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{{RHIT}} | {{RHIT}} | ||
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<h1>Genetics Model </h1> | <h1>Genetics Model </h1> | ||
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<p>The DNA coding for the 6 enzymes required for breakdown and assimilation of PET was too long to fit on one plasmid. To rectify this and to be able to test smaller subsystems, the PETase and MHETase genes were placed on Backbone 1, Plasmid 1. The Glycolaldehyde Reductase, Glycolaldehyde Dehydrogenase, and Glycolate Oxidase, and Malate Synthase were placed in sequence on Backbone 2, Plasmid 2.</p> | <p>The DNA coding for the 6 enzymes required for breakdown and assimilation of PET was too long to fit on one plasmid. To rectify this and to be able to test smaller subsystems, the PETase and MHETase genes were placed on Backbone 1, Plasmid 1. The Glycolaldehyde Reductase, Glycolaldehyde Dehydrogenase, and Glycolate Oxidase, and Malate Synthase were placed in sequence on Backbone 2, Plasmid 2.</p> | ||
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<h1>Plasmid 1</h1> | <h1>Plasmid 1</h1> | ||
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<div class="container"> | <div class="container"> | ||
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<img src="https://static.igem.org/mediawiki/2018/5/53/T--RHIT--Plasmid1Parameters1.png"> | <img src="https://static.igem.org/mediawiki/2018/5/53/T--RHIT--Plasmid1Parameters1.png"> | ||
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</center> | </center> | ||
<p> <a href = "https://bmcsystbiol.biomedcentral.com/articles/10.1186/1752-0509-5-111"> | <p> <a href = "https://bmcsystbiol.biomedcentral.com/articles/10.1186/1752-0509-5-111"> | ||
− | https://bmcsystbiol.biomedcentral.com/articles/10.1186/1752-0509-5-111 </a> useful description and numbers for AraC promoter</p> </ | + | https://bmcsystbiol.biomedcentral.com/articles/10.1186/1752-0509-5-111 </a> useful description and numbers for AraC promoter</p> |
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<div class = "clear extra_space"></div> | <div class = "clear extra_space"></div> | ||
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</div></div> | </div></div> | ||
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<img src="https://static.igem.org/mediawiki/2018/d/d0/T--RHIT--Plasmid2Parameters1.png"> | <img src="https://static.igem.org/mediawiki/2018/d/d0/T--RHIT--Plasmid2Parameters1.png"> | ||
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<img src="https://static.igem.org/mediawiki/2018/b/bb/T--RHIT--Plasmid2EqNew1.png"> | <img src="https://static.igem.org/mediawiki/2018/b/bb/T--RHIT--Plasmid2EqNew1.png"> | ||
</center> | </center> | ||
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Revision as of 16:33, 9 July 2018