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| | <h1 id="welcome-to-our-glorious-landing-page-">Welcome to our glorious Landing Page!</h1> | | <h1 id="welcome-to-our-glorious-landing-page-">Welcome to our glorious Landing Page!</h1> |
| − | <h2 id="test-subheading">Test subheading</h2>
| + | <p>We will have an interactive activity here!</p> |
| − | <p>Heterogeneity of cell populations caused by <span class="tooltip">quorum sensing<span class="shortdef">The ability to detect and to respond to cell population density by gene regulation.</span><span class="longdef" tabindex="1">undefined</span> </span> leads to variability in gene expression that is hard to predict. During biomanufacturing, elevating quorum-induced protein expression will lead to gain of profit. Decreasing this expression can also be beneficial in situations where undesirable <span class="tooltip">biofilms<span class="shortdef">A protective, adhesive matrix of polymers typically produced after quorum activation.</span><span class="longdef" tabindex="1">undefined</span> </span> may form on medical equipment or controlling <span class="tooltip">virulence<span class="shortdef">The likelihood of a microbe to cause disease based on its phenotypic state.</span><span class="longdef" tabindex="1">undefined</span> </span> in bacteria. We will modify the existing bacterial quorum sensing system controlled by the <span class="tooltip">Lsr operon<span class="shortdef">LuxS Regulated (Lsr) operon responsive to AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span> by upregulating the synthesis and excretion of <span class="tooltip">Autoinducer-2<span class="shortdef">A universal signaling molecule used by microorganisms to coordinate group behavior through quorum sensing.</span><span class="longdef" tabindex="1">undefined</span> </span>, a universal quorum molecule. This will increase population-scale AI-2 intake and <span class="tooltip">phosphorylation<span class="shortdef"> The addition of a phosphate group to an organic compound. </span><span class="longdef" tabindex="1">undefined</span> </span> after the initial AI-2 threshold concentrations have been reached to reduce variability in induced gene expression.</p> | + | |
| − | <h2 id="another-test-subheading">Another test subheading</h2>
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| − | <p>A model is used to predict the impacts of manipulating the expression of quorum sensing genes and guide design and experimentation. DNA assembly of a Biobricks containing <span class="tooltip">pLsr<span class="shortdef">The bidirectional promoter of the Lsr Operon.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">T7 RNA Polymerase<span class="shortdef">An RNA polymerase from the T7 bacteriophage is highly selective for the pT7 promoter.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">LsrK<span class="shortdef">AI-2 kinase, which catalyzes the phosphorylation of A1-2 to phospho-AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">LsrACDB<span class="shortdef">Active import protein for AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">LuxS<span class="shortdef">An enzyme closely linked to the production of AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">YdgG<span class="shortdef">Active export protein for AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span>, and superfolding Green Fluorescent Protein (<span class="tooltip">sfGFP<span class="shortdef">superfolding Green Fluorescent Protein</span><span class="longdef" tabindex="1">undefined</span> </span>) enables both the enhancement of natural quorum sensing and the quantification of protein activation among the bacteria in a colony. This device will improve the viability of <span class="tooltip">autoinduction<span class="shortdef">The activation of a phenotype without external stimuli.</span><span class="longdef" tabindex="1">undefined</span> </span> as an induction method in industries such as biomanufacturing by decreasing the variability of a cell phenotypes and increasing expression within a single culture to reduce costs leading to an increase in profits. In addition, by insertion of T7 polymerase into a quorum-sensitive region of the genome of E. coli, we will create a chassis with specific, customizable quorum response for engineering and scientific applications.</p>
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| − | <h1 id="a-second-article">A second article</h1>
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| − | <p>Nullam eu ante vel est convallis dignissim. Fusce suscipit, wisi nec facilisis facilisis, est dui fermentum leo, quis tempor ligula erat quis odio. Nunc porta vulputate tellus. Nunc rutrum turpis sed pede. Sed bibendum. Aliquam posuere. Nunc aliquet, augue nec adipiscing interdum, lacus tellus malesuada massa, quis varius mi purus non odio. Pellentesque condimentum, magna ut suscipit hendrerit, ipsum augue ornare nulla, non luctus diam neque sit amet urna. Curabitur vulputate vestibulum lorem. Fusce sagittis, libero non molestie mollis, magna orci ultrices dolor, at vulputate neque nulla lacinia eros. Sed id ligula quis est convallis tempor. Curabitur lacinia pulvinar nibh. Nam a sapien.</p>
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| − | <h2 id="so-many-subheadings">so many subheadings</h2>
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| − | <p>Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec hendrerit tempor tellus. Donec pretium posuere tellus. Proin quam nisl, tincidunt et, mattis eget, convallis nec, purus. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Nulla posuere. Donec vitae dolor. Nullam tristique diam non turpis. Cras placerat accumsan nulla. Nullam rutrum. Nam vestibulum accumsan nisl.</p>
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| − | <p>Aliquam erat volutpat. Nunc eleifend leo vitae magna. In id erat non orci commodo lobortis. Proin neque massa, cursus ut, gravida ut, lobortis eget, lacus. Sed diam. Praesent fermentum tempor tellus. Nullam tempus. Mauris ac felis vel velit tristique imperdiet. Donec at pede. Etiam vel neque nec dui dignissim bibendum. Vivamus id enim. Phasellus neque orci, porta a, aliquet quis, semper a, massa. Phasellus purus. Pellentesque tristique imperdiet tortor. Nam euismod tellus id erat.</p>
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| − | <h2 id="so-many-subheadings-">so many subheadings!!!</h2>
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| − | <p>Aliquam erat volutpat. Nunc eleifend leo vitae magna. In id erat non orci commodo lobortis. Proin neque massa, cursus ut, gravida ut, lobortis eget, lacus. Sed diam. Praesent fermentum tempor tellus. Nullam tempus. Mauris ac felis vel velit tristique imperdiet. Donec at pede. Etiam vel neque nec dui dignissim bibendum. Vivamus id enim. Phasellus neque orci, porta a, aliquet quis, semper a, massa. Phasellus purus. Pellentesque tristique imperdiet tortor. Nam euismod tellus id erat.</p>
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| − | <p>Nullam eu ante vel est convallis dignissim. Fusce suscipit, wisi nec facilisis facilisis, est dui fermentum leo, quis tempor ligula erat quis odio. Nunc porta vulputate tellus. Nunc rutrum turpis sed pede. Sed bibendum. Aliquam posuere. Nunc aliquet, augue nec adipiscing interdum, lacus tellus malesuada massa, quis varius mi purus non odio. Pellentesque condimentum, magna ut suscipit hendrerit, ipsum augue ornare nulla, non luctus diam neque sit amet urna. Curabitur vulputate vestibulum lorem. Fusce sagittis, libero non molestie mollis, magna orci ultrices dolor, at vulputate neque nulla lacinia eros. Sed id ligula quis est convallis tempor. Curabitur lacinia pulvinar nibh. Nam a sapien.</p>
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