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− | + | <html lang="en"> | |
− | < | + | <head> |
+ | <meta charset="utf-8"> | ||
+ | <title>HillSide Multi purpose HTML5 Template</title> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0" /> | ||
+ | <meta name="description" content="" /> | ||
+ | <meta name="author" content="http://webthemez.com" /> | ||
+ | <!-- css --> | ||
+ | <link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:HUST-China/materialize/css/materialize?action=raw&ctype=text/css" media="screen,projection" /> | ||
+ | <link href="https://2018.igem.org/Team:HUST-China/css/bootstrap?action=raw&ctype=text/css" rel="stylesheet" /> | ||
+ | <link href="https://2018.igem.org/Team:HUST-China/css/fancybox/jquery?action=raw&ctype=text/css" rel="stylesheet"> | ||
+ | <link href="https://2018.igem.org/Team:HUST-China/css/flexslider?action=raw&ctype=text/css" rel="stylesheet" /> | ||
+ | <!--slider--> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:HUST-China/css/zoomslider?action=raw&ctype=text/css" /> | ||
+ | <link href="https://2018.igem.org/Team:HUST-China/css/font-awesome?action=raw&ctype=text/css" rel="stylesheet" /> | ||
+ | <link href="https://2018.igem.org/Team:HUST-China/css/animate?action=raw&ctype=text/css" rel="stylesheet" /> | ||
+ | <!--<link href="https://2018.igem.org/Team:HUST-China/css/style?action=raw&ctype=text/css" rel="stylesheet" /> --> | ||
+ | <style type="text/css"> | ||
+ | /* | ||
+ | Author URI: http://webthemez.com/ | ||
+ | Note: Licence under Creative Commons Attribution 3.0 | ||
+ | -------------------------------------------------------*/ | ||
− | + | @import url('http://fonts.googleapis.com/css?family=Noto+Serif:400,400italic,700|Open+Sans:400,600,700'); | |
− | + | @import url('font-awesome.css'); | |
+ | @import url('animate.css'); | ||
− | + | body { | |
+ | font-family:'Open Sans', Arial, sans-serif; | ||
+ | font-weight:300; | ||
+ | line-height:1.6em; | ||
+ | color:#656565; | ||
+ | font-size: 1.4em; | ||
+ | background: #ffffff; | ||
+ | } | ||
+ | a { | ||
+ | color: #0a0a0a; | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | a:active { | ||
+ | outline:0; | ||
+ | } | ||
− | + | .clear { | |
+ | clear:both; | ||
+ | } | ||
+ | h1,h2{ | ||
+ | font-weight: 600; | ||
+ | line-height:1.1em; | ||
+ | color: #e91e63; | ||
+ | margin-bottom: 20px; | ||
+ | text-transform: uppercase; | ||
+ | } | ||
+ | h3, h4, h5, h6 { | ||
+ | line-height:1.1em; | ||
+ | color: #212121; | ||
+ | margin-bottom: 20px; | ||
+ | } | ||
+ | input, button, select, textarea { | ||
+ | border-radius: 0px !important; | ||
+ | } | ||
+ | .timetable{background: #2ab975;padding: 5px 23px;} | ||
+ | .timetable hr{ | ||
+ | color:#fff; | ||
+ | padding-top: 29px; | ||
+ | margin-bottom: 20px !important; | ||
+ | } | ||
+ | .timetable dl{ | ||
+ | color:#fff; | ||
+ | } | ||
+ | .timetable dt{ | ||
+ | color:#fff; | ||
+ | } | ||
+ | .timetable dd{ | ||
+ | color:#fff; | ||
+ | } | ||
+ | #wrapper{ | ||
+ | width:100%; | ||
+ | margin:0; | ||
+ | padding:0; | ||
+ | } | ||
+ | ul.withArrow{ | ||
+ | |||
+ | } | ||
+ | ul.withArrow li{ | ||
+ | |||
+ | } | ||
+ | ul.withArrow li span{ | ||
+ | |||
+ | margin-right: 10px; | ||
+ | |||
+ | font-weight: 700; | ||
+ | |||
+ | color: #0e0e0e; | ||
+ | |||
+ | border: 1px solid; | ||
+ | |||
+ | padding: 4px 8px; | ||
+ | |||
+ | font-size: 11px; | ||
+ | |||
+ | border-radius: 50%; | ||
+ | |||
+ | height: 20px; | ||
+ | |||
+ | width: 20px; | ||
+ | } | ||
+ | .widget { | ||
+ | color: #858585; | ||
+ | } | ||
+ | .row,.row-fluid { | ||
+ | margin-bottom:30px; | ||
+ | } | ||
+ | .row .row,.row-fluid .row-fluid{ | ||
+ | margin-bottom:30px; | ||
+ | } | ||
− | + | .row.nomargin,.row-fluid.nomargin { | |
− | + | margin-bottom:0; | |
− | + | } | |
− | + | .about-image img { | |
− | + | width: 100%; | |
− | + | } | |
− | + | img.img-polaroid { | |
− | + | margin:0 0 20px 0; | |
− | + | } | |
+ | .img-box { | ||
+ | max-width:100%; | ||
+ | } | ||
+ | .navbar-default .navbar-toggle { | ||
+ | border-color: #ddd; | ||
+ | margin-top: 16px; | ||
+ | } | ||
+ | /* Header | ||
+ | ==================================== */ | ||
+ | .topbar{ | ||
+ | background-color: #e91e63; | ||
+ | padding: 0; | ||
+ | /* color:#fff; */ | ||
+ | } | ||
+ | .topbar .container .row { | ||
+ | margin: 0; | ||
+ | padding:0; | ||
+ | } | ||
+ | .topbar .container .row .col-md-12 { | ||
+ | padding:0; | ||
+ | } | ||
+ | .topbar p{ | ||
+ | margin:0; | ||
+ | display:inline-block; | ||
+ | font-size: 13px; | ||
+ | color: #f1f6ff; | ||
+ | } | ||
+ | .topbar p > i{ | ||
+ | margin-right:5px; | ||
+ | } | ||
+ | .topbar p:last-child{ | ||
+ | text-align:right; | ||
+ | } | ||
+ | header .navbar { | ||
+ | margin-bottom: 0; | ||
+ | } | ||
+ | .topbar li.topbar { | ||
+ | display: inline; | ||
+ | padding-right: 18px; | ||
+ | line-height: 52px; | ||
+ | transition: all .3s linear; | ||
+ | } | ||
− | + | .topbar li.topbar:hover { | |
− | + | color: #1bbde8; | |
− | + | } | |
− | + | ||
− | + | .topbar ul.info i { | |
− | + | color: #131313; | |
− | + | font-size: 16px; | |
− | + | font-style: normal; | |
− | + | margin-right: 8px; | |
− | + | display: inline-block; | |
− | + | position: relative; | |
− | + | top: 4px; | |
+ | } | ||
+ | .topbar ul.info span { | ||
+ | /* line-height: 42px; */ | ||
+ | /* display: inline-block; */ | ||
+ | } | ||
+ | .topbar ul.info li { | ||
+ | float: right; | ||
+ | padding-left: 30px; | ||
+ | color: #ffffff; | ||
+ | font-size: 13px; | ||
+ | line-height: 44px; | ||
+ | } | ||
+ | .topbar ul.info i span { | ||
+ | color: #aaa; | ||
+ | font-size: 13px; | ||
+ | font-family: "Rpboto", sans-serif; | ||
+ | font-weight: 400; | ||
+ | line-height: 50px; | ||
+ | padding-left: 18px; | ||
+ | } | ||
+ | .navbar-default { | ||
+ | border: none; | ||
+ | } | ||
− | + | .navbar-brand { | |
− | + | color: #222; | |
+ | text-transform: uppercase; | ||
+ | font-size: 24px; | ||
+ | font-weight: 700; | ||
+ | line-height: 1em; | ||
+ | letter-spacing: -1px; | ||
+ | margin-top: 21px; | ||
+ | padding: 0 0 0 15px; | ||
+ | } | ||
