Difference between revisions of "Team:ECUST/Biocide"
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<p>When the concentration of Fe<sup>3+</sup> in the environment is high, the hard skeleton of the microenvironment of the <i>iron bacteria</i> will be destroyed and lose its protection. At this time, the added bactericide will have a greater killing effect on the bacteria. The bactericide we selected was cecropin AD, a cationic chimeric antimicrobial peptide obtained from cecropin, consisting of the first 11 amino acid residues of cecropin A and the last 26 amino acid residues of cecropin D. It has strong antibacterial activity against Gram-positive and negative bacteria.</p> | <p>When the concentration of Fe<sup>3+</sup> in the environment is high, the hard skeleton of the microenvironment of the <i>iron bacteria</i> will be destroyed and lose its protection. At this time, the added bactericide will have a greater killing effect on the bacteria. The bactericide we selected was cecropin AD, a cationic chimeric antimicrobial peptide obtained from cecropin, consisting of the first 11 amino acid residues of cecropin A and the last 26 amino acid residues of cecropin D. It has strong antibacterial activity against Gram-positive and negative bacteria.</p> | ||
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Revision as of 02:13, 18 October 2018
Description
When the concentration of Fe3+ in the environment is high, the hard skeleton of the microenvironment of the iron bacteria will be destroyed and lose its protection. At this time, the added bactericide will have a greater killing effect on the bacteria. The bactericide we selected was cecropin AD, a cationic chimeric antimicrobial peptide obtained from cecropin, consisting of the first 11 amino acid residues of cecropin A and the last 26 amino acid residues of cecropin D. It has strong antibacterial activity against Gram-positive and negative bacteria.
Design
Result
We insert fragment of Cecropin AD into vector pET-28a(Figure 1)
The plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR(Figure 2).
We verified the expression of Cecropin AD by SDS-PAGE(Figure 3).
To test the effect of cecropin AD, we measured the growth curve of iron bacteria adding with different concentration of cecropin AD.
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