Difference between revisions of "Team:DLUT China B/Applied Design"

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     <title>Applied Design</title>
 
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         <h2 class="title">Overview</h2>
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            <p>We design a visualized liquid-crystal-based urine protein detector for early diagnosis of nephropathy.
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                The antibody molecules are bound by antigens immobilized on the substrate of the liquid crystal cell,
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                causing changes in the alignment of the liquid crystal molecules. After the polarized light passes,
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                    <p>Dr. Familict, the visualized liquid-crystal-based urine protein detector, is a 20cmx20cmx10cm brick-sized box. It consists mainly a slide holder slot, a wash buffer chamber, a waste collector, a liquid-crystal cell, polarizer and analyzer. Up on using, just plug in a antigen-coated glass slide to the slot, a drop of antibody solution is loaded automatically when the slide is placed properly. The user then only needs to add a drop of urine to the test zone, and press the bottom to start timing. The following operations will be done automatically. After the reaction is completed, the slide is rinsed for 30 s. Then it is drove slightly to the detection area. Upon slide positioning, liquid-crystal is loaded and the bottom light is switched on upon slide positioning. After the polarized light passes, visible color changes are produced. Users can take a picture and upload the result to the Mobile APP and get medication advice.</p>
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                different brightness and color changes are produced depending on the amount of the immobilized antibody.
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                 This product uses the sensitive marker of diabetic nephropathy, beta2 microglobulin, as an antigen to
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                ensure the detection of impaired renal function in the incipientDN. The detector is very simple to use.
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                The user only needs to add one drop of urine to the detector and obtain visible results. Thus, our
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                product is painless and household portable.</p>
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             <p>Our new application is to detect multiple proteins at the same time, and only need to add samples and antibody solutions to complete a simple visual inspection. We will develop a test kit for other diseases, the same principle is just to change to different markers and their antibodies. Therefore, our products have considerable application prospects.</p>
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                    <p>The liquid crystal cell is the most important part of the test box. When the upper slider slides to the observation area, it is assembled with the upper slide of the liquid crystal cell into a self-assembled film spaced 6 micrometers apart, and then the liquid crystal cell on the left side of the slide is opened, and the liquid crystal enters between the upper and lower slides due to capillary action. At this point, the microstructure of the slide will affect the orientation change of the liquid crystal. After turning on the light at the bottom of the test box, the light passes through the polarizer, then through the cell, and finally through the analyzer above the cell, so we can observe changes in brightness and color.</p>
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                    <p>These two components are used to wash unfixed antigens, antibodies and other impurity proteins on the slide. When the user drops the urine and the antibody solution, the test box starts to timing. After the reaction reaches 40 minutes, the time is over, the buffer chamber gate is opened, the slide is started to be washed for 30 seconds, and the buffer flows into the waste collector. When the waste collection area is full and can be replaced, as the same user can easily replace the buffer box by himself.</p>
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Revision as of 00:23, 18 October 2018

Applied Design

Applied Design

Overview

We design a visualized liquid-crystal-based urine protein detector for early diagnosis of nephropathy. The antibody molecules are bound by antigens immobilized on the substrate of the liquid crystal cell, causing changes in the alignment of the liquid crystal molecules. After the polarized light passes, different brightness and color changes are produced depending on the amount of the immobilized antibody. This product uses the sensitive marker of diabetic nephropathy, beta2 microglobulin, as an antigen to ensure the detection of impaired renal function in the incipientDN. The detector is very simple to use. The user only needs to add one drop of urine to the detector and obtain visible results. Thus, our product is painless and household portable.

Our new application is to detect multiple proteins at the same time, and only need to add samples and antibody solutions to complete a simple visual inspection. We will develop a test kit for other diseases, the same principle is just to change to different markers and their antibodies. Therefore, our products have considerable application prospects.