+ | .navbar-default .navbar-brand{color: #e91e63;font-size: 24px;text-transform: uppercase;} | ||
+ | .navbar-default .navbar-brand:hover { | ||
+ | color: #fff; | ||
+ | } | ||
+ | header .navbar-collapse ul.navbar-nav { | ||
+ | float: right; | ||
+ | margin-right: 0; | ||
+ | } | ||
+ | header .navbar {min-height: 70px;padding: 18px 0;background: #1b1b1b;} | ||
+ | .home-page header .navbar-default{ | ||
+ | background: #1b1b1b; | ||
+ | /* position: absolute; */ | ||
+ | width: 100%; | ||
+ | } | ||
− | + | header .nav li a:hover, | |
− | + | header .nav li a:focus, | |
− | + | header .nav li.active a, | |
+ | header .nav li.active a:hover, | ||
+ | header .nav li a.dropdown-toggle:hover, | ||
+ | header .nav li a.dropdown-toggle:focus, | ||
+ | header .nav li.active ul.dropdown-menu li a:hover, | ||
+ | header .nav li.active ul.dropdown-menu li.active a{ | ||
+ | -webkit-transition: all .3s ease; | ||
+ | -moz-transition: all .3s ease; | ||
+ | -ms-transition: all .3s ease; | ||
+ | -o-transition: all .3s ease; | ||
+ | transition: all .3s ease; | ||
+ | } | ||
− | |||
− | + | header .navbar-default .navbar-nav > .open > a, | |
− | + | header .navbar-default .navbar-nav > .open > a:hover, | |
− | + | header .navbar-default .navbar-nav > .open > a:focus { | |
+ | -webkit-transition: all .3s ease; | ||
+ | -moz-transition: all .3s ease; | ||
+ | -ms-transition: all .3s ease; | ||
+ | -o-transition: all .3s ease; | ||
+ | transition: all .3s ease; | ||
+ | } | ||
− | |||
+ | header .navbar { | ||
+ | min-height: 62px; | ||
+ | padding: 0; | ||
+ | } | ||
+ | header .navbar-nav > li { | ||
+ | padding-bottom: 12px; | ||
+ | padding-top: 12px; | ||
+ | padding: 0 !important; | ||
+ | } | ||
+ | header .navbar-nav > li > a { | ||
+ | /* padding-bottom: 6px; */ | ||
+ | /* padding-top: 5px; */ | ||
+ | margin-left: 2px; | ||
+ | line-height: 40px; | ||
+ | font-weight: 700; | ||
+ | -webkit-transition: all .3s ease; | ||
+ | -moz-transition: all .3s ease; | ||
+ | -ms-transition: all .3s ease; | ||
+ | -o-transition: all .3s ease; | ||
+ | transition: all .3s ease; | ||
+ | } | ||
+ | section.dishes h2 { | ||
+ | color: #fff; | ||
+ | } | ||
+ | .services{ | ||
+ | |||
+ | } | ||
+ | .dishes{ | ||
+ | |||
+ | background: #E91E63; | ||
+ | |||
+ | padding: 35px 0; | ||
+ | |||
+ | color: #ffffff; | ||
+ | } | ||
+ | .dropdown-menu li a:hover { | ||
+ | color: #fff !important; | ||
+ | background: #e91e63 !important; | ||
+ | } | ||
+ | header .nav .caret { | ||
+ | border-bottom-color: #e91e63; | ||
+ | border-top-color: #e91e63; | ||
+ | } | ||
+ | .navbar-default .navbar-nav > .active > a, | ||
+ | .navbar-default .navbar-nav > .active > a:hover, | ||
+ | .navbar-default .navbar-nav > .active > a:focus { | ||
+ | background-color: #fff; | ||
+ | } | ||
+ | .navbar-default .navbar-nav > .open > a, | ||
+ | .navbar-default .navbar-nav > .open > a:hover, | ||
+ | .navbar-default .navbar-nav > .open > a:focus { | ||
+ | background-color: #e91e63; | ||
+ | color: #fff; | ||
+ | } | ||
+ | |||
+ | .dropdown-menu { | ||
+ | box-shadow: none; | ||
+ | border-radius: 0; | ||
+ | border: none; | ||
+ | } | ||
+ | .dropdown-menu li:last-child { | ||
+ | padding-bottom: 0 !important; | ||
+ | margin-bottom: 0; | ||
+ | } | ||
+ | |||
+ | header .nav li .dropdown-menu { | ||
+ | padding: 0; | ||
+ | } | ||
+ | |||
+ | header .nav li .dropdown-menu li a { | ||
+ | line-height: 28px; | ||
+ | padding: 3px 12px; | ||
+ | } | ||
+ | |||
+ | /* --- menu --- */ | ||
+ | .flex-caption p { | ||
+ | line-height: 22px; | ||
+ | font-weight: 300; | ||
+ | color: #ffffff; | ||
+ | text-transform: capitalize; | ||
+ | } | ||
+ | header .navigation { | ||
+ | float:right; | ||
+ | } | ||
+ | |||
+ | header ul.nav li { | ||
+ | border:none; | ||
+ | margin:0; | ||
+ | } | ||
+ | |||
+ | header ul.nav li a { | ||
+ | font-size: 13px; | ||
+ | border:none; | ||
+ | font-weight: 600; | ||
+ | text-transform:uppercase; | ||
+ | } | ||
+ | |||
+ | header ul.nav li ul li a { | ||
+ | font-size:12px; | ||
+ | border:none; | ||
+ | font-weight:300; | ||
+ | text-transform:uppercase; | ||
+ | } | ||
+ | |||
+ | |||
+ | .navbar .nav > li > a { | ||
+ | color: #9a9a9a; | ||
+ | text-shadow: none; | ||
+ | border: 1px solid rgba(255, 255, 255, 0) !important; | ||
+ | } | ||
+ | |||
+ | .navbar .nav a:hover { | ||
+ | background:none; | ||
+ | color: #2e8eda; | ||
+ | } | ||
+ | |||
+ | .navbar .nav > .active > a,.navbar .nav > .active > a:hover { | ||
+ | background:none; | ||
+ | font-weight:700; | ||
+ | } | ||
+ | |||
+ | .navbar .nav > .active > a:active,.navbar .nav > .active > a:focus { | ||
+ | background:none; | ||
+ | outline:0; | ||
+ | font-weight:700; | ||
+ | } | ||
+ | |||
+ | .navbar .nav li .dropdown-menu { | ||
+ | z-index:2000; | ||
+ | } | ||
+ | |||
+ | header ul.nav li ul { | ||
+ | margin-top:1px; | ||
+ | } | ||
+ | header ul.nav li ul li ul { | ||
+ | margin:1px 0 0 1px; | ||
+ | } | ||
+ | .dropdown-menu .dropdown i { | ||
+ | position:absolute; | ||
+ | right:0; | ||
+ | margin-top:3px; | ||
+ | padding-left:20px; | ||
+ | } | ||
+ | |||
+ | .navbar .nav > li > .dropdown-menu:before { | ||
+ | display: inline-block; | ||
+ | border-right: none; | ||
+ | border-bottom: none; | ||
+ | border-left: none; | ||
+ | border-bottom-color: none; | ||
+ | content:none; | ||
+ | } | ||
+ | .navbar-default .navbar-nav>.active>a, .navbar-default .navbar-nav>.active>a:hover, .navbar-default .navbar-nav>.active>a:focus {color: #ffffff;background: #10cdff;border-radius: 0;} | ||
+ | .navbar-default .navbar-nav>li>a:hover, .navbar-default .navbar-nav>li>a:focus { | ||
+ | color: #ffffff; | ||
+ | background-color: transparent; | ||
+ | } | ||
+ | |||
+ | ul.nav li.dropdown a { | ||
+ | z-index:1000; | ||
+ | display:block; | ||
+ | } | ||
+ | |||
+ | select.selectmenu { | ||
+ | display:none; | ||
+ | } | ||
+ | .pageTitle{color: #fff;margin: 30px 0 3px;display: inline-block;font-size: 28px;font-weight: 700;text-transform: uppercase;} | ||
+ | |||
+ | #banner{ | ||
+ | width: 100%; | ||
+ | background:#000; | ||
+ | position:relative; | ||
