Difference between revisions of "Team:SSHS-Shenzhen"

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<!DOCTYPE html>
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<html class="nojs html css_verticalspacer" lang="en-US">
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<head>
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  <meta http-equiv="Content-type" content="text/html;charset=UTF-8"/>
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  <meta name="generator" content="2018.0.0.379"/>
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  <meta name="viewport" content="width=device-width, initial-scale=1.0"/>
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  <script type="text/javascript">
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  // Update the 'nojs'/'js' class on the html node
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document.documentElement.className =
 
<!DOCTYPE html>
 
<!DOCTYPE html>
 
<html class="nojs html css_verticalspacer" lang="en-US">
 
<html class="nojs html css_verticalspacer" lang="en-US">
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   <title>Home</title>
 
   <title>Home</title>
 
   <!-- CSS -->
 
   <!-- CSS -->
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  </style>
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  <!-- JS includes -->
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  <!--[if lt IE 9]>
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  <script>
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  /*
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HTML5 Shiv v3.6.2 | @afarkas @jdalton @jon_neal @rem | MIT/GPL2 Licensed
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*/
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        <img class="block" id="u272_img" src="https://static.igem.org/mediawiki/2018/3/3c/T--SSHS-Shenzhen--gao.png" alt="" width="186" height="186"/>
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        </div>
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        <a class="nonblock nontext MuseLinkActive clearfix grpelem" id="u101-4" href="index.html" data-sizePolicy="fixed" data-pintopage="page_fixedLeft"><!-- content --><p>Shenzhen Senior High School</p></a>
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        </div>
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        <div class="MenuItemContainer clearfix grpelem" id="u141"><!-- vertical box -->
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Phyllotreta striolata killers

Phyllotreta striolata is the most destructive insects worldwide. However, the present insect control strategies have certain limitations. We aim to develop a RNAi-based topical application approach. Ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNA silencing mechanism in the P. striolata and lead to its death. So far, we have designed the siRNAs for synthesis based on the sequences of the genes that are important for the development and survival of P. striolata. Currently we are rearing P. striolata to obtain enough the same age insects for feeding bioassays and gene silencing investigation. We will design corresponding small hairpin RNAs (shRNAs) and use E. coli expression system, or in vitro transcription system to produce these shRNAs in large scale.

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Phyllotreta striolata killers

Phyllotreta striolata is the most destructive insects worldwide. However, the present insect control strategies have certain limitations. We aim to develop a RNAi-based topical application approach. Ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNA silencing mechanism in the P. striolata and lead to its death. So far, we have designed the siRNAs for synthesis based on the sequences of the genes that are important for the development and survival of P. striolata. Currently we are rearing P. striolata to obtain enough the same age insects for feeding bioassays and gene silencing investigation. We will design corresponding small hairpin RNAs (shRNAs) and use E. coli expression system, or in vitro transcription system to produce these shRNAs in large scale.

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