$("#Content3").html("<img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/7/77/T--Warwick--ribodesign6.png'><br><p>The Gamma construct was designed by analysing the secondary structure of the Legiolysin mRNA and locating exposed nucleotides. The potential loop recognition domain highlighted in red shows the region that construct Gamma was designed through prediction of exposed nucleotides for the optimal mRNA-gRNA interaction efficiency. We utilised extensive secondary structure prediction through our model utilising NuPack to determine functional switch sequences.</p><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/3/33/T--Warwick--ribodesign7.png'><br><p>The <a href='http://parts.igem.org/Part:BBa_K2841541'>Alpha construct</a> prediction determined that the Blocking domain effectively obstructed the Targeting Domain. The blocked targeting domain would not function, thus the switch would be in the off state.</p><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/9/94/T--Warwick--ribodesign8.png'><br><p>The Alpha and Pathogen Imitating Sequence complex prediction determined that the Blocking domain was not able to be removed from the Targeting Domain, implying that the switch would remain in the off state regardless of stimulation. Despite the information we experimented with the construct to test the accuracy of the modelling software and found that the model was not representative of results. A large amount of our time was dedicated to <a href='https://2018.igem.org/Team:Warwick/Model'>modelling</a> our constructs until they demonstrated effective function.</p><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/a/aa/T--Warwick--2018-DNAwhole.gif'><br><p>We used centrifold method of 3D RNA folding prediction with RNA composer to test the tertiary structure of our constructs with the 2D predicted structures from NuPack. <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703931/'>The predicted structures</a> supported our initial conclusions that the targeting domain of the gRNA (green) was inhibited by the blocking domain (red) and that the sensing loop (blue) and extended sensing domain (light blue) could unfold the structure while the dCAS9 recognition cis acting factor (grey) did not disrupt the blocking and targeting domains. Watson-Crick base pairing of the gRNA targeting domain and the blocking domain is clear through analysis of the molecular structure. <br>We used centrifold method of folding with RNA composer to get some <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703931/'>results</a></p>");

         $("#Content3").html("<img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/7/77/T--Warwick--ribodesign6.png'><br><p>The Gamma construct was designed by analysing the secondary structure of the Legiolysin mRNA and locating exposed nucleotides. The potential loop recognition domain highlighted in red shows the region that construct Gamma was designed through prediction of exposed nucleotides for the optimal mRNA-gRNA interaction efficiency. We utilised extensive secondary structure prediction through our model utilising NuPack to determine functional switch sequences.</p><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/3/33/T--Warwick--ribodesign7.png'><br><p>The <a href='http://parts.igem.org/Part:BBa_K2841541'>Alpha construct</a> prediction determined that the Blocking domain effectively obstructed the Targeting Domain. The blocked targeting domain would not function, thus the switch would be in the off state.</p><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/9/94/T--Warwick--ribodesign8.png'><br><p>The Alpha and Pathogen Imitating Sequence complex prediction determined that the Blocking domain was not able to be removed from the Targeting Domain, implying that the switch would remain in the off state regardless of stimulation. Despite the information we experimented with the construct to test the accuracy of the modelling software and found that the model was not representative of results. A large amount of our time was dedicated to <a href='https://2018.igem.org/Team:Warwick/Model'>modelling</a> our constructs until they demonstrated effective function.</p><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/a/aa/T--Warwick--2018-DNAwhole.gif'><br><p>We used centrifold method of 3D RNA folding prediction with RNA composer to test the tertiary structure of our constructs with the 2D predicted structures from NuPack. <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703931/'>The predicted structures</a> supported our initial conclusions that the targeting domain of the gRNA (green) was inhibited by the blocking domain (red) and that the sensing loop (blue) and extended sensing domain (light blue) could unfold the structure while the dCAS9 recognition cis acting factor (grey) did not disrupt the blocking and targeting domains. Watson-Crick base pairing of the gRNA targeting domain and the blocking domain is clear through analysis of the molecular structure. <br>We used centrifold method of folding with RNA composer to get some <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703931/'>results</a></p>");

}

}