+ | margin:0; | ||
+ | padding:0; | ||
+ | } | ||
+ | |||
+ | /* Sliders | ||
+ | ==================================== */ | ||
+ | /* --- flexslider --- */ | ||
+ | #main-slider:before {content: '';width: 100%;height: 100%;background: rgba(0, 0, 0, 0.65);z-index: 1;position: absolute;background: rgba(30, 30, 30, 0.31);} | ||
+ | .flex-direction-nav a{ | ||
+ | display:none; | ||
+ | } | ||
+ | .flexslider { | ||
+ | padding:0; | ||
+ | background: #fff; | ||
+ | position: relative; | ||
+ | zoom: 1; | ||
+ | } | ||
+ | .flex-direction-nav .flex-prev{ | ||
+ | left:0px; | ||
+ | } | ||
+ | .flex-direction-nav .flex-next{ | ||
+ | right:0px; | ||
+ | } | ||
+ | .flex-caption {zoom: 1;bottom: 198px;background-color: transparent;color: #fff;margin: 0;padding: 2px 25px 0px 30px;/* position: absolute; */left: 0;text-align: center;margin: 0 auto;right: 0px;display: inline-block;bottom: 10%;width: 50%;right: 0 !important;border-radius: 15px 15px 0 0;} | ||
+ | .flex-caption h3 {color: #fff;letter-spacing: 0px;margin-bottom: 8px;text-transform: uppercase;font-size: 70px;font-weight: 700;} | ||
+ | .flex-caption p {margin: 12px 0 18px;font-size: 20px;font-weight: 400;} | ||
+ | .skill-home{ | ||
+ | margin-bottom:50px; | ||
+ | } | ||
+ | .c1{ | ||
+ | border: #ed5441 1px solid; | ||
+ | background:#ed5441; | ||
+ | } | ||
+ | .c2{ | ||
+ | border: #24c4db 1px solid; | ||
+ | background:#24c4db; | ||
+ | } | ||
+ | .c3{ | ||
+ | border: #1891EC 1px solid; | ||
+ | background:#1891EC; | ||
+ | } | ||
+ | .c4{ | ||
+ | border: #609cec 1px solid; | ||
+ | background:#609cec; | ||
+ | } | ||
+ | .skill-home .icons {padding: 38px;width: 94px;height: 94px;color: #fff;font-size: 42px;font-size: 38px;text-align: center;-ms-border-radius: 50%;-moz-border-radius: 50%;-webkit-border-radius: 50%;border-radius: 0;display: inline-table;border: 4px solid #efefef;color: #ff5722;background: transparent;border-radius: 50%;background: #ffffff;} | ||
+ | .skill-home h2 { | ||
+ | padding-top: 20px; | ||
+ | font-size: 36px; | ||
+ | font-weight: 700; | ||
+ | } | ||
+ | .testimonial-solid { | ||
+ | padding: 50px 0 60px 0; | ||
+ | margin: 0 0 0 0; | ||
+ | background: #FFFFFF; | ||
+ | text-align: center; | ||
+ | } | ||
+ | .testi-icon-area { | ||
+ | text-align: center; | ||
+ | position: absolute; | ||
+ | top: -84px; | ||
+ | margin: 0 auto; | ||
+ | width: 100%; | ||
+ | } | ||
+ | .testi-icon-area .quote { | ||
+ | padding: 15px 0 0 0; | ||
+ | margin: 0 0 0 0; | ||
+ | background: #ffffff; | ||
+ | text-align: center; | ||
+ | color: #1891EC; | ||
+ | display: inline-table; | ||
+ | width: 70px; | ||
+ | height: 70px; | ||
+ | -ms-border-radius: 50%; | ||
+ | -moz-border-radius: 50%; | ||
+ | -webkit-border-radius: 50%; | ||
+ | border-radius: 50%; | ||
+ | font-size: 42px; | ||
+ | border: 1px solid #1891EC; | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | .testi-icon-area .carousel-inner { | ||
+ | margin: 20px 0; | ||
+ | } | ||
+ | .carousel-indicators { | ||
+ | bottom: -30px; | ||
+ | } | ||
+ | .text-center img { | ||
+ | margin: auto; | ||
+ | } | ||
+ | .aboutUs{ | ||
+ | padding:40px 0; | ||
+ | background: #F2F2F2; | ||
+ | } | ||
+ | img.img-center { | ||
+ | margin: 0 auto; | ||
+ | display: block; | ||
+ | max-width: 100%; | ||
+ | } | ||
+ | .home-about{ | ||
+ | padding: 60px 0 0px; | ||
+ | } | ||
+ | .home-about hr{ | ||
+ | padding:0; | ||
+ | margin:0; | ||
+ | } | ||
+ | .home-about .info-box{ | ||
+ | |||
+ | } | ||
+ | .projects { | ||
+ | padding: 60px 0; | ||
+ | background: #1b1b1b; | ||
+ | color: #a7a7a7; | ||
+ | border-top: 4px solid #e91e63; | ||
+ | } | ||
+ | |||
+ | .card-content .price { | ||
+ | font-size: 14px; | ||
+ | color: #e91e63; | ||
+ | } | ||
+ | |||
+ | .projects h4 { | ||
+ | font-size: 16px; | ||
+ | margin: 8px 0; | ||
+ | } | ||
+ | |||
+ | .projects h5 { | ||
+ | font-weight: bold; | ||
+ | font-size: 14px; | ||
+ | margin: 0; | ||
+ | } | ||
+ | /* Testimonial | ||
+ | ----------------------------------*/ | ||
+ | .testimonial-area { | ||
+ | padding: 0 0 0 0; | ||
+ | margin:0; | ||
+ | background: url(../img/low-poly01.jpg) fixed center center; | ||
+ | background-size: cover; | ||
+ | -webkit-background-size: cover; | ||
+ | -moz-background-size: cover; | ||
+ | -ms-background-size: cover; | ||
+ | } | ||
+ | .testimonial-solid p { | ||
+ | color: #000000; | ||
+ | font-size: 16px; | ||
+ | line-height: 30px; | ||
+ | font-style: italic; | ||
+ | } | ||
+ | section.hero-text { | ||
+ | background: #F4783B; | ||
+ | padding:50px 0 50px 0; | ||
+ | color: #fff; | ||
+ | } | ||
+ | section.hero-text h1{ | ||
+ | color: #fff; | ||
+ | } | ||
+ | |||
+ | /* Clients | ||
+ | ------------------------------------ */ | ||
+ | #clients { | ||
+ | padding: 60px 0; | ||
+ | } | ||
+ | #clients .client .img { | ||
+ | height: 76px; | ||
+ | width: 138px; | ||
+ | cursor: pointer; | ||
+ | -webkit-transition: box-shadow .1s linear; | ||
+ | -moz-transition: box-shadow .1s linear; | ||
+ | transition: box-shadow .1s linear; } | ||
+ | #clients .client .img:hover { | ||
+ | cursor: pointer; | ||
+ | /*box-shadow: 0px 0px 2px 0px rgb(155, 155, 155);*/ | ||
+ | border-radius: 8px; } | ||
+ | #clients .client .client1 { | ||
+ | background: url("../img/client1.png") 0 -75px; } | ||
+ | #clients .client .client1:hover { | ||
+ | background-position: 1px 0px; } | ||
+ | #clients .client .client2 { | ||
+ | background: url("../img/client2.png") 0 -75px; } | ||
+ | #clients .client .client2:hover { | ||
+ | background-position: -1px 0px; } | ||
+ | #clients .client .client3 { | ||
+ | background: url("../img/client3.png") 0 -76px; } | ||
+ | #clients .client .client3:hover { | ||
+ | background-position: 0px 0px; } | ||
+ | |||
+ | |||
+ | /* Content | ||
+ | ==================================== */ | ||
+ | |||
+ | #content { | ||
+ | position:relative; | ||
+ | background: #ffffff; | ||
+ | padding:50px 0 0px 0; | ||
+ | } | ||
+ | |||
+ | .cta-text { | ||
+ | text-align: center; | ||
+ | margin-top:10px; | ||
+ | } | ||
+ | |||
+ | |||
+ | .big-cta .cta { | ||
+ | margin-top:10px; | ||
+ | } | ||
