Difference between revisions of "Team:Vilnius-Lithuania/Basic"

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     <div class="modal-close"></div>
 
     <div class="modal-close"></div>
 
     <div class="modal-content">
 
     <div class="modal-content">
−
        <h1>Description</h1>
+
<h1>Basic Parts</h1>     
−
         <p></p>
+
<table class="c39">
−
        <p></p>
+
         <tbody>
−
        <h2>What is SynORI?</h2>
+
          <tr class="c9">
−
        <p>SynORI stands for synthetic origin of replication. It is a framework designed to make working with single
+
              <td class="c27" colspan="1" rowspan="1">
−
            and multi-plasmid systems precise, easy and on top of that - more functional.</p>
+
                <p class="c5"><span class="c12 c29">Part Number</span></p>
−
        <p>The SynORI framework enables scientists to build a multi-plasmid system in a standardized manner by:</p>
+
              </td>
−
        <ol>
+
              <td class="c25" colspan="1" rowspan="1">
−
            <li>Selecting the number of plasmid groups</li>
+
                <p class="c5"><span class="c12 c29">Type</span></p>
−
            <li>Choosing the copy number of each group</li>
+
              </td>
−
            <li>Picking the type of copy number control (specific to one group or regulating all of them at once).</li>
+
              <td class="c16" colspan="1" rowspan="1">
−
 
+
                <p class="c5"><span class="c12 c29">Name</span></p>
−
        </ol>
+
              </td>
−
        </p>
+
              <td class="c13" colspan="1" rowspan="1">
−
 
+
                <p class="c5"><span class="c12 c29">Brief description</span></p>
−
        <p></p>
+
              </td>
−
        <p>The framework also includes a possibility of adding a selection system that reduces the usage of antibiotics
+
              <td class="c32" colspan="1" rowspan="1">
−
            (only 1 antibiotic for up to 5 different plasmids!) and an active partitioning system to make sure that low
+
                <p class="c5"><span class="c12 c29">Length, nt</span></p>
−
            copy number plasmid groups are not lost during the division.
+
              </td>
−
        </p>
+
          </tr>
−
        <p></p>
+
          <tr class="c9">
−
        <div class="img-cont">
+
              <td class="c27" colspan="1" rowspan="1">
−
            <img src="https://static.igem.org/mediawiki/parts/8/84/Collect.png" alt="img">
+
                <p class="c5"><span class="c0">BBa_K2622001 </span></p>
−
            <div class="img-label">
+
              </td>
−
            </div>
+
              <td class="c25" colspan="1" rowspan="1">
−
        </div>
+
                <p class="c5"><span class="c0">Coding</span></p>
−
        <h2>Applications</h2>
+
              </td>
−
        <p>
+
              <td class="c16" colspan="1" rowspan="1">
−
            <h5>Everyday lab work</h5>
+
                <p class="c5"><span class="c43">BamB</span></p>
−
            <p>
+
              </td>
−
                A multi-plasmid system that is easy to assemble and control. With our framework the need to limit your
+
              <td class="c13" colspan="1" rowspan="1">
−
                research to a particular plasmid copy number just because there are not enough right replicons to
+
                <p class="c5"><span class="c0">Beta-barrel assembly protein B (BamB)</span></p>
−
                choose from, is eliminated. With SynORI you can easily create a vector with a desired copy number that
+
              </td>
−
                suits your needs.</li>
+
              <td class="c7" colspan="1" rowspan="1">
−
            </p>
+
                <p class="c24"><span class="c0">1261</span></p>
−
            <h5>Biological computing</h5>
+
              </td>
−
            <p>
+
          </tr>
−
                The ability to choose a wide range of copy number options and their control types will make the
+
          <tr class="c9">
−
                synthetic biology engineering much more flexible and predictable. Introduction of plasmid copy number
+
              <td class="c27" colspan="1" rowspan="1">
−
                regulation is equivalent to adding a global parameter to a computer system. It enables the coordination
+
                <p class="c5"><span class="c0">BBa_K2622002</span></p>
−
                of multiple gene group expression.
+
              </td>
−
            </p>
+
              <td class="c25" colspan="1" rowspan="1">
−
            <h5>Smart assembly of large protein complexes</h5>
+
                <p class="c5"><span class="c0">Coding</span></p>
−
            <p>
+
              </td>
−
                The co-expression of multi-subunit complexes using different replicons brings incoherency to an already
+
              <td class="c16" colspan="1" rowspan="1">
−
                chaotic cell system. This can be avoided by using SynORI, as in this framework every plasmid group uses
+
                <p class="c5 c37"><span class="c0">BamA</span></p>
−
                the same type of control, and in addition can act in a group-specific manner.</p>
+
              </td>
−
 
