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| Line 334: |
Line 334: |
| | </div> | | </div> |
| | <!-- ############################################################## Tables ######################################### --> | | <!-- ############################################################## Tables ######################################### --> |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0710" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/10</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>shortAK2</td><td>shortAK2</td><td>B.xylophilus cDNA</td></tr><tr><td>2</td><td>Top1</td><td>Top1</td><td>B.xylophilus cDNA</td></tr><tr><td>3</td><td>long AK2</td><td>long AK2</td><td>B.xylophilus cDNA</td></tr><tr><td>4</td><td>eef-1g</td><td>eef-1g</td><td>B.xylophilus cDNA</td></tr><tr><td>5</td><td>AK1</td><td>AK1</td><td>B.xylophilus cDNA</td></tr><tr><td>6</td><td>Asb</td><td>Asb</td><td>B.xylophilus cDNA</td></tr><tr><td>7</td><td>14-3-3zeta</td><td>14-3-3zeta</td><td>B.xylophilus cDNA</td></tr><tr><td>8</td><td>tropomiosin</td><td>tropomiosin</td><td>B.xylophilus cDNA</td></tr><tr><td>9</td><td>14-3-3 protein</td><td>14-3-3 protein</td><td>B.xylophilus cDNA</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_003_0.jpeg -->
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| − | <!-- IGKU_b1_003_1.jpeg -->
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| − | <!-- IGKU_b1_003_2.jpeg -->
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| − | <!-- IGKU_b1_003_3.jpeg -->
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| − | <!-- IGKU_b1_003_4.jpeg -->
| |
| − | <!-- IGKU_b1_003_5.jpeg -->
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| − | <!-- IGKU_b1_003_6.jpeg -->
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| − | <!-- IGKU_b1_003_7.jpeg -->
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| − | <!-- IGKU_b1_003_8.jpeg -->
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| − | <!-- IGKU_b1_003_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">PCR Polymerase : 5*Q5 Polymerase <br>98.0°C : 30sec<br>98.0°C : 10sec<br>58.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 1min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: lane 2~9<br>Gel extraction: lane 2~9</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0711" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/11</h3></span>
| |
| − | <span class="keln_exp"><h4>Miniprep_Nanodrop</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Sample name</th><th>260/280</th><th>260/230</th><th>ng/ul</th></tr><tr><td>1</td><td>2</td><td>no data</td><td>no data</td><td>237.8</td></tr><tr><td>2</td><td>3</td><td>no data</td><td>no data</td><td>180.5</td></tr><tr><td>3</td><td>4</td><td>no data</td><td>no data</td><td>185.6</td></tr><tr><td>4</td><td>5</td><td>no data</td><td>no data</td><td>167.2</td></tr><tr><td>5</td><td>6</td><td>no data</td><td>no data</td><td>328.2</td></tr><tr><td>6</td><td>7</td><td>no data</td><td>no data</td><td>133.7</td></tr><tr><td>7</td><td>8</td><td>no data</td><td>no data</td><td>168.4</td></tr><tr><td>8</td><td>9</td><td>no data</td><td>no data</td><td>242.5</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_004_0.jpeg -->
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| − | <!-- IGKU_b1_004_1.jpeg -->
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| − | <!-- IGKU_b1_004_2.jpeg -->
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| − | <!-- IGKU_b1_004_3.jpeg -->
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| − | <!-- IGKU_b1_004_4.jpeg -->
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| − | <!-- IGKU_b1_004_5.jpeg -->
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| − | <!-- IGKU_b1_004_6.jpeg -->
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| − | <!-- IGKU_b1_004_7.jpeg -->
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| − | <!-- IGKU_b1_004_8.jpeg -->
| |
| − | <!-- IGKU_b1_004_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">The way of extraction : Gel extraction</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0711" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/11</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>1</td><td>Template2</td><td>1.0</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>1</td><td>Template3</td><td>1.0</td></tr><tr><td>3</td><td>YEplac195/sma1</td><td>1</td><td>Template4</td><td>1.0</td></tr><tr><td>4</td><td>YEplac195/sma1</td><td>1</td><td>Template5</td><td>1.0</td></tr><tr><td>5</td><td>YEplac195/sma1</td><td>1</td><td>Template6</td><td>1.0</td></tr><tr><td>6</td><td>YEplac195/sma1</td><td>1</td><td>Template7</td><td>1.0</td></tr><tr><td>7</td><td>YEplac195/sma1</td><td>1</td><td>Template8</td><td>1.0</td></tr><tr><td>8</td><td>YEplac195/sma1</td><td>1</td><td>Template9</td><td>1.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_005_0.jpeg -->
| |
| − | <!-- IGKU_b1_005_1.jpeg -->
| |
| − | <!-- IGKU_b1_005_2.jpeg -->
| |
| − | <!-- IGKU_b1_005_3.jpeg -->
| |
| − | <!-- IGKU_b1_005_4.jpeg -->
| |
| − | <!-- IGKU_b1_005_5.jpeg -->
| |
| − | <!-- IGKU_b1_005_6.jpeg -->
| |
| − | <!-- IGKU_b1_005_7.jpeg -->
| |
| − | <!-- IGKU_b1_005_8.jpeg -->
| |
| − | <!-- IGKU_b1_005_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0711" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/11</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>7/11 ligation product</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_005_0.jpeg -->
| |
| − | <!-- IGKU_b1_005_1.jpeg -->
| |
| − | <!-- IGKU_b1_005_2.jpeg -->
| |
| − | <!-- IGKU_b1_005_3.jpeg -->
| |
| − | <!-- IGKU_b1_005_4.jpeg -->
| |
| − | <!-- IGKU_b1_005_5.jpeg -->
| |
| − | <!-- IGKU_b1_005_6.jpeg -->
| |
| − | <!-- IGKU_b1_005_7.jpeg -->
| |
| − | <!-- IGKU_b1_005_8.jpeg -->
| |
| − | <!-- IGKU_b1_005_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0712" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/12</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>pRS315</td><td>1</td><td>Template</td><td>1.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_006_0.jpeg -->
| |
| − | <!-- IGKU_b1_006_1.jpeg -->
| |
| − | <!-- IGKU_b1_006_2.jpeg -->
| |
| − | <!-- IGKU_b1_006_3.jpeg -->
| |
| − | <!-- IGKU_b1_006_4.jpeg -->
| |
| − | <!-- IGKU_b1_006_5.jpeg -->
| |
| − | <!-- IGKU_b1_006_6.jpeg -->
| |
| − | <!-- IGKU_b1_006_7.jpeg -->
| |
| − | <!-- IGKU_b1_006_8.jpeg -->
| |
| − | <!-- IGKU_b1_006_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0712" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/12</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>7/12 ligation product 2</td></tr><tr><td>2</td><td>DH5a</td><td>7/12 ligation product 3</td></tr><tr><td>3</td><td>DH5a</td><td>7/12 ligation product 4</td></tr><tr><td>4</td><td>DH5a</td><td>7/12 ligation product 5</td></tr><tr><td>5</td><td>DH5a</td><td>7/12 ligation product 6</td></tr><tr><td>6</td><td>DH5a</td><td>7/12 ligation product 7</td></tr><tr><td>7</td><td>DH5a</td><td>7/12 ligation product 8</td></tr><tr><td>8</td><td>DH5a</td><td>7/12 ligation product 9</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_006_0.jpeg -->
| |
| − | <!-- IGKU_b1_006_1.jpeg -->
| |
| − | <!-- IGKU_b1_006_2.jpeg -->
| |
| − | <!-- IGKU_b1_006_3.jpeg -->
| |
| − | <!-- IGKU_b1_006_4.jpeg -->
| |
| − | <!-- IGKU_b1_006_5.jpeg -->
| |
| − | <!-- IGKU_b1_006_6.jpeg -->
| |
| − | <!-- IGKU_b1_006_7.jpeg -->
| |
| − | <!-- IGKU_b1_006_8.jpeg -->
| |
| − | <!-- IGKU_b1_006_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0713" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/13</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>T7Top1</td><td>T7Top1</td><td>7/10 gel extraction product2</td></tr><tr><td>2</td><td>T7long AK2</td><td>T7long AK2</td><td>7/10 gel extraction product3</td></tr><tr><td>3</td><td>T7eef-1g</td><td>T7eef-1g</td><td>7/10 gel extraction product4</td></tr><tr><td>4</td><td>T7AK1</td><td>T7AK1</td><td>7/10 gel extraction product5</td></tr><tr><td>5</td><td>T7Asb</td><td>T7Asb</td><td>7/10 gel extraction product6</td></tr><tr><td>6</td><td>T714-3-3zeta</td><td>T714-3-3zeta</td><td>7/10 gel extraction product7</td></tr><tr><td>7</td><td>T7tropomiosin</td><td>T7tropomiosin</td><td>7/10 gel extraction product8</td></tr><tr><td>8</td><td>T714-3-3 protein</td><td>T714-3-3 protein</td><td>7/10 gel extraction product9</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_007_0.jpeg -->
| |
| − | <!-- IGKU_b1_007_1.jpeg -->
| |
| − | <!-- IGKU_b1_007_2.jpeg -->
| |
| − | <!-- IGKU_b1_007_3.jpeg -->
| |
| − | <!-- IGKU_b1_007_4.jpeg -->
| |
| − | <!-- IGKU_b1_007_5.jpeg -->
| |
| − | <!-- IGKU_b1_007_6.jpeg -->
| |
| − | <!-- IGKU_b1_007_7.jpeg -->
| |
| − | <!-- IGKU_b1_007_8.jpeg -->
| |
| − | <!-- IGKU_b1_007_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">PCR Polymerase : ExTaq <br>98.0°C : 1min<br>98.0°C : 10sec<br>58.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 1min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0714" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/14</h3></span>
| |
| − | <span class="keln_exp"><h4>Miniprep_Nanodrop</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Sample name</th><th>260/280</th><th>260/230</th><th>ng/ul</th></tr><tr><td>1</td><td>1</td><td>1.84</td><td>1.96</td><td>98.4</td></tr><tr><td>2</td><td>2</td><td>1.79</td><td>1.85</td><td>76.2</td></tr><tr><td>3</td><td>3</td><td>1.85</td><td>2.1</td><td>102.1</td></tr><tr><td>4</td><td>4</td><td>1.62</td><td>0.49</td><td>9.5</td></tr><tr><td>5</td><td>5</td><td>1.91</td><td>1.91</td><td>83.6</td></tr><tr><td>6</td><td>6</td><td>1.82</td><td>1.73</td><td>48.0</td></tr><tr><td>7</td><td>7</td><td>1.85</td><td>1.85</td><td>83.0</td></tr><tr><td>8</td><td>8</td><td>1.86</td><td>2.84</td><td>81.5</td></tr><tr><td>9</td><td>9</td><td>1.57</td><td>0.33</td><td>22.6</td></tr><tr><td>10</td><td>10</td><td>1.54</td><td>0.55</td><td>17.8</td></tr><tr><td>11</td><td>11</td><td>1.83</td><td>2.02</td><td>88.0</td></tr><tr><td>12</td><td>12.0</td><td>1.85</td><td>2.94</td><td>82.0</td></tr><tr><td>13</td><td>13.0</td><td>1.83</td><td>1.83</td><td>90.6</td></tr><tr><td>14</td><td>14.0</td><td>1.87</td><td>1.65</td><td>46.2</td></tr><tr><td>15</td><td>15.0</td><td>1.89</td><td>2.0</td><td>75.8</td></tr><tr><td>16</td><td>16.0</td><td>1.67</td><td>1.98</td><td>114.0</td></tr><tr><td>17</td><td>17.0</td><td>1.88</td><td>1.46</td><td>88.5</td></tr><tr><td>18</td><td>18.0</td><td>1.85</td><td>1.91</td><td>99.4</td></tr><tr><td>19</td><td>19.0</td><td>1.79</td><td>1.79</td><td>53.8</td></tr><tr><td>20</td><td>20.0</td><td>1.37</td><td>0.58</td><td>17.0</td></tr><tr><td>21</td><td>21.0</td><td>1.63</td><td>0.57</td><td>0.57</td></tr><tr><td>22</td><td>22.0</td><td>1.83</td><td>1.84</td><td>53.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_008_0.jpeg -->
| |
| − | <!-- IGKU_b1_008_1.jpeg -->
| |
| − | <!-- IGKU_b1_008_2.jpeg -->
| |
| − | <!-- IGKU_b1_008_3.jpeg -->
| |
| − | <!-- IGKU_b1_008_4.jpeg -->
| |
| − | <!-- IGKU_b1_008_5.jpeg -->
| |
| − | <!-- IGKU_b1_008_6.jpeg -->
| |
| − | <!-- IGKU_b1_008_7.jpeg -->
| |
| − | <!-- IGKU_b1_008_8.jpeg -->
| |
| − | <!-- IGKU_b1_008_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">The way of extraction : promega</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0715" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/15</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>1</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>2</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>3</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>4</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>5</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>6</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>7</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>8</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>9</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>10</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>11</td><td>11</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>12</td><td>12</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>13</td><td>13</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>14</td><td>14</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>15</td><td>15</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>16</td><td>16</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>17</td><td>17</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>18</td><td>18</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>19</td><td>19</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>20</td><td>20</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>21</td><td>21</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>22</td><td>22</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>Sac1</td><td>0.05</td><td>10xM</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_011_0.jpeg -->
| |
| − | <!-- IGKU_b1_011_1.jpeg -->
| |
| − | <!-- IGKU_b1_011_2.jpeg -->
| |
| − | <!-- IGKU_b1_011_3.jpeg -->
| |
| − | <!-- IGKU_b1_011_4.jpeg -->
| |
| − | <!-- IGKU_b1_011_5.jpeg -->
| |
| − | <!-- IGKU_b1_011_6.jpeg -->
| |
| − | <!-- IGKU_b1_011_7.jpeg -->
| |
| − | <!-- IGKU_b1_011_8.jpeg -->
| |
| − | <!-- IGKU_b1_011_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0716" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/16</h3></span>
| |
| − | <span class="keln_exp"><h4>Miniprep_Nanodrop</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Sample name</th><th>260/280</th><th>260/230</th><th>ng/ul</th></tr><tr><td>1</td><td>T7 3</td><td>1.86</td><td>2.28</td><td>255.7</td></tr><tr><td>2</td><td>T7 4</td><td>1.83</td><td>2.13</td><td>121.9</td></tr><tr><td>3</td><td>T7 5</td><td>1.83</td><td>2.29</td><td>224.2</td></tr><tr><td>4</td><td>T7 6</td><td>1.85</td><td>2.18</td><td>146.1</td></tr><tr><td>5</td><td>T7 7</td><td>1.85</td><td>2.25</td><td>96.5</td></tr><tr><td>6</td><td>T7 8</td><td>1.85</td><td>2.27</td><td>165.4</td></tr><tr><td>7</td><td>T7 9</td><td>No data</td><td>No data</td><td>No data</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_012_0.jpeg -->
| |
| − | <!-- IGKU_b1_012_1.jpeg -->
| |
| − | <!-- IGKU_b1_012_2.jpeg -->
| |
| − | <!-- IGKU_b1_012_3.jpeg -->
| |
| − | <!-- IGKU_b1_012_4.jpeg -->
| |
| − | <!-- IGKU_b1_012_5.jpeg -->
| |
| − | <!-- IGKU_b1_012_6.jpeg -->
| |
| − | <!-- IGKU_b1_012_7.jpeg -->
| |
| − | <!-- IGKU_b1_012_8.jpeg -->
| |
| − | <!-- IGKU_b1_012_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">The way of extraction : promega</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0716" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>07/16</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>short AK2</td><td>short AK2</td><td>B.xylophilus cDNA</td></tr><tr><td>2</td><td>Top1</td><td>Top1</td><td>B.xylophilus cDNA</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_014_0.jpeg -->
| |
| − | <!-- IGKU_b1_014_1.jpeg -->
| |
| − | <!-- IGKU_b1_014_2.jpeg -->
| |
| − | <!-- IGKU_b1_014_3.jpeg -->
| |
| − | <!-- IGKU_b1_014_4.jpeg -->
| |
| − | <!-- IGKU_b1_014_5.jpeg -->
| |
| − | <!-- IGKU_b1_014_6.jpeg -->
| |
| − | <!-- IGKU_b1_014_7.jpeg -->
| |
| − | <!-- IGKU_b1_014_8.jpeg -->
| |
| − | <!-- IGKU_b1_014_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">PCR Polymerase : Extaq <br>94.0°C : 1min<br>94.0°C : 30sec<br>60.0°C : 1min<br>72.0°C : 2min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0806" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/06</h3></span>
| |
| − | <span class="keln_exp"><h4>Nebuilder</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>Sample</th><th>Vectorname</th><th>Vector amount (ul)</th><th> Insert1 name</th><th> Insert1 amount(ul)</th><th>Insert2 name </th><th>Insert2 amount (ul)</th><th>Mastermix amount (ul)</th><th>Total amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>2.5</td><td>Gal-AK</td><td>0.8</td><td>Ter-AK</td><td>1.0</td><td>10.0</td><td>20.0</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>2.5</td><td>GPD-AK</td><td>1.2</td><td>Ter-AK</td><td>1.0</td><td>10.0</td><td>20.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_016_0.jpeg -->
| |
| − | <!-- IGKU_b1_016_1.jpeg -->
| |
| − | <!-- IGKU_b1_016_2.jpeg -->
| |
| − | <!-- IGKU_b1_016_3.jpeg -->
| |
| − | <!-- IGKU_b1_016_4.jpeg -->
| |
| − | <!-- IGKU_b1_016_5.jpeg -->
| |
| − | <!-- IGKU_b1_016_6.jpeg -->
| |
| − | <!-- IGKU_b1_016_7.jpeg -->
| |
| − | <!-- IGKU_b1_016_8.jpeg -->
| |
| − | <!-- IGKU_b1_016_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0806" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/06</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>Insert</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_017_0.jpeg -->
| |
| − | <!-- IGKU_b1_017_1.jpeg -->
| |
| − | <!-- IGKU_b1_017_2.jpeg -->
| |
| − | <!-- IGKU_b1_017_3.jpeg -->
| |
| − | <!-- IGKU_b1_017_4.jpeg -->
| |
| − | <!-- IGKU_b1_017_5.jpeg -->
| |
| − | <!-- IGKU_b1_017_6.jpeg -->
| |
| − | <!-- IGKU_b1_017_7.jpeg -->
| |
| − | <!-- IGKU_b1_017_8.jpeg -->
| |
| − | <!-- IGKU_b1_017_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0807" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/07</h3></span>
| |
| − | <span class="keln_exp"><h4>Miniprep_Nanodrop</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Sample name</th><th>260/280</th><th>260/230</th><th>ng/ul</th></tr><tr><td>1</td><td>1</td><td>1.97</td><td>2.03</td><td>36.0</td></tr><tr><td>2</td><td>2</td><td>2.03</td><td>1.89</td><td>28.7</td></tr><tr><td>3</td><td>3</td><td>1.92</td><td>1.98</td><td>34.3</td></tr><tr><td>4</td><td>4</td><td>1.96</td><td>1.88</td><td>35.5</td></tr><tr><td>5</td><td>5</td><td>2.03</td><td>1.87</td><td>16.2</td></tr><tr><td>6</td><td>6</td><td>2.01</td><td>2.11</td><td>50.0</td></tr><tr><td>7</td><td>7</td><td>1.95</td><td>1.95</td><td>30.3</td></tr><tr><td>8</td><td>8</td><td>1.95</td><td>2.06</td><td>38.2</td></tr><tr><td>9</td><td>9</td><td>2.12</td><td>1.94</td><td>17.0</td></tr><tr><td>10</td><td>10</td><td>no data</td><td>no data</td><td>no data</td></tr><tr><td>11</td><td>11</td><td>1.99</td><td>1.92</td><td>27.9</td></tr><tr><td>12</td><td>12</td><td>1.96</td><td>1.79</td><td>19.1</td></tr><tr><td>13</td><td>13</td><td>1.95</td><td>1.96</td><td>29.4</td></tr><tr><td>14</td><td>14</td><td>1.98</td><td>1.64</td><td>22.7</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_018_0.jpeg -->
| |
| − | <!-- IGKU_b1_018_1.jpeg -->
| |
| − | <!-- IGKU_b1_018_2.jpeg -->
| |
| − | <!-- IGKU_b1_018_3.jpeg -->
| |
| − | <!-- IGKU_b1_018_4.jpeg -->
| |
| − | <!-- IGKU_b1_018_5.jpeg -->
| |
| − | <!-- IGKU_b1_018_6.jpeg -->
| |
| − | <!-- IGKU_b1_018_7.jpeg -->
| |
| − | <!-- IGKU_b1_018_8.jpeg -->
| |
| − | <!-- IGKU_b1_018_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">The way of extraction : fastgene plasmid mini kit</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0808" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/08</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>8/6 Nebuilder product</td></tr><tr><td>2</td><td>DH5a</td><td>8/6 Nebuilder product</td></tr><tr><td>3</td><td>DH5a</td><td>8/6 Nebuilder product</td></tr><tr><td>4</td><td>DH5a</td><td>8/6 Nebuilder product</td></tr><tr><td>5</td><td>DH5a</td><td>8/6 Nebuilder product</td></tr><tr><td>6</td><td>DH5a</td><td>8/6 Nebuilder product</td></tr><tr><td>7</td><td>DH5a</td><td>8/6 Nebuilder product</td></tr><tr><td>8</td><td>DH5a</td><td>8/6 Nebuilder product</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_022_0.jpeg -->
| |
| − | <!-- IGKU_b1_022_1.jpeg -->
| |
| − | <!-- IGKU_b1_022_2.jpeg -->
| |
| − | <!-- IGKU_b1_022_3.jpeg -->
| |
| − | <!-- IGKU_b1_022_4.jpeg -->
| |
| − | <!-- IGKU_b1_022_5.jpeg -->
| |
| − | <!-- IGKU_b1_022_6.jpeg -->
| |
| − | <!-- IGKU_b1_022_7.jpeg -->
| |
| − | <!-- IGKU_b1_022_8.jpeg -->
| |
| − | <!-- IGKU_b1_022_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0808" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/08</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>miniprep 1</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>miniprep 2</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>miniprep 3</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>miniprep 4</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>miniprep 5</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>miniprep 7</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_023_0.jpeg -->
| |
| − | <!-- IGKU_b1_023_1.jpeg -->
| |
| − | <!-- IGKU_b1_023_2.jpeg -->
| |
| − | <!-- IGKU_b1_023_3.jpeg -->
| |
| − | <!-- IGKU_b1_023_4.jpeg -->
| |
| − | <!-- IGKU_b1_023_5.jpeg -->
| |
| − | <!-- IGKU_b1_023_6.jpeg -->
| |
| − | <!-- IGKU_b1_023_7.jpeg -->
| |
| − | <!-- IGKU_b1_023_8.jpeg -->
| |
| − | <!-- IGKU_b1_023_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0809" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/09</h3></span>
| |
| − | <span class="keln_exp"><h4>Miniprep_Nanodrop</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Sample name</th><th>260/280</th><th>260/230</th><th>ng/ul</th></tr><tr><td>1</td><td>8</td><td>1.69</td><td>1.32</td><td>12.1</td></tr><tr><td>2</td><td>9</td><td>1.97</td><td>1.45</td><td>17.0</td></tr><tr><td>3</td><td>11</td><td>1.83</td><td>0.99</td><td>12.5</td></tr><tr><td>4</td><td>12</td><td>1.92</td><td>1.05</td><td>12.3</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_026_0.jpeg -->
| |
| − | <!-- IGKU_b1_026_1.jpeg -->
| |
| − | <!-- IGKU_b1_026_2.jpeg -->
| |
| − | <!-- IGKU_b1_026_3.jpeg -->
| |
| − | <!-- IGKU_b1_026_4.jpeg -->
| |
| − | <!-- IGKU_b1_026_5.jpeg -->
| |
| − | <!-- IGKU_b1_026_6.jpeg -->
| |
| − | <!-- IGKU_b1_026_7.jpeg -->
| |
| − | <!-- IGKU_b1_026_8.jpeg -->
| |
| − | <!-- IGKU_b1_026_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">The way of extraction : promega PureYield Plasmid Midiprepkit</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0809" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/09</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>8/8 miniprep8</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>8/8 miniprep9</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>8/8 miniprep11</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>8/8 miniprep12</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <img src="https://static.igem.org/mediawiki/2017/c/c0/IGKU_b1_027_0.jpeg">
| |
| − | <!-- IGKU_b1_027_1.jpeg -->
| |
| − | <!-- IGKU_b1_027_2.jpeg -->
| |
| − | <!-- IGKU_b1_027_3.jpeg -->
| |
| − | <!-- IGKU_b1_027_4.jpeg -->
| |
| − | <!-- IGKU_b1_027_5.jpeg -->
| |
| − | <!-- IGKU_b1_027_6.jpeg -->
| |
| − | <!-- IGKU_b1_027_7.jpeg -->
| |
| − | <!-- IGKU_b1_027_8.jpeg -->
| |
| − | <!-- IGKU_b1_027_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0809" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/09</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Fukuda</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>AK2</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>2</td><td>AK2</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>3</td><td>eef-1g</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>4</td><td>eef-1g</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>5</td><td>AK1</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>6</td><td>AK1</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>7</td><td>Asb</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>8</td><td>Asb</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>9</td><td>14-3-3-zeta</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>10</td><td>14-3-3-zeta</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>11</td><td>tropomiosin</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>12</td><td>tropomiosin</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>13</td><td>14-3-3 protein</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr><tr><td>14</td><td>14-3-3 protein</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>6.0</td><td>36.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_028_0.jpeg -->
| |
| − | <!-- IGKU_b2_028_1.jpeg -->
| |
| − | <!-- IGKU_b2_028_2.jpeg -->
| |
| − | <!-- IGKU_b2_028_3.jpeg -->
| |
| − | <!-- IGKU_b2_028_4.jpeg -->
| |
| − | <!-- IGKU_b2_028_5.jpeg -->
| |
| − | <!-- IGKU_b2_028_6.jpeg -->
| |
| − | <!-- IGKU_b2_028_7.jpeg -->
| |
| − | <!-- IGKU_b2_028_8.jpeg -->
| |
| − | <!-- IGKU_b2_028_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0810" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/10</h3></span>
| |
| − | <span class="keln_exp"><h4>Nebuilder</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>Sample</th><th>Vectorname</th><th>Vector amount (ul)</th><th> Insert1 name</th><th> Insert1 amount(ul)</th><th>Insert2 name </th><th>Insert2 amount (ul)</th><th>Mastermix amount (ul)</th><th>Total amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>2.5</td><td>Gal-AK</td><td>0.8</td><td>Ter-AK</td><td>1.0</td><td>10.0</td><td>20.0</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>2.5</td><td>GPD-AK</td><td>1.2</td><td>Ter-AK</td><td>1.0</td><td>10.0</td><td>20.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_028_0.jpeg -->
| |
| − | <!-- IGKU_b1_028_1.jpeg -->
| |
| − | <!-- IGKU_b1_028_2.jpeg -->
| |
| − | <!-- IGKU_b1_028_3.jpeg -->
| |
| − | <!-- IGKU_b1_028_4.jpeg -->
| |
| − | <!-- IGKU_b1_028_5.jpeg -->
| |
| − | <!-- IGKU_b1_028_6.jpeg -->
| |
| − | <!-- IGKU_b1_028_7.jpeg -->
| |
| − | <!-- IGKU_b1_028_8.jpeg -->
| |
| − | <!-- IGKU_b1_028_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0810" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/10</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>insert</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_028_0.jpeg -->
| |
| − | <!-- IGKU_b1_028_1.jpeg -->
| |
| − | <!-- IGKU_b1_028_2.jpeg -->
| |
| − | <!-- IGKU_b1_028_3.jpeg -->
| |
| − | <!-- IGKU_b1_028_4.jpeg -->
| |
| − | <!-- IGKU_b1_028_5.jpeg -->
| |
| − | <!-- IGKU_b1_028_6.jpeg -->
| |
| − | <!-- IGKU_b1_028_7.jpeg -->
| |
| − | <!-- IGKU_b1_028_8.jpeg -->
| |
| − | <!-- IGKU_b1_028_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0810" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/10</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>T7AK2F</td><td>T7AK2R</td><td>B.xylophils cDNA1</td></tr><tr><td>2</td><td>T7AK2F</td><td>T7AK2R</td><td>B.xylophils cDNA2</td></tr><tr><td>3</td><td>T7Egf-1gF</td><td>T7Egf-1gR</td><td>B.xylophils cDNA3</td></tr><tr><td>4</td><td>T7Egf-1gF</td><td>T7Egf-1gR</td><td>B.xylophils cDNA4</td></tr><tr><td>5</td><td>T7AK1F</td><td>T7Ak1R</td><td>B.xylophils cDNA5</td></tr><tr><td>6</td><td>T7AK1F</td><td>T7Ak1R</td><td>B.xylophils cDNA6</td></tr><tr><td>7</td><td>T714-3-3zetaF</td><td>T714-3-3 zetaR</td><td>B.xylophils cDNA7</td></tr><tr><td>8</td><td>T714-3-3zetaF</td><td>T714-3-3 zetaR</td><td>B.xylophils cDNA8</td></tr><tr><td>9</td><td>T7tropomiosin F</td><td>T7tropomiosin R</td><td>B.xylophils cDNA9</td></tr><tr><td>10</td><td>T7tropomiosin F</td><td>T7tropomiosin R</td><td>B.xylophils cDNA10</td></tr><tr><td>11</td><td>T714-3-3 F</td><td>T714-3-3 R</td><td>B.xylophils cDNA11</td></tr><tr><td>12</td><td>T714-3-3 F</td><td>T714-3-3 R</td><td>B.xylophils cDNA12</td></tr><tr><td>13</td><td>T7AK2F</td><td>T7AK2R</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : gotaq <br>94.0°C : 2min<br>94.0°C : 30sec<br>62.0°C : 30sec<br>68.0°C : 1min20sec<br>68.0°C : 7min<br>4.0°C : Infinate<br>X30 cycle</p><img src="https://static.igem.org/mediawiki/2017/2/21/IGKU_b2_029_0.jpeg">
| |
| − | <!-- IGKU_b2_029_1.jpeg -->
| |
| − | <!-- IGKU_b2_029_2.jpeg -->
| |
| − | <!-- IGKU_b2_029_3.jpeg -->
| |
| − | <!-- IGKU_b2_029_4.jpeg -->
| |
| − | <!-- IGKU_b2_029_5.jpeg -->
| |
| − | <!-- IGKU_b2_029_6.jpeg -->
| |
| − | <!-- IGKU_b2_029_7.jpeg -->
| |
| − | <!-- IGKU_b2_029_8.jpeg -->
| |
| − | <!-- IGKU_b2_029_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0811" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/11</h3></span>
| |