+ | |||
+ | .box { | ||
+ | width: 100%; | ||
+ | } | ||
+ | .box-gray { | ||
+ | background: #f8f8f8; | ||
+ | padding: 20px 20px 30px; | ||
+ | } | ||
+ | .box-gray h4,.box-gray i { | ||
+ | margin-bottom: 20px; | ||
+ | } | ||
+ | .box-bottom { | ||
+ | padding: 20px 0; | ||
+ | text-align: center; | ||
+ | } | ||
+ | .box-bottom a { | ||
+ | color: #fff; | ||
+ | font-weight: 700; | ||
+ | } | ||
+ | .box-bottom a:hover { | ||
+ | color: #eee; | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | |||
+ | |||
+ | /* Bottom | ||
+ | ==================================== */ | ||
+ | |||
+ | #bottom { | ||
+ | background:#fcfcfc; | ||
+ | padding:50px 0 0; | ||
+ | |||
+ | } | ||
+ | /* twitter */ | ||
+ | #twitter-wrapper { | ||
+ | text-align: center; | ||
+ | width: 70%; | ||
+ | margin: 0 auto; | ||
+ | } | ||
+ | #twitter em { | ||
+ | font-style: normal; | ||
+ | font-size: 13px; | ||
+ | } | ||
+ | |||
+ | #twitter em.twitterTime a { | ||
+ | font-weight:600; | ||
+ | } | ||
+ | |||
+ | #twitter ul { | ||
+ | padding: 0; | ||
+ | list-style:none; | ||
+ | } | ||
+ | #twitter ul li { | ||
+ | font-size: 20px; | ||
+ | line-height: 1.6em; | ||
+ | font-weight: 300; | ||
+ | margin-bottom: 20px; | ||
+ | position: relative; | ||
+ | word-break: break-word; | ||
+ | } | ||
+ | |||
+ | |||
+ | /* page headline | ||
+ | ==================================== */ | ||
+ | |||
+ | #inner-headline{background: #e4e4e4;position: relative;margin: 0;padding: 0;color: #1891EC;} | ||
+ | |||
+ | |||
+ | #inner-headline h2.pageTitle{ | ||
+ | color: #e91e63; | ||
+ | padding: 5px 0; | ||
+ | display:block; | ||
+ | text-align: center; | ||
+ | } | ||
+ | |||
+ | /* --- breadcrumbs --- */ | ||
+ | #inner-headline ul.breadcrumb { | ||
+ | margin:40px 0; | ||
+ | float:left; | ||
+ | } | ||
+ | |||
+ | #inner-headline ul.breadcrumb li { | ||
+ | margin-bottom:0; | ||
+ | padding-bottom:0; | ||
+ | } | ||
+ | #inner-headline ul.breadcrumb li { | ||
+ | font-size:13px; | ||
+ | color:#fff; | ||
+ | } | ||
+ | |||
+ | #inner-headline ul.breadcrumb li i{ | ||
+ | color:#dedede; | ||
+ | } | ||
+ | |||
+ | #inner-headline ul.breadcrumb li a { | ||
+ | color:#fff; | ||
+ | } | ||
+ | |||
+ | ul.breadcrumb li a:hover { | ||
+ | text-decoration:none; | ||
+ | } | ||
+ | |||
+ | /* Forms | ||
+ | ============================= */ | ||
+ | |||
+ | /* --- contact form ---- */ | ||
+ | form#contactform input[type="text"] { | ||
+ | width: 100%; | ||
+ | border: 1px solid #f5f5f5; | ||
+ | min-height: 40px; | ||
+ | padding-left:20px; | ||
+ | font-size:13px; | ||
+ | padding-right:20px; | ||
+ | -webkit-box-sizing: border-box; | ||
+ | -moz-box-sizing: border-box; | ||
+ | box-sizing: border-box; | ||
+ | |||
+ | } | ||
+ | |||
+ | form#contactform textarea { | ||
+ | border: 1px solid #f5f5f5; | ||
+ | width: 100%; | ||
+ | padding-left:20px; | ||
+ | padding-top:10px; | ||
+ | font-size:13px; | ||
+ | padding-right:20px; | ||
+ | -webkit-box-sizing: border-box; | ||
+ | -moz-box-sizing: border-box; | ||
+ | box-sizing: border-box; | ||
+ | |||
+ | } | ||
+ | |||
+ | form#contactform .validation { | ||
+ | font-size:11px; | ||
+ | } | ||
+ | |||
+ | #sendmessage { | ||
+ | border:1px solid #e6e6e6; | ||
+ | background:#f6f6f6; | ||
+ | display:none; | ||
+ | text-align:center; | ||
+ | padding:15px 12px 15px 65px; | ||
+ | margin:10px 0; | ||
+ | font-weight:600; | ||
+ | margin-bottom:30px; | ||
+ | |||
+ | } | ||
+ | |||
+ | #sendmessage.show,.show { | ||
+ | display:block; | ||
+ | } | ||
+ | |||
+ | form#commentform input[type="text"] { | ||
+ | width: 100%; | ||
+ | min-height: 40px; | ||
+ | padding-left:20px; | ||
+ | font-size:13px; | ||
+ | padding-right:20px; | ||
+ | -webkit-box-sizing: border-box; | ||
+ | -moz-box-sizing: border-box; | ||
+ | box-sizing: border-box; | ||
+ | -webkit-border-radius: 2px 2px 2px 2px; | ||
+ | -moz-border-radius: 2px 2px 2px 2px; | ||
+ | border-radius: 2px 2px 2px 2px; | ||
+ | |||
+ | } | ||
+ | |||
+ | form#commentform textarea { | ||
+ | width: 100%; | ||
+ | padding-left:20px; | ||
+ | padding-top:10px; | ||
+ | font-size:13px; | ||
+ | padding-right:20px; | ||
+ | -webkit-box-sizing: border-box; | ||
+ | -moz-box-sizing: border-box; | ||
+ | box-sizing: border-box; | ||
+ | -webkit-border-radius: 2px 2px 2px 2px; | ||
+ | -moz-border-radius: 2px 2px 2px 2px; | ||
+ | border-radius: 2px 2px 2px 2px; | ||
+ | } | ||
+ | |||
+ | |||
+ | /* --- search form --- */ | ||
+ | .search{ | ||
+ | float:right; | ||
+ | margin:35px 0 0; | ||
+ | padding-bottom:0; | ||
+ | } | ||
+ | |||
+ | #inner-headline form.input-append { | ||
+ | margin:0; | ||
+ | padding:0; | ||
+ | } | ||
+ | |||
+ | .about-text h3 { | ||
+ | font-size: 18px; | ||
+ | padding-bottom: 0; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | |||
+ | .about-text .btn { | ||
+ | margin-top: 20px; | ||
+ | //font-size: 15px; | ||
+ | //text-align: justify; | ||
+ | } | ||
+ | |||
+ | .about-image img { | ||
+ | } | ||
+ | |||
+ | .about-text ul { | ||
+ | margin-top: 30px; | ||
+ | padding: 0; | ||
+ | } | ||
+ | |||
+ | .about-text li { | ||
+ | margin-bottom: 10px; | ||
+ | // | ||
+ | font-weight: bold; | ||
+ | list-style: none; | ||
+ | } | ||
+ | |||
+ | |||
+ | .about-text-2 img { | ||
+ | border: 5px solid #ddd; | ||
+ | margin-bottom: 20px; | ||
+ | } | ||
+ | |||
+ | .about-text-2 p { | ||
+ | margin-bottom: 20px; | ||
+ | } | ||
+ | |||
+ | #call-to-action { | ||
+ | background: url(../images/bg/about.jpg) no-repeat; | ||
+ | background-attachment: fixed; | ||
+ | background-position: top center; | ||
+ | padding: 100px 0; | ||
+ | color: #fff; | ||
+ | } | ||
+ | |||
+ | .call-to-action h3 { | ||
+ | font-size: 40px; | ||
+ | letter-spacing: 15px; | ||
+ | color: #fff; | ||
+ | padding-bottom: 30px; | ||
+ | } | ||
+ | |||
+ | .call-to-action p { | ||
+ | color: #999; | ||
+ | padding-bottom: 20px; | ||
+ | } | ||
+ | |||
+ | .call-to-action .btn-primary { | ||
+ | background-color: transparent; | ||
+ | border-color: #999; | ||
+ | } | ||
+ | |||
+ | .call-to-action .btn-primary:hover { | ||
+ | background-color: #151515; | ||
