Difference between revisions of "Team:Leiden/Attributions"

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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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  <h1 class="SectionTitle">Attributions</h1>
 +
  <p>iGEM Leiden 2018 would like to acknowledge the invaluable contributions of all those who have helped to advance our project over the past months. Without their support it would not have been possible to successfully perform our experiments in
 +
    the laboratory, promote or fundraise our project. Not only did they help in making this iGEM project possible, they have also contributed to making this experience incredibly fun! This page is dedicated to all these wonderful people and
 +
    organisations. <br><br>Our special thanks goes to:</p>
  
 +
  <h2 class="SubSectionTitle">Principle Investigators</h2>
 +
  <ul>
 +
    <li>Dennis Claessen</li>
 +
    <li>Han de Winde</li>
 +
  </ul>
  
 +
  <h2 class="SubSectionTitle">Advisors</h2>
 +
  <ul>
 +
    <li>Charlotte van de Velde</li>
 +
    <li>Sjoerd Seekles</li>
 +
    <li>Vincent Bakker</li>
 +
  </ul>
  
 +
  <h2 class="SubSectionTitle">General Support</h2>
 +
  <ul>
 +
    <li>Laura van Achteren</li>
 +
    <li class="description">For her help in creating the invoices for our sponsors</li>
 +
    <li>Hestia Tamboer & Sandra Jansen</li>
 +
    <li class="description">For their tremendous dedication and help in booking our flights to Boston
 +
    </li>
 +
    <li>Kick Moors</li>
 +
    <li class="description">For his guidance in the team role formation process
 +
    </li>
 +
    <li>Anne Maren Kruse</li>
 +
    <li class="description">For her efforts in securing working space and funding at Leiden University</li>
 +
    <li>Gerda Lamers</li>
 +
    <li class="description">For allowing us to use the DinoLite ocular for making microscopy images and for helping us with the microscopy experiments</li>
 +
  </ul>
  
 +
  <h2 class="SubSectionTitle">Experimental Support</h2>
 +
  <ul>
 +
    <li>Kees Koops</li>
 +
    <li class="description">For his help in setting up our lab space and his readiness to help us in all our (minor) crises</li>
 +
    <li>Lizah van der Aart</li>
 +
    <li class="description">For her invaluable advice on the overlay technique as well as her efforts in 3D printing keychains for our crowdfunding campaign</li>
 +
    <li>Ellen de Waal & Ilja Bekker</li>
 +
    <li class="description">For their help with ordering our lab materials from various suppliers</li>
 +
    <li>Thomas Bakkum</li>
 +
    <li class="description">For generously allowing us to use the FACS machine of his department</li>
 +
    <li>Paul van Heusden</li>
 +
    <li class="description">For his patient explanation of the FACS machine</li>
 +
    <li>Gerben Voshol</li>
 +
    <li class="description">For his advice on fixating bacteria for FACS analysis</li>
 +
    <li>iGEM Groningen 2018</li>
 +
    <li class="description">For providing us with the pNZ8040 and pMK4 plasmids for our bacillus subtilis subproject</li>
 +
    <li>iGEM Delft 2018</li>
 +
    <li class="description">For providing us with the transformed E.coli DH5α for the InterLab</li>
 +
  </ul>
  
 +
  <h2 class="SubSectionTitle">Fundraising</h2>
 +
  <ul>
 +
    <li>Annette Heijn</li>
 +
    <li class="description">For supporting us before and during our crowdfunding campaign, for her indispensable advice and her continuous assistance</li>
 +
    <li>Milou van Leeuwen</li>
 +
    <li class="description">For her dedication in making our crowdfunding video absolutely perfect</li>
 +
    <li>Corine Hendriks</li>
 +
    <li class="description">For her article about our project and crowdfunding campaign on the university website</li>
 +
    <li>Wendy Persson</li>
 +
    <li class="description">For her general assistance during the crowdfunding campaign and for troubleshooting when required</li>
 +
    <li>Roy Zitman (PLNT)</li>
 +
    <li class="description">For providing us with contacts within Leiden and leads on where to secure funding for our project</li>
 +
    <li>The Science Meets Business Foundation, in specific: <br> Max Green, Max van 't Hof, Vincent van der Wel and Coen Breedveld</li>
 +
    <li class="description">For providing the team with general advise regarding fundraising, project management and crowdfunding</li>
 +
  </ul>
  