| − | <span class="keln_exp"><h4>colony_PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>1</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>3</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>5</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>7</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>9</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>11</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>13</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>15</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>17</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>19</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>21</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>23</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>25</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>27</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>29</td></tr><tr><td>16</td><td>M13F</td><td>M13R</td><td>31</td></tr><tr><td>17</td><td>M13F</td><td>M13R</td><td>33</td></tr><tr><td>18</td><td>M13F</td><td>M13R</td><td>35</td></tr><tr><td>19</td><td>M13F</td><td>M13R</td><td>37</td></tr><tr><td>20</td><td>M13F</td><td>M13R</td><td>39</td></tr><tr><td>21</td><td>M13F</td><td>M13R</td><td>41</td></tr><tr><td>22</td><td>M13F</td><td>M13R</td><td>43</td></tr><tr><td>23</td><td>M13F</td><td>M13R</td><td>45</td></tr><tr><td>24</td><td>M13F</td><td>M13R</td><td>47</td></tr><tr><td>25</td><td>M13F</td><td>M13R</td><td>49</td></tr>
| |
| − | </table>
| |
| − | <img src="http://https://2017.igem.org/File:IGKU_b1_030_0.jpeg">
| |
| − | <!-- IGKU_b1_029_1.jpeg -->
| |
| − | <!-- IGKU_b1_029_2.jpeg -->
| |
| − | <!-- IGKU_b1_029_3.jpeg -->
| |
| − | <!-- IGKU_b1_029_4.jpeg -->
| |
| − | <!-- IGKU_b1_029_5.jpeg -->
| |
| − | <!-- IGKU_b1_029_6.jpeg -->
| |
| − | <!-- IGKU_b1_029_7.jpeg -->
| |
| − | <!-- IGKU_b1_029_8.jpeg -->
| |
| − | <!-- IGKU_b1_029_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">PCR Polymerase : QuickTaq <br>94.0°C : 2min<br>94.0°C : 30sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 1min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0811" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/11</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>1-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>2</td><td>1-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>3</td><td>1-3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>4</td><td>1-4</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>5</td><td>1-5</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>6</td><td>2-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>7</td><td>1-6</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>8</td><td>2-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>9</td><td>2-3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>10</td><td>2-4</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>11</td><td>2-5</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>12</td><td>2-6</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>13</td><td>3-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>14</td><td>3-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>15</td><td>3-3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr><tr><td>16</td><td>3-4</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>3.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/a/af/IGKU_b2_030_0.jpeg">
| |
| − | <!-- IGKU_b2_030_1.jpeg -->
| |
| − | <!-- IGKU_b2_030_2.jpeg -->
| |
| − | <!-- IGKU_b2_030_3.jpeg -->
| |
| − | <!-- IGKU_b2_030_4.jpeg -->
| |
| − | <!-- IGKU_b2_030_5.jpeg -->
| |
| − | <!-- IGKU_b2_030_6.jpeg -->
| |
| − | <!-- IGKU_b2_030_7.jpeg -->
| |
| − | <!-- IGKU_b2_030_8.jpeg -->
| |
| − | <!-- IGKU_b2_030_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0811" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/11</h3></span>
| |
| − | <span class="keln_exp"><h4>Miniprep_Nanodrop</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Sample name</th><th>260/280</th><th>260/230</th><th>ng/ul</th></tr><tr><td>1</td><td>1</td><td>1.88</td><td>1.6</td><td>116.8</td></tr><tr><td>2</td><td>8</td><td>1.9</td><td>1.67</td><td>170.0</td></tr><tr><td>3</td><td>9</td><td>1.91</td><td>1.71</td><td>167.6</td></tr><tr><td>4</td><td>12</td><td>1.88</td><td>1.6</td><td>135.6</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_031_0.jpeg -->
| |
| − | <!-- IGKU_b1_031_1.jpeg -->
| |
| − | <!-- IGKU_b1_031_2.jpeg -->
| |
| − | <!-- IGKU_b1_031_3.jpeg -->
| |
| − | <!-- IGKU_b1_031_4.jpeg -->
| |
| − | <!-- IGKU_b1_031_5.jpeg -->
| |
| − | <!-- IGKU_b1_031_6.jpeg -->
| |
| − | <!-- IGKU_b1_031_7.jpeg -->
| |
| − | <!-- IGKU_b1_031_8.jpeg -->
| |
| − | <!-- IGKU_b1_031_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">The way of extraction : promega PureYield Plasmid Midiprepkit</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0811" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/11</h3></span>
| |
| − | <span class="keln_exp"><h4>colony_PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>2</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>4</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>6</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>8</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>10</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>12</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>14</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>16</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>18</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>20</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>22</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>24</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>26</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>28</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>30</td></tr><tr><td>16</td><td>M13F</td><td>M13R</td><td>32</td></tr><tr><td>17</td><td>M13F</td><td>M13R</td><td>34</td></tr><tr><td>18</td><td>M13F</td><td>M13R</td><td>36</td></tr><tr><td>19</td><td>M13F</td><td>M13R</td><td>38</td></tr><tr><td>20</td><td>M13F</td><td>M13R</td><td>40</td></tr><tr><td>21</td><td>M13F</td><td>M13R</td><td>42</td></tr><tr><td>22</td><td>M13F</td><td>M13R</td><td>44</td></tr><tr><td>23</td><td>M13F</td><td>M13R</td><td>46</td></tr><tr><td>24</td><td>M13F</td><td>M13R</td><td>48</td></tr><tr><td>25</td><td>M13F</td><td>M13R</td><td>50</td></tr><tr><td>26</td><td>M13F</td><td>M13R</td><td>53</td></tr><tr><td>27</td><td>M13F</td><td>M13R</td><td>54</td></tr><tr><td>28</td><td>M13F</td><td>M13R</td><td>55</td></tr><tr><td>29</td><td>M13F</td><td>M13R</td><td>56</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_032_0.jpeg -->
| |
| − | <!-- IGKU_b1_032_1.jpeg -->
| |
| − | <!-- IGKU_b1_032_2.jpeg -->
| |
| − | <!-- IGKU_b1_032_3.jpeg -->
| |
| − | <!-- IGKU_b1_032_4.jpeg -->
| |
| − | <!-- IGKU_b1_032_5.jpeg -->
| |
| − | <!-- IGKU_b1_032_6.jpeg -->
| |
| − | <!-- IGKU_b1_032_7.jpeg -->
| |
| − | <!-- IGKU_b1_032_8.jpeg -->
| |
| − | <!-- IGKU_b1_032_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">PCR Polymerase : Quick_taq <br>94.0°C : 2min<br>94.0°C : 30sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 10min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0811" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/11</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>1</td><td>AK2-gal1</td><td>1.0</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>1</td><td>AK2-GPD</td><td>1.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_032_0.jpeg -->
| |
| − | <!-- IGKU_b1_032_1.jpeg -->
| |
| − | <!-- IGKU_b1_032_2.jpeg -->
| |
| − | <!-- IGKU_b1_032_3.jpeg -->
| |
| − | <!-- IGKU_b1_032_4.jpeg -->
| |
| − | <!-- IGKU_b1_032_5.jpeg -->
| |
| − | <!-- IGKU_b1_032_6.jpeg -->
| |
| − | <!-- IGKU_b1_032_7.jpeg -->
| |
| − | <!-- IGKU_b1_032_8.jpeg -->
| |
| − | <!-- IGKU_b1_032_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0811" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/11</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>7A</td><td>5.0</td><td>Pst1</td><td>0.5</td><td>10xH</td><td>6.0</td><td>60.0</td></tr><tr><td>2</td><td>7B</td><td>5.0</td><td>Pst1</td><td>0.5</td><td>10xH</td><td>6.0</td><td>60.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/f/f1/IGKU_b2_032_0.jpeg">
| |
| − | <!-- IGKU_b2_032_1.jpeg -->
| |
| − | <!-- IGKU_b2_032_2.jpeg -->
| |
| − | <!-- IGKU_b2_032_3.jpeg -->
| |
| − | <!-- IGKU_b2_032_4.jpeg -->
| |
| − | <!-- IGKU_b2_032_5.jpeg -->
| |
| − | <!-- IGKU_b2_032_6.jpeg -->
| |
| − | <!-- IGKU_b2_032_7.jpeg -->
| |
| − | <!-- IGKU_b2_032_8.jpeg -->
| |
| − | <!-- IGKU_b2_032_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0811" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/11</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>AK2-gal1 Nebuilder</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>AK2 GPD Nebuilder</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>gfp gal Nebuilder</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>gfp GPD Nebuilder</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>AK1 gal Nebuilder</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>AK1 GPD Nebuilder</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_033_0.jpeg -->
| |
| − | <!-- IGKU_b1_033_1.jpeg -->
| |
| − | <!-- IGKU_b1_033_2.jpeg -->
| |
| − | <!-- IGKU_b1_033_3.jpeg -->
| |
| − | <!-- IGKU_b1_033_4.jpeg -->
| |
| − | <!-- IGKU_b1_033_5.jpeg -->
| |
| − | <!-- IGKU_b1_033_6.jpeg -->
| |
| − | <!-- IGKU_b1_033_7.jpeg -->
| |
| − | <!-- IGKU_b1_033_8.jpeg -->
| |
| − | <!-- IGKU_b1_033_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA 2G Robust HS Readymix <br>94.0°C : 2min<br>94.0°C : 15sec<br>60.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 1min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0811" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/11</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>insert</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_033_0.jpeg -->
| |
| − | <!-- IGKU_b1_033_1.jpeg -->
| |
| − | <!-- IGKU_b1_033_2.jpeg -->
| |
| − | <!-- IGKU_b1_033_3.jpeg -->
| |
| − | <!-- IGKU_b1_033_4.jpeg -->
| |
| − | <!-- IGKU_b1_033_5.jpeg -->
| |
| − | <!-- IGKU_b1_033_6.jpeg -->
| |
| − | <!-- IGKU_b1_033_7.jpeg -->
| |
| − | <!-- IGKU_b1_033_8.jpeg -->
| |
| − | <!-- IGKU_b1_033_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0812" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/12</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>AK1F</td><td>AK1R</td><td>8</td></tr><tr><td>2</td><td>AK1F</td><td>AK1R</td><td>9</td></tr><tr><td>3</td><td>AK1F</td><td>AK1R</td><td>12</td></tr><tr><td>4</td><td>AK1F</td><td>AK1R</td><td>8/6 gal</td></tr><tr><td>5</td><td>AK1F</td><td>AK1R</td><td>8/6 GPD</td></tr><tr><td>6</td><td>AK1F</td><td>AK1R</td><td>gal1 AK1</td></tr><tr><td>7</td><td>AK1F</td><td>AK1R</td><td>gal1 AK1</td></tr><tr><td>8</td><td>AK1F</td><td>AK1R</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : quicktaq <br>94.0°C : 2min<br>94.0°C : 30sec<br>60.0°C : 30sec<br>68.0°C : 1min<br>68.0°C : 7min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/8/85/IGKU_b2_033_0.jpeg">
| |
| − | <!-- IGKU_b2_033_1.jpeg -->
| |
| − | <!-- IGKU_b2_033_2.jpeg -->
| |
| − | <!-- IGKU_b2_033_3.jpeg -->
| |
| − | <!-- IGKU_b2_033_4.jpeg -->
| |
| − | <!-- IGKU_b2_033_5.jpeg -->
| |
| − | <!-- IGKU_b2_033_6.jpeg -->
| |
| − | <!-- IGKU_b2_033_7.jpeg -->
| |
| − | <!-- IGKU_b2_033_8.jpeg -->
| |
| − | <!-- IGKU_b2_033_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0812" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/12</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>1</td><td>gblock 71</td><td>1.0</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>1</td><td>gblock 72</td><td>1.0</td></tr><tr><td>3</td><td>YEplac195/sma1</td><td>1</td><td>gblock 73</td><td>1.0</td></tr><tr><td>4</td><td>YEplac195/sma1</td><td>1</td><td>gblock 74</td><td>1.0</td></tr><tr><td>5</td><td>YEplac195/sma1</td><td>1</td><td>gblock 75</td><td>1.0</td></tr><tr><td>6</td><td>YEplac195/sma1</td><td>1</td><td>gblock 76</td><td>1.0</td></tr><tr><td>7</td><td>YEplac195/sma1</td><td>1</td><td>gblock 77</td><td>1.0</td></tr><tr><td>8</td><td>YEplac195/sma1</td><td>1</td><td>gblock 78</td><td>1.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_036_0.jpeg -->
| |
| − | <!-- IGKU_b1_036_1.jpeg -->
| |
| − | <!-- IGKU_b1_036_2.jpeg -->
| |
| − | <!-- IGKU_b1_036_3.jpeg -->
| |
| − | <!-- IGKU_b1_036_4.jpeg -->
| |
| − | <!-- IGKU_b1_036_5.jpeg -->
| |
| − | <!-- IGKU_b1_036_6.jpeg -->
| |
| − | <!-- IGKU_b1_036_7.jpeg -->
| |
| − | <!-- IGKU_b1_036_8.jpeg -->
| |
| − | <!-- IGKU_b1_036_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0812" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/12</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimito</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>insert</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_036_0.jpeg -->
| |
| − | <!-- IGKU_b1_036_1.jpeg -->
| |
| − | <!-- IGKU_b1_036_2.jpeg -->
| |
| − | <!-- IGKU_b1_036_3.jpeg -->
| |
| − | <!-- IGKU_b1_036_4.jpeg -->
| |
| − | <!-- IGKU_b1_036_5.jpeg -->
| |
| − | <!-- IGKU_b1_036_6.jpeg -->
| |
| − | <!-- IGKU_b1_036_7.jpeg -->
| |
| − | <!-- IGKU_b1_036_8.jpeg -->
| |
| − | <!-- IGKU_b1_036_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0813" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/13</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>AK2F</td><td>AK2F</td><td>8</td></tr><tr><td>2</td><td>AK2F</td><td>AK2F</td><td>9</td></tr><tr><td>3</td><td>AK2F</td><td>AK2F</td><td>12</td></tr><tr><td>4</td><td>AK2F</td><td>AK2F</td><td>8/6 gal</td></tr><tr><td>5</td><td>AK2F</td><td>AK2F</td><td>8/6 GPD</td></tr><tr><td>6</td><td>AK2F</td><td>AK2F</td><td>7</td></tr><tr><td>7</td><td>AK2F</td><td>AK2F</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS ready mix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 2min<br>72.0°C : 2min<br>4.0°C : Infinate<br>X40 cycle</p><img src="https://static.igem.org/mediawiki/2017/0/08/IGKU_b2_034_0.jpeg">
| |
| − | <!-- IGKU_b2_034_1.jpeg -->
| |
| − | <!-- IGKU_b2_034_2.jpeg -->
| |
| − | <!-- IGKU_b2_034_3.jpeg -->
| |
| − | <!-- IGKU_b2_034_4.jpeg -->
| |
| − | <!-- IGKU_b2_034_5.jpeg -->
| |
| − | <!-- IGKU_b2_034_6.jpeg -->
| |
| − | <!-- IGKU_b2_034_7.jpeg -->
| |
| − | <!-- IGKU_b2_034_8.jpeg -->
| |
| − | <!-- IGKU_b2_034_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0813" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/13</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>4</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>5</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>7</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>7</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>9</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>8</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>11</td><td>12</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/3/31/IGKU_b2_035_0.jpeg">
| |
| − | <!-- IGKU_b2_035_1.jpeg -->
| |
| − | <!-- IGKU_b2_035_2.jpeg -->
| |
| − | <!-- IGKU_b2_035_3.jpeg -->
| |
| − | <!-- IGKU_b2_035_4.jpeg -->
| |
| − | <!-- IGKU_b2_035_5.jpeg -->
| |
| − | <!-- IGKU_b2_035_6.jpeg -->
| |
| − | <!-- IGKU_b2_035_7.jpeg -->
| |
| − | <!-- IGKU_b2_035_8.jpeg -->
| |
| − | <!-- IGKU_b2_035_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0813" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/13</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>14</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>16</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>22</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>24</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>26</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>30</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>36</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>24</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>14</td><td>5.0</td><td>Pst1</td><td>0.0</td><td>10xH</td><td>0.0</td><td>10.0</td></tr><tr><td>10</td><td>22</td><td>5.0</td><td>Pst1</td><td>0.0</td><td>10xH</td><td>0.0</td><td>10.0</td></tr><tr><td>11</td><td>36</td><td>5.0</td><td>Pst1</td><td>0.0</td><td>10xH</td><td>0.0</td><td>10.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/a/ad/IGKU_b2_036_0.jpeg">
| |
| − | <!-- IGKU_b2_036_1.jpeg -->
| |
| − | <!-- IGKU_b2_036_2.jpeg -->
| |
| − | <!-- IGKU_b2_036_3.jpeg -->
| |
| − | <!-- IGKU_b2_036_4.jpeg -->
| |
| − | <!-- IGKU_b2_036_5.jpeg -->
| |
| − | <!-- IGKU_b2_036_6.jpeg -->
| |
| − | <!-- IGKU_b2_036_7.jpeg -->
| |
| − | <!-- IGKU_b2_036_8.jpeg -->
| |
| − | <!-- IGKU_b2_036_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0813" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/13</h3></span>
| |
| − | <span class="keln_exp"><h4>colony PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>77-1</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>77-2</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>77-3</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>78-4</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>78-5</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS ready mix <br>95.0°C : 1min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min<br>72.0°C : 1min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_037_0.jpeg -->
| |
| − | <!-- IGKU_b2_037_1.jpeg -->
| |
| − | <!-- IGKU_b2_037_2.jpeg -->
| |
| − | <!-- IGKU_b2_037_3.jpeg -->
| |
| − | <!-- IGKU_b2_037_4.jpeg -->
| |
| − | <!-- IGKU_b2_037_5.jpeg -->
| |
| − | <!-- IGKU_b2_037_6.jpeg -->
| |
| − | <!-- IGKU_b2_037_7.jpeg -->
| |
| − | <!-- IGKU_b2_037_8.jpeg -->
| |
| − | <!-- IGKU_b2_037_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0813" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/13</h3></span>
| |
| − | <span class="keln_exp"><h4>Miniprep_Nanodrop</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Sample name</th><th>260/280</th><th>260/230</th><th>ng/ul</th></tr><tr><td>1</td><td>14</td><td>1.91</td><td>1.44</td><td>82.6</td></tr><tr><td>2</td><td>16</td><td>1.96</td><td>1.67</td><td>91.4</td></tr><tr><td>3</td><td>22</td><td>2.04</td><td>1.77</td><td>96.7</td></tr><tr><td>4</td><td>24</td><td>1.88</td><td>1.5</td><td>93.0</td></tr><tr><td>5</td><td>26</td><td>1.89</td><td>1.51</td><td>89.4</td></tr><tr><td>6</td><td>30</td><td>1.9</td><td>1.48</td><td>74.6</td></tr><tr><td>7</td><td>36</td><td>1.91</td><td>1.5</td><td>87.5</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_037_0.jpeg -->
| |
| − | <!-- IGKU_b1_037_1.jpeg -->
| |
| − | <!-- IGKU_b1_037_2.jpeg -->
| |
| − | <!-- IGKU_b1_037_3.jpeg -->
| |
| − | <!-- IGKU_b1_037_4.jpeg -->
| |
| − | <!-- IGKU_b1_037_5.jpeg -->
| |
| − | <!-- IGKU_b1_037_6.jpeg -->
| |
| − | <!-- IGKU_b1_037_7.jpeg -->
| |
| − | <!-- IGKU_b1_037_8.jpeg -->
| |
| − | <!-- IGKU_b1_037_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">The way of extraction : promega PureYield Plasmid Miniprepkit</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0813" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/13</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>1</td><td>Gal-AK2 99</td><td>2.0</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>1</td><td>GPD-AK2 80</td><td>2.0</td></tr><tr><td>3</td><td>YEplac195/sma1</td><td>1</td><td>Ter-AK2 81</td><td>2.0</td></tr><tr><td>4</td><td>YEplac195/sma1</td><td>1</td><td>T7 AK1 82</td><td>2.0</td></tr><tr><td>5</td><td>YEplac195/sma1</td><td>1</td><td>T7 AK1.2 83</td><td>2.0</td></tr><tr><td>6</td><td>YEplac195/sma1</td><td>1</td><td>Gal1 gfp 85</td><td>2.0</td></tr><tr><td>7</td><td>YEplac195/sma1</td><td>1</td><td>ter gfp 86</td><td>2.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_038_0.jpeg -->
| |
| − | <!-- IGKU_b1_038_1.jpeg -->
| |
| − | <!-- IGKU_b1_038_2.jpeg -->
| |
| − | <!-- IGKU_b1_038_3.jpeg -->
| |
| − | <!-- IGKU_b1_038_4.jpeg -->
| |
| − | <!-- IGKU_b1_038_5.jpeg -->
| |
| − | <!-- IGKU_b1_038_6.jpeg -->
| |
| − | <!-- IGKU_b1_038_7.jpeg -->
| |
| − | <!-- IGKU_b1_038_8.jpeg -->
| |
| − | <!-- IGKU_b1_038_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0813" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/13</h3></span>
| |
| − | <span class="keln_exp"><h4>Nebuilder</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>Sample</th><th>Vectorname</th><th>Vector amount (ul)</th><th> Insert1 name</th><th> Insert1 amount(ul)</th><th>Insert2 name </th><th>Insert2 amount (ul)</th><th>Mastermix amount (ul)</th><th>Total amount(ul)</th></tr><tr><td>1</td><td>YEPlac195/sma1</td><td>0.25</td><td>GPD-AK</td><td>1.5</td><td>Ter-AK</td><td>5.0</td><td>5.0</td><td>12.0</td></tr><tr><td>2</td><td>YEPlac195/sma1</td><td>0.25</td><td>Gal-AK</td><td>2.0</td><td>Ter-AK</td><td>5.0</td><td>5.0</td><td>12.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_038_0.jpeg -->
| |
| − | <!-- IGKU_b1_038_1.jpeg -->
| |
| − | <!-- IGKU_b1_038_2.jpeg -->
| |
| − | <!-- IGKU_b1_038_3.jpeg -->
| |
| − | <!-- IGKU_b1_038_4.jpeg -->
| |
| − | <!-- IGKU_b1_038_5.jpeg -->
| |
| − | <!-- IGKU_b1_038_6.jpeg -->
| |
| − | <!-- IGKU_b1_038_7.jpeg -->
| |
| − | <!-- IGKU_b1_038_8.jpeg -->
| |
| − | <!-- IGKU_b1_038_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0813" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/13</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>Nebuilder product</td></tr><tr><td>2</td><td>DH5a</td><td>Nebuilder product</td></tr><tr><td>3</td><td>DH5a</td><td>Nebuilder product</td></tr><tr><td>4</td><td>DH5a</td><td>Nebuilder product</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_039_0.jpeg -->
| |
| − | <!-- IGKU_b1_039_1.jpeg -->
| |
| − | <!-- IGKU_b1_039_2.jpeg -->
| |
| − | <!-- IGKU_b1_039_3.jpeg -->
| |
| − | <!-- IGKU_b1_039_4.jpeg -->
| |
| − | <!-- IGKU_b1_039_5.jpeg -->
| |
| − | <!-- IGKU_b1_039_6.jpeg -->
| |
| − | <!-- IGKU_b1_039_7.jpeg -->
| |
| − | <!-- IGKU_b1_039_8.jpeg -->
| |
| − | <!-- IGKU_b1_039_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0814" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/14</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>79-1</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>80-1</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>80-2</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>81-1</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>97-1</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>98-1</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>98-2</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>98-3</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>92-1</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>99-1</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>100-1</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>101-1</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>101-2</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>101-3</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>102-1</td></tr><tr><td>16</td><td>M13F</td><td>M13R</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS readymix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min20sec<br>72.0°C : 1min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/1/1f/IGKU_b2_038_0.jpeg"><img src="https://static.igem.org/mediawiki/2017/f/fe/IGKU_b2_038_1.jpeg">
| |
| − | <!-- IGKU_b2_038_2.jpeg -->
| |
| − | <!-- IGKU_b2_038_3.jpeg -->
| |
| − | <!-- IGKU_b2_038_4.jpeg -->
| |
| − | <!-- IGKU_b2_038_5.jpeg -->
| |
| − | <!-- IGKU_b2_038_6.jpeg -->
| |
| − | <!-- IGKU_b2_038_7.jpeg -->
| |
| − | <!-- IGKU_b2_038_8.jpeg -->
| |
| − | <!-- IGKU_b2_038_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0814" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/14</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>4</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>5</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>7</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>14</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>16</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>22</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>77-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>77-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>11</td><td>77-3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>12</td><td>24</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>13</td><td>26</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>14</td><td>30</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>15</td><td>36</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>16</td><td>78-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>17</td><td>78-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>18</td><td>78</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>19</td><td>99</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>20</td><td>100</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>21</td><td>101</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/b/bf/IGKU_b2_039_0.jpeg"><img src="https://static.igem.org/mediawiki/2017/c/cf/IGKU_b2_039_1.jpeg">
| |
| − | <!-- IGKU_b2_039_2.jpeg -->
| |
| − | <!-- IGKU_b2_039_3.jpeg -->
| |
| − | <!-- IGKU_b2_039_4.jpeg -->
| |
| − | <!-- IGKU_b2_039_5.jpeg -->
| |
| − | <!-- IGKU_b2_039_6.jpeg -->
| |
| − | <!-- IGKU_b2_039_7.jpeg -->
| |
| − | <!-- IGKU_b2_039_8.jpeg -->
| |
| − | <!-- IGKU_b2_039_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0814" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/14</h3></span>
| |
| − | <span class="keln_exp"><h4>colony_PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>97</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>98</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>99</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>100</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>101</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>102</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_040_0.jpeg -->
| |
| − | <!-- IGKU_b1_040_1.jpeg -->
| |
| − | <!-- IGKU_b1_040_2.jpeg -->
| |
| − | <!-- IGKU_b1_040_3.jpeg -->
| |
| − | <!-- IGKU_b1_040_4.jpeg -->
| |
| − | <!-- IGKU_b1_040_5.jpeg -->
| |
| − | <!-- IGKU_b1_040_6.jpeg -->
| |
| − | <!-- IGKU_b1_040_7.jpeg -->
| |
| − | <!-- IGKU_b1_040_8.jpeg -->
| |
| − | <!-- IGKU_b1_040_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS readymix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min20sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0814" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/14</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>Nebuilder product gal1-gfp</td></tr><tr><td>2</td><td>DH5a</td><td>Nebuilder product GPD-gfp</td></tr><tr><td>3</td><td>DH5a</td><td>8/13 ligation product Gal-AK2</td></tr><tr><td>4</td><td>DH5a</td><td>8/13 ligation product GPD-AK2</td></tr><tr><td>5</td><td>DH5a</td><td>8/13 ligation product Ter-AK2</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_040_0.jpeg -->
| |
| − | <!-- IGKU_b1_040_1.jpeg -->
| |
| − | <!-- IGKU_b1_040_2.jpeg -->
| |
| − | <!-- IGKU_b1_040_3.jpeg -->
| |
| − | <!-- IGKU_b1_040_4.jpeg -->
| |
| − | <!-- IGKU_b1_040_5.jpeg -->
| |
| − | <!-- IGKU_b1_040_6.jpeg -->
| |
| − | <!-- IGKU_b1_040_7.jpeg -->
| |
| − | <!-- IGKU_b1_040_8.jpeg -->
| |
| − | <!-- IGKU_b1_040_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0814" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/14</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>ligation product Gal-AK2</td></tr><tr><td>2</td><td>DH5a</td><td>ligation product GPD-AK2</td></tr><tr><td>3</td><td>DH5a</td><td>ligation product Ter-AK2</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_041_0.jpeg -->
| |
| − | <!-- IGKU_b1_041_1.jpeg -->
| |
| − | <!-- IGKU_b1_041_2.jpeg -->
| |
| − | <!-- IGKU_b1_041_3.jpeg -->
| |
| − | <!-- IGKU_b1_041_4.jpeg -->
| |
| − | <!-- IGKU_b1_041_5.jpeg -->
| |
| − | <!-- IGKU_b1_041_6.jpeg -->
| |
| − | <!-- IGKU_b1_041_7.jpeg -->
| |
