.

These are the biobricks we submitted in iGEM registry. BBa_K2874000 BBa_K2874001 BBa_K2874002

These are the sequences which we cloned but are not submitted with iGEM registry. pBS1K3.m1 ( JA23100->pelB-LINKER-sfGFP-HIS-STOP) pBS1K3.m1 ( JA23102->ompT-LINKER-sfGFP-HIS-STOP)