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<div class="column full_size">
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  <h2 class="SubSectionTitle">Societal Outreach</h2>
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<h1>Attributions</h1>
+
  <ul>
−
<p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.
+
    <li>Danny Rozen</li>
−
</p>
+
    <li class="description">For allowing us to present our project at This Weeks Discovery, a university platform where scientists gather to present their current research</li>
 +
    <li>Federico Muffatto</li>
 +
    <li class="description">For providing us with the M. roseus and M. luteus strains to use for bioart in our societal outreach</li>
 +
    <li>Margot Duesmann</li>
 +
    <li class="description">For allowing us to organise a workshop during Night at the Museums in Leiden</li>
 +
    <li>Frédérique de Paus</li>
 +
    <li class="description">For coordinating our team’s involvement in Dutch festival Werfpop
 +
    </li>
 +
  </ul>
  
−
<h3> Bronze Medal Criterion #3</h3>
+
  <h2 class="SectionTitle">Team Contributions</h1>
−
<p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.  
+
  <p>This project was designed, organised and executed by members of our team. The initial idea for our project was the result of an internship completed by our Science manager Marjolein Crooijmans under our PI Dennis Claessen. Although everyone
−
<br><br>
+
    had their own role to play within the team, we helped each other in all project aspects. We credit this willingness to help each other as the most important factor in our successes.</p>
−
Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
+
−
</div>
+
  
 +
  <h2 class="SubSectionTitle">Experimental Work</h2>
 +
  <p>In our lab we benefited from a very free working atmosphere, and even though people were assigned to a specific subproject we all pitched in and helped each other where needed. <br><br>Before our project started, Marjolein worked on isolating our first 30 stress promoters and coupling them to GFP constructs to enable testing.<br><br></p>
 +
  <ul class="bullet">
 +
    <li>Charlotte de Ceuninck van Capelle and Germaine Aalderink</li>
 +
    <li class="description">Charlotte and Germaine were responsible for the selection of the bacillus subtilis promoters as well as transforming bacillus with a chromoprotein.</li>
 +
    <li>Daphne van den Homberg and Mees Fox</li>
 +
    <li class="description">Daphne created all our new promoter constructs using PCR. As a team, Daphne and Mees focussed on coupling these new promoters to GFP.</li>
 +
    <li>Maaike de Jong and Lotte Weel</li>
 +
    <li class="description">Maaike and Lotte occupied themselves with the construction of our composite plasmid.
 +
    </li>
 +
    <li>Tim de Jong and Carli Koster</li>
 +
    <li class="description">Tim and Carli were responsible for the validation of our system using fluorescence-activated cell sorting.</li>
 +
    <li>Jazzy de Waard and Marijke Grundeken</li>
 +
    <li class="description">As antibiotics are traditionally discovered with the overlay techniques using streptomyces and E.coli, Jazzy and Marijke learned to perform this technique as well as disk diffusion. </li>
 +
    <li>Laurens ter Haar and Marjolein Crooijmans</li>
 +
    <li class="description">Laurens and Marjolein were concerned with enhancing our system so we could obtain a clear read-out. To this end they used T7.</li>
 +
  </ul>
  
−
<div class="clear extra_space"></div>
+
  <h2 class="SubSectionTitle">Fundraising and Finances</h2>
 +
  <p>Tim de Jong and Jazzy de Waard functioned as our Fundraising team and were mainly responsible for approaching sponsors and managing our crowdfunding campaign. Our treasurer Maaike de Jong safeguarded our finances and managed our budget with
 +
    occasional help from Charlotte de Ceuninck van Capelle.
 +
  </p>
  
 +
  <h2 class="SubSectionTitle">Design</h2>
 +
  <p>Our secretary and design manager Daphne van den Homberg designed all of our promotion materials, including flyers, banners, postcards et cetera with help from Laurens ter Haar and Chiel van Amstel.
 +
  </p>
  
 +
  <h2 class="SubSectionTitle">Collaborations</h2>
 +
  <p>Carli Koster was responsible for the countless collaborations that we did with teams from all around the world, such as the postcards from team Düsseldorf, the Instagram Challenge for July and Moves Like iGEM amongst others. Charlotte de
 +
    Ceuninck van Capelle managed the collaboration between Leiden and Rotterdam HR as well as the Dutch Meet-Up and BBQ.
 +
  </p>
  
−
<div class="column third_size">
+
  <h2 class="SubSectionTitle">Programming and Statistics</h2>
−
<h3> What should this page contain?</h3>
+
  <p>All of our wiki programming and statistics were performed by Chiel van Amstel.
 +
  </p>
  