| − | <!-- IGKU_b1_041_8.jpeg -->
| |
| − | <!-- IGKU_b1_041_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0814" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/14</h3></span>
| |
| − | <span class="keln_exp"><h4>Miniprep_Nanodrop</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Sample name</th><th>260/280</th><th>260/230</th><th>ng/ul</th></tr><tr><td>1</td><td>101</td><td>1.97</td><td>0.93</td><td>24.9</td></tr><tr><td>2</td><td>100</td><td>1.99</td><td>0.99</td><td>34.8</td></tr><tr><td>3</td><td>97</td><td>1.94</td><td>1.35</td><td>50.1</td></tr><tr><td>4</td><td>98</td><td>1.97</td><td>1.97</td><td>40.3</td></tr><tr><td>5</td><td>99.0</td><td>1.96</td><td>1.65</td><td>99.7</td></tr><tr><td>6</td><td>73.5</td><td>1.82</td><td>0.9</td><td>47.9</td></tr><tr><td>7</td><td>78.4</td><td>1.86</td><td>0.99</td><td>43.7</td></tr><tr><td>8</td><td>71-3</td><td>1.88</td><td>1.21</td><td>67.4</td></tr><tr><td>9</td><td>36</td><td>1.88</td><td>1.41</td><td>129.5</td></tr><tr><td>10</td><td>77-1</td><td>1.81</td><td>0.99</td><td>70.8</td></tr><tr><td>11</td><td>77-2</td><td>1.89</td><td>1.2</td><td>63.0</td></tr><tr><td>12</td><td>30</td><td>1.82</td><td>1.23</td><td>135.8</td></tr><tr><td>13</td><td>26</td><td>1.86</td><td>1.31</td><td>143.9</td></tr><tr><td>14</td><td>24</td><td>1.88</td><td>1.43</td><td>141.5</td></tr><tr><td>15</td><td>14</td><td>1.82</td><td>1.31</td><td>178.7</td></tr><tr><td>16</td><td>16</td><td>1.91</td><td>1.37</td><td>132.3</td></tr><tr><td>17</td><td>22</td><td>1.79</td><td>1.35</td><td>207.8</td></tr><tr><td>18</td><td>7</td><td>1.85</td><td>1.28</td><td>94.4</td></tr><tr><td>19</td><td>5</td><td>1.79</td><td>0.91</td><td>63.8</td></tr><tr><td>20</td><td>4</td><td>1.66</td><td>0.81</td><td>64.2</td></tr><tr><td>21</td><td>2</td><td>1.87</td><td>0.96</td><td>50.5</td></tr><tr><td>22</td><td>3</td><td>1.79</td><td>1.15</td><td>67.1</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_042_0.jpeg -->
| |
| − | <!-- IGKU_b1_042_1.jpeg -->
| |
| − | <!-- IGKU_b1_042_2.jpeg -->
| |
| − | <!-- IGKU_b1_042_3.jpeg -->
| |
| − | <!-- IGKU_b1_042_4.jpeg -->
| |
| − | <!-- IGKU_b1_042_5.jpeg -->
| |
| − | <!-- IGKU_b1_042_6.jpeg -->
| |
| − | <!-- IGKU_b1_042_7.jpeg -->
| |
| − | <!-- IGKU_b1_042_8.jpeg -->
| |
| − | <!-- IGKU_b1_042_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">The way of extraction : promega</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0815" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/15</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>92</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>97</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>98</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>100</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>101</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>92</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>97</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>98</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>100</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>101</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/2/26/IGKU_b2_040_0.jpeg">
| |
| − | <!-- IGKU_b2_040_1.jpeg -->
| |
| − | <!-- IGKU_b2_040_2.jpeg -->
| |
| − | <!-- IGKU_b2_040_3.jpeg -->
| |
| − | <!-- IGKU_b2_040_4.jpeg -->
| |
| − | <!-- IGKU_b2_040_5.jpeg -->
| |
| − | <!-- IGKU_b2_040_6.jpeg -->
| |
| − | <!-- IGKU_b2_040_7.jpeg -->
| |
| − | <!-- IGKU_b2_040_8.jpeg -->
| |
| − | <!-- IGKU_b2_040_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0815" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/15</h3></span>
| |
| − | <span class="keln_exp"><h4>Nebuilder</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>Sample</th><th>Vectorname</th><th>Vector amount (ul)</th><th> Insert1 name</th><th> Insert1 amount(ul)</th><th>Insert2 name </th><th>Insert2 amount (ul)</th><th>Mastermix amount (ul)</th><th>Total amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>0.25</td><td>Gal-AK</td><td>1.825</td><td>Ter-AK</td><td>1.5</td><td>5.0</td><td>10.0</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>0.25</td><td>GPD-AK</td><td>1.25</td><td>Ter-AK</td><td>1.5</td><td>5.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_043_0.jpeg -->
| |
| − | <!-- IGKU_b1_043_1.jpeg -->
| |
| − | <!-- IGKU_b1_043_2.jpeg -->
| |
| − | <!-- IGKU_b1_043_3.jpeg -->
| |
| − | <!-- IGKU_b1_043_4.jpeg -->
| |
| − | <!-- IGKU_b1_043_5.jpeg -->
| |
| − | <!-- IGKU_b1_043_6.jpeg -->
| |
| − | <!-- IGKU_b1_043_7.jpeg -->
| |
| − | <!-- IGKU_b1_043_8.jpeg -->
| |
| − | <!-- IGKU_b1_043_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0815" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/15</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>DH5a</td><td>111</td></tr><tr><td>2</td><td>DH5a</td><td>112</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_043_0.jpeg -->
| |
| − | <!-- IGKU_b1_043_1.jpeg -->
| |
| − | <!-- IGKU_b1_043_2.jpeg -->
| |
| − | <!-- IGKU_b1_043_3.jpeg -->
| |
| − | <!-- IGKU_b1_043_4.jpeg -->
| |
| − | <!-- IGKU_b1_043_5.jpeg -->
| |
| − | <!-- IGKU_b1_043_6.jpeg -->
| |
| − | <!-- IGKU_b1_043_7.jpeg -->
| |
| − | <!-- IGKU_b1_043_8.jpeg -->
| |
| − | <!-- IGKU_b1_043_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0817" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/17</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK25</td><td>IK26</td><td>Rev</td></tr><tr><td>2</td><td>IK27</td><td>IK22</td><td>yeast genome</td></tr><tr><td>3</td><td>IK23</td><td>IK28</td><td>yeast genome</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS Ready mix <br>98.0°C : 3min<br>98.0°C : 20sec<br>62.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/c/ce/IGKU_b2_041_0.jpeg">
| |
| − | <!-- IGKU_b2_041_1.jpeg -->
| |
| − | <!-- IGKU_b2_041_2.jpeg -->
| |
| − | <!-- IGKU_b2_041_3.jpeg -->
| |
| − | <!-- IGKU_b2_041_4.jpeg -->
| |
| − | <!-- IGKU_b2_041_5.jpeg -->
| |
| − | <!-- IGKU_b2_041_6.jpeg -->
| |
| − | <!-- IGKU_b2_041_7.jpeg -->
| |
| − | <!-- IGKU_b2_041_8.jpeg -->
| |
| − | <!-- IGKU_b2_041_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0817" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/17</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK27</td><td>IK22</td><td>yeast genome</td></tr><tr><td>2</td><td>IK23</td><td>IK28</td><td>yeast genome</td></tr><tr><td>3</td><td>IK27</td><td>IK28</td><td>GPDp+Rev+GPDt</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS ready mix <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/9/96/IGKU_b2_042_0.jpeg">
| |
| − | <!-- IGKU_b2_042_1.jpeg -->
| |
| − | <!-- IGKU_b2_042_2.jpeg -->
| |
| − | <!-- IGKU_b2_042_3.jpeg -->
| |
| − | <!-- IGKU_b2_042_4.jpeg -->
| |
| − | <!-- IGKU_b2_042_5.jpeg -->
| |
| − | <!-- IGKU_b2_042_6.jpeg -->
| |
| − | <!-- IGKU_b2_042_7.jpeg -->
| |
| − | <!-- IGKU_b2_042_8.jpeg -->
| |
| − | <!-- IGKU_b2_042_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0817" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/17</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK25</td><td>IK26</td><td>Rev</td></tr><tr><td>2</td><td>IK27</td><td>IK22</td><td>yeast genome</td></tr><tr><td>3</td><td>IK23</td><td>IK28</td><td>yeast genome</td></tr><tr><td>4</td><td>IK25</td><td>IK26</td><td>Rev after purification</td></tr><tr><td>5</td><td>IK27</td><td>IK22</td><td>GPDp after purification</td></tr><tr><td>6</td><td>IK23</td><td>IK28</td><td>GPDt after purification</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS ready mix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X36 cycle</p><img src="https://static.igem.org/mediawiki/2017/b/bf/IGKU_b2_043_0.jpeg">
| |
| − | <!-- IGKU_b2_043_1.jpeg -->
| |
| − | <!-- IGKU_b2_043_2.jpeg -->
| |
| − | <!-- IGKU_b2_043_3.jpeg -->
| |
| − | <!-- IGKU_b2_043_4.jpeg -->
| |
| − | <!-- IGKU_b2_043_5.jpeg -->
| |
| − | <!-- IGKU_b2_043_6.jpeg -->
| |
| − | <!-- IGKU_b2_043_7.jpeg -->
| |
| − | <!-- IGKU_b2_043_8.jpeg -->
| |
| − | <!-- IGKU_b2_043_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0817" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/17</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>86-1</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>86-2</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>81-1</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>81-2</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>81-3</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>95-1</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>95-2</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>95-3</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>95-4</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>93-1</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>93-2</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>92</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>brue colony</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>11</td></tr>
| |
| − | </table>
| |
| − | <img src="http://https://static.igem.org/mediawiki/2017/4/48/IGKU_b1_045_0.jpeg">
| |
| − | <!-- IGKU_b1_045_1.jpeg -->
| |
| − | <!-- IGKU_b1_045_2.jpeg -->
| |
| − | <!-- IGKU_b1_045_3.jpeg -->
| |
| − | <!-- IGKU_b1_045_4.jpeg -->
| |
| − | <!-- IGKU_b1_045_5.jpeg -->
| |
| − | <!-- IGKU_b1_045_6.jpeg -->
| |
| − | <!-- IGKU_b1_045_7.jpeg -->
| |
| − | <!-- IGKU_b1_045_8.jpeg -->
| |
| − | <!-- IGKU_b1_045_9.jpeg -->
| |
| − | </div>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS readymix <br>95.0°C : 3min<br>95.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min20sec<br>72.0°C : 1min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0817" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/17</h3></span>
| |
| − | <span class="keln_exp"><h4>Nebuilder</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>Sample</th><th>Vectorname</th><th>Vector amount (ul)</th><th> Insert1 name</th><th> Insert1 amount(ul)</th><th>Insert2 name </th><th>Insert2 amount (ul)</th><th>Mastermix amount (ul)</th><th>Total amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>0.8</td><td>Gal-AK2</td><td>6.5</td><td>Ter-AK2</td><td>5.0</td><td>10.0</td><td>22.3</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>0.8</td><td>GPD-AK2</td><td>4.2</td><td>Ter-AK2</td><td>5.0</td><td>10.0</td><td>20.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_046_0.jpeg -->
| |
| − | <!-- IGKU_b1_046_1.jpeg -->
| |
| − | <!-- IGKU_b1_046_2.jpeg -->
| |
| − | <!-- IGKU_b1_046_3.jpeg -->
| |
| − | <!-- IGKU_b1_046_4.jpeg -->
| |
| − | <!-- IGKU_b1_046_5.jpeg -->
| |
| − | <!-- IGKU_b1_046_6.jpeg -->
| |
| − | <!-- IGKU_b1_046_7.jpeg -->
| |
| − | <!-- IGKU_b1_046_8.jpeg -->
| |
| − | <!-- IGKU_b1_046_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0817" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/17</h3></span>
| |
| − | <span class="keln_exp"><h4>Transformation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>CompetentCell name</th><th>DNA name</th></tr><tr><td>1</td><td>BL21</td><td>113</td></tr><tr><td>2</td><td>BL21</td><td>114</td></tr><tr><td>3</td><td>BL21</td><td>115</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b1_046_0.jpeg -->
| |
| − | <!-- IGKU_b1_046_1.jpeg -->
| |
| − | <!-- IGKU_b1_046_2.jpeg -->
| |
| − | <!-- IGKU_b1_046_3.jpeg -->
| |
| − | <!-- IGKU_b1_046_4.jpeg -->
| |
| − | <!-- IGKU_b1_046_5.jpeg -->
| |
| − | <!-- IGKU_b1_046_6.jpeg -->
| |
| − | <!-- IGKU_b1_046_7.jpeg -->
| |
| − | <!-- IGKU_b1_046_8.jpeg -->
| |
| − | <!-- IGKU_b1_046_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0818" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/18</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>79-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>79-3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>79-6</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>80-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>80-7</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>80-13</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>81-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>81-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>97</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>99-A</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>11</td><td>99-B</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>12</td><td>100</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>13</td><td>100-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>14</td><td>100-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>15</td><td>101-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>16</td><td>101 3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>17</td><td>102</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>None</td><td>None</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/1/12/IGKU_b2_044_0.jpeg"><img src="https://static.igem.org/mediawiki/2017/e/e1/IGKU_b2_044_1.jpeg"><img src="https://static.igem.org/mediawiki/2017/b/b3/IGKU_b2_044_2.jpeg">
| |
| − | <!-- IGKU_b2_044_3.jpeg -->
| |
| − | <!-- IGKU_b2_044_4.jpeg -->
| |
| − | <!-- IGKU_b2_044_5.jpeg -->
| |
| − | <!-- IGKU_b2_044_6.jpeg -->
| |
| − | <!-- IGKU_b2_044_7.jpeg -->
| |
| − | <!-- IGKU_b2_044_8.jpeg -->
| |
| − | <!-- IGKU_b2_044_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0818" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/18</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK27</td><td>IK28</td><td>Rev</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS Ready mix <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 20sec<br>72.0°C : 1min20sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/1/16/IGKU_b2_045_0.jpeg">
| |
| − | <!-- IGKU_b2_045_1.jpeg -->
| |
| − | <!-- IGKU_b2_045_2.jpeg -->
| |
| − | <!-- IGKU_b2_045_3.jpeg -->
| |
| − | <!-- IGKU_b2_045_4.jpeg -->
| |
| − | <!-- IGKU_b2_045_5.jpeg -->
| |
| − | <!-- IGKU_b2_045_6.jpeg -->
| |
| − | <!-- IGKU_b2_045_7.jpeg -->
| |
| − | <!-- IGKU_b2_045_8.jpeg -->
| |
| − | <!-- IGKU_b2_045_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0818" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/18</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>AK2R</td><td>79-2</td></tr><tr><td>2</td><td>M13F</td><td>AK2R</td><td>79-3</td></tr><tr><td>3</td><td>M13F</td><td>AK2R</td><td>80-13</td></tr><tr><td>4</td><td>M13F</td><td>AK2F</td><td>79-2</td></tr><tr><td>5</td><td>M13F</td><td>AK2F</td><td>79-3</td></tr><tr><td>6</td><td>M13F</td><td>AK2F</td><td>80-13</td></tr><tr><td>7</td><td>M13R</td><td>M13F</td><td>blue colony</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS readymix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min30sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_046_0.jpeg -->
| |
| − | <!-- IGKU_b2_046_1.jpeg -->
| |
| − | <!-- IGKU_b2_046_2.jpeg -->
| |
| − | <!-- IGKU_b2_046_3.jpeg -->
| |
| − | <!-- IGKU_b2_046_4.jpeg -->
| |
| − | <!-- IGKU_b2_046_5.jpeg -->
| |
| − | <!-- IGKU_b2_046_6.jpeg -->
| |
| − | <!-- IGKU_b2_046_7.jpeg -->
| |
| − | <!-- IGKU_b2_046_8.jpeg -->
| |
| − | <!-- IGKU_b2_046_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0818" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/18</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK22</td><td>IK27</td><td>GPDp after purification</td></tr><tr><td>2</td><td>IK22</td><td>IK27</td><td>GPDp after purification</td></tr><tr><td>3</td><td>IK22</td><td>IK27</td><td>GPDp after purification</td></tr><tr><td>4</td><td>IK22</td><td>IK27</td><td>GPDp after purification</td></tr><tr><td>5</td><td>M13F</td><td>AK2R</td><td>113gal</td></tr><tr><td>6</td><td>M13F</td><td>AK2R</td><td>114GPD</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS readymix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min30sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: lane1~4<br>Gel extraction: lane1~4</p><img src="https://static.igem.org/mediawiki/2017/4/4c/IGKU_b2_047_0.jpeg">
| |
| − | <!-- IGKU_b2_047_1.jpeg -->
| |
| − | <!-- IGKU_b2_047_2.jpeg -->
| |
| − | <!-- IGKU_b2_047_3.jpeg -->
| |
| − | <!-- IGKU_b2_047_4.jpeg -->
| |
| − | <!-- IGKU_b2_047_5.jpeg -->
| |
| − | <!-- IGKU_b2_047_6.jpeg -->
| |
| − | <!-- IGKU_b2_047_7.jpeg -->
| |
| − | <!-- IGKU_b2_047_8.jpeg -->
| |
| − | <!-- IGKU_b2_047_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0819" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/19</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>141</td><td>0.34</td><td>2T7akRRE3'</td><td>9.4</td></tr><tr><td>2</td><td>142</td><td>0.34</td><td>2T7ak1stem</td><td>8.3</td></tr><tr><td>3</td><td>143</td><td>0.34</td><td>1T7ak1stem</td><td>4.4</td></tr><tr><td>4</td><td>144</td><td>0.34</td><td>2T7RREak1</td><td>5.3</td></tr><tr><td>5</td><td>145</td><td>0.34</td><td>1T7RREak1</td><td>8.5</td></tr><tr><td>6</td><td>146</td><td>0.34</td><td>GPD+AK2</td><td>13.2</td></tr><tr><td>7</td><td>147</td><td>0.34</td><td>Gal1+AK2</td><td>15.8</td></tr><tr><td>8</td><td>148</td><td>0.34</td><td>AK2+ter</td><td>20.1</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_048_0.jpeg -->
| |
| − | <!-- IGKU_b2_048_1.jpeg -->
| |
| − | <!-- IGKU_b2_048_2.jpeg -->
| |
| − | <!-- IGKU_b2_048_3.jpeg -->
| |
| − | <!-- IGKU_b2_048_4.jpeg -->
| |
| − | <!-- IGKU_b2_048_5.jpeg -->
| |
| − | <!-- IGKU_b2_048_6.jpeg -->
| |
| − | <!-- IGKU_b2_048_7.jpeg -->
| |
| − | <!-- IGKU_b2_048_8.jpeg -->
| |
| − | <!-- IGKU_b2_048_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0820" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/20</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK22</td><td>IK27</td><td>GPDp</td></tr><tr><td>2</td><td>IK22</td><td>IK27</td><td>GPDp</td></tr><tr><td>3</td><td>IK22</td><td>IK27</td><td>yeast genome</td></tr><tr><td>4</td><td>IK22</td><td>IK27</td><td>yeast genome</td></tr><tr><td>5</td><td>IK22</td><td>IK27</td><td>yeast genome</td></tr><tr><td>6</td><td>IK22</td><td>IK27</td><td>yeast genome</td></tr><tr><td>7</td><td>IK27</td><td>IK28</td><td>GPDp+Rev+GPDt</td></tr><tr><td>8</td><td>IK27</td><td>IK28</td><td>GPDp+Rev+GPDt</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS ready mix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min30sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_050_0.jpeg -->
| |
| − | <!-- IGKU_b2_050_1.jpeg -->
| |
| − | <!-- IGKU_b2_050_2.jpeg -->
| |
| − | <!-- IGKU_b2_050_3.jpeg -->
| |
| − | <!-- IGKU_b2_050_4.jpeg -->
| |
| − | <!-- IGKU_b2_050_5.jpeg -->
| |
| − | <!-- IGKU_b2_050_6.jpeg -->
| |
| − | <!-- IGKU_b2_050_7.jpeg -->
| |
| − | <!-- IGKU_b2_050_8.jpeg -->
| |
| − | <!-- IGKU_b2_050_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0820" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/20</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK27</td><td>IK22</td><td>GPDp</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA HS readymix <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/8/88/IGKU_b2_052_0.jpeg">
| |
| − | <!-- IGKU_b2_052_1.jpeg -->
| |
| − | <!-- IGKU_b2_052_2.jpeg -->
| |
| − | <!-- IGKU_b2_052_3.jpeg -->
| |
| − | <!-- IGKU_b2_052_4.jpeg -->
| |
| − | <!-- IGKU_b2_052_5.jpeg -->
| |
| − | <!-- IGKU_b2_052_6.jpeg -->
| |
| − | <!-- IGKU_b2_052_7.jpeg -->
| |
| − | <!-- IGKU_b2_052_8.jpeg -->
| |
| − | <!-- IGKU_b2_052_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0821" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/21</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>2</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>3</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>4</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>5</td><td>M13R</td><td>AK1R</td><td>141 1-1</td></tr><tr><td>6</td><td>M13R</td><td>AK1R</td><td>141 1-2</td></tr><tr><td>7</td><td>M13R</td><td>AK1R</td><td>141 1-3</td></tr><tr><td>8</td><td>M13R</td><td>AK1R</td><td>141 1-4</td></tr><tr><td>9</td><td>M13R</td><td>AK1R</td><td>142 1-1</td></tr><tr><td>10</td><td>M13R</td><td>AK1R</td><td>142 1-2</td></tr><tr><td>11</td><td>M13R</td><td>AK1R</td><td>142 1-3</td></tr><tr><td>12</td><td>M13R</td><td>AK1R</td><td>142 1-4</td></tr><tr><td>13</td><td>M13R</td><td>AK1F</td><td>141 1-1</td></tr><tr><td>14</td><td>M13R</td><td>AK1F</td><td>141 1-2</td></tr><tr><td>15</td><td>M13R</td><td>AK1F</td><td>141 1-3</td></tr><tr><td>16</td><td>M13R</td><td>AK1F</td><td>141 1-4</td></tr><tr><td>17</td><td>M13R</td><td>AK1F</td><td>142 1-1</td></tr><tr><td>18</td><td>M13R</td><td>AK1F</td><td>142 1-2</td></tr><tr><td>19</td><td>M13R</td><td>AK1F</td><td>142 1-3</td></tr><tr><td>20</td><td>M13R</td><td>AK1F</td><td>142 1-4</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS Readymix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min20sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/0/00/IGKU_b2_055_0.jpeg">
| |
| − | <!-- IGKU_b2_055_1.jpeg -->
| |
| − | <!-- IGKU_b2_055_2.jpeg -->
| |
| − | <!-- IGKU_b2_055_3.jpeg -->
| |
| − | <!-- IGKU_b2_055_4.jpeg -->
| |
| − | <!-- IGKU_b2_055_5.jpeg -->
| |
| − | <!-- IGKU_b2_055_6.jpeg -->
| |
| − | <!-- IGKU_b2_055_7.jpeg -->
| |
| − | <!-- IGKU_b2_055_8.jpeg -->
| |
| − | <!-- IGKU_b2_055_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0821" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/21</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>AK1R</td><td>141 2-1</td></tr><tr><td>2</td><td>M13F</td><td>AK1R</td><td>141 2-4</td></tr><tr><td>3</td><td>M13F</td><td>AK1R</td><td>141 1-15</td></tr><tr><td>4</td><td>M13F</td><td>AK1R</td><td>142 1-5</td></tr><tr><td>5</td><td>M13F</td><td>AK1R</td><td>143 9</td></tr><tr><td>6</td><td>M13F</td><td>AK1R</td><td>143 13</td></tr><tr><td>7</td><td>M13F</td><td>AK1R</td><td>143 27</td></tr><tr><td>8</td><td>M13F</td><td>AK1R</td><td>143 2</td></tr><tr><td>9</td><td>M13F</td><td>AK1F</td><td>141 2-1</td></tr><tr><td>10</td><td>M13F</td><td>AK1F</td><td>141 2-4</td></tr><tr><td>11</td><td>M13F</td><td>AK1F</td><td>141 1-15</td></tr><tr><td>12</td><td>M13F</td><td>AK1F</td><td>142 1-5</td></tr><tr><td>13</td><td>M13F</td><td>AK1F</td><td>143 9</td></tr><tr><td>14</td><td>M13F</td><td>AK1F</td><td>143 13</td></tr><tr><td>15</td><td>M13F</td><td>AK1F</td><td>143 27</td></tr><tr><td>16</td><td>M13F</td><td>AK1F</td><td>143 2</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS Readymix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min20sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/c/cf/IGKU_b2_056_0.jpeg">
| |
| − | <!-- IGKU_b2_056_1.jpeg -->
| |
| − | <!-- IGKU_b2_056_2.jpeg -->
| |
| − | <!-- IGKU_b2_056_3.jpeg -->
| |
| − | <!-- IGKU_b2_056_4.jpeg -->
| |
| − | <!-- IGKU_b2_056_5.jpeg -->
| |
| − | <!-- IGKU_b2_056_6.jpeg -->
| |
| − | <!-- IGKU_b2_056_7.jpeg -->
| |
| − | <!-- IGKU_b2_056_8.jpeg -->
| |
| − | <!-- IGKU_b2_056_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0821" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/21</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>2</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>3</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>4</td><td>IK29</td><td>IK30</td><td>Rev</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS Readymix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min20sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: lane2~4<br>Gel extraction: lane2~4</p><img src="https://static.igem.org/mediawiki/2017/d/de/IGKU_b2_057_0.jpeg">
| |
| − | <!-- IGKU_b2_057_1.jpeg -->
| |
| − | <!-- IGKU_b2_057_2.jpeg -->
| |
| − | <!-- IGKU_b2_057_3.jpeg -->
| |
| − | <!-- IGKU_b2_057_4.jpeg -->
| |
| − | <!-- IGKU_b2_057_5.jpeg -->
| |
| − | <!-- IGKU_b2_057_6.jpeg -->
| |
| − | <!-- IGKU_b2_057_7.jpeg -->
| |
| − | <!-- IGKU_b2_057_8.jpeg -->
| |
| − | <!-- IGKU_b2_057_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0822" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>4</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>5</td><td>IK21</td><td>IK22</td><td>water</td></tr><tr><td>6</td><td>IK23</td><td>IK28</td><td>GPDt</td></tr><tr><td>7</td><td>IK23</td><td>IK28</td><td>GPDt</td></tr><tr><td>8</td><td>IK23</td><td>IK28</td><td>GPDt</td></tr><tr><td>9</td><td>IK23</td><td>IK28</td><td>GPDt</td></tr><tr><td>10</td><td>IK23</td><td>IK28</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS Readymix <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: lane2~4<br>Gel extraction: lane2~4</p><img src="https://static.igem.org/mediawiki/2017/9/9a/IGKU_b2_058_0.jpeg">
| |
| − | <!-- IGKU_b2_058_1.jpeg -->
| |
| − | <!-- IGKU_b2_058_2.jpeg -->
| |
| − | <!-- IGKU_b2_058_3.jpeg -->
| |
| − | <!-- IGKU_b2_058_4.jpeg -->
| |
| − | <!-- IGKU_b2_058_5.jpeg -->
| |
| − | <!-- IGKU_b2_058_6.jpeg -->
| |
| − | <!-- IGKU_b2_058_7.jpeg -->
| |
| − | <!-- IGKU_b2_058_8.jpeg -->
| |
| − | <!-- IGKU_b2_058_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0822" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK27</td><td>IK28</td><td>GPDp+Rev+GPDt</td></tr><tr><td>2</td><td>IK27</td><td>IK28</td><td>GPDp+Rev+GPDt</td></tr><tr><td>3</td><td>IK23</td><td>IK24</td><td>yeast genome</td></tr><tr><td>4</td><td>IK23</td><td>IK24</td><td>yeast genome</td></tr><tr><td>5</td><td>IK23</td><td>IK24</td><td>yeast genome</td></tr><tr><td>6</td><td>IK23</td><td>IK24</td><td>yeast genome</td></tr><tr><td>7</td><td>IK21</td><td>IK24</td><td>GPDp+Rev+GPDt</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS ReadyMIx <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min30sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/1/1a/IGKU_b2_059_0.jpeg">
| |
| − | <!-- IGKU_b2_059_1.jpeg -->
| |
| − | <!-- IGKU_b2_059_2.jpeg -->
| |
| − | <!-- IGKU_b2_059_3.jpeg -->
| |
| − | <!-- IGKU_b2_059_4.jpeg -->
| |
| − | <!-- IGKU_b2_059_5.jpeg -->
| |
| − | <!-- IGKU_b2_059_6.jpeg -->
| |
| − | <!-- IGKU_b2_059_7.jpeg -->
| |
| − | <!-- IGKU_b2_059_8.jpeg -->
| |
| − | <!-- IGKU_b2_059_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0822" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Fukuda</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>144 11</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>144 3</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>144 7</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>144 1</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>144 17</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>144 5</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>144 9</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>144 13</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>142 1</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>142 5</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>142 3</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>142 4</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>142 2-1</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>142 2-2</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>142 2-3</td></tr><tr><td>16</td><td>M13F</td><td>M13R</td><td>142 2-4</td></tr><tr><td>17</td><td>M13F</td><td>M13R</td><td>143 13</td></tr><tr><td>18</td><td>M13F</td><td>M13R</td><td>143 15</td></tr><tr><td>19</td><td>M13F</td><td>M13R</td><td>143 5</td></tr><tr><td>20</td><td>M13F</td><td>M13R</td><td>153 21</td></tr><tr><td>21</td><td>M13F</td><td>M13R</td><td>143 17</td></tr><tr><td>22</td><td>M13F</td><td>M13R</td><td>143 19</td></tr><tr><td>23</td><td>M13F</td><td>M13R</td><td>143 25</td></tr><tr><td>24</td><td>M13F</td><td>M13R</td><td>143 9</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS ReadyMix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min30sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_060_0.jpeg -->
| |
| − | <!-- IGKU_b2_060_1.jpeg -->
| |
| − | <!-- IGKU_b2_060_2.jpeg -->
| |
| − | <!-- IGKU_b2_060_3.jpeg -->
| |
| − | <!-- IGKU_b2_060_4.jpeg -->
| |
| − | <!-- IGKU_b2_060_5.jpeg -->
| |
| − | <!-- IGKU_b2_060_6.jpeg -->
| |
| − | <!-- IGKU_b2_060_7.jpeg -->
| |
| − | <!-- IGKU_b2_060_8.jpeg -->
| |