+
              <td class="c13" colspan="1" rowspan="1">
−
            <h5>Metabolic engineering</h5>
+
                <p class="c5"><span class="c0">Beta-barrel assembly protein A (BamA)</span></p>
−
            <p>
+
              </td>
−
                A big challenge for heterologous expression of multiple gene pathways is to accurately adjust the
+
              <td class="c7" colspan="1" rowspan="1">
−
                levels of each enzyme to achieve optimal production efficiency. Precise promoter tuning in
+
                <p class="c24"><span class="c0">2520</span></p>
−
                transcriptional control and synthetic ribosome binding sites in translational control are already
+
              </td>
−
                widely used to maintain expression levels. In addition to current approaches, our framework allows a
+
          </tr>
−
                simultaneous multiple gene control. Furthermore, an inducible regulation that we offer, can make the
+
          <tr class="c9">
−
                search for perfect conditions a lot easier.
+
              <td class="c27" colspan="1" rowspan="1">
−
 
+
                <p class="c5"><span class="c0">BBa_K2622003</span></p>
−
 
+
              </td>
−
 
+
              <td class="c25" colspan="1" rowspan="1">
−
            </p>
+
                <p class="c5"><span class="c0">Coding</span></p>
−
 
+
              </td>
−
 
+
              <td class="c16" colspan="1" rowspan="1">
−
        </p>
+
                <p class="c5"><span class="c0">IgA</span></p>
−
        <p>
+
              </td>
−
        </p>
+
              <td class="c13" colspan="1" rowspan="1">
−
        <table style="width:100%">
+
                <p class="c5"><span>Immunoglobulin protease A (IgA) beta-domain from </span><span class="c22">Neisseria gonorrhoeae</span></p>
−
<thead>
+
              </td>
−
<td align='center'>Species sign in ODE system</td>
+
              <td class="c7" colspan="1" rowspan="1">
−
<td align='center'>Species</td>
+
                <p class="c24"><span class="c0">1398</span></p>
−
<td align='center'>Initial concentration (M)</td>
+
              </td>
−
</thead>
+
          </tr>
−
<tbody>
+
          <tr class="c9">
−
<tr>
+
              <td class="c27" colspan="1" rowspan="1">
−
<td align='center'>A</td>
+
                <p class="c5"><span class="c0">BBa_K2622004</span></p>
−
<td align='center'>pDNA+RNA I+RNAII early</td>
+
              </td>
−
<td align='center'>0</td>
+
              <td class="c25" colspan="1" rowspan="1">
−
</tr>
+
                <p class="c5"><span class="c0">Coding</span></p>
−
<tr>
+
              </td>
−
<td align='center'>B</td>
+
              <td class="c16" colspan="1" rowspan="1">
−
<td align='center'>pDNA+RNA II short</td>
+
                <p class="c5"><span class="c0">scFv_antiVLY</span></p>
−
<td align='center'>0</td>
+
              </td>
−
</tr>
+
              <td class="c13" colspan="1" rowspan="1">
−
<tr>
+
                <p class="c5"><span class="c0">Single chain variable fragment antibody anti-vaginolysin (scFv_antiVGY)</span></p>
−
<td align='center'>RNAI</td>
+
              </td>
−
<td align='center'>RNA I</td>
+
              <td class="c7" colspan="1" rowspan="1">
−
<td align='center'>1E-6</td>
+
                <p class="c24"><span class="c0">733</span></p>
−
</tr>
+
              </td>
−
<tr>
+
          </tr>
−
<td align='center'>D</td>
+
          <tr class="c9">
−
<td align='center'>pDNA+RNA II long</td>
+
              <td class="c27" colspan="1" rowspan="1">
−
<td align='center'>0</td>
+
                <p class="c5"><span class="c0">BBa_K2622005</span></p>
−
</tr>
+
              </td>
−
<tr>
+
              <td class="c25" colspan="1" rowspan="1">
−
<td align='center'>E</td>
+
                <p class="c5"><span class="c0">Coding</span></p>
−
<td align='center'>pDNA+RNAII primer</td>
+
              </td>
−
<td align='center'>0</td>
+
              <td class="c16" colspan="1" rowspan="1">
−
</tr>
+
                <p class="c5"><span class="c0">GFP Nanobody</span></p>
−
<tr>
+
              </td>
−
<td align='center'>F</td>
+
              <td class="c13" colspan="1" rowspan="1">
−
<td align='center'>RNA II long</td>
+
                <p class="c5"><span class="c0">Camelid antibody against green fluorescent protein (GFP Nanobody)</span></p>
−
<td align='center'>0</td>
+
              </td>
−
</tr>
+
              <td class="c7" colspan="1" rowspan="1">
−
<tr>
+
                <p class="c24"><span class="c0">352</span></p>
−
<td align='center'>G</td>
+
              </td>
−
<td align='center'>pDNA</td>
+
          </tr>
−
<td align='center'>4E-8*</td>
+
          <tr class="c9">
−
</tr>
+
              <td class="c27" colspan="1" rowspan="1">
−
<tr>
+
                <p class="c5"><span class="c0">BBa_K2622006</span></p>
−
<td align='center'>H</td>
+
              </td>
−
<td align='center'>pDNA+RNA II+RNA I late</td>
+
              <td class="c25" colspan="1" rowspan="1">
−
<td align='center'>0</td>
+
                <p class="c5"><span class="c0">Coding</span></p>
−
</tr>
+
              </td>
−
<tr>
+
              <td class="c16" colspan="1" rowspan="1">
−
<td align='center'>RNA II</td>
+
                <p class="c5"><span class="c0">Mistic (Mstx)</span></p>
−
<td align='center'>RNA II</td>
+
              </td>
−
<td align='center'>0</td>
+
              <td class="c13" colspan="1" rowspan="1">
−
</tr>
+
                <p class="c5"><span>Membrane-integrating protein Mistic (MstX) from </span><span class="c22">Bacillus subtilis</span></p>
−
<tr>
+
              </td>
−
<td align='center'>J</td>
+
              <td class="c7" colspan="1" rowspan="1">
−
<td align='center'>RNAI+RNAII</td>
+
                <p class="c24"><span class="c0">330</span></p>
−
<td align='center'>0</td>
+
              </td>
−
</tr>
+
          </tr>
−
</tbody>
+