−
<ul>
+
  <h2 class="SubSectionTitle">Outreach and Social Media</h2>
−
<li>Clearly state what the team accomplished</li>
+
  <p>Our PR team, Carli Koster and Marijke Grundeken, managed all of our social media accounts and our monthly newsletter, while Mees Fox arranged for us to be present at local events to give workshops and talks about our project.
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<li>General Support</li>
+
  </p>
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<li>Project support and advice</li>
+
−
<li>Fundraising help and advice</li>
+
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<li>Lab support</li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
+
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<li>Wiki support</li>
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<li>Presentation coaching</li>
+
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<li>Human Practices support</li>
+
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
+
−
</ul>
+
−
</div>
+
  
−
<div class="column third_size">
+
  <h2 class="SubSectionTitle">InterLab</h2>
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
+
  <p>Charlotte de Ceuninck van Capelle arranged for iGEM Leiden to be able to use the transformed InterLab strains from team Delft. All InterLab experiments were performed by Chiel van Amstel and Jazzy de Waard.
−
<ul>
+
  </p>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
+
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<li>When did you start this course?</li>
+
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<li>Are the syllabus and course materials freely available online?</li>
+
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<li>When did you start your brainstorming?</li>
+
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<li>When did you start in the lab?</li>
+
−
<li>When did you start working on  your project?</li>
+
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</ul>
+
−
</div>
+
  
 +
  <h2 class="SubSectionTitle">Lab Safety</h2>
 +
  <p>Naturally, all of our team members took lab safety extremely seriously. However, Germaine Aalderink was responsible for arranging the GMO course everyone needed to complete in order to work in the ML-1 lab and for completing our safety form.
 +
  </p>
  
−
<div class="column third_size">
+
  <h2 class="SubSectionTitle">Presentations</h2>
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<div class="highlight decoration_A_full">
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  <p>Laurens ter Haar presented our project at events like This Week’s Discovery and the Science Meets Business Cafe.
 +
  </p>
 +
 
 +
  <h2 class="SubSectionTitle">Wiki</h2>
 +
  <p>As our programming manager Chiel programmed the entire wiki. All team members contributed to writing the wiki but the final content was managed by Laurens ter Haar.</p>
  
−
<h3>Inspiration</h3>
 
−
<p>Take a look at what other teams have done:</p>
 
−
<ul>
 
−
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
 
−
<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
 
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</ul>
 
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<h3> Why is this page needed? </h3>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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<p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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<h3> Can we base our project on a previous one? </h3>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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Revision as of 11:31, 4 September 2018

Attributions

iGEM Leiden 2018 would like to acknowledge the invaluable contributions of all those who have helped to advance our project over the past months. Without their support it would not have been possible to successfully perform our experiments in the laboratory, promote or fundraise our project. Not only did they help in making this iGEM project possible, they have also contributed to making this experience incredibly fun! This page is dedicated to all these wonderful people and organisations.

Our special thanks goes to:

Principle Investigators

  • Dennis Claessen
  • Han de Winde

Advisors

  • Charlotte van de Velde
  • Sjoerd Seekles
  • Vincent Bakker

General Support

  • Laura van Achteren
  • For her help in creating the invoices for our sponsors
  • Hestia Tamboer & Sandra Jansen
  • For their tremendous dedication and help in booking our flights to Boston
  • Kick Moors
  • For his guidance in the team role formation process
  • Anne Maren Kruse
  • For her efforts in securing working space and funding at Leiden University
  • Gerda Lamers
  • For allowing us to use the DinoLite ocular for making microscopy images and for helping us with the microscopy experiments

Experimental Support

  • Kees Koops
  • For his help in setting up our lab space and his readiness to help us in all our (minor) crises
  • Lizah van der Aart
  • For her invaluable advice on the overlay technique as well as her efforts in 3D printing keychains for our crowdfunding campaign
  • Ellen de Waal & Ilja Bekker
  • For their help with ordering our lab materials from various suppliers
  • Thomas Bakkum
  • For generously allowing us to use the FACS machine of his department
  • Paul van Heusden
  • For his patient explanation of the FACS machine
  • Gerben Voshol
  • For his advice on fixating bacteria for FACS analysis
  • iGEM Groningen 2018
  • For providing us with the pNZ8040 and pMK4 plasmids for our bacillus subtilis subproject
  • iGEM Delft 2018
  • For providing us with the transformed E.coli DH5α for the InterLab

