Difference between revisions of "Team:TecCEM/Measurement"

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                         <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/e/ea/T--TecCEM---sof6.png"
 
                         <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/e/ea/T--TecCEM---sof6.png"
 
                             alt="cell selection cellcount">
 
                             alt="cell selection cellcount">
                         <figcaption class="figure-caption text-xs-right">Figure 6. Cell Selection Cell Count
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                         <figcaption class="figure-caption text-xs-right">Figure 1. Cell Selection Cell Count
 
                         </figcaption>
 
                         </figcaption>
 
                     </figure>
 
                     </figure>
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                                 <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/4/46/T--TecCEM---sof7.png"
 
                                 <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/4/46/T--TecCEM---sof7.png"
 
                                     alt="result 1 cellcount">
 
                                     alt="result 1 cellcount">
                                 <figcaption class="figure-caption text-xs-right">Figure 7. Result of First Test - Cell Count
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                                 <figcaption class="figure-caption text-xs-right">Figure 2. Result of First Test - Cell Count
 
                                 </figcaption>
 
                                 </figcaption>
 
                             </figure>
 
                             </figure>
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                                 <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/0/0a/T--TecCEM---sof8.png"
 
                                 <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/0/0a/T--TecCEM---sof8.png"
 
                                     alt="result 2 cellcount">
 
                                     alt="result 2 cellcount">
                                 <figcaption class="figure-caption text-xs-right">Figure 8. Result of Second Test - Cell Count
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                                 <figcaption class="figure-caption text-xs-right">Figure 3. Result of Second Test - Cell Count
 
                                 </figcaption>
 
                                 </figcaption>
 
                             </figure>
 
                             </figure>
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                         <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/b/bb/T--TecCEM---sof9.jpg"
 
                         <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/b/bb/T--TecCEM---sof9.jpg"
 
                             alt="process cellcount">
 
                             alt="process cellcount">
                         <figcaption class="figure-caption text-xs-right">Figure 9. Image to be processed by Cell Count
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                         <figcaption class="figure-caption text-xs-right">Figure 4. Image to be processed by Cell Count
 
                         </figcaption>
 
                         </figcaption>
 
                     </figure>
 
                     </figure>
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                         <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/8/8d/T--TecCEM---sof10.png"
 
                         <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/8/8d/T--TecCEM---sof10.png"
 
                             alt="result after cellcount">
 
                             alt="result after cellcount">
                         <figcaption class="figure-caption text-xs-right">Figure 10. Image processeed by our software - Second cell type
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                         <figcaption class="figure-caption text-xs-right">Figure 5. Image processeed by our software - Second cell type
 
                         </figcaption>
 
                         </figcaption>
 
                     </figure>
 
                     </figure>
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                                 <img class="figure-img img-fluid text-center rounded" src="https://static.igem.org/mediawiki/2018/1/19/T--TecCEM---sof11.png"
 
                                 <img class="figure-img img-fluid text-center rounded" src="https://static.igem.org/mediawiki/2018/1/19/T--TecCEM---sof11.png"
 
                                     alt="numeric approximation result  after cellcount">
 
                                     alt="numeric approximation result  after cellcount">
                                 <figcaption class="figure-caption text-xs-center">Figure 11. Approximation of cell count
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                                 <figcaption class="figure-caption text-xs-center">Figure 6. Approximation of cell count
 
                                 </figcaption>
 
                                 </figcaption>
 
                             </figure>
 
                             </figure>
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                                 <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/c/c5/T--TecCEM---sof12.png"
 
                                 <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/c/c5/T--TecCEM---sof12.png"
 
                                     alt="second test result after cellcount">
 
                                     alt="second test result after cellcount">
                                 <figcaption class="figure-caption text-xs-right">Figure 12. Image processeed by our software - First cell type
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                                 <figcaption class="figure-caption text-xs-right">Figure 7. Image processeed by our software - First cell type
 
                                 </figcaption>
 
                                 </figcaption>
 
                             </figure>
 
                             </figure>
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                                 <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/6/6f/T--TecCEM---sof13.png"
 
                                 <img class="figure-img img-fluid rounded" src="https://static.igem.org/mediawiki/2018/6/6f/T--TecCEM---sof13.png"
 
                                     alt="second numeric approximation result  after cellcount">
 
                                     alt="second numeric approximation result  after cellcount">
                                 <figcaption class="figure-caption text-xs-right">Figure 13. Second numeric approximation
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                                 <figcaption class="figure-caption text-xs-right">Figure 8. Second numeric approximation
 
                                 </figcaption>
 
                                 </figcaption>
 
                             </figure>
 
                             </figure>

Revision as of 00:33, 18 October 2018

Cell Gif

Measurement

One of the crucial parts in cell culture is the determination of confluency, but with the current techniques this process is slow and imprecise. That is why we developed a software using machine learning to analyze microscope images tha throw the percentage of cell confluence. The importance of having a correctly cell confluency measurement is to avoid cell apoptosis because of the reduced space in the cases of a 80 to 100% of confluency. So, having a software that can give you a more precise result of the cell proliferation that is important to maintain your cell culture in good conditions without contamination and an excessive proliferation in it that would induce cell dead. The manipulation of this software is easy to use for everybody because is carefully realized and automatized so its implementation has a friendly interface. This software represents a revolution in the way cell culture is managed

Cell Count

The cell count software is a beta version image processing program that takes an input image and outputs a close estimate of the number of cells in the picture. The main reasons of it to still be a beta is because we would like to return the confluence percentage, as well as perform more tests on the software.

This software works for two kinds of cell colonies. To use it, it is necessary to access the web application and go to the section of Cell Count found on the top left hand. Upload your picture and wait for the results.

cell selection cellcount
Figure 1. Cell Selection Cell Count
result 1 cellcount
Figure 2. Result of First Test - Cell Count
result 2 cellcount
Figure 3. Result of Second Test - Cell Count

The cell counting algorithm works as follows:


process cellcount
Figure 4. Image to be processed by Cell Count
result after cellcount
Figure 5. Image processeed by our software - Second cell type
numeric approximation result  after cellcount
Figure 6. Approximation of cell count

In this example, we got an approximation of 990 cells counted.

second test result after cellcount
Figure 7. Image processeed by our software - First cell type
second numeric approximation result  after cellcount
Figure 8. Second numeric approximation

This software is planned to be a relief for a lot of researchers that make this process the hard way. This will optimize time, effort and resources letting the scientists do more science and less counting.