Difference between revisions of "Team:Grenoble-Alpes/team"

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</head>
 
</head>
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<body>
 
<body>
−
  <section class="sable flex">  
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<div class="navi">
−
    <article class="col-30">  
+
  <nav width="90%">
−
<img alt="" src="">
+
    <ul>
−
  <h2 class="titre_article"> Lorem Ipsum </h2>
+
      <li class="coche">
−
            <p class="article_texte"> fIn in tyrannorum atque neque diffluentes .</p>
+
        <a href="https://2018.igem.org/Team:Grenoble-Alpes">HOME</a>
−
    <p class="boutton_centrer"><a href="actu1.html"><button>en savoir plus...</button></a><p>  
+
      </li>
−
    </article>
+
      <li>
−
  </section>
+
        <a href="https://2018.igem.org/Team:Grenoble-Alpes/team">TEAM</a>
 +
      </li>
 +
      <li>
 +
        <a href="https://2018.igem.org/Team:Grenoble-Alpes/project">PROJECT</a>
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      </li>
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      <li>
 +
      <a href="humanpractices.html">HUMAN PRACTICES</a>
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      </li>
 +
      <li>
 +
      <a href="entrepreneurship.html">ENTREPRENEURSHIP</a>
 +
      </li>
 +
      <li>
 +
      <a href="ressources.html">RESSOURCES</a>
 +
      </li>
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    </ul>
 +
 
 +
</nav>
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</div>
 +
 
 +
<table  align="center" width="100%" style="margin: 0px;border-style:hidden; position:fixed; z-index:-1">
 +
<tr><td align="center" >
 +
<H1>Welcome on the iGEM Grenoble-Alpes wiki !</H1></td></tr>
 +
<td align="center"><IMG width="30%" SRC="https://static.igem.org/mediawiki/2018/0/0d/T--Grenoble-Alpes--logo.png" ></td></tr>
 +
</table>
 +
<table  align="center" width="100%" style="margin:0px; border-style:hidden; position:relative; top:700px; z-index:1"><tr class="font">
 +
 
 +
<td valign="top" style="background-color:#CCC0C0"><H1 align="center" >
 +
<u>Project description</H1></u>
 +
<br>
 +
 
 +
<H2 style="text-indent: 45px;" align="justify">
 +
According to a report from the World Health Organization (WHO), in 2050, each year ten million people will die from pathogenic bacteria that acquired antibiotic resistance if nothing is done.  
 +
<br></H2>
 +
 
 +
<H2 style="text-indent: 45px;" align="justify">
 +
This is why it is absolutely necessary to find an alternative that can counterbalance this growing antibioresistance. A solution, which is already used in Eastern European countries such as Georgia, is phagotherapy.
 +
</H2>
 +
 
 +
<H2 style="text-indent: 45px;" align="justify">
 +
This medicine is based on bacteriophages (also nicknamed phages) which are little viruses (from 50 to 200 nm) that can specifically infect a bacterial strain and amplify in it, sometimes leading to the cell death (lytic phages).
 +
<br></H2>
 +
 
 +
<H2 align="justify">This year, the goal of our team is to develop a fully automated system capable of :
 +
<ul>
 +
<li>identifying a pathogenic bacteria</li>
 +
<li>detecting resistance markers </li>
 +
<li>select the right phages for a possible phagotherapy.</li>
 +
</ul>
 +
 
 +
<H2 style="text-indent: 45px;" align="justify">To do this, we will use a DNA probe : a plasmid with a fluorescent gene and a one-stranded fraction which is complementary to the sequence we want to detect. Our automated system will achieve detection from a patient saliva sample. The device will first lyse the bacteria using the phages we are testing and lysis buffer as control. Then, DNA will be extracted using magnetic beads coated with silica. The purified DNA will be digested with the right restriction enzymes to isolate our fraction of interest. The next step will be the hybridization of these fragments with our DNA probes leading to its circularization. Following this, a transformation of the plasmid will be performed into E. Coli competent cells to allow expression of the fluorescence gene and detection.
 +
<br></H2>
 +
 