+ | color: #999; | ||
+ | border-color: #151515; | ||
+ | } | ||
+ | |||
+ | #call-to-action-2 { | ||
+ | background-color: #efefef; | ||
+ | padding: 50px 0; | ||
+ | } | ||
+ | #call-to-action-2 h3 { | ||
+ | color: #05142b; | ||
+ | font-size: 24px; | ||
+ | font-weight: 600; | ||
+ | text-transform: uppercase; | ||
+ | } | ||
+ | #call-to-action-2 p { | ||
+ | color: #5f5f5f; | ||
+ | font-size: 15px; | ||
+ | } | ||
+ | #call-to-action-2 .btn-primary { | ||
+ | border-color: #FFFFFF; | ||
+ | /* background: transparent; */ | ||
+ | margin-top: 50px; | ||
+ | color: #fff; | ||
+ | } | ||
+ | section.section-padding.gray-bg { | ||
+ | background: #ffffff; | ||
+ | padding: 60px; | ||
+ | color: #484848; | ||
+ | } | ||
+ | |||
+ | #accordion-alt3 .panel-heading h4 { | ||
+ | font-size: 13px; | ||
+ | line-height: 28px; | ||
+ | } | ||
+ | .panel .panel-heading h4 { | ||
+ | font-weight: 400; | ||
+ | } | ||
+ | .panel-title { | ||
+ | margin-top: 0; | ||
+ | margin-bottom: 0; | ||
+ | font-size: 15px; | ||
+ | color: inherit; | ||
+ | } | ||
+ | .panel-group .panel { | ||
+ | margin-bottom: 0; | ||
+ | border-radius: 2px; | ||
+ | } | ||
+ | .panel {margin-bottom: 18px;background-color: #FFFFFF;border: 1px solid #f3f3f3;border-radius: 2px;-webkit-box-shadow: 0 1px 1px rgba(0,0,0,0.05);box-shadow: 0 1px 1px rgba(0,0,0,0.05);} | ||
+ | #accordion-alt3 .panel-heading h4 a i {font-size: 17px;line-height: 18px;width: 18px;height: 18px;margin-right: 8px;color: #A0A0A0;text-align: center;border-radius: 50%;margin-left: 6px;font-weight: bold;} | ||
+ | .progress.pb-sm { | ||
+ | height: 6px!important; | ||
+ | } | ||
+ | .progress { | ||
+ | box-shadow: inset 0 0 2px rgba(0,0,0,.1); | ||
+ | } | ||
+ | .progress { | ||
+ | overflow: hidden; | ||
+ | height: 18px; | ||
+ | margin-bottom: 18px; | ||
+ | background-color: #f5f5f5; | ||
+ | border-radius: 2px; | ||
+ | -webkit-box-shadow: inset 0 1px 2px rgba(0,0,0,0.1); | ||
+ | box-shadow: inset 0 1px 2px rgba(0,0,0,0.1); | ||
+ | } | ||
+ | .progress .progress-bar.progress-bar-red { | ||
+ | background: #ed5441; | ||
+ | } | ||
+ | .progress .progress-bar.progress-bar-green { | ||
+ | background: #51d466; | ||
+ | } | ||
+ | .progress .progress-bar.progress-bar-lblue { | ||
+ | background: #32c8de; | ||
+ | } | ||
+ | /* --- portfolio detail --- */ | ||
+ | .top-wrapper { | ||
+ | margin-bottom:20px; | ||
+ | } | ||
+ | .info-blocks {margin-bottom: 15px;/* background: #efefef; */} | ||
+ | .info-blocks i.icon-info-blocks {float: left;color: #e91e63;font-size: 40px;min-width: 50px;margin-top: 11px;text-align: center;background: #f5f5f5;width: 72px;height: 72px;/* padding-top: 21px; *//* border: 1px solid #e0e0e0; */line-height: 75px;} | ||
+ | .info-blocks .info-blocks-in {padding: 15px;overflow: hidden;background: #f5f5f5;} | ||
+ | .info-blocks .info-blocks-in h3 {color: #202020;font-size: 20px;line-height: 28px;margin:0px;margin-bottom: 8px;/* font-weight: bold; */} | ||
+ | .info-blocks .info-blocks-in p {font-size: 14px;} | ||
+ | |||
+ | blockquote { | ||
+ | font-size:16px; | ||
+ | font-weight:400; | ||
+ | font-family:'Noto Serif', serif; | ||
+ | font-style:italic; | ||
+ | padding-left:0; | ||
+ | color:#a2a2a2; | ||
+ | line-height:1.6em; | ||
+ | border:none; | ||
+ | } | ||
+ | |||
+ | blockquote cite { display:block; font-size:12px; color:#666; margin-top:10px; } | ||
+ | blockquote cite:before { content:"\2014 \0020"; } | ||
+ | blockquote cite a, | ||
+ | blockquote cite a:visited, | ||
+ | blockquote cite a:visited { color:#555; } | ||
+ | |||
+ | /* --- pullquotes --- */ | ||
+ | |||
+ | .pullquote-left { | ||
+ | display:block; | ||
+ | color:#a2a2a2; | ||
+ | font-family:'Noto Serif', serif; | ||
+ | font-size:14px; | ||
+ | line-height:1.6em; | ||
+ | padding-left:20px; | ||
+ | } | ||
+ | |||
+ | .pullquote-right { | ||
+ | display:block; | ||
+ | color:#a2a2a2; | ||
+ | font-family:'Noto Serif', serif; | ||
+ | font-size:14px; | ||
+ | line-height:1.6em; | ||
+ | padding-right:20px; | ||
+ | } | ||
+ | |||
+ | /* --- button --- */ | ||
+ | .btn{text-align: center;color: #FFFFFF;background: #e91e63;border-color: #e91e63;width: 120px;border-radius: 0;} | ||
+ | .btn-theme { | ||
+ | color: #fff; | ||
+ | background: transparent; | ||
+ | border: 1px solid #fff; | ||
+ | padding: 12px 30px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | a.btn.btn-details { | ||
+ | float: right; | ||
+ | margin-top: -34px; | ||
+ | font-size: 14px; | ||
+ | height: 34px; | ||
+ | width: 96px; | ||
+ | text-transform: capitalize; | ||
+ | } | ||
+ | .btn-theme:hover { | ||
+ | color: #eee; | ||
+ | } | ||
+ | |||
+ | /* --- list style --- */ | ||
+ | |||
+ | ul.general { | ||
+ | list-style:none; | ||
+ | margin-left:0; | ||
+ | } | ||
+ | |||
+ | ul.link-list{ | ||
+ | margin:0; | ||
+ | padding:0; | ||
+ | list-style:none; | ||
+ | } | ||
+ | |||
+ | ul.link-list li{ | ||
+ | margin:0; | ||
+ | padding:2px 0 2px 0; | ||
+ | list-style:none; | ||
+ | } | ||
+ | footer{background: #0e0e0e;} | ||
+ | footer ul.link-list li a{ | ||
+ | color: #8C8C8C; | ||
+ | } | ||
+ | footer ul.link-list li a:hover { | ||
+ | color: #e91e63; | ||
+ | } | ||
+ | /* --- Heading style --- */ | ||
+ | |||
+ | h4.heading { | ||
+ | font-weight:700; | ||
+ | } | ||
+ | |||
+ | .heading { margin-bottom: 30px; } | ||
+ | |||
+ | .heading { | ||
+ | position: relative; | ||
+ | |||
+ | } | ||
+ | |||
+ | |||
+ | .widgetheading { | ||
+ | width:100%; | ||
+ | padding:0; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | |||
+ | #bottom .widgetheading { | ||
+ | position: relative; | ||
+ | border-bottom: #e6e6e6 1px solid; | ||
+ | padding-bottom: 9px; | ||
+ | } | ||
+ | |||
+ | aside .widgetheading { | ||
+ | position: relative; | ||
+ | border-bottom: #e9e9e9 1px solid; | ||
+ | padding-bottom: 9px; | ||
+ | } | ||
+ | |||
+ | footer .widgetheading { | ||
+ | position: relative; | ||
+ | } | ||
+ | |||
+ | footer .widget .social-network { | ||
+ | position:relative; | ||
+ | } | ||
+ | |||
+ | .team-member h4{ | ||
+ | text-align: center; | ||
+ | margin-bottom: 5px; | ||
