Difference between revisions of "Team:Vilnius-Lithuania/LabBook"

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         <h1>Lab Book</h1>
 
         <h1>Lab Book</h1>
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             <p>It appears you don't have a PDF plugin for this browser, you can <a href="https://static.igem.org/mediawiki/2018/8/81/T--Vilnius-Lithuania--Week_1_Labook.pdf
 
                 ">click here to
 
                 ">click here to
 
               download the PDF file.</a>
 
               download the PDF file.</a>
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                    ">click here to
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        <p>The framework also includes a possibility of adding a selection system that reduces the usage of antibiotics
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                  download the PDF file.</a>
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            (only 1 antibiotic for up to 5 different plasmids!) and an active partitioning system to make sure that low
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            copy number plasmid groups are not lost during the division.
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        <h2>Applications</h2>
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            <h5>Everyday lab work</h5>
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                A multi-plasmid system that is easy to assemble and control. With our framework the need to limit your
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                research to a particular plasmid copy number just because there are not enough right replicons to
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                choose from, is eliminated. With SynORI you can easily create a vector with a desired copy number that
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                suits your needs.</li>
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            <h5>Biological computing</h5>
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                The ability to choose a wide range of copy number options and their control types will make the
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                synthetic biology engineering much more flexible and predictable. Introduction of plasmid copy number
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                regulation is equivalent to adding a global parameter to a computer system. It enables the coordination
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                of multiple gene group expression.
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            <h5>Smart assembly of large protein complexes</h5>
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                The co-expression of multi-subunit complexes using different replicons brings incoherency to an already
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                chaotic cell system. This can be avoided by using SynORI, as in this framework every plasmid group uses
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                the same type of control, and in addition can act in a group-specific manner.</p>
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            <h5>Metabolic engineering</h5>
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                A big challenge for heterologous expression of multiple gene pathways is to accurately adjust the
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                levels of each enzyme to achieve optimal production efficiency. Precise promoter tuning in
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                transcriptional control and synthetic ribosome binding sites in translational control are already
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                widely used to maintain expression levels. In addition to current approaches, our framework allows a
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                simultaneous multiple gene control. Furthermore, an inducible regulation that we offer, can make the
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                search for perfect conditions a lot easier.
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Revision as of 02:41, 18 October 2018

Lab Book

Writing a Book

Our team intensively and precisely worked in the lab for 15 weeks from the beginning of summer till the middle of autumn to develop a SynDrop idea. Here is a detailed documentation of our Wet Lab work, including tasks, reagents used and conditions needed during the experiments.

Lab Book

It appears you don't have a PDF plugin for this browser, you can click here to download the PDF file.

It appears you don't have a PDF plugin for this browser, you can click here to download the PDF file.

Species sign in ODE system Species Initial concentration (M)
A pDNA+RNA I+RNAII early 0
B pDNA+RNA II short 0
RNAI RNA I 1E-6
D pDNA+RNA II long 0
E pDNA+RNAII primer 0
F RNA II long 0
G pDNA 4E-8*
H pDNA+RNA II+RNA I late 0
RNA II RNA II 0
J RNAI+RNAII 0
invert