Part:BBa_K2549021 αCD19-mN1c-tTAA

Introduction

This year we have provided 7 versions of SynNotch receptors in our BioBrick submission, enabling others to wire their contact-dependent signal transduction in mammalian cells. Multiple combinations of extracellular domains, transmembrane core regions and intracellular domains make it even easier for others to readily assemble their own desirable genetic circuits.

Among the 7 SynNotch receptors, our favorite one is αCD19-mN1c-tTAA (BBa_K2549021)

How αCD19-mN1c-tTAA works

It receives ligand-dependent signal via the CD19 scFv and undergoes a cleavage process in which the tTA advance is released, then entering into the nucleus to activate the expression of TRE3GV promotor. Thus it can be served as a signal input module.

Advantages of αCD19-mN1c-tTAA

We have conducted flow cytometry experiments to test our SynNotch receptors and after testing, αCD19-mN1c-tTAA have stood out for showing the highest signal-to-noise ratio. We have also discovered that it has the highest activation ratio when activated by surface-expressed CD19 antigen. Moreover, it also shows only a few amount of false activation which can be tolerated. As it performs great modularity and has a great potential to be utilized by others to assemble their own CD19-dependent signal transduction module, this especially enables the possibility of the clinical application of SynNotch receptors.

Figure 1. Flow cytometry characterization of SynNotch receptors. TRE3GV-EGFP circuit was set to indicate the activation level of SynNotch receptors. It is shown that αCD19-mN1c-tTAA has the highest signal-to-noise ratio.

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