| − | <!-- IGKU_b2_060_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0822" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Takimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>145 1-13</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>145 2-13</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>145 2-15</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>145 1-5</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>145 2-17</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>145 2-11</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>145 2-9</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>145 1-17</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>146 2-3</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>146 2-1</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>146 1-3</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>146 2-7</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>146 2-9</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>146 2-5</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>146 1-4</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS ReadyMix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min30sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_061_0.jpeg -->
| |
| − | <!-- IGKU_b2_061_1.jpeg -->
| |
| − | <!-- IGKU_b2_061_2.jpeg -->
| |
| − | <!-- IGKU_b2_061_3.jpeg -->
| |
| − | <!-- IGKU_b2_061_4.jpeg -->
| |
| − | <!-- IGKU_b2_061_5.jpeg -->
| |
| − | <!-- IGKU_b2_061_6.jpeg -->
| |
| − | <!-- IGKU_b2_061_7.jpeg -->
| |
| − | <!-- IGKU_b2_061_8.jpeg -->
| |
| − | <!-- IGKU_b2_061_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0822" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/22</h3></span>
| |
| − | <span class="keln_exp"><h4>Nebuilder</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>Sample</th><th>Vectorname</th><th>Vector amount (ul)</th><th> Insert1 name</th><th> Insert1 amount(ul)</th><th>Insert2 name </th><th>Insert2 amount (ul)</th><th>Mastermix amount (ul)</th><th>Total amount(ul)</th></tr><tr><td>1</td><td>171.0</td><td>Vector amount (ul)</td><td>Gal1+gfp-2</td><td>0.78</td><td>Gal1+gfp</td><td>1.03</td><td>10.0</td><td>Total amount(ul)</td></tr><tr><td>2</td><td>172.0</td><td>Vector amount (ul)</td><td>Gal1+AK2</td><td>1.26</td><td>Gal1+AK2-2</td><td>1.01</td><td>10.0</td><td>Total amount(ul)</td></tr><tr><td>3</td><td>173.0</td><td>Vector amount (ul)</td><td>164.0</td><td>0.65</td><td>165.0</td><td>0.57</td><td>10.0</td><td>Total amount(ul)</td></tr><tr><td>4</td><td>174.0</td><td>Vector amount (ul)</td><td>161.0</td><td>0.65</td><td>162.0</td><td>0.76</td><td>10.0</td><td>Total amount(ul)</td></tr><tr><td>5</td><td>175.0</td><td>Vector amount (ul)</td><td>161.0</td><td>0.38</td><td>167.0</td><td>0.83</td><td>10.0</td><td>Total amount(ul)</td></tr><tr><td>6</td><td>176.0</td><td>Vector amount (ul)</td><td>166.0</td><td>0.38</td><td>165.0</td><td>0.83</td><td>10.0</td><td>Total amount(ul)</td></tr><tr><td>7</td><td>177.0</td><td>Vector amount (ul)</td><td>163.0</td><td>0.38</td><td>162.0</td><td>0.83</td><td>10.0</td><td>Total amount(ul)</td></tr><tr><td>8</td><td>178.0</td><td>Vector amount (ul)</td><td>163.0</td><td>0.38</td><td>167.0</td><td>0.83</td><td>10.0</td><td>Total amount(ul)</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_066_0.jpeg -->
| |
| − | <!-- IGKU_b2_066_1.jpeg -->
| |
| − | <!-- IGKU_b2_066_2.jpeg -->
| |
| − | <!-- IGKU_b2_066_3.jpeg -->
| |
| − | <!-- IGKU_b2_066_4.jpeg -->
| |
| − | <!-- IGKU_b2_066_5.jpeg -->
| |
| − | <!-- IGKU_b2_066_6.jpeg -->
| |
| − | <!-- IGKU_b2_066_7.jpeg -->
| |
| − | <!-- IGKU_b2_066_8.jpeg -->
| |
| − | <!-- IGKU_b2_066_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0822" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>None</td><td>None</td><td>GPDp+Rev+GPDt</td></tr><tr><td>2</td><td>IK27</td><td>IK28</td><td>lane1 after 15 cycle</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi Hs ReadyMix <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min30sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_067_0.jpeg -->
| |
| − | <!-- IGKU_b2_067_1.jpeg -->
| |
| − | <!-- IGKU_b2_067_2.jpeg -->
| |
| − | <!-- IGKU_b2_067_3.jpeg -->
| |
| − | <!-- IGKU_b2_067_4.jpeg -->
| |
| − | <!-- IGKU_b2_067_5.jpeg -->
| |
| − | <!-- IGKU_b2_067_6.jpeg -->
| |
| − | <!-- IGKU_b2_067_7.jpeg -->
| |
| − | <!-- IGKU_b2_067_8.jpeg -->
| |
| − | <!-- IGKU_b2_067_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0822" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK23</td><td>IK28</td><td>GPDt</td></tr><tr><td>2</td><td>IK23</td><td>IK28</td><td>GPDt</td></tr><tr><td>3</td><td>IK23</td><td>IK28</td><td>GPDt</td></tr><tr><td>4</td><td>IK23</td><td>IK28</td><td>GPDt</td></tr><tr><td>5</td><td>IK27</td><td>IK30</td><td>GPDp + Rev</td></tr><tr><td>6</td><td>IK21</td><td>IK30</td><td>GPDp + Rev</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi Robust <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/d/d2/IGKU_b2_069_0.jpeg">
| |
| − | <!-- IGKU_b2_069_1.jpeg -->
| |
| − | <!-- IGKU_b2_069_2.jpeg -->
| |
| − | <!-- IGKU_b2_069_3.jpeg -->
| |
| − | <!-- IGKU_b2_069_4.jpeg -->
| |
| − | <!-- IGKU_b2_069_5.jpeg -->
| |
| − | <!-- IGKU_b2_069_6.jpeg -->
| |
| − | <!-- IGKU_b2_069_7.jpeg -->
| |
| − | <!-- IGKU_b2_069_8.jpeg -->
| |
| − | <!-- IGKU_b2_069_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0823" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/23</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>147 2-17</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>147 1-2</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>147 2-3</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>147 7</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>141 1-1</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>141 1-2</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>141 1-4</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>148 2-3</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>148 2-5</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>148 2-4</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>148 1-5</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>148 1-3</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>144 11</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>144 3</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>144 1</td></tr><tr><td>16</td><td>M13F</td><td>M13R</td><td>146 1-6</td></tr><tr><td>17</td><td>M13F</td><td>M13R</td><td>146 1-4</td></tr><tr><td>18</td><td>M13F</td><td>M13R</td><td>146 2-5</td></tr><tr><td>19</td><td>M13F</td><td>M13R</td><td>146 2-7</td></tr><tr><td>20</td><td>M13F</td><td>M13R</td><td>142 2-2</td></tr><tr><td>21</td><td>M13F</td><td>M13R</td><td>142 1-1</td></tr><tr><td>22</td><td>M13F</td><td>M13R</td><td>142 2-7</td></tr><tr><td>23</td><td>M13F</td><td>M13R</td><td>142 2-3</td></tr><tr><td>24</td><td>M13F</td><td>M13R</td><td>145 2-11</td></tr><tr><td>25</td><td>M13F</td><td>M13R</td><td>145 1-17</td></tr><tr><td>26</td><td>M13F</td><td>M13R</td><td>145 2-9</td></tr><tr><td>27</td><td>M13F</td><td>M13R</td><td>145 2-17</td></tr><tr><td>28</td><td>M13F</td><td>M13R</td><td>143 5</td></tr><tr><td>29</td><td>M13F</td><td>M13R</td><td>143 17</td></tr><tr><td>30</td><td>M13F</td><td>M13R</td><td>143 15</td></tr><tr><td>31</td><td>M13F</td><td>M13R</td><td>143 21</td></tr><tr><td>32</td><td>M13F</td><td>AK1R</td><td>147 2-17</td></tr><tr><td>33</td><td>M13F</td><td>AK1R</td><td>147 1-2</td></tr><tr><td>34</td><td>M13F</td><td>AK1R</td><td>147 2-3</td></tr><tr><td>35</td><td>M13F</td><td>AK1R</td><td>147 7</td></tr><tr><td>36</td><td>M13F</td><td>AK1R</td><td>141 1-1</td></tr><tr><td>37</td><td>M13F</td><td>AK1R</td><td>141 1-2</td></tr><tr><td>38</td><td>M13F</td><td>AK1R</td><td>141 1-4</td></tr><tr><td>39</td><td>M13F</td><td>AK1R</td><td>148 2-3</td></tr><tr><td>40</td><td>M13F</td><td>AK1R</td><td>148 2-5</td></tr><tr><td>41</td><td>M13F</td><td>AK1R</td><td>148 2-4</td></tr><tr><td>42</td><td>M13F</td><td>AK1R</td><td>148 1-5</td></tr><tr><td>43</td><td>M13F</td><td>AK1R</td><td>148 1-3</td></tr><tr><td>44</td><td>M13F</td><td>AK1R</td><td>144 11</td></tr><tr><td>45</td><td>M13F</td><td>AK1R</td><td>144 3</td></tr><tr><td>46</td><td>M13F</td><td>AK1R</td><td>144 1</td></tr><tr><td>47</td><td>M13F</td><td>AK1R</td><td>146 1-6</td></tr><tr><td>48</td><td>M13F</td><td>AK1R</td><td>146 1-4</td></tr><tr><td>49</td><td>M13F</td><td>AK1R</td><td>146 2-5</td></tr><tr><td>50</td><td>M13F</td><td>AK1R</td><td>146 2-7</td></tr><tr><td>51</td><td>M13F</td><td>AK1R</td><td>142 2-2</td></tr><tr><td>52</td><td>M13F</td><td>AK1R</td><td>142 1-1</td></tr><tr><td>53</td><td>M13F</td><td>AK1R</td><td>142 2-7</td></tr><tr><td>54</td><td>M13F</td><td>AK1R</td><td>142 2-3</td></tr><tr><td>55</td><td>M13F</td><td>AK1R</td><td>145 2-11</td></tr><tr><td>56</td><td>M13F</td><td>AK1R</td><td>145 1-17</td></tr><tr><td>57</td><td>M13F</td><td>AK1R</td><td>145 2-9</td></tr><tr><td>58</td><td>M13F</td><td>AK1R</td><td>145 2-17</td></tr><tr><td>59</td><td>M13F</td><td>AK1R</td><td>143 5</td></tr><tr><td>60</td><td>M13F</td><td>AK1R</td><td>143 17</td></tr><tr><td>61</td><td>M13F</td><td>AK1R</td><td>143 15</td></tr><tr><td>62</td><td>M13F</td><td>AK1R</td><td>143 21</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi robust <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/e/e9/IGKU_b2_068_0.jpeg"><img src="https://static.igem.org/mediawiki/2017/7/7d/IGKU_b2_068_1.jpeg"><img src="https://static.igem.org/mediawiki/2017/5/50/IGKU_b2_068_2.jpeg"><img src="https://static.igem.org/mediawiki/2017/f/f2/IGKU_b2_068_3.jpeg">
| |
| − | <!-- IGKU_b2_068_4.jpeg -->
| |
| − | <!-- IGKU_b2_068_5.jpeg -->
| |
| − | <!-- IGKU_b2_068_6.jpeg -->
| |
| − | <!-- IGKU_b2_068_7.jpeg -->
| |
| − | <!-- IGKU_b2_068_8.jpeg -->
| |
| − | <!-- IGKU_b2_068_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0824" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/24</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>141 2-1</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>141 2-2</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>141 2-4</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>141 2-6</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>142 1-5</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>142 2-4</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>142 1-3</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>142 1-4</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>143 13</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>143 19</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>143 25</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>144 18</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>144 17</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>144 5</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>144 9</td></tr><tr><td>16</td><td>M13F</td><td>M13R</td><td>143 19</td></tr><tr><td>17</td><td>M13F</td><td>M13R</td><td>145 1-5</td></tr><tr><td>18</td><td>M13F</td><td>M13R</td><td>145 2-15</td></tr><tr><td>19</td><td>M13F</td><td>M13R</td><td>143 2-13</td></tr><tr><td>20</td><td>M13F</td><td>M13R</td><td>145 1-13</td></tr><tr><td>21</td><td>M13F</td><td>AK1R</td><td>141 2-1</td></tr><tr><td>22</td><td>M13F</td><td>AK1R</td><td>141 2-2</td></tr><tr><td>23</td><td>M13F</td><td>AK1R</td><td>141 2-4</td></tr><tr><td>24</td><td>M13F</td><td>AK1R</td><td>141 2-6</td></tr><tr><td>25</td><td>M13F</td><td>AK1R</td><td>142 1-5</td></tr><tr><td>26</td><td>M13F</td><td>AK1R</td><td>142 2-4</td></tr><tr><td>27</td><td>M13F</td><td>AK1R</td><td>142 1-3</td></tr><tr><td>28</td><td>M13F</td><td>AK1R</td><td>142 1-4</td></tr><tr><td>29</td><td>M13F</td><td>AK1R</td><td>145 1-5</td></tr><tr><td>30</td><td>M13F</td><td>AK1R</td><td>145 2-15</td></tr><tr><td>31</td><td>M13F</td><td>AK1R</td><td>143 2-13</td></tr><tr><td>32</td><td>M13F</td><td>AK1R</td><td>145 1-13</td></tr><tr><td>33</td><td>M13F</td><td>AK1R</td><td>144 17</td></tr><tr><td>34</td><td>M13F</td><td>AK1R</td><td>144 5</td></tr><tr><td>35</td><td>M13F</td><td>AK1R</td><td>144 9</td></tr><tr><td>36</td><td>M13F</td><td>AK1R</td><td>143 19</td></tr><tr><td>37</td><td>M13F</td><td>AK1R</td><td>143 13</td></tr><tr><td>38</td><td>M13F</td><td>AK1R</td><td>143 19</td></tr><tr><td>39</td><td>M13F</td><td>AK1R</td><td>143 25</td></tr><tr><td>40</td><td>M13F</td><td>AK1R</td><td>144 9</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA Robust <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/4/4c/IGKU_b2_070_0.jpeg">
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| − | <!-- IGKU_b2_070_1.jpeg -->
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| − | <!-- IGKU_b2_070_2.jpeg -->
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| − | <!-- IGKU_b2_070_3.jpeg -->
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| − | <!-- IGKU_b2_070_4.jpeg -->
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| − | <!-- IGKU_b2_070_5.jpeg -->
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| − | <!-- IGKU_b2_070_6.jpeg -->
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| − | <!-- IGKU_b2_070_7.jpeg -->
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| − | <!-- IGKU_b2_070_8.jpeg -->
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| − | <!-- IGKU_b2_070_9.jpeg -->
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| − | </div>
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| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0826" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/26</h3></span>
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| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK27</td><td>IK30</td><td>GPDp + Rev after gel extraction</td></tr><tr><td>2</td><td>IK27</td><td>IK30</td><td>GPDp + Rev after gel extraction</td></tr><tr><td>3</td><td>IK27</td><td>IK30</td><td>GPDp + Rev after gel extraction</td></tr><tr><td>4</td><td>IK27</td><td>IK30</td><td>GPDp + Rev after gel extraction</td></tr><tr><td>5</td><td>IK23</td><td>IK28</td><td>GPDt after PCR purification</td></tr><tr><td>6</td><td>IK23</td><td>IK28</td><td>GPDt after PCR purification</td></tr><tr><td>7</td><td>IK23</td><td>IK28</td><td>GPDt after PCR purification</td></tr><tr><td>8</td><td>IK23</td><td>IK28</td><td>GPDt after PCR purification</td></tr>
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| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi HS Readymix <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/4/40/IGKU_b2_072_0.jpeg">
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| − | <!-- IGKU_b2_072_1.jpeg -->
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| − | <!-- IGKU_b2_072_2.jpeg -->
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| − | <!-- IGKU_b2_072_3.jpeg -->
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| − | <!-- IGKU_b2_072_4.jpeg -->
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| − | <!-- IGKU_b2_072_5.jpeg -->
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| − | <!-- IGKU_b2_072_6.jpeg -->
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| − | <!-- IGKU_b2_072_7.jpeg -->
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| − | <!-- IGKU_b2_072_8.jpeg -->
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| − | <!-- IGKU_b2_072_9.jpeg -->
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| − | </div>
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| − | <!------------ Table END ------------>
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| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0826" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/26</h3></span>
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| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>145-2 11</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>145-1 9</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>145 2-7</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>145 1-21</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>145 1-3</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>145 2-3</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>145 1-19</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>142 1-2</td></tr><tr><td>9</td><td>M13F</td><td>AK1F</td><td>145-2 11</td></tr><tr><td>10</td><td>M13F</td><td>AK1F</td><td>145-1 9</td></tr><tr><td>11</td><td>M13F</td><td>AK1F</td><td>145 2-7</td></tr><tr><td>12</td><td>M13F</td><td>AK1F</td><td>145 1-21</td></tr><tr><td>13</td><td>M13F</td><td>AK1F</td><td>145 1-3</td></tr><tr><td>14</td><td>M13F</td><td>AK1F</td><td>145 2-3</td></tr><tr><td>15</td><td>M13F</td><td>AK1F</td><td>145 1-19</td></tr><tr><td>16</td><td>M13F</td><td>AK1F</td><td>142 1-2</td></tr>
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| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA hifi <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 25sec<br>72.0°C : 3min<br>4.0°C : Infinate<br>X35 cycle</p>
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| − | <!-- IGKU_b2_073_0.jpeg -->
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| − | <!-- IGKU_b2_073_1.jpeg -->
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| − | <!-- IGKU_b2_073_2.jpeg -->
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| − | <!-- IGKU_b2_073_3.jpeg -->
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| − | <!-- IGKU_b2_073_4.jpeg -->
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| − | <!-- IGKU_b2_073_5.jpeg -->
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| − | <!-- IGKU_b2_073_6.jpeg -->
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| − | <!-- IGKU_b2_073_7.jpeg -->
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| − | <!-- IGKU_b2_073_8.jpeg -->
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| − | <!-- IGKU_b2_073_9.jpeg -->
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| − | </div>
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| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0826" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/26</h3></span>
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| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>114-1</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>114-2</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>172</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>172-1</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>172-2</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>172-4</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>175-3</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>175-10</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>176</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>177</td><td>2.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_074_0.jpeg -->
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| − | <!-- IGKU_b2_074_1.jpeg -->
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| − | <!-- IGKU_b2_074_2.jpeg -->
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| − | <!-- IGKU_b2_074_3.jpeg -->
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| − | <!-- IGKU_b2_074_4.jpeg -->
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| − | <!-- IGKU_b2_074_5.jpeg -->
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| − | <!-- IGKU_b2_074_6.jpeg -->
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| − | <!-- IGKU_b2_074_7.jpeg -->
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| − | <!-- IGKU_b2_074_8.jpeg -->
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| − | <!-- IGKU_b2_074_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0827" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/27</h3></span>
| |
| − | <span class="keln_exp"><h4>Nebuilder</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>Sample</th><th>Vectorname</th><th>Vector amount (ul)</th><th> Insert1 name</th><th> Insert1 amount(ul)</th><th>Insert2 name </th><th>Insert2 amount (ul)</th><th>Mastermix amount (ul)</th><th>Total amount(ul)</th></tr><tr><td>1</td><td>Rev</td><td>3.02</td><td>GPDp+Rev</td><td>0.669</td><td>GPDt</td><td>0.875</td><td>10.0</td><td>Total amount(ul)</td></tr><tr><td>2</td><td>173.0</td><td>3.02</td><td>164.0</td><td>0.325</td><td>165.0</td><td>0.285</td><td>5.0</td><td>Total amount(ul)</td></tr><tr><td>3</td><td>175.0</td><td>3.02</td><td>161.0</td><td>0.19</td><td>167.0</td><td>0.42</td><td>5.0</td><td>Total amount(ul)</td></tr><tr><td>4</td><td>178.0</td><td>3.02</td><td>163.0</td><td>0.41</td><td>167.0</td><td>0.42</td><td>5.0</td><td>Total amount(ul)</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_075_0.jpeg -->
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| − | <!-- IGKU_b2_075_1.jpeg -->
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| − | <!-- IGKU_b2_075_2.jpeg -->
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| − | <!-- IGKU_b2_075_3.jpeg -->
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| − | <!-- IGKU_b2_075_4.jpeg -->
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| − | <!-- IGKU_b2_075_5.jpeg -->
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| − | <!-- IGKU_b2_075_6.jpeg -->
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| − | <!-- IGKU_b2_075_7.jpeg -->
| |
| − | <!-- IGKU_b2_075_8.jpeg -->
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| − | <!-- IGKU_b2_075_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0827" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/27</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>171-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>171-1-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>172-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>172-2-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>172-3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>172-4</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>172-5</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>172-6</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>172-8</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>172-9</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>11</td><td>172-10</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>12</td><td>172-11</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>13</td><td>172-12</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>14</td><td>172-13</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>15</td><td>175-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>16</td><td>175-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>17</td><td>175-3</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>18</td><td>175-4</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>19</td><td>176-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>20</td><td>177-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>21</td><td>177-3</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>22</td><td>177-4</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>23</td><td>177-5</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>24</td><td>177-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>25</td><td>177-1-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>26</td><td>171-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>27</td><td>172-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>28</td><td>175-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>29</td><td>175-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>30</td><td>175-3</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>31</td><td>175-4</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>32</td><td>176-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>33</td><td>176-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>34</td><td>177-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>35</td><td>177-3</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>36</td><td>177-4</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>37</td><td>177-5</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
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| − | </table>
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| − | <!-- IGKU_b2_076_8.jpeg -->
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| − | <!-- IGKU_b2_076_9.jpeg -->
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| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0827" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/27</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>171 1-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>171 1-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>171 2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>171 3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>172-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>172-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>172-3</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>172-4-1</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>172-4-2</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>172-5</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>11</td><td>172-6</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>12</td><td>172-8</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>13</td><td>172-9</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>14</td><td>172-10</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>15</td><td>172-11</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>16</td><td>172-12</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>17</td><td>172-13</td><td>5.0</td><td>Pst1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>18</td><td>171-1-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>19</td><td>174-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>20</td><td>175-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>21</td><td>175-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>22</td><td>175-3</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>23</td><td>175-4</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>24</td><td>176-1</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>25</td><td>176-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>26</td><td>177-2</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>27</td><td>177-3</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>28</td><td>177-4</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr><tr><td>29</td><td>177-5</td><td>5.0</td><td>EcoR1</td><td>1.0</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
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| − | </table>
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| − | <!-- IGKU_b2_077_0.jpeg -->
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| − | <!-- IGKU_b2_077_1.jpeg -->
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| − | <!-- IGKU_b2_077_2.jpeg -->
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| − | <!-- IGKU_b2_077_3.jpeg -->
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| − | <!-- IGKU_b2_077_9.jpeg -->
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| − | </div>
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| − | <!------------ Table END ------------>
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| − | <!-- Table Generated by KELN Author: ohara -->
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| − | <div class="keln_container">
| |