          <tr class="c9">
−
</table>
+
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622007</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">gRNA for ribosome protein L12</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Guide RNA for modification of ribosome protein L12 using CRISPR-Cas9 system</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">174</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622008</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">gRNA for ribosome protein L23</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Guide RNA for modification of ribosome protein L23 using CRISPR-Cas9 system</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">174</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622009</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">gRNA for ribosome protein L24</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Guide RNA for modification of ribosome protein L24 using CRISPR-Cas9 system</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">174</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622010</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch1 (GJ2)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 1</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">39</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622011</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch2 (GJ3)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 2</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">38</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622012</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch3 (GJ6)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 3</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">53</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622013</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch4 (GJ9)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 4</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">32</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622014</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch5 (GJ10)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 5</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">33</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622015</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Coding</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BamC</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Beta-barrel assembly protein C (BamC)</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">1057</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622016</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch 6 (Sw2)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 6</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">33</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622017</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch 7 (Sw3)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 7</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">33</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622018</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch 8 (Sw6)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 8</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">33</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622019</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch 9 (Sw7)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 9</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">33</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622020</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch 10 (Sw9)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 10</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">33</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622021</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Regulatory</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">RNA thermoswitch 11 (Sw11)</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Temperature-sensitive non-coding RNA 11</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">33</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622022</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Coding</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BamD</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Beta-barrel assembly protein D (BamD)</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">757</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622023</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Coding</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BamE</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Beta-barrel assembly protein E (BamE)</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">448</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622024</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Coding</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">SurA</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Survival protein A (SurA)</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">1374</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">BBa_K2622048</span></p>
 +
              </td>
 +
              <td class="c25" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Coding</span></p>
 +
              </td>
 +
              <td class="c16" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">OmpA full</span></p>
 +
              </td>
 +
              <td class="c13" colspan="1" rowspan="1">
 +
                <p class="c5"><span class="c0">Full length outer transmembrane protein A (OmpA)</span></p>
 +
              </td>
 +
              <td class="c7" colspan="1" rowspan="1">
 +
                <p class="c24"><span class="c0">1041</span></p>
 +
              </td>
 +
          </tr>
 +
        </tbody>
 +
    </table>
 