+ | } | ||
+ | .team-member .deg{ | ||
+ | text-align:center; | ||
+ | display:block; | ||
+ | } | ||
+ | #bottom .widget .widgetheading span, aside .widget .widgetheading span, footer .widget .widgetheading span { | ||
+ | position: absolute; | ||
+ | width: 60px; | ||
+ | height: 1px; | ||
+ | bottom: -1px; | ||
+ | right:0; | ||
+ | |||
+ | } | ||
+ | .panel-title>a { | ||
+ | color: inherit; | ||
+ | text-decoration: none; | ||
+ | font-weight: 600; | ||
+ | } | ||
+ | .box-area{padding: 15px;background: #ffffff;margin-top: -21px;border: 2px solid #f3f3f3;} | ||
+ | .box-area h3{font-size: 17px;font-weight: 600;} | ||
+ | /* --- Map --- */ | ||
+ | .map{ | ||
+ | position:relative; | ||
+ | margin-top:-50px; | ||
+ | margin-bottom:40px; | ||
+ | } | ||
+ | |||
+ | .map iframe{ | ||
+ | width:100%; | ||
+ | height:450px; | ||
+ | border:none; | ||
+ | } | ||
+ | |||
+ | .map-grid iframe{ | ||
+ | width:100%; | ||
+ | height:350px; | ||
+ | border:none; | ||
+ | margin:0 0 -5px 0; | ||
+ | padding:0; | ||
+ | } | ||
+ | |||
+ | |||
+ | ul.team-detail{ | ||
+ | margin:-10px 0 0 0; | ||
+ | padding:0; | ||
+ | list-style:none; | ||
+ | } | ||
+ | |||
+ | ul.team-detail li{ | ||
+ | border-bottom:1px dotted #e9e9e9; | ||
+ | margin:0 0 15px 0; | ||
+ | padding:0 0 15px 0; | ||
+ | list-style:none; | ||
+ | } | ||
+ | |||
+ | ul.team-detail li label { | ||
+ | font-size:13px; | ||
+ | } | ||
+ | |||
+ | ul.team-detail li h4, ul.team-detail li label{ | ||
+ | margin-bottom:0; | ||
+ | } | ||
+ | |||
+ | ul.team-detail li ul.social-network { | ||
+ | border:none; | ||
+ | margin:0; | ||
+ | padding:0; | ||
+ | } | ||
+ | |||
+ | ul.team-detail li ul.social-network li { | ||
+ | border:none; | ||
+ | margin:0; | ||
+ | } | ||
+ | ul.team-detail li ul.social-network li i { | ||
+ | margin:0; | ||
+ | } | ||
+ | |||
+ | .navbar-brand i { | ||
+ | color: #ffffff; | ||
+ | font-size: 26px; | ||
+ | margin-right: 5px; | ||
+ | position: relative; | ||
+ | top: 3px; | ||
+ | } | ||
+ | .pricing-title{ | ||
+ | background:#fff; | ||
+ | text-align:center; | ||
+ | padding:10px 0 10px 0; | ||
+ | } | ||
+ | |||
+ | .pricing-title h3{ | ||
+ | font-weight:600; | ||
+ | margin-bottom:0; | ||
+ | } | ||
+ | |||
+ | .pricing-offer{ | ||
+ | background: #fcfcfc; | ||
+ | text-align: center; | ||
+ | padding:40px 0 40px 0; | ||
+ | font-size:18px; | ||
+ | border-top:1px solid #e6e6e6; | ||
+ | border-bottom:1px solid #e6e6e6; | ||
+ | } | ||
+ | |||
+ | .pricing-box.activeItem .pricing-offer{ | ||
+ | color:#fff; | ||
+ | } | ||
+ | |||
+ | .pricing-offer strong{ | ||
+ | font-size:78px; | ||
+ | line-height:89px; | ||
+ | } | ||
+ | |||
+ | .pricing-offer sup{ | ||
+ | font-size:28px; | ||
+ | } | ||
+ | |||
+ | .pricing-container{ | ||
+ | background: #fff; | ||
+ | text-align:center; | ||
+ | font-size:14px; | ||
+ | } | ||
+ | |||
+ | .pricing-container strong{ | ||
+ | color:#353535; | ||
+ | } | ||
+ | |||
+ | .pricing-container ul{ | ||
+ | list-style:none; | ||
+ | padding:0; | ||
+ | margin:0; | ||
+ | } | ||
+ | |||
+ | .pricing-container ul li{ | ||
+ | border-bottom: 1px solid #CFCFCF; | ||
+ | list-style: none; | ||
+ | padding: 15px 0 15px 0; | ||
+ | margin: 0 0 0 0; | ||
+ | color: #222; | ||
+ | } | ||
+ | |||
+ | .pricing-action{ | ||
+ | margin:0; | ||
+ | background: #fcfcfc; | ||
+ | text-align:center; | ||
+ | padding:20px 0 30px 0; | ||
+ | } | ||
+ | |||
+ | .pricing-wrapp{ | ||
+ | margin:0 auto; | ||
+ | width:100%; | ||
+ | background:#fd0000; | ||
+ | } | ||
+ | .pricing-box-item {border: 1px solid #e6e6e6;background:#fcfcfc;position:relative;margin:0 0 20px 0;padding:0;-webkit-box-shadow: 0 2px 0 rgba(0,0,0,0.03);-moz-box-shadow: 0 2px 0 rgba(0,0,0,0.03);box-shadow: 0 2px 0 rgba(0,0,0,0.03);-webkit-box-sizing: border-box;-moz-box-sizing: border-box;box-sizing: border-box;overflow: hidden;} | ||
+ | |||
+ | .pricing-box-item .pricing-heading { | ||
+ | background: #ffffff; | ||
+ | text-align: center; | ||
+ | padding: 50px 0; | ||
+ | display:block; | ||
+ | } | ||
+ | .pricing-box-item.activeItem .pricing-heading { | ||
+ | /* background: #f3f3f3; */ | ||
+ | text-align: center; | ||
+ | border-bottom:none; | ||
+ | display:block; | ||
+ | color:#fff; | ||
+ | } | ||
+ | .pricing-box-item.activeItem .pricing-heading h3 { | ||
+ | |||
+ | color: #fff; | ||
+ | } | ||
+ | |||
+ | .pricing-box-item .pricing-heading h3 strong { | ||
+ | font-size:28px; | ||
+ | font-weight:700; | ||
+ | letter-spacing:-1px; | ||
+ | color: #05142b; | ||
+ | } | ||
+ | .pricing-box-item .pricing-heading h3 { | ||
+ | font-size:32px; | ||
+ | font-weight:300; | ||
+ | letter-spacing:-1px; | ||
+ | } | ||
+ | |||
+ | .pricing-box-item .pricing-terms { | ||
+ | text-align: center; | ||
+ | background: #fff; | ||
+ | display: block; | ||
+ | overflow: hidden; | ||
+ | padding: 11px 0 5px; | ||
+ | /* border-top: 2px solid #BBBBBB; */ | ||
+ | border-bottom: 2px dotted #CDCDCD; | ||
+ | } | ||
+ | |||
+ | .pricing-box-item .pricing-terms h6 { | ||
+ | margin-top: 16px; | ||
+ | color: #ff5722; | ||
+ | font-size: 22px; | ||
+ | } | ||
+ | |||
+ | .pricing-box-item .icon .price-circled { | ||
+ | margin: 10px 10px 10px 0; | ||
+ | display: inline-block !important; | ||
+ | text-align: center !important; | ||
+ | color: #fff; | ||
+ | width: 68px; | ||
+ | height: 68px; | ||
+ | padding:12px; | ||
+ | font-size: 16px; | ||
+ | font-weight:700; | ||
+ | line-height: 68px; | ||
+ | text-shadow:none; | ||
+ | cursor: pointer; | ||
+ | background-color: #888; | ||
+ | border-radius: 64px; | ||
+ | -moz-border-radius: 64px; | ||
+ | -webkit-border-radius: 64px; | ||
+ | } | ||
+ | |||
+ | .pricing-box-item .pricing-action{ | ||
+ | margin:0; | ||
+ | text-align:center; | ||
+ | padding:30px 0 30px 0; | ||
+ | } | ||
+ | .pricing-action .btn{ | ||
+ | background: #ff5722; | ||
+ | color: #fff; | ||
+ | } | ||
+ | .menuItem .menu{font-size:20px;font-weight:600;list-style:none;padding:0 0 15px;margin-bottom:20px;display:inline-block;color: #e91e63;} | ||