| − | <a name="0828" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/28</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>145-2</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>142 2-11</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>142 2-2</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>142 2-1</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>142 2-3</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>242 1-4</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>7</td><td>142 2-4</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>8</td><td>142 1-5</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>9</td><td>142 1-3</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>10</td><td>142 2-5</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>11</td><td>142 1-2</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>12</td><td>142 1-1</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>13</td><td>145 2-3</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>14</td><td>145 1-2</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>15</td><td>145 2-7</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>16</td><td>145 4-9</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>17</td><td>145 1-11</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>18</td><td>145 1-3</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>19</td><td>145 2-11</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>20</td><td>145 2-5</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>21</td><td>145 2-8</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>22</td><td>145 1-14</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>23</td><td>145 2-15</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>24</td><td>145 2- 4-2</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>25</td><td>145 1-17</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>26</td><td>145 2-17</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr>
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| − | </table>
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| − | <!-- IGKU_b2_078_0.jpeg -->
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| − | <!-- IGKU_b2_078_1.jpeg -->
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| − | </div>
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| − | <!------------ Table END ------------>
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| − | <!-- Table Generated by KELN Author: ohara -->
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| − | <div class="keln_container">
| |
| − | <a name="0829" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/29</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>142 1-3</td><td>5.0</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul 1ul</td><td>10.0</td></tr><tr><td>2</td><td>145 1-11</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul 1ul</td><td>9.0</td></tr><tr><td>3</td><td>145 1-3</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul 1ul</td><td>9.0</td></tr><tr><td>4</td><td>145 2-42</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul 1ul</td><td>9.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_079_0.jpeg -->
| |
| − | <!-- IGKU_b2_079_1.jpeg -->
| |
| − | <!-- IGKU_b2_079_2.jpeg -->
| |
| − | <!-- IGKU_b2_079_3.jpeg -->
| |
| − | <!-- IGKU_b2_079_4.jpeg -->
| |
| − | <!-- IGKU_b2_079_5.jpeg -->
| |
| − | <!-- IGKU_b2_079_6.jpeg -->
| |
| − | <!-- IGKU_b2_079_7.jpeg -->
| |
| − | <!-- IGKU_b2_079_8.jpeg -->
| |
| − | <!-- IGKU_b2_079_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0829" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/29</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK27</td><td>IK28</td><td>Rev, GPDp, GPDt</td></tr><tr><td>2</td><td>IK27</td><td>IK28</td><td>Rev+GPDp, GPDt</td></tr><tr><td>3</td><td>IK27</td><td>IK28</td><td>Rev plasmid</td></tr><tr><td>4</td><td>IK27</td><td>IK28</td><td>Rev plasmid</td></tr><tr><td>5</td><td>IK27</td><td>IK28</td><td>Rev plasmid</td></tr><tr><td>6</td><td>IK27</td><td>IK28</td><td>Rev plasmid</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAKA Robust <br>95.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 15sec<br>72.0°C : 1min30sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_080_0.jpeg -->
| |
| − | <!-- IGKU_b2_080_1.jpeg -->
| |
| − | <!-- IGKU_b2_080_2.jpeg -->
| |
| − | <!-- IGKU_b2_080_3.jpeg -->
| |
| − | <!-- IGKU_b2_080_4.jpeg -->
| |
| − | <!-- IGKU_b2_080_5.jpeg -->
| |
| − | <!-- IGKU_b2_080_6.jpeg -->
| |
| − | <!-- IGKU_b2_080_7.jpeg -->
| |
| − | <!-- IGKU_b2_080_8.jpeg -->
| |
| − | <!-- IGKU_b2_080_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0831" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>08/31</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>141 1-2</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Xba1</td><td>0.5</td><td>10xN</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>141 1-2</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Xba1</td><td>0.5</td><td>10xN</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>141 1-3</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Xba1</td><td>0.5</td><td>10xN</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>141 1-3</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Xba1</td><td>0.5</td><td>10xN</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>144-9</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>6</td><td>144-9</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>7</td><td>144 17</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>8</td><td>144 17</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>9</td><td>144 3</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>10</td><td>144 3</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>11</td><td>144 3</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>12</td><td>144 1</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>13</td><td>144 1</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>14</td><td>144 1</td><td>3.5</td><td>EcoR1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xH, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>15</td><td>143 21</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>16</td><td>143 21</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>17</td><td>143 21</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>18</td><td>143 15</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>19</td><td>143 15</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>20</td><td>143 15</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>21</td><td>143 15</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>22</td><td>143 5</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>23</td><td>143 5</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>24</td><td>143 5</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>25</td><td>143 13</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>26</td><td>145 1-3</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>27</td><td>145 1-3</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>28</td><td>145 1-3</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>Not1</td><td>0.5</td><td>10xK, BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>29</td><td>142 2-2</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>30</td><td>142 1-3</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>31</td><td>142</td><td>5.0</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>32</td><td>142 2-2</td><td>5.0</td><td>Not1</td><td>0.5</td><td>Xba1</td><td>0.5</td><td>10xK BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>33</td><td>142 1-3</td><td>5.0</td><td>Not1</td><td>0.5</td><td>Xba1</td><td>0.5</td><td>10xK BSA</td><td>1ul, 1ul</td><td>10.0</td></tr><tr><td>34</td><td>142</td><td>5.0</td><td>Not1</td><td>0.5</td><td>Xba1</td><td>0.5</td><td>10xK BSA</td><td>1ul, 1ul</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_082_0.jpeg -->
| |
| − | <!-- IGKU_b2_082_1.jpeg -->
| |
| − | <!-- IGKU_b2_082_2.jpeg -->
| |
| − | <!-- IGKU_b2_082_3.jpeg -->
| |
| − | <!-- IGKU_b2_082_4.jpeg -->
| |
| − | <!-- IGKU_b2_082_5.jpeg -->
| |
| − | <!-- IGKU_b2_082_6.jpeg -->
| |
| − | <!-- IGKU_b2_082_7.jpeg -->
| |
| − | <!-- IGKU_b2_082_8.jpeg -->
| |
| − | <!-- IGKU_b2_082_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0901" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/01</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>P1</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>P2</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>P3</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>P4</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>T1</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>T2</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>T3</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>T4</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>98.0°C : 3min<br>98.0°C : 30sec<br>60.0°C : 20sec<br>72.0°C : 40sec<br>72.0°C : 10min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/f/ff/IGKU_b2_083_0.jpeg">
| |
| − | <!-- IGKU_b2_083_1.jpeg -->
| |
| − | <!-- IGKU_b2_083_2.jpeg -->
| |
| − | <!-- IGKU_b2_083_3.jpeg -->
| |
| − | <!-- IGKU_b2_083_4.jpeg -->
| |
| − | <!-- IGKU_b2_083_5.jpeg -->
| |
| − | <!-- IGKU_b2_083_6.jpeg -->
| |
| − | <!-- IGKU_b2_083_7.jpeg -->
| |
| − | <!-- IGKU_b2_083_8.jpeg -->
| |
| − | <!-- IGKU_b2_083_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0902" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/02</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>144-3</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>142</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>145 1-11</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/d/df/IGKU_b2_085_0.jpeg" class="1"><img src="https://static.igem.org/mediawiki/2017/8/84/IGKU_b2_085_1.jpeg" class="1">
| |
| − | <!-- IGKU_b2_085_2.jpeg -->
| |
| − | <!-- IGKU_b2_085_3.jpeg -->
| |
| − | <!-- IGKU_b2_085_4.jpeg -->
| |
| − | <!-- IGKU_b2_085_5.jpeg -->
| |
| − | <!-- IGKU_b2_085_6.jpeg -->
| |
| − | <!-- IGKU_b2_085_7.jpeg -->
| |
| − | <!-- IGKU_b2_085_8.jpeg -->
| |
| − | <!-- IGKU_b2_085_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0902" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/02</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>165-1</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>165-2</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>165-3</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>165-4</td></tr><tr><td>5</td><td>M13F</td><td>IK60</td><td>165-1</td></tr><tr><td>6</td><td>M13F</td><td>IK60</td><td>165-2</td></tr><tr><td>7</td><td>M13F</td><td>IK60</td><td>165-3</td></tr><tr><td>8</td><td>M13F</td><td>IK60</td><td>165-4</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 30sec<br>60.0°C : 20sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/1/18/IGKU_b2_086_0.jpeg">
| |
| − | <!-- IGKU_b2_086_1.jpeg -->
| |
| − | <!-- IGKU_b2_086_2.jpeg -->
| |
| − | <!-- IGKU_b2_086_3.jpeg -->
| |
| − | <!-- IGKU_b2_086_4.jpeg -->
| |
| − | <!-- IGKU_b2_086_5.jpeg -->
| |
| − | <!-- IGKU_b2_086_6.jpeg -->
| |
| − | <!-- IGKU_b2_086_7.jpeg -->
| |
| − | <!-- IGKU_b2_086_8.jpeg -->
| |
| − | <!-- IGKU_b2_086_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0902" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/02</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>142 2-2</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>1421</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>142 1-3</td><td>3.5</td><td>Xba1</td><td>0.5</td><td>EcoR1</td><td>0.5</td><td>10xM</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_087_0.jpeg -->
| |
| − | <!-- IGKU_b2_087_1.jpeg -->
| |
| − | <!-- IGKU_b2_087_2.jpeg -->
| |
| − | <!-- IGKU_b2_087_3.jpeg -->
| |
| − | <!-- IGKU_b2_087_4.jpeg -->
| |
| − | <!-- IGKU_b2_087_5.jpeg -->
| |
| − | <!-- IGKU_b2_087_6.jpeg -->
| |
| − | <!-- IGKU_b2_087_7.jpeg -->
| |
| − | <!-- IGKU_b2_087_8.jpeg -->
| |
| − | <!-- IGKU_b2_087_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0903" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/03</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>0.35</td><td>161</td><td>6.19</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>0.35</td><td>162</td><td>12.2</td></tr><tr><td>3</td><td>YEplac195/sma1</td><td>0.35</td><td>163</td><td>13.1</td></tr><tr><td>4</td><td>YEplac195/sma1</td><td>0.35</td><td>164</td><td>10.32</td></tr><tr><td>5</td><td>YEplac195/sma1</td><td>0.35</td><td>166</td><td>17.25</td></tr><tr><td>6</td><td>YEplac195/sma1</td><td>0.35</td><td>167</td><td>13.28</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_087_0.jpeg -->
| |
| − | <!-- IGKU_b2_087_1.jpeg -->
| |
| − | <!-- IGKU_b2_087_2.jpeg -->
| |
| − | <!-- IGKU_b2_087_3.jpeg -->
| |
| − | <!-- IGKU_b2_087_4.jpeg -->
| |
| − | <!-- IGKU_b2_087_5.jpeg -->
| |
| − | <!-- IGKU_b2_087_6.jpeg -->
| |
| − | <!-- IGKU_b2_087_7.jpeg -->
| |
| − | <!-- IGKU_b2_087_8.jpeg -->
| |
| − | <!-- IGKU_b2_087_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0903" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/03</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>IK55</td><td>GFPgalp-1</td></tr><tr><td>2</td><td>M13F</td><td>IK55</td><td>GFPgalp-2</td></tr><tr><td>3</td><td>M13F</td><td>IK55</td><td>GFPgalp-3</td></tr><tr><td>4</td><td>M13F</td><td>IK55</td><td>GFPgalp-4</td></tr><tr><td>5</td><td>M13F</td><td>IK55</td><td>GFPt-1</td></tr><tr><td>6</td><td>M13F</td><td>IK55</td><td>GFPt-2</td></tr><tr><td>7</td><td>M13F</td><td>IK55</td><td>GFPt-3</td></tr><tr><td>8</td><td>M13F</td><td>IK55</td><td>GFPt-4</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 30sec<br>60.0°C : 20sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_088_0.jpeg -->
| |
| − | <!-- IGKU_b2_088_1.jpeg -->
| |
| − | <!-- IGKU_b2_088_2.jpeg -->
| |
| − | <!-- IGKU_b2_088_3.jpeg -->
| |
| − | <!-- IGKU_b2_088_4.jpeg -->
| |
| − | <!-- IGKU_b2_088_5.jpeg -->
| |
| − | <!-- IGKU_b2_088_6.jpeg -->
| |
| − | <!-- IGKU_b2_088_7.jpeg -->
| |
| − | <!-- IGKU_b2_088_8.jpeg -->
| |
| − | <!-- IGKU_b2_088_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0904" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/04</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>4</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>5</td><td>IK23</td><td>IK28</td><td>GPDter</td></tr><tr><td>6</td><td>IK23</td><td>IK28</td><td>GPDter</td></tr><tr><td>7</td><td>IK23</td><td>IK28</td><td>GPDter</td></tr><tr><td>8</td><td>IK23</td><td>IK28</td><td>GPDter</td></tr><tr><td>9</td><td>IK29</td><td>IK30</td><td>Rev long</td></tr><tr><td>10</td><td>IK29</td><td>IK30</td><td>Rev long</td></tr><tr><td>11</td><td>IK29</td><td>IK30</td><td>Rev long</td></tr><tr><td>12</td><td>IK29</td><td>IK30</td><td>Rev long</td></tr><tr><td>13</td><td>IK21</td><td>IK28</td><td>GPDter(PCRp), Rev(PCRp), GPDp(gel ex)</td></tr><tr><td>14</td><td>IK21</td><td>IK28</td><td>GPDter(PCRp), Rev+GPDp(PCRp)</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min20sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: 1~4<br>PCR pulification: 9~12<br>Gel extraction: 1~4<br>Gel extraction: 9~12</p><img src="https://static.igem.org/mediawiki/2017/8/8e/IGKU_b2_090_0.jpeg">
| |
| − | <!-- IGKU_b2_090_1.jpeg -->
| |
| − | <!-- IGKU_b2_090_2.jpeg -->
| |
| − | <!-- IGKU_b2_090_3.jpeg -->
| |
| − | <!-- IGKU_b2_090_4.jpeg -->
| |
| − | <!-- IGKU_b2_090_5.jpeg -->
| |
| − | <!-- IGKU_b2_090_6.jpeg -->
| |
| − | <!-- IGKU_b2_090_7.jpeg -->
| |
| − | <!-- IGKU_b2_090_8.jpeg -->
| |
| − | <!-- IGKU_b2_090_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0904" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/04</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK60</td><td>AK1F</td><td>142-2-1</td></tr><tr><td>2</td><td>IK60</td><td>AK1F</td><td>142-2-2</td></tr><tr><td>3</td><td>IK60</td><td>AK1F</td><td>142-2-3</td></tr><tr><td>4</td><td>IK60</td><td>AK1F</td><td>142-2-4</td></tr><tr><td>5</td><td>IK60</td><td>AK1F</td><td>142-2-5</td></tr><tr><td>6</td><td>IK60</td><td>AK1F</td><td>142-1-1</td></tr><tr><td>7</td><td>IK60</td><td>AK1F</td><td>142-1-2</td></tr><tr><td>8</td><td>IK60</td><td>AK1F</td><td>142-1-3</td></tr><tr><td>9</td><td>IK60</td><td>AK1F</td><td>142-1-4</td></tr><tr><td>10</td><td>IK60</td><td>AK1F</td><td>142-1-5</td></tr><tr><td>11</td><td>IK60</td><td>M13F</td><td>144-3</td></tr><tr><td>12</td><td>IK60</td><td>M13R</td><td>145-1-11</td></tr><tr><td>13</td><td>IK60</td><td>AK1F</td><td>144-3</td></tr><tr><td>14</td><td>IK60</td><td>AK1F</td><td>145-1-11</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 30sec<br>60.0°C : 20sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/3/32/IGKU_b2_091_0.jpeg">
| |
| − | <!-- IGKU_b2_091_1.jpeg -->
| |
| − | <!-- IGKU_b2_091_2.jpeg -->
| |
| − | <!-- IGKU_b2_091_3.jpeg -->
| |
| − | <!-- IGKU_b2_091_4.jpeg -->
| |
| − | <!-- IGKU_b2_091_5.jpeg -->
| |
| − | <!-- IGKU_b2_091_6.jpeg -->
| |
| − | <!-- IGKU_b2_091_7.jpeg -->
| |
| − | <!-- IGKU_b2_091_8.jpeg -->
| |
| − | <!-- IGKU_b2_091_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0904" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/04</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK60</td><td>M13R</td><td>142-2-1</td></tr><tr><td>2</td><td>IK60</td><td>M13R</td><td>142-2-2</td></tr><tr><td>3</td><td>IK60</td><td>M13R</td><td>142-2-3</td></tr><tr><td>4</td><td>IK60</td><td>M13R</td><td>142-2-4</td></tr><tr><td>5</td><td>IK60</td><td>M13R</td><td>142-2-5</td></tr><tr><td>6</td><td>IK60</td><td>M13R</td><td>142-1-1</td></tr><tr><td>7</td><td>IK60</td><td>M13R</td><td>142-1-2</td></tr><tr><td>8</td><td>IK60</td><td>M13R</td><td>142-1-3</td></tr><tr><td>9</td><td>IK60</td><td>M13R</td><td>142-1-4</td></tr><tr><td>10</td><td>IK60</td><td>M13R</td><td>142-1-5</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>gfpp</td></tr><tr><td>12</td><td>IK55</td><td>M13R</td><td>gfpp</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>gfpt M13</td></tr><tr><td>14</td><td>IK55</td><td>M13R</td><td>gfp 55</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 20sec<br>61.0°C : 20sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/5/5b/IGKU_b2_092_0.jpeg">
| |
| − | <!-- IGKU_b2_092_1.jpeg -->
| |
| − | <!-- IGKU_b2_092_2.jpeg -->
| |
| − | <!-- IGKU_b2_092_3.jpeg -->
| |
| − | <!-- IGKU_b2_092_4.jpeg -->
| |
| − | <!-- IGKU_b2_092_5.jpeg -->
| |
| − | <!-- IGKU_b2_092_6.jpeg -->
| |
| − | <!-- IGKU_b2_092_7.jpeg -->
| |
| − | <!-- IGKU_b2_092_8.jpeg -->
| |
| − | <!-- IGKU_b2_092_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0904" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/04</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK27</td><td>IK30</td><td>Rev+p</td></tr><tr><td>2</td><td>IK21</td><td>IK30</td><td>Rev+p</td></tr><tr><td>3</td><td>IK27</td><td>IK22</td><td>GPDp</td></tr><tr><td>4</td><td>IK27</td><td>IK22</td><td>GPDp</td></tr><tr><td>5</td><td>IK27</td><td>IK22</td><td>GPDp</td></tr><tr><td>6</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>7</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr><tr><td>8</td><td>IK21</td><td>IK22</td><td>GPDp</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 15sec<br>60.0°C : 15sec<br>72.0°C : 30sec<br>72.0°C : 2min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_096_0.jpeg -->
| |
| − | <!-- IGKU_b2_096_1.jpeg -->
| |
| − | <!-- IGKU_b2_096_2.jpeg -->
| |
| − | <!-- IGKU_b2_096_3.jpeg -->
| |
| − | <!-- IGKU_b2_096_4.jpeg -->
| |
| − | <!-- IGKU_b2_096_5.jpeg -->
| |
| − | <!-- IGKU_b2_096_6.jpeg -->
| |
| − | <!-- IGKU_b2_096_7.jpeg -->
| |
| − | <!-- IGKU_b2_096_8.jpeg -->
| |
| − | <!-- IGKU_b2_096_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0904" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/04</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Fukuda</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>eef-1g F</td><td>eef-1gR</td><td>3</td></tr><tr><td>2</td><td>eef-1g F</td><td>eef-1gR</td><td>4</td></tr><tr><td>3</td><td>AK1F</td><td>AK1R</td><td>5</td></tr><tr><td>4</td><td>AK1F</td><td>AK1R</td><td>6</td></tr><tr><td>5</td><td>asbF</td><td>asbR</td><td>7</td></tr><tr><td>6</td><td>asbF</td><td>asbR</td><td>8</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA Fast <br>95.0°C : 3min<br>95.0°C : 15sec<br>60.0°C : 15sec<br>72.0°C : 12sec<br>72.0°C : 48sec<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/6/64/IGKU_b2_106_0.jpeg"><img src="https://static.igem.org/mediawiki/2017/8/8b/IGKU_b2_106_1.jpeg">
| |
| − | <!-- IGKU_b2_106_2.jpeg -->
| |
| − | <!-- IGKU_b2_106_3.jpeg -->
| |
| − | <!-- IGKU_b2_106_4.jpeg -->
| |
| − | <!-- IGKU_b2_106_5.jpeg -->
| |
| − | <!-- IGKU_b2_106_6.jpeg -->
| |
| − | <!-- IGKU_b2_106_7.jpeg -->
| |
| − | <!-- IGKU_b2_106_8.jpeg -->
| |
| − | <!-- IGKU_b2_106_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0905" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/05</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>161</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>161</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>162</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>163</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>166</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>167</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>168</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>142</td></tr><tr><td>9</td><td>IK60</td><td>M13R</td><td>161</td></tr><tr><td>10</td><td>IK60</td><td>M13R</td><td>161</td></tr><tr><td>11</td><td>M13F</td><td>IK60</td><td>162</td></tr><tr><td>12</td><td>IK60</td><td>M13R</td><td>163</td></tr><tr><td>13</td><td>IK60</td><td>M13R</td><td>166</td></tr><tr><td>14</td><td>M13F</td><td>IK60</td><td>167</td></tr><tr><td>15</td><td>M13F</td><td>IK60</td><td>168</td></tr><tr><td>16</td><td>M13F</td><td>IK60</td><td>142</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 20sec<br>61.0°C : 20sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/9/95/IGKU_b2_093_0.jpeg">
| |
| − | <!-- IGKU_b2_093_1.jpeg -->
| |
| − | <!-- IGKU_b2_093_2.jpeg -->
| |
| − | <!-- IGKU_b2_093_3.jpeg -->
| |
| − | <!-- IGKU_b2_093_4.jpeg -->
| |
| − | <!-- IGKU_b2_093_5.jpeg -->
| |
| − | <!-- IGKU_b2_093_6.jpeg -->
| |
| − | <!-- IGKU_b2_093_7.jpeg -->
| |
| − | <!-- IGKU_b2_093_8.jpeg -->
| |
| − | <!-- IGKU_b2_093_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0905" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/05</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>gfpp</td><td>5.0</td><td>EcoRI</td><td>2.0</td><td>None</td><td>None</td><td>H</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>gfpp</td><td>5.0</td><td>Not1</td><td>1.0</td><td>HindⅢ</td><td>1.0</td><td>K</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/c/c8/IGKU_b2_094_0.jpeg">
| |
| − | <!-- IGKU_b2_094_1.jpeg -->
| |
| − | <!-- IGKU_b2_094_2.jpeg -->
| |
| − | <!-- IGKU_b2_094_3.jpeg -->
| |
| − | <!-- IGKU_b2_094_4.jpeg -->
| |
| − | <!-- IGKU_b2_094_5.jpeg -->
| |
| − | <!-- IGKU_b2_094_6.jpeg -->
| |
| − | <!-- IGKU_b2_094_7.jpeg -->
| |
| − | <!-- IGKU_b2_094_8.jpeg -->
| |
| − | <!-- IGKU_b2_094_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0905" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/05</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK28</td><td>Rev, p, t</td></tr><tr><td>2</td><td>IK21</td><td>IK28</td><td>Rev+p, t</td></tr><tr><td>3</td><td>IK27</td><td>IK28</td><td>Rev, p, t</td></tr><tr><td>4</td><td>IK27</td><td>IK28</td><td>Rev+p, t</td></tr><tr><td>5</td><td>IK21</td><td>IK24</td><td>Rev, p, t</td></tr><tr><td>6</td><td>IK21</td><td>IK24</td><td>Rev+p, t</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 15sec<br>55.0°C : 15sec<br>72.0°C : 30sec<br>72.0°C : 2min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/b/b2/IGKU_b2_095_0.jpeg">
| |
| − | <!-- IGKU_b2_095_1.jpeg -->
| |
| − | <!-- IGKU_b2_095_2.jpeg -->
| |
| − | <!-- IGKU_b2_095_3.jpeg -->
| |
| − | <!-- IGKU_b2_095_4.jpeg -->
| |
| − | <!-- IGKU_b2_095_5.jpeg -->
| |
| − | <!-- IGKU_b2_095_6.jpeg -->
| |
| − | <!-- IGKU_b2_095_7.jpeg -->
| |
| − | <!-- IGKU_b2_095_8.jpeg -->
| |
| − | <!-- IGKU_b2_095_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0905" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/05</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK30</td><td>9/5 PCR p Rev,pro</td></tr><tr><td>2</td><td>IK21</td><td>IK30</td><td>9/5 PCR p Rev,pro</td></tr><tr><td>3</td><td>IK21</td><td>IK30</td><td>9/5 PCR p Rev,pro</td></tr><tr><td>4</td><td>IK21</td><td>IK30</td><td>9/5 PCR p Rev,pro</td></tr><tr><td>5</td><td>IK29</td><td>IK24</td><td>9/5 PCR p Rev,ter</td></tr><tr><td>6</td><td>IK29</td><td>IK24</td><td>9/5 PCR p Rev,ter</td></tr><tr><td>7</td><td>IK29</td><td>IK24</td><td>9/5 PCR p Rev,ter</td></tr><tr><td>8</td><td>IK29</td><td>IK24</td><td>9/5 PCR p Rev,ter</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 15sec<br>55.0°C : 15sec<br>72.0°C : 30sec<br>72.0°C : 2min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: 5.0<br>Gel extraction: 5.0</p><img src="https://static.igem.org/mediawiki/2017/0/03/IGKU_b2_097_0.jpeg"><img src="https://static.igem.org/mediawiki/2017/b/b4/IGKU_b2_097_1.jpeg">
| |
| − | <!-- IGKU_b2_097_2.jpeg -->
| |
| − | <!-- IGKU_b2_097_3.jpeg -->
| |
| − | <!-- IGKU_b2_097_4.jpeg -->
| |
| − | <!-- IGKU_b2_097_5.jpeg -->
| |
| − | <!-- IGKU_b2_097_6.jpeg -->
| |
| − | <!-- IGKU_b2_097_7.jpeg -->
| |
| − | <!-- IGKU_b2_097_8.jpeg -->
| |
| − | <!-- IGKU_b2_097_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0905" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/05</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>GPD1promoter</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>GPD1promoter</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>GPD1promoter</td></tr><tr><td>4</td><td>IK21</td><td>IK22</td><td>GPD1promoter</td></tr><tr><td>5</td><td>IK21</td><td>IK22</td><td>GPD1promoter</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2*KAPA Robust <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/1/11/IGKU_b2_100_0.jpeg">
| |
| − | <!-- IGKU_b2_100_1.jpeg -->
| |
| − | <!-- IGKU_b2_100_2.jpeg -->
| |
| − | <!-- IGKU_b2_100_3.jpeg -->
| |
| − | <!-- IGKU_b2_100_4.jpeg -->
| |
| − | <!-- IGKU_b2_100_5.jpeg -->
| |
| − | <!-- IGKU_b2_100_6.jpeg -->
| |
| − | <!-- IGKU_b2_100_7.jpeg -->
| |
| − | <!-- IGKU_b2_100_8.jpeg -->
| |
| − | <!-- IGKU_b2_100_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0905" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/05</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Fukuda</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>9</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>10</td></tr><tr><td>3</td><td>14-3-3 zeta F</td><td>14-3-3 zeta R</td><td>9</td></tr><tr><td>4</td><td>14-3-3 zeta F</td><td>14-3-3 zeta R</td><td>10</td></tr><tr><td>5</td><td>tropomyosin F</td><td>tropomyosin R</td><td>11</td></tr><tr><td>6</td><td>tropomyosin F</td><td>tropomyosin R</td><td>12</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA Fast <br>95.0°C : 3min<br>95.0°C : 15sec<br>60.0°C : 15sec<br>72.0°C : 12sec<br>72.0°C : 48sec<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_106_0.jpeg -->
| |
| − | <!-- IGKU_b2_106_1.jpeg -->
| |
| − | <!-- IGKU_b2_106_2.jpeg -->
| |
| − | <!-- IGKU_b2_106_3.jpeg -->
| |
| − | <!-- IGKU_b2_106_4.jpeg -->
| |
| − | <!-- IGKU_b2_106_5.jpeg -->
| |
| − | <!-- IGKU_b2_106_6.jpeg -->
| |
| − | <!-- IGKU_b2_106_7.jpeg -->
| |
| − | <!-- IGKU_b2_106_8.jpeg -->
| |
| − | <!-- IGKU_b2_106_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0905" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/05</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Fukuda</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>tropomyosin F</td><td>tropomyosin R</td><td>11</td></tr><tr><td>2</td><td>tropomyosin F</td><td>tropomyosin R</td><td>11</td></tr><tr><td>3</td><td>tropomyosin F</td><td>tropomyosin R</td><td>11</td></tr><tr><td>4</td><td>tropomyosin F</td><td>tropomyosin R</td><td>12</td></tr><tr><td>5</td><td>tropomyosin F</td><td>tropomyosin R</td><td>12</td></tr><tr><td>6</td><td>tropomyosin F</td><td>tropomyosin R</td><td>12</td></tr><tr><td>7</td><td>14-3-3 pro F</td><td>14-3-3 pro R</td><td>13</td></tr><tr><td>8</td><td>14-3-3 pro F</td><td>14-3-3 pro R</td><td>13</td></tr><tr><td>9</td><td>14-3-3 pro F</td><td>14-3-3 pro R</td><td>13</td></tr><tr><td>10</td><td>14-3-3 pro F</td><td>14-3-3 pro R</td><td>14</td></tr><tr><td>11</td><td>14-3-3 pro F</td><td>14-3-3 pro R</td><td>14</td></tr><tr><td>12</td><td>14-3-3 pro F</td><td>14-3-3 pro R</td><td>14</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA Fast <br>95.0°C : 3min<br>95.0°C : 15sec<br>60.0°C : 15sec<br>72.0°C : 12sec<br>72.0°C : 48sec<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/a/ac/IGKU_b2_107_0.jpeg">
