Difference between revisions of "Team:Munich/thisisatest.html"
| Line 37: | Line 37: | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td>inoculate pRED at 30°C because of temperature sensitive promoter</td> | + | <td>inoculate pRED at 30°C because of temperature sensitive promoter, pKD3 contains resistance cassette flanked by FRT sites</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 56: | Line 56: | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td>chem. Trafo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>pKD3 contains resistance cassette flanked by FRT sites</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 69: | Line 69: | ||
| + | <h3>DNA preparation for pRED/ET Engineering</h3> | ||
| + | <table class="table table-borderless"> | ||
| + | <tr> | ||
| + | |||
| + | <td>Participants:</td> | ||
| + | <td>Dominic</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Protocol:</td> | ||
| + | <td>mini prep</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Notes:</td> | ||
| + | <td>because pRED/ET could not be transformed, we got readily transformed cells from PD Dr. Jürgen Lassak from the LMU</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Results:</td> | ||
| + | <td>pRED/ET: 37,5 ng/ul | ||
| + | pNPTS138-R6KT: 60ng/ul | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
</div> | </div> | ||
Revision as of 15:04, 14 September 2018
Contents
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies |
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter, pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
DNA preparation for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | mini prep |
| Notes: | because pRED/ET could not be transformed, we got readily transformed cells from PD Dr. Jürgen Lassak from the LMU |
| Results: | pRED/ET: 37,5 ng/ul
pNPTS138-R6KT: 60ng/ul |