+ | .menuItem .menu li{padding: 16px 30px 0px;margin-bottom: 0;display:inline-block;width:100%;float:left;background: #ffffff;line-height: 30px;border-left: 2px solid #e91e63;border-radius: 0;} | ||
+ | .menuItem .detail{ | ||
+ | font-size:13px; | ||
+ | color:#444; | ||
+ | font-weight:normal; | ||
+ | } | ||
+ | .menuItem .price{ | ||
+ | font-size:13px; | ||
+ | float:right; | ||
+ | font-weight:600; | ||
+ | } | ||
+ | /* Testimonials */ | ||
+ | .testimonials blockquote { | ||
+ | background: #ffffff none repeat scroll 0 0; | ||
+ | border: medium none; | ||
+ | color: #666; | ||
+ | display: block; | ||
+ | font-size: 13px; | ||
+ | line-height: 20px; | ||
+ | padding: 15px; | ||
+ | position: relative; | ||
+ | } | ||
+ | .testimonials blockquote::before { | ||
+ | width: 0; | ||
+ | height: 0; | ||
+ | right: 0; | ||
+ | bottom: 0; | ||
+ | content: " "; | ||
+ | display: block; | ||
+ | position: absolute; | ||
+ | border-bottom: 20px solid #fff; | ||
+ | border-right: 0 solid transparent; | ||
+ | border-left: 15px solid transparent; | ||
+ | border-left-style: inset; /*FF fixes*/ | ||
+ | border-bottom-style: inset; /*FF fixes*/ | ||
+ | } | ||
+ | .testimonials blockquote::after { | ||
+ | width: 0; | ||
+ | height: 0; | ||
+ | right: 0; | ||
+ | bottom: 0; | ||
+ | content: " "; | ||
+ | display: block; | ||
+ | position: absolute; | ||
+ | border-style: solid; | ||
+ | border-width: 20px 20px 0 0; | ||
+ | border-color: #c5c5c5 transparent transparent transparent; | ||
+ | } | ||
+ | .testimonials .carousel-info img { | ||
+ | border: 1px solid #cccccc; | ||
+ | border-radius: 150px !important; | ||
+ | height: 75px; | ||
+ | padding: 3px; | ||
+ | width: 75px; | ||
+ | } | ||
+ | .testimonials .carousel-info { | ||
+ | overflow: hidden; | ||
+ | } | ||
+ | .testimonials .carousel-info img { | ||
+ | margin-right: 15px; | ||
+ | } | ||
+ | .testimonials .carousel-info span { | ||
+ | display: block; | ||
+ | } | ||
+ | .testimonials span.testimonials-name { | ||
+ | color: #e91e63; | ||
+ | font-size: 16px; | ||
+ | font-weight: 300; | ||
+ | margin: 23px 0 7px; | ||
+ | } | ||
+ | .testimonials span.testimonials-post { | ||
+ | color: #656565; | ||
+ | font-size: 12px; | ||
+ | } | ||
+ | /* ===== Widgets ===== */ | ||
+ | |||
+ | /* --- flickr --- */ | ||
+ | .widget .flickr_badge { | ||
+ | width:100%; | ||
+ | } | ||
+ | .widget .flickr_badge img { margin: 0 9px 20px 0; } | ||
+ | |||
+ | footer .widget .flickr_badge { | ||
+ | width: 100%; | ||
+ | } | ||
+ | footer .widget .flickr_badge img { | ||
+ | margin: 0 9px 20px 0; | ||
+ | } | ||
+ | |||
+ | .flickr_badge img { | ||
+ | width: 50px; | ||
+ | height: 50px; | ||
+ | float: left; | ||
+ | margin: 0 9px 20px 0; | ||
+ | } | ||
+ | |||
+ | /* --- Recent post widget --- */ | ||
+ | |||
+ | .recent-post{ | ||
+ | margin:20px 0 0 0; | ||
+ | padding:0; | ||
+ | line-height:18px; | ||
+ | } | ||
+ | |||
+ | .recent-post h5 a:hover { | ||
+ | text-decoration:none; | ||
+ | } | ||
+ | |||
+ | .recent-post .text h5 a { | ||
+ | color:#353535; | ||
+ | } | ||
+ | |||
+ | |||
+ | footer{ | ||
+ | padding:50px 0 0 0; | ||
+ | color:#f8f8f8; | ||
+ | } | ||
+ | |||
+ | footer a { | ||
+ | color:#fff; | ||
+ | } | ||
+ | |||
+ | footer a:hover { | ||
+ | color: #e91e63; | ||
+ | } | ||
+ | |||
+ | footer h1, footer h2, footer h3, footer h4, footer h5, footer h6{ | ||
+ | color: #d3d3d3; | ||
+ | } | ||
+ | |||
+ | footer address { | ||
+ | line-height:1.6em; | ||
+ | color: #797979; | ||
+ | } | ||
+ | |||
+ | footer h5 a:hover, footer a:hover { | ||
+ | text-decoration:none; | ||
+ | } | ||
+ | |||
+ | ul.social-network { | ||
+ | list-style:none; | ||
+ | margin: 4px 0 0 -25px; | ||
+ | } | ||
+ | |||
+ | ul.social-network li { | ||
+ | display:inline; | ||
+ | margin: 0 5px; | ||
+ | /* border: 1px solid #2D2D2D; */ | ||
+ | padding: 5px 0 0; | ||
+ | width: 32px; | ||
+ | display: inline-block; | ||
+ | text-align: center; | ||
+ | height: 32px; | ||
+ | vertical-align: baseline; | ||
+ | } | ||
+ | |||
+ | #sub-footer{ | ||
+ | text-shadow:none; | ||
+ | color: #dedede; | ||
+ | padding:0; | ||
+ | padding-top:30px; | ||
+ | margin:20px 0 0 0; | ||
+ | background: #0a0a0a; | ||
+ | border-top: 1px solid #3e3e3e; | ||
+ | } | ||
+ | |||
+ | #sub-footer p{ | ||
+ | margin:0; | ||
+ | padding:0; | ||
+ | } | ||
+ | |||
+ | #sub-footer span{ | ||
+ | color: #9e9e9e; | ||
+ | } | ||
+ | |||
+ | .copyright { | ||
+ | text-align:left; | ||
+ | font-size:12px; | ||
+ | } | ||
+ | |||
+ | #sub-footer ul.social-network { | ||
+ | float:right; | ||
+ | } | ||
+ | |||
+ | |||
+ | |||
+ | /* scroll to top */ | ||
+ | .scrollup{ | ||
+ | position:fixed; | ||
+ | width:32px; | ||
+ | height:32px; | ||
+ | bottom:0px; | ||
+ | right:20px; | ||
+ | background: #e91e63; | ||
+ | } | ||
+ | |||
+ | a.scrollup { | ||
+ | outline:0; | ||
+ | text-align: center; | ||
+ | } | ||
+ | |||
+ | a.scrollup:hover,a.scrollup:active,a.scrollup:focus { | ||
+ | opacity:1; | ||
+ | text-decoration:none; | ||
+ | } | ||
+ | a.scrollup i { | ||
+ | margin-top: 10px; | ||
+ | color: #fff; | ||
+ | } | ||
+ | a.scrollup i:hover { | ||
+ | text-decoration:none; | ||
+ | } | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | .absolute{ | ||
+ | position:absolute; | ||
+ | } | ||
+ | |||
+ | .relative{ | ||
+ | position:relative; | ||
+ | } | ||
+ | |||
+ | .aligncenter{ | ||
+ | text-align:center; | ||
+ | } | ||
+ | |||
+ | .aligncenter span{ | ||
+ | margin-left:0; | ||
+ | } | ||
+ | |||
+ | .floatright { | ||
+ | float:right; | ||
+ | } | ||
+ | |||
+ | .floatleft { | ||
+ | float:left; | ||
+ | } | ||
+ | |||
+ | .floatnone { | ||
+ | float:none; | ||
+ | } | ||
+ | |||
+ | .aligncenter { | ||
+ | text-align:center; | ||
+ | } | ||
+ | |||
+ | img.pull-left, .align-left{ | ||
+ | float:left; | ||
+ | margin:0 15px 15px 0; | ||
+ | } | ||
+ | |||
+ | .widget img.pull-left { | ||
+ | float:left; | ||
+ | margin:0 15px 15px 0; | ||
+ | } | ||
+ | |||
+ | img.pull-right, .align-right { | ||
+ | float:right; | ||
+ | margin:0 0 15px 15px; | ||
+ | } | ||
+ | |||
+ | article img.pull-left, article .align-left{ | ||
+ | float:left; | ||
+ | margin:5px 15px 15px 0; | ||