 +
<H2 style="text-indent: 45px;" align="justify">
 +
Our system will be made so the practitioner only has to load samples, run the protocol and come to see the results a few hours later.<br></H2>
 +
 
 +
<H2 style="text-indent: 45px;" align="justify">For our proof of concept, we will work on two pathogenic bacterial species usually found in nosocomial infection cases due to their ability to develop antibioc resistance : <i>Pseudomonas Aeruginosa</i> and <i>Staphylococcus Aureus</i>. We will make two different probes for each : one for detection of the bacteria, which consists on the recognition of housekeeping genes, and the other for detection of a resistance marker (point mutations on a gene encoding the bacterial wall for example). The two sequences will be detected by fluorescence, using RFP (red fluorescent protein) for housekeeping genes and BFP (blue fluorescent protein) for resistance.
 +
</H2>
 +
 
 +
</td></tr>
 +
<tr><td align="center"><H1 align="center">Have a look at our video !</H1><br><video width="50%" controls>
 +
<source src="https://static.igem.org/mediawiki/2018/9/99/T--Grenoble-Alpes--presentationvideo.mp4" type="video/mp4">
 +
</video> </td></tr>
 +
<tr><td align="center" style="background-color:#CCC0C0"><H1>
 +
<u>CONTACT</u></H1><br>
 +
<H2>igem.grenoble.alpes@gmail.com</H2><br>
 +
<IMG width="30%" SRC="https://static.igem.org/mediawiki/2018/3/3d/T--Grenoble-Alpes--logosreseaux.png">
 +
</td></tr>
 +
<tr><td align="center">
 +
<H1><u>THEY HELPED US</u></H1><br>
 +
<IMG width="70%" SRC="https://static.igem.org/mediawiki/2018/6/67/T--Grenoble-Alpes--logospartenaires.png">
 +
</td></tr>
 
</body>
 
</body>
 +
 +
 +
 +
<!----
 +
<nav style="width:100%; padding:0px; margin:0px">
 +
<ul class="menu"><li class="coche"><a href="https://2018.igem.org/Team:Grenoble-Alpes/home.html">HOME</a>
 +
</li>
 +
<li><a href="team.html">TEAM</a>
 +
</li>
 +
<li><a href="project.html">PROJECT</a>
 +
</li>
 +
<li><a href="humanpractices.html">HUMAN PRACTICES</a>
 +
</li>
 +
<li><a href="entrepreneurship.html">ENTREPRENEURSHIP</a>
 +
</li>
 +
<li><a href="labbook.html">LABBOOK</a>
 +
</li>
 +
</ul>
 +
<ul width="100%" class="bord">
 +
</ul>
 +
</nav>
 +
<table style="border-collapse: separate; border-spacing: 0px; width:100%">
 +
<tr><td height="25px"></td></tr>
 +
<tr><td align=center valign="bottom">
 +
<H1>Welcome on the iGEM Grenoble-Alpes 2018 wiki !</H1></td></tr>
 +
<tr><td align=center style="background:#FFFFFF; margin=0px; padding=0px"><IMG SRC="https://static.igem.org/mediawiki/2018/0/0d/T--Grenoble-Alpes--logo.png" ></td></tr>
 +
 +
</table>
 +
--->
 +
 +
 +
 +
<!---
 +
<div class="column full_size" >
 +
<h1> Welcome on the iGEM Grenoble-Alpes 2018 wiki ! </h1>
 +
 +
 +
<p>Your team has been approved and you are ready to start the iGEM season! </p>
 +
 +
 +
<img src="http://placehold.it/1080x320/c4baba/e4dede">
 +
 +
 +
</div>
 +
 +
 +
<div class="column full_size" >
 +
 +
<h3>Before you start</h3>
 +
<p> Please read the following pages:</p>
 +
<ul>
 +
<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
 +
<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 +
<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 +
</ul>
 +
</div>
 +
 +
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
 +
 +
 +
 +
<div class="column full_size" >
 +
<h3> Styling your wiki </h3>
 +
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 +
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 +
 +
</div>
 +
 +
 +
 +
 +
<div class="clear extra_space"></div>
 +
 +
 +
 +
<div class="column third_size" >
 +
 +
<h3> Uploading pictures and files </h3>
 +
<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
 +
 +
 +
<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 +
 +
<div class="button_link">
 +
<a href="https://2018.igem.org/Special:Upload">
 +
UPLOAD FILES
 +
</a>
 +
</div>
 +
 +
</div>
 +
 +
<div class="column third_size" >
 +
<h3> Wiki template information </h3>
 +
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 +
 +
</div>
 +
 +
 +
 +
<div class="column third_size" >
 +
<div class="highlight decoration_B_full">
 +
<h3> Editing your wiki </h3>
 +
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 +
<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
 +
 +
<div class="button_link">
 +
<a href="https://2018.igem.org/wiki/index.php?title=Team:Grenoble-Alpes&action=edit">
 +
EDIT PAGE
 +
</a>
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
 +
 +
 +
 +
<div class="column two_thirds_size" >
 +
<h3>Tips</h3>
 +
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 +
<ul>
 +
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 +
<li>Be clear about what you are doing and how you plan to do this.</li>
 +
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 +
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 +
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 +
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
 +
<li>Have lots of fun! </li>
 +
</ul>
 +
</div>
 +
 +
 +
<div class="column third_size">
 +
<div class="highlight decoration_A_full">
 +
<h3>Inspiration</h3>
 +
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 +
<ul>
 +
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 +
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 +
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 +
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 +
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 +
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
-->
 +
 +
 +
  