| |
| − | <!-- IGKU_b2_107_1.jpeg -->
| |
| − | <!-- IGKU_b2_107_2.jpeg -->
| |
| − | <!-- IGKU_b2_107_3.jpeg -->
| |
| − | <!-- IGKU_b2_107_4.jpeg -->
| |
| − | <!-- IGKU_b2_107_5.jpeg -->
| |
| − | <!-- IGKU_b2_107_6.jpeg -->
| |
| − | <!-- IGKU_b2_107_7.jpeg -->
| |
| − | <!-- IGKU_b2_107_8.jpeg -->
| |
| − | <!-- IGKU_b2_107_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0905" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/05</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Fukuda</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>tropomyosin F</td><td>tropomyosin R</td><td>11</td></tr><tr><td>2</td><td>tropomyosin F</td><td>tropomyosin R</td><td>11</td></tr><tr><td>3</td><td>tropomyosin F</td><td>tropomyosin R</td><td>11</td></tr><tr><td>4</td><td>tropomyosin F</td><td>tropomyosin R</td><td>12</td></tr><tr><td>5</td><td>tropomyosin F</td><td>tropomyosin R</td><td>12</td></tr><tr><td>6</td><td>tropomyosin F</td><td>tropomyosin R</td><td>12</td></tr><tr><td>7</td><td>eef-1g F</td><td>eef-1g R</td><td>4</td></tr><tr><td>8</td><td>AK1 F</td><td>AK1 R</td><td>5</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>9</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>9</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>9</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>9</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>10</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>10</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>10</td></tr><tr><td>16</td><td>M13F</td><td>M13R</td><td>10</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA Fast <br>95.0°C : 3min<br>95.0°C : 15sec<br>60.0°C : 15sec<br>72.0°C : 12sec<br>72.0°C : 48sec<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/c/cd/IGKU_b2_108_0.jpeg">
| |
| − | <!-- IGKU_b2_108_1.jpeg -->
| |
| − | <!-- IGKU_b2_108_2.jpeg -->
| |
| − | <!-- IGKU_b2_108_3.jpeg -->
| |
| − | <!-- IGKU_b2_108_4.jpeg -->
| |
| − | <!-- IGKU_b2_108_5.jpeg -->
| |
| − | <!-- IGKU_b2_108_6.jpeg -->
| |
| − | <!-- IGKU_b2_108_7.jpeg -->
| |
| − | <!-- IGKU_b2_108_8.jpeg -->
| |
| − | <!-- IGKU_b2_108_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0906" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/06</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>gfpp</td><td>5.0</td><td>EcoRI</td><td>2.0</td><td>None</td><td>None</td><td>H</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>gfpp</td><td>5.0</td><td>Not1</td><td>1.0</td><td>HindⅢ</td><td>1.0</td><td>K</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/b/be/IGKU_b2_098_0.jpeg">
| |
| − | <!-- IGKU_b2_098_1.jpeg -->
| |
| − | <!-- IGKU_b2_098_2.jpeg -->
| |
| − | <!-- IGKU_b2_098_3.jpeg -->
| |
| − | <!-- IGKU_b2_098_4.jpeg -->
| |
| − | <!-- IGKU_b2_098_5.jpeg -->
| |
| − | <!-- IGKU_b2_098_6.jpeg -->
| |
| − | <!-- IGKU_b2_098_7.jpeg -->
| |
| − | <!-- IGKU_b2_098_8.jpeg -->
| |
| − | <!-- IGKU_b2_098_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0906" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/06</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>163-1</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>163-2</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>164</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>142</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 20sec<br>61.0°C : 20sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/2/2c/IGKU_b2_099_0.jpeg">
| |
| − | <!-- IGKU_b2_099_1.jpeg -->
| |
| − | <!-- IGKU_b2_099_2.jpeg -->
| |
| − | <!-- IGKU_b2_099_3.jpeg -->
| |
| − | <!-- IGKU_b2_099_4.jpeg -->
| |
| − | <!-- IGKU_b2_099_5.jpeg -->
| |
| − | <!-- IGKU_b2_099_6.jpeg -->
| |
| − | <!-- IGKU_b2_099_7.jpeg -->
| |
| − | <!-- IGKU_b2_099_8.jpeg -->
| |
| − | <!-- IGKU_b2_099_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0906" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/06</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK29</td><td>IK24</td><td>Rev+t</td></tr><tr><td>2</td><td>IK29</td><td>IK24</td><td>Rev+t</td></tr><tr><td>3</td><td>IK29</td><td>IK24</td><td>Rev+t</td></tr><tr><td>4</td><td>IK29</td><td>IK24</td><td>Rev+t</td></tr><tr><td>5</td><td>IK21</td><td>IK24</td><td>Rev+t, pro PCRp</td></tr><tr><td>6</td><td>IK29</td><td>IK30</td><td>Rev7(plasmid)</td></tr><tr><td>7</td><td>IK29</td><td>IK30</td><td>Rev7(plasmid)</td></tr><tr><td>8</td><td>IK29</td><td>IK30</td><td>Rev7(plasmid)</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 15sec<br>55.0°C : 15sec<br>72.0°C : 30sec<br>72.0°C : 2min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/d/d8/IGKU_b2_102_0.jpeg">
| |
| − | <!-- IGKU_b2_102_1.jpeg -->
| |
| − | <!-- IGKU_b2_102_2.jpeg -->
| |
| − | <!-- IGKU_b2_102_3.jpeg -->
| |
| − | <!-- IGKU_b2_102_4.jpeg -->
| |
| − | <!-- IGKU_b2_102_5.jpeg -->
| |
| − | <!-- IGKU_b2_102_6.jpeg -->
| |
| − | <!-- IGKU_b2_102_7.jpeg -->
| |
| − | <!-- IGKU_b2_102_8.jpeg -->
| |
| − | <!-- IGKU_b2_102_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0906" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/06</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Dou</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>P1</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>P1</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>P1</td></tr><tr><td>4</td><td>IK21</td><td>IK22</td><td>P1</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2X KAPA Robust <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/9/9c/IGKU_b2_103_0.jpeg">
| |
| − | <!-- IGKU_b2_103_1.jpeg -->
| |
| − | <!-- IGKU_b2_103_2.jpeg -->
| |
| − | <!-- IGKU_b2_103_3.jpeg -->
| |
| − | <!-- IGKU_b2_103_4.jpeg -->
| |
| − | <!-- IGKU_b2_103_5.jpeg -->
| |
| − | <!-- IGKU_b2_103_6.jpeg -->
| |
| − | <!-- IGKU_b2_103_7.jpeg -->
| |
| − | <!-- IGKU_b2_103_8.jpeg -->
| |
| − | <!-- IGKU_b2_103_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0907" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/07</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Dou</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>p+Rev</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>p+Rev</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2X KAPA Robust <br>95.0°C : 3min<br>95.0°C : 15sec<br>55.0°C : 15sec<br>72.0°C : 1min<br>72.0°C : 1min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/7/7f/IGKU_b2_104_0.jpeg">
| |
| − | <!-- IGKU_b2_104_1.jpeg -->
| |
| − | <!-- IGKU_b2_104_2.jpeg -->
| |
| − | <!-- IGKU_b2_104_3.jpeg -->
| |
| − | <!-- IGKU_b2_104_4.jpeg -->
| |
| − | <!-- IGKU_b2_104_5.jpeg -->
| |
| − | <!-- IGKU_b2_104_6.jpeg -->
| |
| − | <!-- IGKU_b2_104_7.jpeg -->
| |
| − | <!-- IGKU_b2_104_8.jpeg -->
| |
| − | <!-- IGKU_b2_104_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0907" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/07</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK60</td><td>M13R</td><td>142 PCR</td></tr><tr><td>2</td><td>IK60</td><td>M13R</td><td>142-1</td></tr><tr><td>3</td><td>IK60</td><td>M13R</td><td>142-2</td></tr><tr><td>4</td><td>IK60</td><td>M13R</td><td>142-3</td></tr><tr><td>5</td><td>IK60</td><td>M13R</td><td>142-4</td></tr><tr><td>6</td><td>IK60</td><td>M13R</td><td>142-5</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 15sec<br>60.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/e/ef/IGKU_b2_105_0.jpeg">
| |
| − | <!-- IGKU_b2_105_1.jpeg -->
| |
| − | <!-- IGKU_b2_105_2.jpeg -->
| |
| − | <!-- IGKU_b2_105_3.jpeg -->
| |
| − | <!-- IGKU_b2_105_4.jpeg -->
| |
| − | <!-- IGKU_b2_105_5.jpeg -->
| |
| − | <!-- IGKU_b2_105_6.jpeg -->
| |
| − | <!-- IGKU_b2_105_7.jpeg -->
| |
| − | <!-- IGKU_b2_105_8.jpeg -->
| |
| − | <!-- IGKU_b2_105_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0907" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/07</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Fukuda</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK61</td><td>IK62</td><td>K733018</td></tr><tr><td>2</td><td>IK61</td><td>IK62</td><td>K731250</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA Robust <br>95.0°C : 3min<br>95.0°C : 15sec<br>60.0°C : 15sec<br>72.0°C : 15sec<br>72.0°C : 3min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/c/c8/IGKU_b2_110_0.jpeg">
| |
| − | <!-- IGKU_b2_110_1.jpeg -->
| |
| − | <!-- IGKU_b2_110_2.jpeg -->
| |
| − | <!-- IGKU_b2_110_3.jpeg -->
| |
| − | <!-- IGKU_b2_110_4.jpeg -->
| |
| − | <!-- IGKU_b2_110_5.jpeg -->
| |
| − | <!-- IGKU_b2_110_6.jpeg -->
| |
| − | <!-- IGKU_b2_110_7.jpeg -->
| |
| − | <!-- IGKU_b2_110_8.jpeg -->
| |
| − | <!-- IGKU_b2_110_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0907" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/07</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Dou</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>P1</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>P1</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>P1</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2X KAPA Robust <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/f/f9/IGKU_b2_111_0.jpeg">
| |
| − | <!-- IGKU_b2_111_1.jpeg -->
| |
| − | <!-- IGKU_b2_111_2.jpeg -->
| |
| − | <!-- IGKU_b2_111_3.jpeg -->
| |
| − | <!-- IGKU_b2_111_4.jpeg -->
| |
| − | <!-- IGKU_b2_111_5.jpeg -->
| |
| − | <!-- IGKU_b2_111_6.jpeg -->
| |
| − | <!-- IGKU_b2_111_7.jpeg -->
| |
| − | <!-- IGKU_b2_111_8.jpeg -->
| |
| − | <!-- IGKU_b2_111_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0908" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/08</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>141</td><td>5.0</td><td>XbaI</td><td>1.0</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>144</td><td>5.0</td><td>XbaI</td><td>1.0</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>143</td><td>5.0</td><td>XbaI</td><td>1.0</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>145</td><td>5.0</td><td>XbaI</td><td>1.0</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_112_0.jpeg -->
| |
| − | <!-- IGKU_b2_112_1.jpeg -->
| |
| − | <!-- IGKU_b2_112_2.jpeg -->
| |
| − | <!-- IGKU_b2_112_3.jpeg -->
| |
| − | <!-- IGKU_b2_112_4.jpeg -->
| |
| − | <!-- IGKU_b2_112_5.jpeg -->
| |
| − | <!-- IGKU_b2_112_6.jpeg -->
| |
| − | <!-- IGKU_b2_112_7.jpeg -->
| |
| − | <!-- IGKU_b2_112_8.jpeg -->
| |
| − | <!-- IGKU_b2_112_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0908" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/08</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK23</td><td>IK28</td><td>Rev3</td></tr><tr><td>2</td><td>IK23</td><td>IK28</td><td>Rev4</td></tr><tr><td>3</td><td>IK23</td><td>IK28</td><td>2(nega)</td></tr><tr><td>4</td><td>IK23</td><td>IK28</td><td>Rev new</td></tr><tr><td>5</td><td>IK29</td><td>IK30</td><td>Rev3</td></tr><tr><td>6</td><td>IK29</td><td>IK30</td><td>Rev4</td></tr><tr><td>7</td><td>IK29</td><td>IK30</td><td>2(nega)</td></tr><tr><td>8</td><td>IK29</td><td>IK30</td><td>Rev new</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA Robust <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/5/54/IGKU_b2_113_0.jpeg">
| |
| − | <!-- IGKU_b2_113_1.jpeg -->
| |
| − | <!-- IGKU_b2_113_2.jpeg -->
| |
| − | <!-- IGKU_b2_113_3.jpeg -->
| |
| − | <!-- IGKU_b2_113_4.jpeg -->
| |
| − | <!-- IGKU_b2_113_5.jpeg -->
| |
| − | <!-- IGKU_b2_113_6.jpeg -->
| |
| − | <!-- IGKU_b2_113_7.jpeg -->
| |
| − | <!-- IGKU_b2_113_8.jpeg -->
| |
| − | <!-- IGKU_b2_113_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0908" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/08</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Dou</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>p(blue font)</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>p(blue font)</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>p(blue font)</td></tr><tr><td>4</td><td>IK21</td><td>IK22</td><td>p(blue font)</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2X KAPA Robust <br>98.0°C : 3min<br>98.0°C : 20sec<br>60.0°C : 30sec<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/7/76/IGKU_b2_114_0.jpeg">
| |
| − | <!-- IGKU_b2_114_1.jpeg -->
| |
| − | <!-- IGKU_b2_114_2.jpeg -->
| |
| − | <!-- IGKU_b2_114_3.jpeg -->
| |
| − | <!-- IGKU_b2_114_4.jpeg -->
| |
| − | <!-- IGKU_b2_114_5.jpeg -->
| |
| − | <!-- IGKU_b2_114_6.jpeg -->
| |
| − | <!-- IGKU_b2_114_7.jpeg -->
| |
| − | <!-- IGKU_b2_114_8.jpeg -->
| |
| − | <!-- IGKU_b2_114_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0908" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/08</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK23</td><td>IK24</td><td>99(nega)</td></tr><tr><td>2</td><td>IK23</td><td>IK24</td><td>101</td></tr><tr><td>3</td><td>IK23</td><td>IK24</td><td>Rev3</td></tr><tr><td>4</td><td>IK23</td><td>IK24</td><td>Rev4</td></tr><tr><td>5</td><td>IK23</td><td>IK24</td><td>Rev5</td></tr><tr><td>6</td><td>IK23</td><td>IK24</td><td>Rev6</td></tr><tr><td>7</td><td>IK23</td><td>IK24</td><td>T(posi)</td></tr><tr><td>8</td><td>IK29</td><td>IK30</td><td>99</td></tr><tr><td>9</td><td>IK29</td><td>IK30</td><td>Rev5</td></tr><tr><td>10</td><td>IK29</td><td>IK30</td><td>Rev6</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 3min<br>95.0°C : 15sec<br>55.0°C : 15sec<br>72.0°C : 30sec<br>72.0°C : 2min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/f/f1/IGKU_b2_115_0.jpeg">
| |
| − | <!-- IGKU_b2_115_1.jpeg -->
| |
| − | <!-- IGKU_b2_115_2.jpeg -->
| |
| − | <!-- IGKU_b2_115_3.jpeg -->
| |
| − | <!-- IGKU_b2_115_4.jpeg -->
| |
| − | <!-- IGKU_b2_115_5.jpeg -->
| |
| − | <!-- IGKU_b2_115_6.jpeg -->
| |
| − | <!-- IGKU_b2_115_7.jpeg -->
| |
| − | <!-- IGKU_b2_115_8.jpeg -->
| |
| − | <!-- IGKU_b2_115_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0908" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/08</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK24</td><td>all</td></tr><tr><td>2</td><td>IK21</td><td>IK24</td><td>all</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>promoter</td></tr><tr><td>4</td><td>IK21</td><td>IK22</td><td>promoter</td></tr><tr><td>5</td><td>IK21</td><td>IK22</td><td>promoter</td></tr><tr><td>6</td><td>IK21</td><td>IK22</td><td>promoter</td></tr><tr><td>7</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>8</td><td>IK29</td><td>IK30</td><td>Rev</td></tr><tr><td>9</td><td>IK29</td><td>IK30</td><td>nega 99</td></tr><tr><td>10</td><td>IK29</td><td>IK30</td><td>water</td></tr><tr><td>11</td><td>IK29</td><td>IK24</td><td>Rev+t</td></tr><tr><td>12</td><td>IK29</td><td>IK24</td><td>Rev+t</td></tr><tr><td>13</td><td>IK23</td><td>IK24</td><td>plasmid</td></tr><tr><td>14</td><td>IK23</td><td>IK24</td><td>nega 99</td></tr><tr><td>15</td><td>IK23</td><td>IK24</td><td>posi(T PCRp)</td></tr><tr><td>16</td><td>IK23</td><td>IK24</td><td>posi(T PCRp)</td></tr><tr><td>17</td><td>IK23</td><td>IK24</td><td>posi(T PCRp)</td></tr><tr><td>18</td><td>IK23</td><td>IK24</td><td>posi(T PCRp)</td></tr><tr><td>19</td><td>IK23</td><td>IK24</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 1min<br>72.0°C : 5min<br>10.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/b/b3/IGKU_b2_120_0.jpeg">
| |
| − | <!-- IGKU_b2_120_1.jpeg -->
| |
| − | <!-- IGKU_b2_120_2.jpeg -->
| |
| − | <!-- IGKU_b2_120_3.jpeg -->
| |
| − | <!-- IGKU_b2_120_4.jpeg -->
| |
| − | <!-- IGKU_b2_120_5.jpeg -->
| |
| − | <!-- IGKU_b2_120_6.jpeg -->
| |
| − | <!-- IGKU_b2_120_7.jpeg -->
| |
| − | <!-- IGKU_b2_120_8.jpeg -->
| |
| − | <!-- IGKU_b2_120_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0908" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/08</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>PCRp X10</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>PCRp X10</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>genome X10</td></tr><tr><td>4</td><td>IK21</td><td>IK22</td><td>genome</td></tr><tr><td>5</td><td>IK21</td><td>IK22</td><td>water</td></tr><tr><td>6</td><td>IK21</td><td>IK24</td><td>all</td></tr><tr><td>7</td><td>IK21</td><td>IK24</td><td>p(PCRp),Rev+t gel ex</td></tr><tr><td>8</td><td>IK21</td><td>IK24</td><td>8/20 PCRp,Rev+t gel ex</td></tr><tr><td>9</td><td>IK21</td><td>IK24</td><td>RA IK21X10</td></tr><tr><td>10</td><td>IK21</td><td>IK24</td><td>RA</td></tr><tr><td>11</td><td>IK21</td><td>IK24</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min<br>72.0°C : 5min<br>10.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/e/e8/IGKU_b2_121_0.jpeg">
| |
| − | <!-- IGKU_b2_121_1.jpeg -->
| |
| − | <!-- IGKU_b2_121_2.jpeg -->
| |
| − | <!-- IGKU_b2_121_3.jpeg -->
| |
| − | <!-- IGKU_b2_121_4.jpeg -->
| |
| − | <!-- IGKU_b2_121_5.jpeg -->
| |
| − | <!-- IGKU_b2_121_6.jpeg -->
| |
| − | <!-- IGKU_b2_121_7.jpeg -->
| |
| − | <!-- IGKU_b2_121_8.jpeg -->
| |
| − | <!-- IGKU_b2_121_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0909" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/09</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>143</td><td>2</td><td>141.0</td><td>2.0</td></tr><tr><td>2</td><td>145</td><td>2</td><td>144.0</td><td>2.0</td></tr><tr><td>3</td><td>143</td><td>2</td><td>144.0</td><td>2.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_118_0.jpeg -->
| |
| − | <!-- IGKU_b2_118_1.jpeg -->
| |
| − | <!-- IGKU_b2_118_2.jpeg -->
| |
| − | <!-- IGKU_b2_118_3.jpeg -->
| |
| − | <!-- IGKU_b2_118_4.jpeg -->
| |
| − | <!-- IGKU_b2_118_5.jpeg -->
| |
| − | <!-- IGKU_b2_118_6.jpeg -->
| |
| − | <!-- IGKU_b2_118_7.jpeg -->
| |
| − | <!-- IGKU_b2_118_8.jpeg -->
| |
| − | <!-- IGKU_b2_118_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0909" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/09</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>142</td><td>20.0</td><td>XbaI</td><td>4.0</td><td>NotI</td><td>2.0</td><td>K</td><td>4.0</td><td>40.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_118_0.jpeg -->
| |
| − | <!-- IGKU_b2_118_1.jpeg -->
| |
| − | <!-- IGKU_b2_118_2.jpeg -->
| |
| − | <!-- IGKU_b2_118_3.jpeg -->
| |
| − | <!-- IGKU_b2_118_4.jpeg -->
| |
| − | <!-- IGKU_b2_118_5.jpeg -->
| |
| − | <!-- IGKU_b2_118_6.jpeg -->
| |
| − | <!-- IGKU_b2_118_7.jpeg -->
| |
| − | <!-- IGKU_b2_118_8.jpeg -->
| |
| − | <!-- IGKU_b2_118_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0909" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/09</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>323</td><td>0.34</td><td>GFPp(gal1)</td><td>12.5</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_119_0.jpeg -->
| |
| − | <!-- IGKU_b2_119_1.jpeg -->
| |
| − | <!-- IGKU_b2_119_2.jpeg -->
| |
| − | <!-- IGKU_b2_119_3.jpeg -->
| |
| − | <!-- IGKU_b2_119_4.jpeg -->
| |
| − | <!-- IGKU_b2_119_5.jpeg -->
| |
| − | <!-- IGKU_b2_119_6.jpeg -->
| |
| − | <!-- IGKU_b2_119_7.jpeg -->
| |
| − | <!-- IGKU_b2_119_8.jpeg -->
| |
| − | <!-- IGKU_b2_119_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0910" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/10</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>142-3</td><td>5.0</td><td>XbaI</td><td>0.5</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>142-5</td><td>5.0</td><td>XbaI</td><td>0.5</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>161-1</td><td>5.0</td><td>XbaI</td><td>0.5</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr><tr><td>4</td><td>163-1</td><td>5.0</td><td>XbaI</td><td>0.5</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr><tr><td>5</td><td>164</td><td>5.0</td><td>XbaI</td><td>0.5</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr><tr><td>6</td><td>166</td><td>5.0</td><td>XbaI</td><td>0.5</td><td>NotI</td><td>0.5</td><td>K</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_122_0.jpeg -->
| |
| − | <!-- IGKU_b2_122_1.jpeg -->
| |
| − | <!-- IGKU_b2_122_2.jpeg -->
| |
| − | <!-- IGKU_b2_122_3.jpeg -->
| |
| − | <!-- IGKU_b2_122_4.jpeg -->
| |
| − | <!-- IGKU_b2_122_5.jpeg -->
| |
| − | <!-- IGKU_b2_122_6.jpeg -->
| |
| − | <!-- IGKU_b2_122_7.jpeg -->
| |
| − | <!-- IGKU_b2_122_8.jpeg -->
| |
| − | <!-- IGKU_b2_122_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0914" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/14</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>IK60</td><td>162</td></tr><tr><td>2</td><td>IK29</td><td>IK24</td><td>Rev</td></tr><tr><td>3</td><td>IK40</td><td>IK55</td><td>GFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min<br>95.0°C : 1min<br>58.0°C : 1min<br>72.0°C : 1min<br>72.0°C : 2min<br>10.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/9/9e/IGKU_b2_124_0.jpeg">
| |
| − | <!-- IGKU_b2_124_1.jpeg -->
| |
| − | <!-- IGKU_b2_124_2.jpeg -->
| |
| − | <!-- IGKU_b2_124_3.jpeg -->
| |
| − | <!-- IGKU_b2_124_4.jpeg -->
| |
| − | <!-- IGKU_b2_124_5.jpeg -->
| |
| − | <!-- IGKU_b2_124_6.jpeg -->
| |
| − | <!-- IGKU_b2_124_7.jpeg -->
| |
| − | <!-- IGKU_b2_124_8.jpeg -->
| |
| − | <!-- IGKU_b2_124_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0914" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/14</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>YEplac195/sma1</td><td>0.34</td><td>163.0</td><td>13.1</td></tr><tr><td>2</td><td>YEplac195/sma1</td><td>0.34</td><td>166.0</td><td>17.25</td></tr><tr><td>3</td><td>143</td><td>2</td><td>141.0</td><td>2.0</td></tr><tr><td>4</td><td>145</td><td>2</td><td>144.0</td><td>2.0</td></tr><tr><td>5</td><td>143</td><td>2</td><td>144.0</td><td>2.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b2_125_0.jpeg -->
| |
| − | <!-- IGKU_b2_125_1.jpeg -->
| |
| − | <!-- IGKU_b2_125_2.jpeg -->
| |
| − | <!-- IGKU_b2_125_3.jpeg -->
| |
| − | <!-- IGKU_b2_125_4.jpeg -->
| |
| − | <!-- IGKU_b2_125_5.jpeg -->
| |
| − | <!-- IGKU_b2_125_6.jpeg -->
| |
| − | <!-- IGKU_b2_125_7.jpeg -->
| |
| − | <!-- IGKU_b2_125_8.jpeg -->
| |
| − | <!-- IGKU_b2_125_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0914" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/14</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK21</td><td>IK22</td><td>genome</td></tr><tr><td>2</td><td>IK21</td><td>IK22</td><td>genome</td></tr><tr><td>3</td><td>IK121</td><td>IK122</td><td>genome</td></tr><tr><td>4</td><td>IK121</td><td>IK122</td><td>genome</td></tr><tr><td>5</td><td>IK129</td><td>IK130</td><td>Rev</td></tr><tr><td>6</td><td>IK129</td><td>IK130</td><td>Rev</td></tr><tr><td>7</td><td>IK129</td><td>IK130</td><td>nega 99</td></tr><tr><td>8</td><td>IK129</td><td>IK130</td><td>water</td></tr><tr><td>9</td><td>IK21</td><td>IK22</td><td>water</td></tr><tr><td>10</td><td>IK121</td><td>IK122</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : 2X primestar <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 15sec<br>72.0°C : 5sec<br>72.0°C : 3min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: 1,2<br>PCR pulification: 4,5<br>Gel extraction: 1,2<br>Gel extraction: 4,5</p><img src="https://static.igem.org/mediawiki/2017/5/5d/IGKU_b2_126_0.jpeg">
| |
| − | <!-- IGKU_b2_126_1.jpeg -->
| |
| − | <!-- IGKU_b2_126_2.jpeg -->
| |
| − | <!-- IGKU_b2_126_3.jpeg -->
| |
| − | <!-- IGKU_b2_126_4.jpeg -->
| |
| − | <!-- IGKU_b2_126_5.jpeg -->
| |
| − | <!-- IGKU_b2_126_6.jpeg -->
| |
| − | <!-- IGKU_b2_126_7.jpeg -->
| |
| − | <!-- IGKU_b2_126_8.jpeg -->
| |
| − | <!-- IGKU_b2_126_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0914" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/14</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK121</td><td>IK122</td><td>p</td></tr><tr><td>2</td><td>IK121</td><td>IK122</td><td>p</td></tr><tr><td>3</td><td>IK21</td><td>IK22</td><td>p</td></tr><tr><td>4</td><td>IK21</td><td>IK22</td><td>p</td></tr><tr><td>5</td><td>IK121</td><td>IK122</td><td>genome</td></tr><tr><td>6</td><td>IK121</td><td>IK122</td><td>genome</td></tr><tr><td>7</td><td>IK21</td><td>IK24</td><td>p1, Rev+t</td></tr><tr><td>8</td><td>IK21</td><td>IK24</td><td>p1, Rev+t</td></tr><tr><td>9</td><td>IK129</td><td>IK24</td><td>Rev new, t</td></tr><tr><td>10</td><td>IK21</td><td>IK130</td><td>p, Rev new</td></tr><tr><td>11</td><td>IK129</td><td>IK24</td><td>water</td></tr><tr><td>12</td><td>IK21</td><td>IK130</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : prime star max <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 15sec<br>72.0°C : 5sec<br>72.0°C : 3min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/2/2d/IGKU_b2_127_0.jpeg">
| |
| − | <!-- IGKU_b2_127_1.jpeg -->
| |
| − | <!-- IGKU_b2_127_2.jpeg -->
| |
| − | <!-- IGKU_b2_127_3.jpeg -->
| |
| − | <!-- IGKU_b2_127_4.jpeg -->
| |
| − | <!-- IGKU_b2_127_5.jpeg -->
| |
| − | <!-- IGKU_b2_127_6.jpeg -->
| |
| − | <!-- IGKU_b2_127_7.jpeg -->
| |
| − | <!-- IGKU_b2_127_8.jpeg -->
| |
| − | <!-- IGKU_b2_127_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0915" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK129</td><td>IK130</td><td>Rev</td></tr><tr><td>2</td><td>IK129</td><td>IK130</td><td>Rev</td></tr><tr><td>3</td><td>IK129</td><td>IK130</td><td>Rev</td></tr><tr><td>4</td><td>IK21</td><td>IK24</td><td>OP2+(Rev+t)</td></tr><tr><td>5</td><td>IK129</td><td>IK24</td><td>NR1+t</td></tr><tr><td>6</td><td>IK21</td><td>IK130</td><td>NR1+OP2</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : primestar <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 1min30sec<br>72.0°C : 3min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/9/99/IGKU_b2_128_0.jpeg">
| |
| − | <!-- IGKU_b2_128_1.jpeg -->
| |
| − | <!-- IGKU_b2_128_2.jpeg -->
| |
| − | <!-- IGKU_b2_128_3.jpeg -->
| |
| − | <!-- IGKU_b2_128_4.jpeg -->
| |
| − | <!-- IGKU_b2_128_5.jpeg -->
| |
| − | <!-- IGKU_b2_128_6.jpeg -->
| |
| − | <!-- IGKU_b2_128_7.jpeg -->
| |
| − | <!-- IGKU_b2_128_8.jpeg -->
| |
| − | <!-- IGKU_b2_128_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0915" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK129</td><td>IK24</td><td>NR1+t</td></tr><tr><td>2</td><td>IK129</td><td>IK24</td><td>NR1+t</td></tr><tr><td>3</td><td>IK129</td><td>IK24</td><td>NR1+t</td></tr><tr><td>4</td><td>IK129</td><td>IK24</td><td>NR1+t</td></tr><tr><td>5</td><td>IK129</td><td>IK24</td><td>water</td></tr><tr><td>6</td><td>K129</td><td>IK130</td><td>rev</td></tr><tr><td>7</td><td>K129</td><td>IK130</td><td>rev</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : primestar <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 1min30sec<br>72.0°C : 3min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: 1~5<br>PCR pulification: 6,7<br>Gel extraction: 1~5<br>Gel extraction: 6,7</p><img src="https://static.igem.org/mediawiki/2017/0/0e/IGKU_b2_129_0.jpeg">
| |
| − | <!-- IGKU_b2_129_1.jpeg -->
| |
| − | <!-- IGKU_b2_129_2.jpeg -->
| |
| − | <!-- IGKU_b2_129_3.jpeg -->
| |
| − | <!-- IGKU_b2_129_4.jpeg -->
| |
| − | <!-- IGKU_b2_129_5.jpeg -->
| |
| − | <!-- IGKU_b2_129_6.jpeg -->
| |
| − | <!-- IGKU_b2_129_7.jpeg -->
| |
| − | <!-- IGKU_b2_129_8.jpeg -->
| |
| − | <!-- IGKU_b2_129_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0915" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK129</td><td>IK24</td><td>RT1</td></tr><tr><td>2</td><td>IK121</td><td>IK122</td><td>genome</td></tr><tr><td>3</td><td>IK121</td><td>IK122</td><td>genome</td></tr><tr><td>4</td><td>IK121</td><td>IK122</td><td>genome</td></tr><tr><td>5</td><td>IK21</td><td>IK24</td><td>RT1+OP1</td></tr><tr><td>6</td><td>IK21</td><td>IK24</td><td>RT1+OP2</td></tr><tr><td>7</td><td>IK121</td><td>IK24</td><td>RT1+NP1</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : primestar <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 1min30sec<br>72.0°C : 3min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/6/6e/IGKU_b2_130_0.jpeg">
| |
| − | <!-- IGKU_b2_130_1.jpeg -->
| |
| − | <!-- IGKU_b2_130_2.jpeg -->
| |
| − | <!-- IGKU_b2_130_3.jpeg -->
| |
| − | <!-- IGKU_b2_130_4.jpeg -->
| |
| − | <!-- IGKU_b2_130_5.jpeg -->
| |
| − | <!-- IGKU_b2_130_6.jpeg -->
| |
| − | <!-- IGKU_b2_130_7.jpeg -->
| |
| − | <!-- IGKU_b2_130_8.jpeg -->
| |
