Revision as of 14:49, 12 July 2018

Notebook

Week

25

Getting started
- Jun
  
  18
  
  No lab
  
  Test.
- Jun
  
  19
  
  No lab
  
  Contacted some potential sponsors and continued our search for new ones. The project description was entered on our wiki page. We had a quick lab tour guided by our instructor Nikolay.
- Jun
  
  20
  
  Our first day in the laboratory!
  
  Contacted some sponsors, read articles related to our project and started on the script for the modelling of our project.
  We finally started working in the laboratory. We made LA-medium and plated bacteria carrying pgRNA and pdCas9 on agar plates with ampicillin and chloramphenicol, respectively. More details about the lab procedures throughout the project are given in our lab journal.
- Jun
  
  21
  
  Inoculation of E.coli
  
  Pure cultures were made by picking and inoculating colonies grown overnight on agar plates with antibiotics as selection agents.
- Jun
  
  22
  
  Isolation and verification of pgRNA and pdCas9
  
  Verified that the bacteria in our pure cultures had been correctly transformed with the pgRNA and the pdCas9. This was done by plasmid isolation (Miniprep), restriction cutting with appropriate enzymes and agarose gel electrophoresis (test digest).
Week

26

Note title
- Jun
  
  25
  
  Note title
  
  Inserted pgRNA and pdCas9 into competent E. coli DH5α by heat shock transformation, and aseptically spread plated the transformation mixes on separate agar plates with ampicillin and chloramphenicol, respectively. We also started preparing for the InterLabs Study.
- Jun
  
  26
  
  Note title
  
  Pure cultures were made by Inoculating colonies from the agar plates prepared yesterday. We started to work with the InterLab Study: Calibration curve 1 and 2 were made.
- Jun
  
  27
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jun
  
  28
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jun
  
  29
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

27

Note title
- Jul
  
  1
  
  Note title
- Jul
  
  2
  
  InterLabs Study
  
  Calibration 1 and 2 for the InterLabs Study.
- Jul
  
  3
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  4
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  5
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

28

Note title
- Jul
  
  9
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  10
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  11
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  12
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  13
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

29

Note title
- Jul
  
  16
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  17
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  18
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  19
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  20
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

30

Note title
- Jul
  
  23
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  24
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  25
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  26
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  27
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

31

Note title
- Jul
  
  30
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  31
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  1
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  2
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  3
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

32

Note title
- Aug
  
  6
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  7
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  8
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  9
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  10
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

33

Note title
- Aug
  
  13
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  14
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  15
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  16
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  17
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

34

Note title
- Aug
  
  20
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  21
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  22
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  23
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  24
  
  Note title
  
  Lorem ipsum dolor sit amet.

@@ Line 172: / Line 172: @@
               </div>
               <p>Note title</p></div>
-              <div class="collapsible-body"><span>Lorem ipsum dolor sit amet.</span></div>
+              <div class="collapsible-body"><span>
+Inserted pgRNA and pdCas9 into competent E. coli DH5α by heat shock transformation, and aseptically spread plated the transformation mixes on separate agar plates with ampicillin and chloramphenicol, respectively. We also started preparing for the InterLabs Study.
+</span></div>
             </li>
              <li>
@@ Line 187: / Line 189: @@
               </div>
               <p>Note title</p></div>
-              <div class="collapsible-body"><span>Lorem ipsum dolor sit amet.</span></div>
+              <div class="collapsible-body"><span>
+Pure cultures were made by Inoculating colonies from the agar plates prepared yesterday. We started to work with the InterLab Study: Calibration curve 1 and 2 were made.
+</span></div>
             </li>
              <li>

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Revision as of 14:49, 12 July 2018

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