Fundraising

  • Annette Heijn
  • For supporting us before and during our crowdfunding campaign, for her indispensable advice and her continuous assistance
  • Milou van Leeuwen
  • For her dedication in making our crowdfunding video absolutely perfect
  • Corine Hendriks
  • For her article about our project and crowdfunding campaign on the university website
  • Wendy Persson
  • For her general assistance during the crowdfunding campaign and for troubleshooting when required
  • Roy Zitman (PLNT)
  • For providing us with contacts within Leiden and leads on where to secure funding for our project
  • The Science Meets Business Foundation, in specific:
    Max Green, Max van 't Hof, Vincent van der Wel and Coen Breedveld
  • For providing the team with general advise regarding fundraising, project management and crowdfunding

Societal Outreach

  • Danny Rozen
  • For allowing us to present our project at This Weeks Discovery, a university platform where scientists gather to present their current research
  • Federico Muffatto
  • For providing us with the M. roseus and M. luteus strains to use for bioart in our societal outreach
  • Margot Duesmann
  • For allowing us to organise a workshop during Night at the Museums in Leiden
  • Frédérique de Paus
  • For coordinating our team’s involvement in Dutch festival Werfpop

Team Contributions

This project was designed, organised and executed by members of our team. The initial idea for our project was the result of an internship completed by our Science manager Marjolein Crooijmans under our PI Dennis Claessen. Although everyone had their own role to play within the team, we helped each other in all project aspects. We credit this willingness to help each other as the most important factor in our successes.

Experimental Work

In our lab we benefited from a very free working atmosphere, and even though people were assigned to a specific subproject we all pitched in and helped each other where needed.

Before our project started, Marjolein worked on isolating our first 30 stress promoters and coupling them to GFP constructs to enable testing.

  • Charlotte de Ceuninck van Capelle and Germaine Aalderink
  • Charlotte and Germaine were responsible for the selection of the bacillus subtilis promoters as well as transforming bacillus with a chromoprotein.
  • Daphne van den Homberg and Mees Fox
  • Daphne created all our new promoter constructs using PCR. As a team, Daphne and Mees focussed on coupling these new promoters to GFP.
  • Maaike de Jong and Lotte Weel
  • Maaike and Lotte occupied themselves with the construction of our composite plasmid.
  • Tim de Jong and Carli Koster
  • Tim and Carli were responsible for the validation of our system using fluorescence-activated cell sorting.
  • Jazzy de Waard and Marijke Grundeken
  • As antibiotics are traditionally discovered with the overlay techniques using streptomyces and E.coli, Jazzy and Marijke learned to perform this technique as well as disk diffusion.
  • Laurens ter Haar and Marjolein Crooijmans
  • Laurens and Marjolein were concerned with enhancing our system so we could obtain a clear read-out. To this end they used T7.

Fundraising and Finances

Tim de Jong and Jazzy de Waard functioned as our Fundraising team and were mainly responsible for approaching sponsors and managing our crowdfunding campaign. Our treasurer Maaike de Jong safeguarded our finances and managed our budget with occasional help from Charlotte de Ceuninck van Capelle.

Design

Our secretary and design manager Daphne van den Homberg designed all of our promotion materials, including flyers, banners, postcards et cetera with help from Laurens ter Haar and Chiel van Amstel.

Collaborations

Carli Koster was responsible for the countless collaborations that we did with teams from all around the world, such as the postcards from team Düsseldorf, the Instagram Challenge for July and Moves Like iGEM amongst others. Charlotte de Ceuninck van Capelle managed the collaboration between Leiden and Rotterdam HR as well as the Dutch Meet-Up and BBQ.

Programming and Statistics

All of our wiki programming and statistics were performed by Chiel van Amstel.

Outreach and Social Media

Our PR team, Carli Koster and Marijke Grundeken, managed all of our social media accounts and our monthly newsletter, while Mees Fox arranged for us to be present at local events to give workshops and talks about our project.

InterLab

Charlotte de Ceuninck van Capelle arranged for iGEM Leiden to be able to use the transformed InterLab strains from team Delft. All InterLab experiments were performed by Chiel van Amstel and Jazzy de Waard.

Lab Safety

Naturally, all of our team members took lab safety extremely seriously. However, Germaine Aalderink was responsible for arranging the GMO course everyone needed to complete in order to work in the ML-1 lab and for completing our safety form.

Presentations

Laurens ter Haar presented our project at events like This Week’s Discovery and the Science Meets Business Cafe.

Wiki

As our programming manager Chiel programmed the entire wiki. All team members contributed to writing the wiki but the final content was managed by Laurens ter Haar.