| − | <!-- IGKU_b2_130_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0915" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK121</td><td>IK24</td><td>RT1+NP1</td></tr><tr><td>2</td><td>IK121</td><td>IK24</td><td>RT1+NP1</td></tr><tr><td>3</td><td>IK121</td><td>IK24</td><td>RT1+NP1</td></tr><tr><td>4</td><td>IK121</td><td>IK24</td><td>RT1+NP1</td></tr><tr><td>5</td><td>IK129</td><td>IK24</td><td>RT1</td></tr><tr><td>6</td><td>IK121</td><td>IK24</td><td>water</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : primestar <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 1min30sec<br>72.0°C : 3min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: 1~4<br>Gel extraction: 1~4</p><img src="https://static.igem.org/mediawiki/2017/1/11/IGKU_b2_131_0.jpeg">
| |
| − | <!-- IGKU_b2_131_1.jpeg -->
| |
| − | <!-- IGKU_b2_131_2.jpeg -->
| |
| − | <!-- IGKU_b2_131_3.jpeg -->
| |
| − | <!-- IGKU_b2_131_4.jpeg -->
| |
| − | <!-- IGKU_b2_131_5.jpeg -->
| |
| − | <!-- IGKU_b2_131_6.jpeg -->
| |
| − | <!-- IGKU_b2_131_7.jpeg -->
| |
| − | <!-- IGKU_b2_131_8.jpeg -->
| |
| − | <!-- IGKU_b2_131_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0916" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/16</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK60</td><td>M13R</td><td>161-1</td></tr><tr><td>2</td><td>IK60</td><td>M13R</td><td>161-2</td></tr><tr><td>3</td><td>IK60</td><td>M13R</td><td>163-1</td></tr><tr><td>4</td><td>IK60</td><td>M13R</td><td>163-2</td></tr><tr><td>5</td><td>IK60</td><td>M13R</td><td>163-3</td></tr><tr><td>6</td><td>IK60</td><td>M13R</td><td>163-4</td></tr><tr><td>7</td><td>IK60</td><td>M13R</td><td>166-1</td></tr><tr><td>8</td><td>IK60</td><td>M13R</td><td>166-2</td></tr><tr><td>9</td><td>IK60</td><td>M13R</td><td>166-3</td></tr><tr><td>10</td><td>IK60</td><td>M13R</td><td>166-4</td></tr><tr><td>11</td><td>IK60</td><td>M13R</td><td>166-5</td></tr><tr><td>12</td><td>IK60</td><td>M13R</td><td>166-6</td></tr><tr><td>13</td><td>IK60</td><td>M13R</td><td>162-1</td></tr><tr><td>14</td><td>IK60</td><td>M13R</td><td>162-3</td></tr><tr><td>15</td><td>IK60</td><td>M13R</td><td>161-1</td></tr><tr><td>16</td><td>IK60</td><td>M13R</td><td>165-2</td></tr><tr><td>17</td><td>IK60</td><td>M13R</td><td>165-3</td></tr><tr><td>18</td><td>IK60</td><td>M13R</td><td>165-4</td></tr><tr><td>19</td><td>IK60</td><td>M13R</td><td>165-5</td></tr><tr><td>20</td><td>IK60</td><td>M13R</td><td>167-1</td></tr><tr><td>21</td><td>IK60</td><td>M13R</td><td>167-2</td></tr><tr><td>22</td><td>IK60</td><td>M13R</td><td>167-3</td></tr><tr><td>23</td><td>M13R</td><td>IK40</td><td>Gal1GFP 1</td></tr><tr><td>24</td><td>M13R</td><td>IK40</td><td>Gal1GFP 2</td></tr><tr><td>25</td><td>M13R</td><td>IK40</td><td>Gal1GFP 3</td></tr><tr><td>26</td><td>M13R</td><td>IK40</td><td>Gal1GFP 4</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min<br>95.0°C : 1min<br>60.0°C : 1min<br>72.0°C : 2min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_132_0.jpeg -->
| |
| − | <!-- IGKU_b2_132_1.jpeg -->
| |
| − | <!-- IGKU_b2_132_2.jpeg -->
| |
| − | <!-- IGKU_b2_132_3.jpeg -->
| |
| − | <!-- IGKU_b2_132_4.jpeg -->
| |
| − | <!-- IGKU_b2_132_5.jpeg -->
| |
| − | <!-- IGKU_b2_132_6.jpeg -->
| |
| − | <!-- IGKU_b2_132_7.jpeg -->
| |
| − | <!-- IGKU_b2_132_8.jpeg -->
| |
| − | <!-- IGKU_b2_132_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0916" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/16</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>325</td><td>0.528</td><td>prt2</td><td>0.26</td></tr><tr><td>2</td><td>325</td><td>3.65</td><td>prt2</td><td>7.33</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/8/81/IGKU_b2_133_0.jpeg">
| |
| − | <!-- IGKU_b2_133_1.jpeg -->
| |
| − | <!-- IGKU_b2_133_2.jpeg -->
| |
| − | <!-- IGKU_b2_133_3.jpeg -->
| |
| − | <!-- IGKU_b2_133_4.jpeg -->
| |
| − | <!-- IGKU_b2_133_5.jpeg -->
| |
| − | <!-- IGKU_b2_133_6.jpeg -->
| |
| − | <!-- IGKU_b2_133_7.jpeg -->
| |
| − | <!-- IGKU_b2_133_8.jpeg -->
| |
| − | <!-- IGKU_b2_133_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0916" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/16</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK121</td><td>IK24</td><td>prt2</td></tr><tr><td>2</td><td>IK121</td><td>IK24</td><td>prt3</td></tr><tr><td>3</td><td>IK121</td><td>IK24</td><td>prt3</td></tr><tr><td>4</td><td>IK121</td><td>IK24</td><td>prt3</td></tr><tr><td>5</td><td>IK121</td><td>IK24</td><td>prt3</td></tr><tr><td>6</td><td>IK121</td><td>IK122</td><td>genome</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : primestar <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 10sec<br>72.0°C : 2min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: 2~5<br>Gel extraction: 2~5</p><img src="https://static.igem.org/mediawiki/2017/1/16/IGKU_b2_134_0.jpeg">
| |
| − | <!-- IGKU_b2_134_1.jpeg -->
| |
| − | <!-- IGKU_b2_134_2.jpeg -->
| |
| − | <!-- IGKU_b2_134_3.jpeg -->
| |
| − | <!-- IGKU_b2_134_4.jpeg -->
| |
| − | <!-- IGKU_b2_134_5.jpeg -->
| |
| − | <!-- IGKU_b2_134_6.jpeg -->
| |
| − | <!-- IGKU_b2_134_7.jpeg -->
| |
| − | <!-- IGKU_b2_134_8.jpeg -->
| |
| − | <!-- IGKU_b2_134_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0916" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/16</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK60</td><td>M13R</td><td>142-5</td></tr><tr><td>2</td><td>IK60</td><td>M13R</td><td>142-5</td></tr><tr><td>3</td><td>M13F</td><td>IK60</td><td>143-1</td></tr><tr><td>4</td><td>M13F</td><td>IK60</td><td>143-2</td></tr><tr><td>5</td><td>M13F</td><td>IK60</td><td>143-3</td></tr><tr><td>6</td><td>M13F</td><td>IK60</td><td>145-1</td></tr><tr><td>7</td><td>M13F</td><td>IK60</td><td>145-2</td></tr><tr><td>8</td><td>M13F</td><td>IK60</td><td>145-3</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min<br>95.0°C : 1min<br>60.0°C : 1min<br>72.0°C : 2min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/6/61/IGKU_b2_135_0.jpeg">
| |
| − | <!-- IGKU_b2_135_1.jpeg -->
| |
| − | <!-- IGKU_b2_135_2.jpeg -->
| |
| − | <!-- IGKU_b2_135_3.jpeg -->
| |
| − | <!-- IGKU_b2_135_4.jpeg -->
| |
| − | <!-- IGKU_b2_135_5.jpeg -->
| |
| − | <!-- IGKU_b2_135_6.jpeg -->
| |
| − | <!-- IGKU_b2_135_7.jpeg -->
| |
| − | <!-- IGKU_b2_135_8.jpeg -->
| |
| − | <!-- IGKU_b2_135_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0916" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/16</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>genome</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min<br>95.0°C : 1min<br>60.0°C : 1min<br>72.0°C : 2min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_136_0.jpeg -->
| |
| − | <!-- IGKU_b2_136_1.jpeg -->
| |
| − | <!-- IGKU_b2_136_2.jpeg -->
| |
| − | <!-- IGKU_b2_136_3.jpeg -->
| |
| − | <!-- IGKU_b2_136_4.jpeg -->
| |
| − | <!-- IGKU_b2_136_5.jpeg -->
| |
| − | <!-- IGKU_b2_136_6.jpeg -->
| |
| − | <!-- IGKU_b2_136_7.jpeg -->
| |
| − | <!-- IGKU_b2_136_8.jpeg -->
| |
| − | <!-- IGKU_b2_136_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0916" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/16</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK60</td><td>M13F</td><td>164-1</td></tr><tr><td>2</td><td>IK60</td><td>M13F</td><td>164-2</td></tr><tr><td>3</td><td>M13F</td><td>IK40</td><td>Gal1 GFP 1</td></tr><tr><td>4</td><td>M13F</td><td>IK40</td><td>Gal1 GFP 2</td></tr><tr><td>5</td><td>M13F</td><td>IK40</td><td>Gal1 GFP 3</td></tr><tr><td>6</td><td>M13F</td><td>IK40</td><td>Gal1 GFP 4</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min<br>95.0°C : 1min<br>60.0°C : 1min<br>72.0°C : 1min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/1/1f/IGKU_b2_137_0.jpeg">
| |
| − | <!-- IGKU_b2_137_1.jpeg -->
| |
| − | <!-- IGKU_b2_137_2.jpeg -->
| |
| − | <!-- IGKU_b2_137_3.jpeg -->
| |
| − | <!-- IGKU_b2_137_4.jpeg -->
| |
| − | <!-- IGKU_b2_137_5.jpeg -->
| |
| − | <!-- IGKU_b2_137_6.jpeg -->
| |
| − | <!-- IGKU_b2_137_7.jpeg -->
| |
| − | <!-- IGKU_b2_137_8.jpeg -->
| |
| − | <!-- IGKU_b2_137_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0917" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/17</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK121'</td><td>IK24</td><td>N(blue colony)</td></tr><tr><td>2</td><td>IK121'</td><td>IK24</td><td>1(Rev white)</td></tr><tr><td>3</td><td>IK121'</td><td>IK24</td><td>2</td></tr><tr><td>4</td><td>IK121'</td><td>IK24</td><td>3</td></tr><tr><td>5</td><td>IK121'</td><td>IK24</td><td>prt3</td></tr><tr><td>6</td><td>IK121'</td><td>IK122'</td><td>genome</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : prime star <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 10sec<br>72.0°C : 2min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_139_0.jpeg -->
| |
| − | <!-- IGKU_b2_139_1.jpeg -->
| |
| − | <!-- IGKU_b2_139_2.jpeg -->
| |
| − | <!-- IGKU_b2_139_3.jpeg -->
| |
| − | <!-- IGKU_b2_139_4.jpeg -->
| |
| − | <!-- IGKU_b2_139_5.jpeg -->
| |
| − | <!-- IGKU_b2_139_6.jpeg -->
| |
| − | <!-- IGKU_b2_139_7.jpeg -->
| |
| − | <!-- IGKU_b2_139_8.jpeg -->
| |
| − | <!-- IGKU_b2_139_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0917" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/17</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>172-0</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>172-1</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>172-2</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>172-3</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>172-4</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>172-5</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>172-6</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>172-8</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>172-9</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>172-10</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>172-11</td></tr><tr><td>12</td><td>M13F</td><td>M13R</td><td>172-12</td></tr><tr><td>13</td><td>M13F</td><td>M13R</td><td>172-13</td></tr><tr><td>14</td><td>M13F</td><td>M13R</td><td>114-11</td></tr><tr><td>15</td><td>M13F</td><td>M13R</td><td>114-13</td></tr><tr><td>16</td><td>M13F</td><td>M13R</td><td>114-0</td></tr><tr><td>17</td><td>M13F</td><td>M13R</td><td>171-1-1</td></tr><tr><td>18</td><td>M13F</td><td>M13R</td><td>171-2</td></tr><tr><td>19</td><td>M13F</td><td>M13R</td><td>174-1</td></tr><tr><td>20</td><td>M13F</td><td>M13R</td><td>175-1</td></tr><tr><td>21</td><td>M13F</td><td>M13R</td><td>175-2</td></tr><tr><td>22</td><td>M13F</td><td>M13R</td><td>175-3</td></tr><tr><td>23</td><td>M13F</td><td>M13R</td><td>175-4</td></tr><tr><td>24</td><td>M13F</td><td>M13R</td><td>176-0</td></tr><tr><td>25</td><td>M13F</td><td>M13R</td><td>176-1</td></tr><tr><td>26</td><td>M13F</td><td>M13R</td><td>176-2</td></tr><tr><td>27</td><td>M13F</td><td>M13R</td><td>177-1</td></tr><tr><td>28</td><td>M13F</td><td>M13R</td><td>177-2</td></tr><tr><td>29</td><td>M13F</td><td>M13R</td><td>177-3</td></tr><tr><td>30</td><td>M13F</td><td>M13R</td><td>177-4</td></tr><tr><td>31</td><td>M13F</td><td>M13R</td><td>177-5</td></tr><tr><td>32</td><td>M13F</td><td>M13R</td><td>water</td></tr><tr><td>33</td><td>M13F</td><td>M13R</td><td>26(GPD AK1)</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/8/8e/IGKU_b2_140_0.jpeg">
| |
| − | <!-- IGKU_b2_140_1.jpeg -->
| |
| − | <!-- IGKU_b2_140_2.jpeg -->
| |
| − | <!-- IGKU_b2_140_3.jpeg -->
| |
| − | <!-- IGKU_b2_140_4.jpeg -->
| |
| − | <!-- IGKU_b2_140_5.jpeg -->
| |
| − | <!-- IGKU_b2_140_6.jpeg -->
| |
| − | <!-- IGKU_b2_140_7.jpeg -->
| |
| − | <!-- IGKU_b2_140_8.jpeg -->
| |
| − | <!-- IGKU_b2_140_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0917" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/17</h3></span>
| |
| − | <span class="keln_exp"><h4>Ligation</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table"><tr><th>sample</th><th>Vector name</th><th>Vector amount(ul)</th><th>Insert name</th><th>Insert amount(ul)</th></tr><tr><td>1</td><td>325</td><td>0.528</td><td>prt4</td><td>11.7</td></tr>
| |
| − | </table><img src="https://static.igem.org/mediawiki/2017/9/9c/IGKU_b2_141_0.jpeg">
| |
| − | <!-- IGKU_b2_141_1.jpeg -->
| |
| − | <!-- IGKU_b2_141_2.jpeg -->
| |
| − | <!-- IGKU_b2_141_3.jpeg -->
| |
| − | <!-- IGKU_b2_141_4.jpeg -->
| |
| − | <!-- IGKU_b2_141_5.jpeg -->
| |
| − | <!-- IGKU_b2_141_6.jpeg -->
| |
| − | <!-- IGKU_b2_141_7.jpeg -->
| |
| − | <!-- IGKU_b2_141_8.jpeg -->
| |
| − | <!-- IGKU_b2_141_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: fukuda -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0918" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/18</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK40</td><td>IK47</td><td>114-0</td></tr><tr><td>2</td><td>IK55</td><td>IK40</td><td>171-1</td></tr><tr><td>3</td><td>IK40</td><td>IK41</td><td>172-1</td></tr><tr><td>4</td><td>IK60</td><td>AK1F</td><td>176-0</td></tr><tr><td>5</td><td>IK60</td><td>AK1F</td><td>177-1</td></tr><tr><td>6</td><td>IK60</td><td>AK1F</td><td>177-2</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>58.0°C : 1min<br>72.0°C : 50sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_141_0.jpeg -->
| |
| − | <!-- IGKU_b2_141_1.jpeg -->
| |
| − | <!-- IGKU_b2_141_2.jpeg -->
| |
| − | <!-- IGKU_b2_141_3.jpeg -->
| |
| − | <!-- IGKU_b2_141_4.jpeg -->
| |
| − | <!-- IGKU_b2_141_5.jpeg -->
| |
| − | <!-- IGKU_b2_141_6.jpeg -->
| |
| − | <!-- IGKU_b2_141_7.jpeg -->
| |
| − | <!-- IGKU_b2_141_8.jpeg -->
| |
| − | <!-- IGKU_b2_141_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0918" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/18</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK60</td><td>M13R</td><td>104-1</td></tr><tr><td>2</td><td>IK60</td><td>M13R</td><td>104-2</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>60.0°C : 1min<br>72.0°C : 5min<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/4/45/IGKU_b2_142_0.jpeg">
| |
| − | <!-- IGKU_b2_142_1.jpeg -->
| |
| − | <!-- IGKU_b2_142_2.jpeg -->
| |
| − | <!-- IGKU_b2_142_3.jpeg -->
| |
| − | <!-- IGKU_b2_142_4.jpeg -->
| |
| − | <!-- IGKU_b2_142_5.jpeg -->
| |
| − | <!-- IGKU_b2_142_6.jpeg -->
| |
| − | <!-- IGKU_b2_142_7.jpeg -->
| |
| − | <!-- IGKU_b2_142_8.jpeg -->
| |
| − | <!-- IGKU_b2_142_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0919" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/19</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>PrimerF</td><td>PrimerR</td><td>162-1</td></tr><tr><td>2</td><td>PrimerF</td><td>PrimerR</td><td>162-1</td></tr><tr><td>3</td><td>PrimerF</td><td>PrimerR</td><td>162-1</td></tr><tr><td>4</td><td>PrimerF</td><td>PrimerR</td><td>162-1</td></tr><tr><td>5</td><td>PrimerF</td><td>PrimerR</td><td>Rev</td></tr><tr><td>6</td><td>PrimerF</td><td>PrimerR</td><td>Rev</td></tr><tr><td>7</td><td>PrimerF</td><td>PrimerR</td><td>Rev</td></tr><tr><td>8</td><td>PrimerF</td><td>PrimerR</td><td>Rev</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min15sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/5/5f/IGKU_b2_143_0.jpeg">
| |
| − | <!-- IGKU_b2_143_1.jpeg -->
| |
| − | <!-- IGKU_b2_143_2.jpeg -->
| |
| − | <!-- IGKU_b2_143_3.jpeg -->
| |
| − | <!-- IGKU_b2_143_4.jpeg -->
| |
| − | <!-- IGKU_b2_143_5.jpeg -->
| |
| − | <!-- IGKU_b2_143_6.jpeg -->
| |
| − | <!-- IGKU_b2_143_7.jpeg -->
| |
| − | <!-- IGKU_b2_143_8.jpeg -->
| |
| − | <!-- IGKU_b2_143_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0919" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/19</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>Ik29</td><td>IK30</td><td>OR1-1</td></tr><tr><td>2</td><td>Ik29</td><td>IK30</td><td>OR1-2</td></tr><tr><td>3</td><td>Ik29</td><td>IK30</td><td>OR1-3</td></tr><tr><td>4</td><td>Ik29</td><td>IK30</td><td>OR1-4</td></tr><tr><td>5</td><td>Ik29</td><td>IK30</td><td>OR1 nega</td></tr><tr><td>6</td><td>Ik29</td><td>IK30</td><td>R2</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min15sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/c/c4/IGKU_b2_144_0.jpeg">
| |
| − | <!-- IGKU_b2_144_1.jpeg -->
| |
| − | <!-- IGKU_b2_144_2.jpeg -->
| |
| − | <!-- IGKU_b2_144_3.jpeg -->
| |
| − | <!-- IGKU_b2_144_4.jpeg -->
| |
| − | <!-- IGKU_b2_144_5.jpeg -->
| |
| − | <!-- IGKU_b2_144_6.jpeg -->
| |
| − | <!-- IGKU_b2_144_7.jpeg -->
| |
| − | <!-- IGKU_b2_144_8.jpeg -->
| |
| − | <!-- IGKU_b2_144_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0919" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/19</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK60</td><td>M13R</td><td>162-1</td></tr><tr><td>2</td><td>IK60</td><td>M13R</td><td>162-2</td></tr><tr><td>3</td><td>IK60</td><td>M13R</td><td>162-3</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min15sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/1/1d/IGKU_b2_145_0.jpeg">
| |
| − | <!-- IGKU_b2_145_1.jpeg -->
| |
| − | <!-- IGKU_b2_145_2.jpeg -->
| |
| − | <!-- IGKU_b2_145_3.jpeg -->
| |
| − | <!-- IGKU_b2_145_4.jpeg -->
| |
| − | <!-- IGKU_b2_145_5.jpeg -->
| |
| − | <!-- IGKU_b2_145_6.jpeg -->
| |
| − | <!-- IGKU_b2_145_7.jpeg -->
| |
| − | <!-- IGKU_b2_145_8.jpeg -->
| |
| − | <!-- IGKU_b2_145_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0919" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/19</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK89</td><td>IK90</td><td>EGFP</td></tr><tr><td>2</td><td>IK89</td><td>IK90</td><td>EGFP</td></tr><tr><td>3</td><td>IK84</td><td>IK85</td><td>REV</td></tr><tr><td>4</td><td>IK86</td><td>IK87</td><td>REV</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min15sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: lane1,2<br>PCR pulification: lane3<br>Gel extraction: lane1,2<br>Gel extraction: lane3</p><img src="https://static.igem.org/mediawiki/2017/a/a9/IGKU_b2_146_0.jpeg">
| |
| − | <!-- IGKU_b2_146_1.jpeg -->
| |
| − | <!-- IGKU_b2_146_2.jpeg -->
| |
| − | <!-- IGKU_b2_146_3.jpeg -->
| |
| − | <!-- IGKU_b2_146_4.jpeg -->
| |
| − | <!-- IGKU_b2_146_5.jpeg -->
| |
| − | <!-- IGKU_b2_146_6.jpeg -->
| |
| − | <!-- IGKU_b2_146_7.jpeg -->
| |
| − | <!-- IGKU_b2_146_8.jpeg -->
| |
| − | <!-- IGKU_b2_146_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0920" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/20</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK40</td><td>IK55</td><td>171</td></tr><tr><td>2</td><td>IK40</td><td>IK55</td><td>171</td></tr><tr><td>3</td><td>IK40</td><td>IK55</td><td>171</td></tr><tr><td>4</td><td>IK40</td><td>IK55</td><td>171</td></tr><tr><td>5</td><td>IK40</td><td>IK55</td><td>171</td></tr><tr><td>6</td><td>IK40</td><td>IK55</td><td>171</td></tr><tr><td>7</td><td>IK40</td><td>IK60</td><td>177</td></tr><tr><td>8</td><td>IK40</td><td>IK60</td><td>177</td></tr><tr><td>9</td><td>IK40</td><td>IK41</td><td>172</td></tr><tr><td>10</td><td>IK40</td><td>IK41</td><td>172</td></tr><tr><td>11</td><td>IK40</td><td>IK41</td><td>172</td></tr><tr><td>12</td><td>IK40</td><td>IK41</td><td>172</td></tr><tr><td>13</td><td>IK40</td><td>IK41</td><td>172</td></tr><tr><td>14</td><td>IK40</td><td>IK41</td><td>172</td></tr><tr><td>15</td><td>IK40</td><td>IK60</td><td>176</td></tr><tr><td>16</td><td>IK40</td><td>IK60</td><td>176</td></tr><tr><td>17</td><td>IK46</td><td>M13F</td><td>148</td></tr><tr><td>18</td><td>IK46</td><td>M13F</td><td>148</td></tr><tr><td>19</td><td>IK46</td><td>M13F</td><td>148</td></tr><tr><td>20</td><td>IK46</td><td>M13F</td><td>148</td></tr><tr><td>21</td><td>IK46</td><td>M13F</td><td>148</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min15sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/a/ac/IGKU_b2_148_0.jpeg"><img src="https://static.igem.org/mediawiki/2017/1/18/IGKU_b2_148_1.jpeg">
| |
| − | <!-- IGKU_b2_148_2.jpeg -->
| |
| − | <!-- IGKU_b2_148_3.jpeg -->
| |
| − | <!-- IGKU_b2_148_4.jpeg -->
| |
| − | <!-- IGKU_b2_148_5.jpeg -->
| |
| − | <!-- IGKU_b2_148_6.jpeg -->
| |
| − | <!-- IGKU_b2_148_7.jpeg -->
| |
| − | <!-- IGKU_b2_148_8.jpeg -->
| |
| − | <!-- IGKU_b2_148_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0921" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/21</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>OR-1(REV plasmid)</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>OR-2</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>OR-3</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>OR-4</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>OR-5</td></tr><tr><td>6</td><td>IK23</td><td>Ik24</td><td>OR-1</td></tr><tr><td>7</td><td>IK23</td><td>Ik24</td><td>OR-2</td></tr><tr><td>8</td><td>IK23</td><td>Ik24</td><td>OR-3</td></tr><tr><td>9</td><td>IK23</td><td>Ik24</td><td>OR-4</td></tr><tr><td>10</td><td>IK23</td><td>Ik24</td><td>OR-5</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min15sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p><img src="https://static.igem.org/mediawiki/2017/c/c5/IGKU_b2_149_0.jpeg">
| |
| − | <!-- IGKU_b2_149_1.jpeg -->
| |
| − | <!-- IGKU_b2_149_2.jpeg -->
| |
| − | <!-- IGKU_b2_149_3.jpeg -->
| |
| − | <!-- IGKU_b2_149_4.jpeg -->
| |
| − | <!-- IGKU_b2_149_5.jpeg -->
| |
| − | <!-- IGKU_b2_149_6.jpeg -->
| |
| − | <!-- IGKU_b2_149_7.jpeg -->
| |
| − | <!-- IGKU_b2_149_8.jpeg -->
| |
| − | <!-- IGKU_b2_149_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0922" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK121</td><td>IK24</td><td>prt3</td></tr><tr><td>2</td><td>IK121</td><td>IK24</td><td>num99</td></tr><tr><td>3</td><td>IK121</td><td>IK24</td><td>OR1-1</td></tr><tr><td>4</td><td>IK121</td><td>IK24</td><td>OR1-2</td></tr><tr><td>5</td><td>IK121</td><td>IK24</td><td>OR1-3</td></tr><tr><td>6</td><td>IK121</td><td>IK24</td><td>OR1-5</td></tr><tr><td>7</td><td>IK121</td><td>IK24</td><td>OR1-4</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : prime star max <br>98.0°C : 3min<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 10sec<br>72.0°C : 2min<br>4.0°C : Infinate<br>X35 cycle<img src="https://static.igem.org/mediawiki/2017/b/be/IGKU_b2_150_0.jpeg"></p><img src="https://static.igem.org/mediawiki/2017/b/be/IGKU_b2_150_0.jpeg">
| |
| − | <!-- IGKU_b2_150_1.jpeg -->
| |
| − | <!-- IGKU_b2_150_2.jpeg -->
| |
| − | <!-- IGKU_b2_150_3.jpeg -->
| |
| − | <!-- IGKU_b2_150_4.jpeg -->
| |
| − | <!-- IGKU_b2_150_5.jpeg -->
| |
| − | <!-- IGKU_b2_150_6.jpeg -->
| |
| − | <!-- IGKU_b2_150_7.jpeg -->
| |
| − | <!-- IGKU_b2_150_8.jpeg -->
| |
| − | <!-- IGKU_b2_150_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="0922" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>09/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">Dou</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK89</td><td>IK90</td><td>EGFP2-1</td></tr><tr><td>2</td><td>IK89</td><td>IK90</td><td>EGFP2-2</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : go taq <br>95.0°C : 2min15sec<br>95.0°C : 1min<br>55.0°C : 1min<br>72.0°C : 2min15sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b2_152_0.jpeg -->
| |
| − | <!-- IGKU_b2_152_1.jpeg -->
| |
| − | <!-- IGKU_b2_152_2.jpeg -->
| |
| − | <!-- IGKU_b2_152_3.jpeg -->
| |
| − | <!-- IGKU_b2_152_4.jpeg -->
| |
| − | <!-- IGKU_b2_152_5.jpeg -->
| |
| − | <!-- IGKU_b2_152_6.jpeg -->
| |
| − | <!-- IGKU_b2_152_7.jpeg -->
| |
| − | <!-- IGKU_b2_152_8.jpeg -->
| |
| − | <!-- IGKU_b2_152_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- ############################################################## Tables END ########################################################## -->
| |
| − |
| |
| − |
| |
| − | <!-- ############################################################## Book3 Tables Start ################################################## -->
| |
| − |
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1004" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/04</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>Template 5</td><td>5.0</td><td>Xba</td><td>0.025</td><td>EcoR1</td><td>0.025</td><td>M</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>Template 5</td><td>5.0</td><td>Not1</td><td>0.025</td><td>EcoR1</td><td>0.025</td><td>H</td><td>1.0</td><td>10.0</td></tr><tr><td>3</td><td>Template 5</td><td>5.0</td><td>Not1</td><td>0.025</td><td>Xba1</td><td>0.025</td><td>H</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b3_008_0.jpeg -->
| |
| − | <!-- IGKU_b3_008_1.jpeg -->
| |
| − | <!-- IGKU_b3_008_2.jpeg -->
| |
| − | <!-- IGKU_b3_008_3.jpeg -->
| |
| − | <!-- IGKU_b3_008_4.jpeg -->
| |
| − | <!-- IGKU_b3_008_5.jpeg -->
| |
| − | <!-- IGKU_b3_008_6.jpeg -->
| |
| − | <!-- IGKU_b3_008_7.jpeg -->
| |
| − | <!-- IGKU_b3_008_8.jpeg -->
| |
| − | <!-- IGKU_b3_008_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1004" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/04</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD21</td><td>OD23</td><td>eGFP plasmid</td></tr><tr><td>2</td><td>OD21</td><td>OD23</td><td>eGFP plasmid</td></tr><tr><td>3</td><td>OD22</td><td>OD23</td><td>eGFP plasmid</td></tr><tr><td>4</td><td>OD22</td><td>OD23</td><td>eGFP plasmid</td></tr><tr><td>5</td><td>OD31</td><td>OD32</td><td>T7-RRE c</td></tr><tr><td>6</td><td>OD31</td><td>OD32</td><td>T7-RRE c</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : prime star max <br>98.0°C : 10sec<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 10sec<br>72.0°C : 10sec<br>15.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_009_0.jpeg -->
| |
| − | <!-- IGKU_b3_009_1.jpeg -->
| |
| − | <!-- IGKU_b3_009_2.jpeg -->
| |
| − | <!-- IGKU_b3_009_3.jpeg -->
| |
| − | <!-- IGKU_b3_009_4.jpeg -->
| |
| − | <!-- IGKU_b3_009_5.jpeg -->
| |
| − | <!-- IGKU_b3_009_6.jpeg -->
| |
| − | <!-- IGKU_b3_009_7.jpeg -->
| |
| − | <!-- IGKU_b3_009_8.jpeg -->
| |
| − | <!-- IGKU_b3_009_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1004" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/04</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>pSB1C3</td><td>15.0</td><td>Xba1</td><td>5.0</td><td>Spe1</td><td>5.0</td><td>M</td><td>12.0</td><td>100.0</td></tr><tr><td>2</td><td>pSB1C3</td><td>15.0</td><td>Xba1</td><td>5.0</td><td>Pst1</td><td>5.0</td><td>M</td><td>12.0</td><td>100.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b3_033_0.jpeg -->
| |
| − | <!-- IGKU_b3_033_1.jpeg -->
| |
| − | <!-- IGKU_b3_033_2.jpeg -->
| |
| − | <!-- IGKU_b3_033_3.jpeg -->
| |
| − | <!-- IGKU_b3_033_4.jpeg -->
| |
| − | <!-- IGKU_b3_033_5.jpeg -->
| |
| − | <!-- IGKU_b3_033_6.jpeg -->
| |
| − | <!-- IGKU_b3_033_7.jpeg -->
| |
| − | <!-- IGKU_b3_033_8.jpeg -->
| |
| − | <!-- IGKU_b3_033_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1005" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/05</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>vf </td><td>vr</td><td>Colony1~20(191)</td></tr><tr><td>2</td><td>vf </td><td>vr</td><td>Colony21~40(192)</td></tr><tr><td>3</td><td>vf </td><td>vr</td><td>Colony41~60(193)</td></tr><tr><td>4</td><td>vf </td><td>vr</td><td>Colony61~80(194)</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : prime star max <br>98.0°C : 10sec<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 10sec<br>72.0°C : 10sec<br>15.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_010_0.jpeg -->
| |
| − | <!-- IGKU_b3_010_1.jpeg -->
| |
| − | <!-- IGKU_b3_010_2.jpeg -->
| |
| − | <!-- IGKU_b3_010_3.jpeg -->
| |
| − | <!-- IGKU_b3_010_4.jpeg -->
| |
| − | <!-- IGKU_b3_010_5.jpeg -->
| |
| − | <!-- IGKU_b3_010_6.jpeg -->
| |
| − | <!-- IGKU_b3_010_7.jpeg -->
| |
| − | <!-- IGKU_b3_010_8.jpeg -->
| |
| − | <!-- IGKU_b3_010_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1005" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/05</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>template</td><td>5.0</td><td>Pst1</td><td>0.05</td><td>10xH</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b3_013_0.jpeg -->
| |
| − | <!-- IGKU_b3_013_1.jpeg -->
| |
| − | <!-- IGKU_b3_013_2.jpeg -->
| |
| − | <!-- IGKU_b3_013_3.jpeg -->
| |
| − | <!-- IGKU_b3_013_4.jpeg -->
| |
| − | <!-- IGKU_b3_013_5.jpeg -->
| |
| − | <!-- IGKU_b3_013_6.jpeg -->
| |
| − | <!-- IGKU_b3_013_7.jpeg -->
| |
| − | <!-- IGKU_b3_013_8.jpeg -->
| |
