Difference between revisions of "Team:FSU/Description"

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                                 <h2 class="md-title" style="text-align: center;">Project Description</h2>
 
                                 <h2 class="md-title" style="text-align: center;">Project Description</h2>
 
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                                     <p><b>Question</b><br> Can sound be used to induce <b>gene expression</b> in engineered E. coli?</p>  
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                                     <p><b>Question</b><br> Can sound be used to induce gene expression in engineered E. coli?</p>  
 
                                     <p><b>Approach</b><br> Today, it is routine to induce the expression of a gene in E. coli using a small molecule.  Examples are arabinose, lactose, and rhamnose.  Can sound become a routine means to induce gene expression in engineered E. coli?  Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question but in mammalian cells.  We are studying the details of both projects.  We are characterizing parts submitted by the 2008 UC Berkeley team that are putative promoters at the end of a signal transduction pathway that responds to sound.  In parallel, we are studying genes that, according to published scientific reports, are up-regulated when bacteria are exposed to sound.  We will identify promoters that seem to be induced by pathways that respond to sound.  The selected promoters will be used to design new genetic devices that will generate chromogenic or fluorescent reporters when a cell is exposed to sounds.  We will use the new genetic devices to correlate sound frequencies, amplitudes, and exposure times with levels of gene expression.</p>
 
                                     <p><b>Approach</b><br> Today, it is routine to induce the expression of a gene in E. coli using a small molecule.  Examples are arabinose, lactose, and rhamnose.  Can sound become a routine means to induce gene expression in engineered E. coli?  Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question but in mammalian cells.  We are studying the details of both projects.  We are characterizing parts submitted by the 2008 UC Berkeley team that are putative promoters at the end of a signal transduction pathway that responds to sound.  In parallel, we are studying genes that, according to published scientific reports, are up-regulated when bacteria are exposed to sound.  We will identify promoters that seem to be induced by pathways that respond to sound.  The selected promoters will be used to design new genetic devices that will generate chromogenic or fluorescent reporters when a cell is exposed to sounds.  We will use the new genetic devices to correlate sound frequencies, amplitudes, and exposure times with levels of gene expression.</p>
 
                                   <p><b>Impact</b><br> If we are successful in using sound to induce gene express in E. coli, the impact is unclear.  Our Human Practices team has started discussions with molecular biologists, brewmasters, and managers of water treatment systems to explore the potential positive and negative impacts of using sound to induce gene expression.  We will also have design thinking workshops focused on exploring the potential use and misuse of the technology.</p>
 
                                   <p><b>Impact</b><br> If we are successful in using sound to induce gene express in E. coli, the impact is unclear.  Our Human Practices team has started discussions with molecular biologists, brewmasters, and managers of water treatment systems to explore the potential positive and negative impacts of using sound to induce gene expression.  We will also have design thinking workshops focused on exploring the potential use and misuse of the technology.</p>

Revision as of 20:25, 6 July 2018

Project Description

Question
Can sound be used to induce gene expression in engineered E. coli?

Approach
Today, it is routine to induce the expression of a gene in E. coli using a small molecule. Examples are arabinose, lactose, and rhamnose. Can sound become a routine means to induce gene expression in engineered E. coli? Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question but in mammalian cells. We are studying the details of both projects. We are characterizing parts submitted by the 2008 UC Berkeley team that are putative promoters at the end of a signal transduction pathway that responds to sound. In parallel, we are studying genes that, according to published scientific reports, are up-regulated when bacteria are exposed to sound. We will identify promoters that seem to be induced by pathways that respond to sound. The selected promoters will be used to design new genetic devices that will generate chromogenic or fluorescent reporters when a cell is exposed to sounds. We will use the new genetic devices to correlate sound frequencies, amplitudes, and exposure times with levels of gene expression.

Impact
If we are successful in using sound to induce gene express in E. coli, the impact is unclear. Our Human Practices team has started discussions with molecular biologists, brewmasters, and managers of water treatment systems to explore the potential positive and negative impacts of using sound to induce gene expression. We will also have design thinking workshops focused on exploring the potential use and misuse of the technology.

Description

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