Difference between revisions of "Team:NTNU Trondheim/Notebook"

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             <div class="collapsible-body"><span> <b>Goal:</b> Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.  
 
             <div class="collapsible-body"><span> <b>Goal:</b> Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.  
  
−
<br> <b>Preparation of LA- medium </b> <br>
+
<br> <b> 1. Preparation of LA- medium </b> <br>
 
LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C.
 
LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C.
  
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<li>5g        NaCl</li>
 
<li>5g        NaCl</li>
  
−
<li>15g      Agar </li></span></div>
+
<li>15g      Agar </li>
 +
 
 +
<br>
 +
 
 +
<b>2. Agar plates with antibiotics </b> <br>
 +
 
 +
Two different agar plates were made – one type contains ampicillin (AMP) while the other type contains chloramphenicol (CM), and were prepared after the following recipe:
 +
<br>
 +
LA- medium with AMP:
 +
 
 +
<ul>
 +
 
 +
<li>0,5L    LA-medium </li>
 +
 
 +
<li>0,5mL AMP (50mg/mL)</li>
 +
</ul>
 +
<br>
 +
LA-medium with CM:
 +
<ul>
 +
<li>0,5L    LA-medium</li>
 +
 
 +
<li>0,5mL CM (35mg/mL)</li>
 +
</ul>
 +
 
 +
The LA-media with antibiotic were poured on petri dishes.
 +
 
 +
</span></div>
 
           </li>
 
           </li>
 
             <li>
 
             <li>

Revision as of 12:18, 9 July 2018

Notebook


  • Week
    25

    Note title

    • Jun
      18

      Test

      Test.
    • Jun
      19

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      20

      Our first day in the laboratory

      Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.
      1. Preparation of LA- medium
      LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C.
      • 1L Distilled water
      • 5g Yeast extract
      • 10g Tryptone
      • 5g NaCl
      • 15g Agar

        • 2. Agar plates with antibiotics
        Two different agar plates were made – one type contains ampicillin (AMP) while the other type contains chloramphenicol (CM), and were prepared after the following recipe:
        LA- medium with AMP:
        • 0,5L LA-medium
        • 0,5mL AMP (50mg/mL)

        LA-medium with CM:
        • 0,5L LA-medium
        • 0,5mL CM (35mg/mL)
        The LA-media with antibiotic were poured on petri dishes.
    • Jun
      21

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      22

      Isolation and verification of pgRNA and pdCas9

      Test
  • Week
    26

    Note title

    • Jun
      25

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      26

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      27

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      28

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      29

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    27

    Note title

    • Jul
      1

      Note title

    • Jul
      2

      InterLabs Study

      Calibration 1 and 2 for the InterLabs Study.
    • Jul
      3

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      4

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      5

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    28

    Note title

    • Jul
      9

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      10

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      11

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      12

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      13

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    29

    Note title

    • Jul
      16

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      17

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      18

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      19

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      20

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    30

    Note title

    • Jul
      23

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      24

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      25

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      26

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      27

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    31

    Note title

    • Jul
      30

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      31

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      1

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      2

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      3

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    32

    Note title

    • Aug
      6

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      7

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      8

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      9

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      10

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    33

    Note title

    • Aug
      13

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      14

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      15

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      16

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      17

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    34

    Note title

    • Aug
      20

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      21

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      22

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      23

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      24

      Note title

      Lorem ipsum dolor sit amet.