| − | <!-- IGKU_b3_013_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1006" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/06</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD41</td><td>OD42</td><td>Gal-GFP</td></tr><tr><td>2</td><td>OD41</td><td>OD43</td><td>Gal-GFP</td></tr><tr><td>3</td><td>OD41</td><td>OD44</td><td>Gal-GFP</td></tr><tr><td>4</td><td>IK61</td><td>OD52</td><td>Gal-GFP</td></tr><tr><td>5</td><td>OD53</td><td>OD54</td><td>Gal-GFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : prime star max <br>98.0°C : 10sec<br>98.0°C : 10sec<br>55.0°C : 5sec<br>72.0°C : 10sec<br>72.0°C : 10sec<br>15.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_012_0.jpeg -->
| |
| − | <!-- IGKU_b3_012_1.jpeg -->
| |
| − | <!-- IGKU_b3_012_2.jpeg -->
| |
| − | <!-- IGKU_b3_012_3.jpeg -->
| |
| − | <!-- IGKU_b3_012_4.jpeg -->
| |
| − | <!-- IGKU_b3_012_5.jpeg -->
| |
| − | <!-- IGKU_b3_012_6.jpeg -->
| |
| − | <!-- IGKU_b3_012_7.jpeg -->
| |
| − | <!-- IGKU_b3_012_8.jpeg -->
| |
| − | <!-- IGKU_b3_012_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1007" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/07</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK61</td><td>OD54</td><td>T7-RRE</td></tr><tr><td>2</td><td>IK61</td><td>OD54</td><td>fukuda GFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : pfu <br>95.0°C : 2min<br>95.0°C : 1min<br>55.0°C : 30sec<br>72.0°C : 4min<br>72.0°C : 4min<br>15.0°C : Infinate<br>X40 cycle</p>
| |
| − | <!-- IGKU_b3_014_0.jpeg -->
| |
| − | <!-- IGKU_b3_014_1.jpeg -->
| |
| − | <!-- IGKU_b3_014_2.jpeg -->
| |
| − | <!-- IGKU_b3_014_3.jpeg -->
| |
| − | <!-- IGKU_b3_014_4.jpeg -->
| |
| − | <!-- IGKU_b3_014_5.jpeg -->
| |
| − | <!-- IGKU_b3_014_6.jpeg -->
| |
| − | <!-- IGKU_b3_014_7.jpeg -->
| |
| − | <!-- IGKU_b3_014_8.jpeg -->
| |
| − | <!-- IGKU_b3_014_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1007" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/07</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD11</td><td>OD12</td><td>GPD-gfp</td></tr><tr><td>2</td><td>OD11</td><td>OD13</td><td>GPD-gfp</td></tr><tr><td>3</td><td>OD41</td><td>OD42</td><td>Gal-GFP</td></tr><tr><td>4</td><td>OD41</td><td>OD43</td><td>Gal-GFP</td></tr><tr><td>5</td><td>OD41</td><td>OD44</td><td>Gal-GFP</td></tr><tr><td>6</td><td>OD31</td><td>OD32</td><td>T7-RRE</td></tr><tr><td>7</td><td>IK61</td><td>IK54</td><td>fukuda GFP + T7RRE</td></tr><tr><td>8</td><td>IK61</td><td>IK54</td><td>fukuda GFP + T7RRE</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : pfu <br>95.0°C : 2min<br>95.0°C : 1min<br>55.0°C : 30sec<br>72.0°C : 4min<br>72.0°C : 4min<br>15.0°C : Infinate<br>X40 cycle</p>
| |
| − | <!-- IGKU_b3_015_0.jpeg -->
| |
| − | <!-- IGKU_b3_015_1.jpeg -->
| |
| − | <!-- IGKU_b3_015_2.jpeg -->
| |
| − | <!-- IGKU_b3_015_3.jpeg -->
| |
| − | <!-- IGKU_b3_015_4.jpeg -->
| |
| − | <!-- IGKU_b3_015_5.jpeg -->
| |
| − | <!-- IGKU_b3_015_6.jpeg -->
| |
| − | <!-- IGKU_b3_015_7.jpeg -->
| |
| − | <!-- IGKU_b3_015_8.jpeg -->
| |
| − | <!-- IGKU_b3_015_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1009" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/09</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>RE2 name</th><th>RE2 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>T7 RRE</td><td>15.0</td><td>Xba1</td><td>3.0</td><td>Pst1</td><td>3.0</td><td>M</td><td>3.0</td><td>30.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b3_019_0.jpeg -->
| |
| − | <!-- IGKU_b3_019_1.jpeg -->
| |
| − | <!-- IGKU_b3_019_2.jpeg -->
| |
| − | <!-- IGKU_b3_019_3.jpeg -->
| |
| − | <!-- IGKU_b3_019_4.jpeg -->
| |
| − | <!-- IGKU_b3_019_5.jpeg -->
| |
| − | <!-- IGKU_b3_019_6.jpeg -->
| |
| − | <!-- IGKU_b3_019_7.jpeg -->
| |
| − | <!-- IGKU_b3_019_8.jpeg -->
| |
| − | <!-- IGKU_b3_019_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1009" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/09</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK61</td><td>IK54</td><td>GFP+RRE</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : pfu <br>95.0°C : 2min<br>95.0°C : 1min<br>55.0°C : 30sec<br>72.0°C : 4min<br>72.0°C : 4min<br>15.0°C : Infinate<br>X40 cycle</p>
| |
| − | <!-- IGKU_b3_020_0.jpeg -->
| |
| − | <!-- IGKU_b3_020_1.jpeg -->
| |
| − | <!-- IGKU_b3_020_2.jpeg -->
| |
| − | <!-- IGKU_b3_020_3.jpeg -->
| |
| − | <!-- IGKU_b3_020_4.jpeg -->
| |
| − | <!-- IGKU_b3_020_5.jpeg -->
| |
| − | <!-- IGKU_b3_020_6.jpeg -->
| |
| − | <!-- IGKU_b3_020_7.jpeg -->
| |
| − | <!-- IGKU_b3_020_8.jpeg -->
| |
| − | <!-- IGKU_b3_020_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1009" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/09</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD41</td><td>OD42</td><td>Gal-gfp</td></tr><tr><td>2</td><td>OD41</td><td>OD44</td><td>Gal-gfp</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : pfu <br>95.0°C : 2min<br>95.0°C : 1min<br>55.0°C : 30sec<br>72.0°C : 4min<br>72.0°C : 4min<br>15.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_021_0.jpeg -->
| |
| − | <!-- IGKU_b3_021_1.jpeg -->
| |
| − | <!-- IGKU_b3_021_2.jpeg -->
| |
| − | <!-- IGKU_b3_021_3.jpeg -->
| |
| − | <!-- IGKU_b3_021_4.jpeg -->
| |
| − | <!-- IGKU_b3_021_5.jpeg -->
| |
| − | <!-- IGKU_b3_021_6.jpeg -->
| |
| − | <!-- IGKU_b3_021_7.jpeg -->
| |
| − | <!-- IGKU_b3_021_8.jpeg -->
| |
| − | <!-- IGKU_b3_021_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1012" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/12</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD61</td><td>OD12</td><td>TDH3-eGFP</td></tr><tr><td>2</td><td>OD62</td><td>OD12</td><td>TDH3-eGFP</td></tr><tr><td>3</td><td>OD63</td><td>OD12</td><td>TDH3-eGFP</td></tr><tr><td>4</td><td>OD61</td><td>OD13</td><td>TDH3-eGFP</td></tr><tr><td>5</td><td>OD62</td><td>OD13</td><td>TDH3-eGFP</td></tr><tr><td>6</td><td>OD63</td><td>OD13</td><td>TDH3-eGFP</td></tr><tr><td>7</td><td>OD73</td><td>IK62</td><td>GFP</td></tr><tr><td>8</td><td>OD45</td><td>OD42</td><td>GFP</td></tr><tr><td>9</td><td>OD45</td><td>OD43</td><td>GFP</td></tr><tr><td>10</td><td>OD45</td><td>OD44</td><td>GFP</td></tr><tr><td>11</td><td>IK61</td><td>OD74</td><td>GFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA HiFi fast <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_025_0.jpeg -->
| |
| − | <!-- IGKU_b3_025_1.jpeg -->
| |
| − | <!-- IGKU_b3_025_2.jpeg -->
| |
| − | <!-- IGKU_b3_025_3.jpeg -->
| |
| − | <!-- IGKU_b3_025_4.jpeg -->
| |
| − | <!-- IGKU_b3_025_5.jpeg -->
| |
| − | <!-- IGKU_b3_025_6.jpeg -->
| |
| − | <!-- IGKU_b3_025_7.jpeg -->
| |
| − | <!-- IGKU_b3_025_8.jpeg -->
| |
| − | <!-- IGKU_b3_025_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1012" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/12</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">Yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>Template14</td><td>5.0</td><td>Xba1</td><td>1.0</td><td>M</td><td>1.0</td><td>10.0</td></tr><tr><td>2</td><td>Template100</td><td>5.0</td><td>Xba1</td><td>1.0</td><td>M</td><td>1.0</td><td>10.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b3_027_0.jpeg -->
| |
| − | <!-- IGKU_b3_027_1.jpeg -->
| |
| − | <!-- IGKU_b3_027_2.jpeg -->
| |
| − | <!-- IGKU_b3_027_3.jpeg -->
| |
| − | <!-- IGKU_b3_027_4.jpeg -->
| |
| − | <!-- IGKU_b3_027_5.jpeg -->
| |
| − | <!-- IGKU_b3_027_6.jpeg -->
| |
| − | <!-- IGKU_b3_027_7.jpeg -->
| |
| − | <!-- IGKU_b3_027_8.jpeg -->
| |
| − | <!-- IGKU_b3_027_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1012" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/12</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD62</td><td>OD12</td><td>yeast genome</td></tr><tr><td>2</td><td>OD62</td><td>OD13</td><td>yeast genome</td></tr><tr><td>3</td><td>OD73</td><td>IK62</td><td>fukuda gfp</td></tr><tr><td>4</td><td>OD45</td><td>OD42</td><td>gal gfp</td></tr><tr><td>5</td><td>IK61</td><td>OD74</td><td>fukuda gfp</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_028_0.jpeg -->
| |
| − | <!-- IGKU_b3_028_1.jpeg -->
| |
| − | <!-- IGKU_b3_028_2.jpeg -->
| |
| − | <!-- IGKU_b3_028_3.jpeg -->
| |
| − | <!-- IGKU_b3_028_4.jpeg -->
| |
| − | <!-- IGKU_b3_028_5.jpeg -->
| |
| − | <!-- IGKU_b3_028_6.jpeg -->
| |
| − | <!-- IGKU_b3_028_7.jpeg -->
| |
| − | <!-- IGKU_b3_028_8.jpeg -->
| |
| − | <!-- IGKU_b3_028_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1013" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/13</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD62</td><td>OD12</td><td>yeast genome</td></tr><tr><td>2</td><td>OD62</td><td>OD12</td><td>yeast genome</td></tr><tr><td>3</td><td>OD62</td><td>OD13</td><td>TDH3-eGFP</td></tr><tr><td>4</td><td>OD62</td><td>OD13</td><td>TDH3-eGFP</td></tr><tr><td>5</td><td>OD73</td><td>IK62</td><td>fukuda GFP</td></tr><tr><td>6</td><td>IK61</td><td>OD71</td><td>fukuda GFP</td></tr><tr><td>7</td><td>OD72</td><td>OD54</td><td>T7-RRE</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_029_0.jpeg -->
| |
| − | <!-- IGKU_b3_029_1.jpeg -->
| |
| − | <!-- IGKU_b3_029_2.jpeg -->
| |
| − | <!-- IGKU_b3_029_3.jpeg -->
| |
| − | <!-- IGKU_b3_029_4.jpeg -->
| |
| − | <!-- IGKU_b3_029_5.jpeg -->
| |
| − | <!-- IGKU_b3_029_6.jpeg -->
| |
| − | <!-- IGKU_b3_029_7.jpeg -->
| |
| − | <!-- IGKU_b3_029_8.jpeg -->
| |
| − | <!-- IGKU_b3_029_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1014" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/14</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD62</td><td>OD12</td><td>yeast genome</td></tr><tr><td>2</td><td>OD62</td><td>OD13</td><td>yeast genome</td></tr><tr><td>3</td><td>OD45</td><td>OD42</td><td>gal1 GFP</td></tr><tr><td>4</td><td>IK61</td><td>OD74</td><td>fukuda GFP</td></tr><tr><td>5</td><td>OD73</td><td>IK62</td><td>fukuda GFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_032_0.jpeg -->
| |
| − | <!-- IGKU_b3_032_1.jpeg -->
| |
| − | <!-- IGKU_b3_032_2.jpeg -->
| |
| − | <!-- IGKU_b3_032_3.jpeg -->
| |
| − | <!-- IGKU_b3_032_4.jpeg -->
| |
| − | <!-- IGKU_b3_032_5.jpeg -->
| |
| − | <!-- IGKU_b3_032_6.jpeg -->
| |
| − | <!-- IGKU_b3_032_7.jpeg -->
| |
| − | <!-- IGKU_b3_032_8.jpeg -->
| |
| − | <!-- IGKU_b3_032_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: yoshimoto -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1014" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/14</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">ohara</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK103</td><td>IK104</td><td>165 gBlock</td></tr><tr><td>2</td><td>IK107</td><td>IK108</td><td>161 gBlock</td></tr><tr><td>3</td><td>IK101</td><td>IK102</td><td>161 gBlock</td></tr><tr><td>4</td><td>IK105</td><td>IK106</td><td>163 gBlock</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : yoshimoto <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_034_0.jpeg -->
| |
| − | <!-- IGKU_b3_034_1.jpeg -->
| |
| − | <!-- IGKU_b3_034_2.jpeg -->
| |
| − | <!-- IGKU_b3_034_3.jpeg -->
| |
| − | <!-- IGKU_b3_034_4.jpeg -->
| |
| − | <!-- IGKU_b3_034_5.jpeg -->
| |
| − | <!-- IGKU_b3_034_6.jpeg -->
| |
| − | <!-- IGKU_b3_034_7.jpeg -->
| |
| − | <!-- IGKU_b3_034_8.jpeg -->
| |
| − | <!-- IGKU_b3_034_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1014" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/14</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD45</td><td>OD42</td><td>gal1 GFP</td></tr><tr><td>2</td><td>IK61</td><td>OD74</td><td>fukuda GFP</td></tr><tr><td>3</td><td>OD73</td><td>IK62</td><td>fukuda GFP</td></tr><tr><td>4</td><td>IK61</td><td>OD54</td><td>fukuda GFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_035_0.jpeg -->
| |
| − | <!-- IGKU_b3_035_1.jpeg -->
| |
| − | <!-- IGKU_b3_035_2.jpeg -->
| |
| − | <!-- IGKU_b3_035_3.jpeg -->
| |
| − | <!-- IGKU_b3_035_4.jpeg -->
| |
| − | <!-- IGKU_b3_035_5.jpeg -->
| |
| − | <!-- IGKU_b3_035_6.jpeg -->
| |
| − | <!-- IGKU_b3_035_7.jpeg -->
| |
| − | <!-- IGKU_b3_035_8.jpeg -->
| |
| − | <!-- IGKU_b3_035_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1014" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/14</h3></span>
| |
| − | <span class="keln_exp"><h4>Restriction Enzyme digestion</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table"><tr><th>lane</th><th>Sample DNA name</th><th>DNA amount (ul)</th><th>RE1 name</th><th>RE1 amount(ul)</th><th>Buffer name</th><th>Buffer amount(ul)</th><th>Total volume(ul)</th></tr><tr><td>1</td><td>template</td><td>15.0</td><td>Xba1</td><td>3.0</td><td>M</td><td>3.0</td><td>100.0</td></tr><tr><td>2</td><td>template</td><td>15.0</td><td>Xba1</td><td>5.0</td><td>M</td><td>3.0</td><td>100.0</td></tr>
| |
| − | </table>
| |
| − | <!-- IGKU_b3_036_0.jpeg -->
| |
| − | <!-- IGKU_b3_036_1.jpeg -->
| |
| − | <!-- IGKU_b3_036_2.jpeg -->
| |
| − | <!-- IGKU_b3_036_3.jpeg -->
| |
| − | <!-- IGKU_b3_036_4.jpeg -->
| |
| − | <!-- IGKU_b3_036_5.jpeg -->
| |
| − | <!-- IGKU_b3_036_6.jpeg -->
| |
| − | <!-- IGKU_b3_036_7.jpeg -->
| |
| − | <!-- IGKU_b3_036_8.jpeg -->
| |
| − | <!-- IGKU_b3_036_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1014" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/14</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD31</td><td>OD32</td><td>T7+RRE</td></tr><tr><td>2</td><td>OD22</td><td>OD23</td><td>eGFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_037_0.jpeg -->
| |
| − | <!-- IGKU_b3_037_1.jpeg -->
| |
| − | <!-- IGKU_b3_037_2.jpeg -->
| |
| − | <!-- IGKU_b3_037_3.jpeg -->
| |
| − | <!-- IGKU_b3_037_4.jpeg -->
| |
| − | <!-- IGKU_b3_037_5.jpeg -->
| |
| − | <!-- IGKU_b3_037_6.jpeg -->
| |
| − | <!-- IGKU_b3_037_7.jpeg -->
| |
| − | <!-- IGKU_b3_037_8.jpeg -->
| |
| − | <!-- IGKU_b3_037_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1015" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK61</td><td>OD54</td><td>6+7</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_038_0.jpeg -->
| |
| − | <!-- IGKU_b3_038_1.jpeg -->
| |
| − | <!-- IGKU_b3_038_2.jpeg -->
| |
| − | <!-- IGKU_b3_038_3.jpeg -->
| |
| − | <!-- IGKU_b3_038_4.jpeg -->
| |
| − | <!-- IGKU_b3_038_5.jpeg -->
| |
| − | <!-- IGKU_b3_038_6.jpeg -->
| |
| − | <!-- IGKU_b3_038_7.jpeg -->
| |
| − | <!-- IGKU_b3_038_8.jpeg -->
| |
| − | <!-- IGKU_b3_038_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1015" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK85</td><td>IK86</td><td>rev</td></tr><tr><td>2</td><td>IK87</td><td>Ik88</td><td>rev</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle<br>PCR pulification: 1~2<br>Gel extraction: 1~2</p>
| |
| − | <!-- IGKU_b3_039_0.jpeg -->
| |
| − | <!-- IGKU_b3_039_1.jpeg -->
| |
| − | <!-- IGKU_b3_039_2.jpeg -->
| |
| − | <!-- IGKU_b3_039_3.jpeg -->
| |
| − | <!-- IGKU_b3_039_4.jpeg -->
| |
| − | <!-- IGKU_b3_039_5.jpeg -->
| |
| − | <!-- IGKU_b3_039_6.jpeg -->
| |
| − | <!-- IGKU_b3_039_7.jpeg -->
| |
| − | <!-- IGKU_b3_039_8.jpeg -->
| |
| − | <!-- IGKU_b3_039_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1015" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD31</td><td>OD32</td><td>T7+RRE</td></tr><tr><td>2</td><td>OD22</td><td>OD23</td><td>TDH3promoter</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_040_0.jpeg -->
| |
| − | <!-- IGKU_b3_040_1.jpeg -->
| |
| − | <!-- IGKU_b3_040_2.jpeg -->
| |
| − | <!-- IGKU_b3_040_3.jpeg -->
| |
| − | <!-- IGKU_b3_040_4.jpeg -->
| |
| − | <!-- IGKU_b3_040_5.jpeg -->
| |
| − | <!-- IGKU_b3_040_6.jpeg -->
| |
| − | <!-- IGKU_b3_040_7.jpeg -->
| |
| − | <!-- IGKU_b3_040_8.jpeg -->
| |
| − | <!-- IGKU_b3_040_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1015" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD31</td><td>OD32</td><td>T7+RRE</td></tr><tr><td>2</td><td>OD22</td><td>OD23</td><td>TDH3promoter</td></tr><tr><td>3</td><td>OD45</td><td>OD42</td><td>Gal eGFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
| |
| − | <!-- IGKU_b3_042_0.jpeg -->
| |
| − | <!-- IGKU_b3_042_1.jpeg -->
| |
| − | <!-- IGKU_b3_042_2.jpeg -->
| |
| − | <!-- IGKU_b3_042_3.jpeg -->
| |
| − | <!-- IGKU_b3_042_4.jpeg -->
| |
| − | <!-- IGKU_b3_042_5.jpeg -->
| |
| − | <!-- IGKU_b3_042_6.jpeg -->
| |
| − | <!-- IGKU_b3_042_7.jpeg -->
| |
| − | <!-- IGKU_b3_042_8.jpeg -->
| |
| − | <!-- IGKU_b3_042_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1015" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK105</td><td>Ik106</td><td>Gblock 163</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_043_0.jpeg -->
| |
| − | <!-- IGKU_b3_043_1.jpeg -->
| |
| − | <!-- IGKU_b3_043_2.jpeg -->
| |
| − | <!-- IGKU_b3_043_3.jpeg -->
| |
| − | <!-- IGKU_b3_043_4.jpeg -->
| |
| − | <!-- IGKU_b3_043_5.jpeg -->
| |
| − | <!-- IGKU_b3_043_6.jpeg -->
| |
| − | <!-- IGKU_b3_043_7.jpeg -->
| |
| − | <!-- IGKU_b3_043_8.jpeg -->
| |
| − | <!-- IGKU_b3_043_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1015" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK105</td><td>IK108</td><td>Gal1 loop</td></tr><tr><td>2</td><td>IK101</td><td>Ik104</td><td>GFP loop</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X35 cycle</p>
| |
| − | <!-- IGKU_b3_044_0.jpeg -->
| |
| − | <!-- IGKU_b3_044_1.jpeg -->
| |
| − | <!-- IGKU_b3_044_2.jpeg -->
| |
| − | <!-- IGKU_b3_044_3.jpeg -->
| |
| − | <!-- IGKU_b3_044_4.jpeg -->
| |
| − | <!-- IGKU_b3_044_5.jpeg -->
| |
| − | <!-- IGKU_b3_044_6.jpeg -->
| |
| − | <!-- IGKU_b3_044_7.jpeg -->
| |
| − | <!-- IGKU_b3_044_8.jpeg -->
| |
| − | <!-- IGKU_b3_044_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1015" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/15</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK85</td><td>IK86</td><td>Rev parts</td></tr><tr><td>2</td><td>IK87</td><td>IK88</td><td>Rev parts</td></tr><tr><td>3</td><td>IK61</td><td>OD54</td><td>T7-RRE-GFP</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
| |
| − | <!-- IGKU_b3_045_0.jpeg -->
| |
| − | <!-- IGKU_b3_045_1.jpeg -->
| |
| − | <!-- IGKU_b3_045_2.jpeg -->
| |
| − | <!-- IGKU_b3_045_3.jpeg -->
| |
| − | <!-- IGKU_b3_045_4.jpeg -->
| |
| − | <!-- IGKU_b3_045_5.jpeg -->
| |
| − | <!-- IGKU_b3_045_6.jpeg -->
| |
| − | <!-- IGKU_b3_045_7.jpeg -->
| |
| − | <!-- IGKU_b3_045_8.jpeg -->
| |
| − | <!-- IGKU_b3_045_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1017" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/17</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshitomo</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK61</td><td>OD54</td><td>4</td></tr><tr><td>2</td><td>IK61</td><td>OD54</td><td>5</td></tr><tr><td>3</td><td>IK61</td><td>OD54</td><td>7</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : KAPA hifi <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
| |
| − | <!-- IGKU_b3_048_0.jpeg -->
| |
| − | <!-- IGKU_b3_048_1.jpeg -->
| |
| − | <!-- IGKU_b3_048_2.jpeg -->
| |
| − | <!-- IGKU_b3_048_3.jpeg -->
| |
| − | <!-- IGKU_b3_048_4.jpeg -->
| |
| − | <!-- IGKU_b3_048_5.jpeg -->
| |
| − | <!-- IGKU_b3_048_6.jpeg -->
| |
| − | <!-- IGKU_b3_048_7.jpeg -->
| |
| − | <!-- IGKU_b3_048_8.jpeg -->
| |
| − | <!-- IGKU_b3_048_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1017" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/17</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK87</td><td>IK88</td><td>Rev</td></tr><tr><td>2</td><td>IK105</td><td>IK108</td><td>Gal1-loop</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : <br>95.0°C : 5min<br>98.0°C : 20sec<br>55.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
| |
| − | <!-- IGKU_b3_049_0.jpeg -->
| |
| − | <!-- IGKU_b3_049_1.jpeg -->
| |
| − | <!-- IGKU_b3_049_2.jpeg -->
| |
| − | <!-- IGKU_b3_049_3.jpeg -->
| |
| − | <!-- IGKU_b3_049_4.jpeg -->
| |
| − | <!-- IGKU_b3_049_5.jpeg -->
| |
| − | <!-- IGKU_b3_049_6.jpeg -->
| |
| − | <!-- IGKU_b3_049_7.jpeg -->
| |
| − | <!-- IGKU_b3_049_8.jpeg -->
| |
| − | <!-- IGKU_b3_049_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1018" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/18</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK105</td><td>IK108</td><td>template1</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
| |
| − | <!-- IGKU_b3_053_0.jpeg -->
| |
| − | <!-- IGKU_b3_053_1.jpeg -->
| |
| − | <!-- IGKU_b3_053_2.jpeg -->
| |
| − | <!-- IGKU_b3_053_3.jpeg -->
| |
| − | <!-- IGKU_b3_053_4.jpeg -->
| |
| − | <!-- IGKU_b3_053_5.jpeg -->
| |
| − | <!-- IGKU_b3_053_6.jpeg -->
| |
| − | <!-- IGKU_b3_053_7.jpeg -->
| |
| − | <!-- IGKU_b3_053_8.jpeg -->
| |
| − | <!-- IGKU_b3_053_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1018" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/18</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>IK107</td><td>IK108</td><td>Gal1pro-loop</td></tr><tr><td>2</td><td>IK105</td><td>IK106</td><td>Gal1pro-loop</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
| |
| − | <!-- IGKU_b3_055_0.jpeg -->
| |
| − | <!-- IGKU_b3_055_1.jpeg -->
| |
| − | <!-- IGKU_b3_055_2.jpeg -->
| |
| − | <!-- IGKU_b3_055_3.jpeg -->
| |
| − | <!-- IGKU_b3_055_4.jpeg -->
| |
| − | <!-- IGKU_b3_055_5.jpeg -->
| |
| − | <!-- IGKU_b3_055_6.jpeg -->
| |
| − | <!-- IGKU_b3_055_7.jpeg -->
| |
| − | <!-- IGKU_b3_055_8.jpeg -->
| |
| − | <!-- IGKU_b3_055_9.jpeg -->
| |
| − | </div>
| |
| − | <!------------ Table END ------------>
| |
| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1019" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/19</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD22</td><td>OD23</td><td>GPD-loop</td></tr><tr><td>2</td><td>IK87</td><td>IK88</td><td>Gal1-loop</td></tr><tr><td>3</td><td>IK105</td><td>IK108</td><td>T7-RRE</td></tr><tr><td>4</td><td>IK101</td><td>IK104</td><td>TDH3-eGFP</td></tr><tr><td>5</td><td>OD31</td><td>OD32</td><td>Rev</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
| |
| − | <!-- IGKU_b3_057_0.jpeg -->
| |
| − | <!-- IGKU_b3_057_1.jpeg -->
| |
| − | <!-- IGKU_b3_057_2.jpeg -->
| |
| − | <!-- IGKU_b3_057_3.jpeg -->
| |
| − | <!-- IGKU_b3_057_4.jpeg -->
| |
| − | <!-- IGKU_b3_057_5.jpeg -->
| |
| − | <!-- IGKU_b3_057_6.jpeg -->
| |
| − | <!-- IGKU_b3_057_7.jpeg -->
| |
| − | <!-- IGKU_b3_057_8.jpeg -->
| |
| − | <!-- IGKU_b3_057_9.jpeg -->
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| − | </div>
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| − | <!------------ Table END ------------>
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| − | <!-- Table Generated by KELN Author: ohara -->
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| − | <div class="keln_container">
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| − | <a name="1020" class = "kyoto-jump"></a>
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| − | <span class="keln_date"><h3>10/20</h3></span>
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| − | <span class="keln_exp"><h4>PCR</h4></span>
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| − | <span class="keln_researcher">yoshimoto</span>
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| − | <table class = "keln_table">
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| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>M13F</td><td>M13R</td><td>Colony 1-10 (RRE)</td></tr><tr><td>2</td><td>M13F</td><td>M13R</td><td>Colony11-17 (TDH3-eGFP)</td></tr><tr><td>3</td><td>M13F</td><td>M13R</td><td>Colony 18-21 (Rev)</td></tr><tr><td>4</td><td>M13F</td><td>M13R</td><td>Colony 22-23 (GPDp-loop)</td></tr><tr><td>5</td><td>M13F</td><td>M13R</td><td>Colony24-28 (GPDp-loop)</td></tr><tr><td>6</td><td>M13F</td><td>M13R</td><td>Colony 29-32 (T7-RRE)</td></tr><tr><td>7</td><td>M13F</td><td>M13R</td><td>Colony 33-35 (TDH3-eGFP)</td></tr><tr><td>8</td><td>M13F</td><td>M13R</td><td>Colony 36-37 (GPDp-loop)</td></tr><tr><td>9</td><td>M13F</td><td>M13R</td><td>Colony 38-40 (Gal1-loop)</td></tr><tr><td>10</td><td>M13F</td><td>M13R</td><td>Colony 41-43 (Rev)</td></tr><tr><td>11</td><td>M13F</td><td>M13R</td><td>Colony 44 (GPDp-loop)</td></tr>
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| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
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| − | <!-- IGKU_b3_058_0.jpeg -->
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| − | <!-- IGKU_b3_058_1.jpeg -->
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| − | <!-- IGKU_b3_058_2.jpeg -->
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| − | <!-- IGKU_b3_058_3.jpeg -->
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| − | <!-- IGKU_b3_058_4.jpeg -->
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| − | <!-- IGKU_b3_058_5.jpeg -->
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| − | <!-- IGKU_b3_058_6.jpeg -->
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| − | <!-- IGKU_b3_058_7.jpeg -->
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| − | <!-- IGKU_b3_058_8.jpeg -->
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| − | <!-- IGKU_b3_058_9.jpeg -->
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| − | </div>
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| − | <!------------ Table END ------------>
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| − | <!-- Table Generated by KELN Author: ohara -->
| |
| − | <div class="keln_container">
| |
| − | <a name="1022" class = "kyoto-jump"></a>
| |
| − | <span class="keln_date"><h3>10/22</h3></span>
| |
| − | <span class="keln_exp"><h4>PCR</h4></span>
| |
| − | <span class="keln_researcher">yoshimoto</span>
| |
| − | <table class = "keln_table">
| |
| − | <tr><th>lane</th><th>Primer Foward</th><th>Primer Revese</th><th>Template</th></tr><tr><td>1</td><td>OD22</td><td>OD23</td><td>TDH3 GFP</td></tr><tr><td>2</td><td>IK87</td><td>IK88</td><td>Rev</td></tr><tr><td>3</td><td>IK105</td><td>IK108</td><td>Gal1</td></tr><tr><td>4</td><td>IK101</td><td>IK104</td><td>GPD1</td></tr><tr><td>5</td><td>OD31</td><td>OD32</td><td>T7-RRE</td></tr>
| |
| − | </table>
| |
| − | <p class="keln_note">PCR Polymerase : <br>95.0°C : 5min<br>98.0°C : 20sec<br>58.0°C : 15sec<br>72.0°C : 40sec<br>72.0°C : 5min<br>4.0°C : Infinate<br>X30 cycle</p>
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| − | <!-- IGKU_b3_059_0.jpeg -->
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| − | <!-- IGKU_b3_059_1.jpeg -->
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| − | <!-- IGKU_b3_059_2.jpeg -->
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| − | <!-- IGKU_b3_059_3.jpeg -->
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| − | <!-- IGKU_b3_059_4.jpeg -->
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| − | <!-- IGKU_b3_059_5.jpeg -->
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| − | <!-- IGKU_b3_059_6.jpeg -->
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| − | <!-- IGKU_b3_059_7.jpeg -->
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| − | <!-- IGKU_b3_059_8.jpeg -->
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| − | <!-- IGKU_b3_059_9.jpeg -->
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| − | </div>
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| − | <!------------ Table END ------------>
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| | <!-- Table Generated by KELN Author: Tsuzuki --> | | <!-- Table Generated by KELN Author: Tsuzuki --> |
| | <div class="keln_container"> | | <div class="keln_container"> |
| Line 4,168: |
Line 352: |
| | <p class="keln_note">add 4µL linearized plasmid backbone (25ng/µL)<br>add 4µL Enzyme Master Mix<br>digest 37℃, 30min<br>add 8µL NaOAc (pH5.2,3M)<br>add 16µL 99.5% EtOh<br>Vortex 2sec<br>add 184µL 99.5% EtOH<br>add 72µL DW</p> | | <p class="keln_note">add 4µL linearized plasmid backbone (25ng/µL)<br>add 4µL Enzyme Master Mix<br>digest 37℃, 30min<br>add 8µL NaOAc (pH5.2,3M)<br>add 16µL 99.5% EtOh<br>Vortex 2sec<br>add 184µL 99.5% EtOH<br>add 72µL DW</p> |
| | <!------------ Table END ------------> | | <!------------ Table END ------------> |
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| − | <!-- ############################################################## Book3 Tables END ################################################## -->
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