Difference between revisions of "Team:Marburg"

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<h2>Welcome on the Wiki of the 2018th iGEM Team Marburg: VIBRIGENS – Accelerating Synbio.</h2>
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<h2>Vibrigens - Accelerating Synbio:<br>Establishing <i>Vibrio natriegens</i> as the new chassis organism for synthetic biology</h2>
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In our project we work with the fastest growing organism known to date, <i>Vibrio natriegens</i>. This organism was described for the first time in 1962 but mostly forgotten in the biology community. In the last few years some scientists proposed <i>V. natriegens</i> as an alternative for the commonly used organism in all aspects of molecular biology, including biotechnological applications, <i>Escherichia coli</i>. Until now most common methods are not available in <i>Vibrio natriegens</i> thus preventing its widespread use. In our work we want to establish and precisely characterize these essential methods and show its power by producing high-value chemicals and pharmaceuticals.<p>
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Waiting for cells to grow is an enormous time sink for synthetic biologists. Cloning cycles with
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We believe that our work will be highly beneficial for the whole community of synthetic biologists as well as for biotech companies.<p>
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the current standard, <i>Escherichia coli</i>, typically take up to three days. In our project Vibrigens
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In contrast to <i>E. coli</i> which relies on the expensive carbohydrate glucose, <i>V. natriegens</i> is able to grow on inexpensive carbon sources such as sucrose. Therefore using <i>V. natriegens</i> does not only reduce the time required for the production it also grows in less expensive media and shows increased production rates.<p>
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- Accelerating Synbio, we established the tools to turn <i>Vibrio natriegens</i> into the next
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In addition to biotechnological applications, the fast growth of <i>V. natriegens</i> will be also beneficial for research labs thus cutting down the time and money needed for basic research and the development of pharmaceuticals.
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generation chassis for synthetic biology, ready to be used reliably. By taking advantage of its
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unbeaten doubling time of 7 minutes, we substantially reduced waiting time and made oneday-cloning a reality. We built and characterized a flexible golden-gate-based part collection,
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consisting of more than 100 parts, which enables the creation of complex pathways in a short
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amount of time. Our engineered <i>V. natriegens</i> strains VibriClone and VibriExpress are
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designed for cloning and protein expression applications, respectively. Moreover, we
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established the first synthetic metabolic pathway in this organism by producing the platform
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chemical 3-Hydroxypropionate and along the way developed an accelerated workflow for
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metabolic engineering.
 
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Revision as of 19:26, 18 September 2018

Vibrigens - Accelerating Synbio:
Establishing Vibrio natriegens as the new chassis organism for synthetic biology

Waiting for cells to grow is an enormous time sink for synthetic biologists. Cloning cycles with the current standard, Escherichia coli, typically take up to three days. In our project Vibrigens - Accelerating Synbio, we established the tools to turn Vibrio natriegens into the next generation chassis for synthetic biology, ready to be used reliably. By taking advantage of its unbeaten doubling time of 7 minutes, we substantially reduced waiting time and made oneday-cloning a reality. We built and characterized a flexible golden-gate-based part collection, consisting of more than 100 parts, which enables the creation of complex pathways in a short amount of time. Our engineered V. natriegens strains VibriClone and VibriExpress are designed for cloning and protein expression applications, respectively. Moreover, we established the first synthetic metabolic pathway in this organism by producing the platform chemical 3-Hydroxypropionate and along the way developed an accelerated workflow for metabolic engineering.