     </div>
 
     </div>
 
</div>
 
</div>

Revision as of 00:36, 18 October 2018

Basic Parts

Separate Soldiers

Basic parts, that we created fall into three categories: coding, regulatory and RNA. Coding parts consist of proteins, that assemble into lipid bilayer. Regulatory parts ensure that protein synthesis starts at desired temperature. RNA is used as a modification platform to produce membrane associated ribosomes which help with protein integration into membrane.

Basic Parts

Part Number

Type

Name

Brief description

Length, nt

BBa_K2622001

Coding

BamB

Beta-barrel assembly protein B (BamB)

1261

BBa_K2622002

Coding

BamA

Beta-barrel assembly protein A (BamA)

2520

BBa_K2622003

Coding

IgA

Immunoglobulin protease A (IgA) beta-domain from Neisseria gonorrhoeae

1398

BBa_K2622004

Coding

scFv_antiVLY

Single chain variable fragment antibody anti-vaginolysin (scFv_antiVGY)

733

BBa_K2622005

Coding

GFP Nanobody

Camelid antibody against green fluorescent protein (GFP Nanobody)

352

BBa_K2622006

Coding

Mistic (Mstx)

Membrane-integrating protein Mistic (MstX) from Bacillus subtilis

330

BBa_K2622007

RNA

gRNA for ribosome protein L12

Guide RNA for modification of ribosome protein L12 using CRISPR-Cas9 system

174

BBa_K2622008

RNA

gRNA for ribosome protein L23

Guide RNA for modification of ribosome protein L23 using CRISPR-Cas9 system

174

BBa_K2622009

RNA

gRNA for ribosome protein L24

Guide RNA for modification of ribosome protein L24 using CRISPR-Cas9 system

174

BBa_K2622010

Regulatory

RNA thermoswitch1 (GJ2)

Temperature-sensitive non-coding RNA 1

39

BBa_K2622011

Regulatory

RNA thermoswitch2 (GJ3)

Temperature-sensitive non-coding RNA 2

38

BBa_K2622012

Regulatory

RNA thermoswitch3 (GJ6)

Temperature-sensitive non-coding RNA 3

53

BBa_K2622013

Regulatory

RNA thermoswitch4 (GJ9)

Temperature-sensitive non-coding RNA 4

32

BBa_K2622014

Regulatory

RNA thermoswitch5 (GJ10)

Temperature-sensitive non-coding RNA 5

33

BBa_K2622015

Coding

BamC

Beta-barrel assembly protein C (BamC)

1057

BBa_K2622016

Regulatory

RNA thermoswitch 6 (Sw2)

Temperature-sensitive non-coding RNA 6

33

BBa_K2622017

Regulatory

RNA thermoswitch 7 (Sw3)

Temperature-sensitive non-coding RNA 7

33

BBa_K2622018

Regulatory

RNA thermoswitch 8 (Sw6)

Temperature-sensitive non-coding RNA 8

33

BBa_K2622019

Regulatory

RNA thermoswitch 9 (Sw7)

Temperature-sensitive non-coding RNA 9

33

BBa_K2622020

Regulatory

RNA thermoswitch 10 (Sw9)

Temperature-sensitive non-coding RNA 10

33

BBa_K2622021

Regulatory

RNA thermoswitch 11 (Sw11)

Temperature-sensitive non-coding RNA 11

33

BBa_K2622022

Coding

BamD

Beta-barrel assembly protein D (BamD)

757

BBa_K2622023

Coding

BamE

Beta-barrel assembly protein E (BamE)

448

BBa_K2622024

Coding

SurA

Survival protein A (SurA)

1374

BBa_K2622048

Coding

OmpA full

Full length outer transmembrane protein A (OmpA)

1041
invert