 
</html>
 
</html>

Revision as of 13:15, 22 August 2018

Template loop detected: Template:Grenoble-Alpes

Welcome on the iGEM Grenoble-Alpes wiki !

Project description


According to a report from the World Health Organization (WHO), in 2050, each year ten million people will die from pathogenic bacteria that acquired antibiotic resistance if nothing is done.

This is why it is absolutely necessary to find an alternative that can counterbalance this growing antibioresistance. A solution, which is already used in Eastern European countries such as Georgia, is phagotherapy.

This medicine is based on bacteriophages (also nicknamed phages) which are little viruses (from 50 to 200 nm) that can specifically infect a bacterial strain and amplify in it, sometimes leading to the cell death (lytic phages).

This year, the goal of our team is to develop a fully automated system capable of :
  • identifying a pathogenic bacteria
  • detecting resistance markers
  • select the right phages for a possible phagotherapy.

To do this, we will use a DNA probe : a plasmid with a fluorescent gene and a one-stranded fraction which is complementary to the sequence we want to detect. Our automated system will achieve detection from a patient saliva sample. The device will first lyse the bacteria using the phages we are testing and lysis buffer as control. Then, DNA will be extracted using magnetic beads coated with silica. The purified DNA will be digested with the right restriction enzymes to isolate our fraction of interest. The next step will be the hybridization of these fragments with our DNA probes leading to its circularization. Following this, a transformation of the plasmid will be performed into E. Coli competent cells to allow expression of the fluorescence gene and detection.

Our system will be made so the practitioner only has to load samples, run the protocol and come to see the results a few hours later.

For our proof of concept, we will work on two pathogenic bacterial species usually found in nosocomial infection cases due to their ability to develop antibioc resistance : Pseudomonas Aeruginosa and Staphylococcus Aureus. We will make two different probes for each : one for detection of the bacteria, which consists on the recognition of housekeeping genes, and the other for detection of a resistance marker (point mutations on a gene encoding the bacterial wall for example). The two sequences will be detected by fluorescence, using RFP (red fluorescent protein) for housekeeping genes and BFP (blue fluorescent protein) for resistance.

Have a look at our video !


CONTACT


igem.grenoble.alpes@gmail.com


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