+ | } | ||
+ | |||
+ | article img.pull-right, article .align-right{ | ||
+ | float:right; | ||
+ | margin:5px 0 15px 15px; | ||
+ | } | ||
+ | ============================= */ | ||
+ | |||
+ | .clear-marginbot{ | ||
+ | margin-bottom:0; | ||
+ | } | ||
+ | |||
+ | .marginbot10{ | ||
+ | margin-bottom:10px; | ||
+ | } | ||
+ | .marginbot20{ | ||
+ | margin-bottom:20px; | ||
+ | } | ||
+ | .marginbot30{ | ||
+ | margin-bottom:30px; | ||
+ | } | ||
+ | .marginbot40{ | ||
+ | margin-bottom:40px; | ||
+ | } | ||
+ | |||
+ | .clear-margintop{ | ||
+ | margin-top:0; | ||
+ | } | ||
+ | |||
+ | .margintop10{ | ||
+ | margin-top:10px; | ||
+ | } | ||
+ | |||
+ | .margintop20{ | ||
+ | margin-top:20px; | ||
+ | } | ||
+ | |||
+ | .margintop30{ | ||
+ | margin-top:30px; | ||
+ | } | ||
+ | |||
+ | .margintop40{ | ||
+ | margin-top:40px; | ||
+ | } | ||
+ | .flex-control-paging li a { | ||
+ | width: 14px; | ||
+ | height: 14px; | ||
+ | border-radius: 50%; | ||
+ | background: #ffffff; | ||
+ | text-indent: 50px; | ||
+ | overflow: hidden; | ||
+ | } | ||
+ | .btn:hover, .btn:focus { | ||
+ | color: #FFF; | ||
+ | text-decoration: none; | ||
+ | background: #d41a59; | ||
+ | border-color: #d41a59; | ||
+ | } | ||
+ | /* Media queries | ||
+ | ============================= */ | ||
+ | |||
+ | @media (min-width: 768px) and (max-width: 979px) { | ||
+ | |||
+ | a.detail{ | ||
+ | background:none; | ||
+ | width:100%; | ||
+ | } | ||
+ | |||
+ | header .navbar-nav > li > a{ | ||
+ | |||
+ | } | ||
+ | |||
+ | footer .widget form input#appendedInputButton { | ||
+ | display: block; | ||
+ | width: 91%; | ||
+ | -webkit-border-radius: 4px 4px 4px 4px; | ||
+ | -moz-border-radius: 4px 4px 4px 4px; | ||
+ | border-radius: 4px 4px 4px 4px; | ||
+ | } | ||
+ | |||
+ | footer .widget form .input-append .btn { | ||
+ | display: block; | ||
+ | width: 100%; | ||
+ | padding-right: 0; | ||
+ | padding-left: 0; | ||
+ | -webkit-box-sizing: border-box; | ||
+ | -moz-box-sizing: border-box; | ||
+ | box-sizing: border-box; | ||
+ | margin-top:10px; | ||
+ | } | ||
+ | |||
+ | ul.related-folio li{ | ||
+ | width:156px; | ||
+ | margin:0 20px 0 0; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | @media (max-width: 767px) { | ||
+ | .navbar-default .navbar-collapse{border-color: none;/* background: #05142b; */} | ||
+ | .navbar-default .navbar-toggle .icon-bar {background-color: #868686;border-radius: 0;} | ||
+ | .navbar-default .navbar-toggle:hover, .navbar-default .navbar-toggle:focus { | ||
+ | background-color: rgba(221, 221, 221, 0); | ||
+ | } | ||
+ | .navbar-default .navbar-collapse, .navbar-default .navbar-form { | ||
+ | border-color: rgba(255, 255, 255, 0.58); | ||
+ | margin-top: 15px; | ||
+ | } | ||
+ | .flex-caption h3{ | ||
+ | font-size:28px; | ||
+ | } | ||
+ | body { | ||
+ | padding-right: 0; | ||
+ | padding-left: 0; | ||
+ | } | ||
+ | .navbar-brand { | ||
+ | margin-top: 18px; | ||
+ | border-bottom: none; | ||
+ | } | ||
+ | .navbar-header { | ||
+ | /* margin-top: 20px; */ | ||
+ | border-bottom: none; | ||
+ | } | ||
+ | |||
+ | .navbar-nav { | ||
+ | border-top: none; | ||
+ | float: none; | ||
+ | width: 100%; | ||
+ | } | ||
+ | .navbar .nav > .active > a, .navbar .nav > .active > a:hover {background: none;font-weight: 700;color: #10cdff;} | ||
+ | header .navbar-nav > li {padding-bottom: 0;padding-top: 0;} | ||
+ | header .nav li .dropdown-menu { | ||
+ | margin-top: 0; | ||
+ | } | ||
+ | header .navbar-nav > li > a{ | ||
+ | line-height: 20px; | ||
+ | } | ||
+ | .dropdown-menu { | ||
+ | position: absolute; | ||
+ | top: 0; | ||
+ | left: 40px; | ||
+ | z-index: 1000; | ||
+ | display: none; | ||
+ | float: left; | ||
+ | min-width: 160px; | ||
+ | padding: 5px 0; | ||
+ | margin: 2px 0 0; | ||
+ | font-size: 13px; | ||
+ | list-style: none; | ||
+ | background-color: #fff; | ||
+ | background-clip: padding-box; | ||
+ | border: 1px solid #f5f5f5; | ||
+ | border: 1px solid rgba(0, 0, 0, .15); | ||
+ | border-radius: 0; | ||
+ | -webkit-box-shadow: 0 6px 12px rgba(0, 0, 0, .175); | ||
+ | box-shadow: 0 6px 12px rgba(0, 0, 0, .175); | ||
+ | } | ||
+ | |||
+ | |||
+ | |||
+ | li.active { | ||
+ | border: none; | ||
+ | overflow: hidden; | ||
+ | /* background: #2e8eda; */ | ||
+ | } | ||
+ | |||
+ | |||
+ | .box { | ||
+ | border-bottom:1px solid #e9e9e9; | ||
+ | padding-bottom:20px; | ||
+ | } | ||
+ | |||
+ | .flexslider .slide-caption { | ||
+ | width: 90%; | ||
+ | padding: 2%; | ||
+ | position: absolute; | ||
+ | left: 0; | ||
+ | bottom: -40px; | ||
+ | } | ||
+ | |||
+ | |||
+ | #inner-headline .breadcrumb { | ||
+ | float:left; | ||
+ | clear:both; | ||
+ | width:100%; | ||
+ | } | ||
+ | |||
+ | .breadcrumb > li { | ||
+ | font-size:13px; | ||
+ | } | ||
+ | |||
+ | |||
+ | ul.portfolio li article a i.icon-48{ | ||
+ | width:20px; | ||
+ | height:20px; | ||
+ | font-size:16px; | ||
+ | line-height:20px; | ||
+ | } | ||
+ | |||
+ | |||
+ | .left-sidebar{ | ||
+ | border-right:none; | ||
+ | padding:0 0 0 0; | ||
+ | border-bottom: 1px dotted #e6e6e6; | ||
+ | padding-bottom:10px; | ||
+ | margin-bottom:40px; | ||
+ | } | ||
+ | |||
+ | .right-sidebar{ | ||
+ | margin-top:30px; | ||
+ | border-left:none; | ||
+ | padding:0 0 0 0; | ||
+ | } | ||
+ | |||
+ | |||
+ | footer .col-lg-1, footer .col-lg-2, footer .col-lg-3, footer .col-lg-4, footer .col-lg-5, footer .col-lg-6, | ||
+ | footer .col-lg-7, footer .col-lg-8, footer .col-lg-9, footer .col-lg-10, footer .col-lg-11, footer .col-lg-12{ | ||
+ | margin-bottom:20px; | ||
+ | } | ||
+ | |||
+ | #sub-footer ul.social-network { | ||
+ | float:left; | ||
+ | } | ||
+ | |||
+ | |||
+ | |||
+ | [class*="span"] { | ||
+ | margin-bottom:20px; | ||
+ | } | ||
+ | |||
+ | } | ||
+ | |||
+ | @media (max-width: 480px) { | ||
+ | .bottom-article a.pull-right { | ||
+ | float:left; | ||
+ | margin-top:20px; | ||
+ | } | ||
+ | |||
+ | |||
+ | .search{ | ||
+ | float:left; | ||
+ | } | ||
+ | |||
+ | .flexslider .flex-caption { | ||
+ | display:none; | ||
+ | } | ||
+ | |||
+ | |||
+ | .cta-text { | ||
+ | margin:0 auto; | ||
+ | text-align:center; | ||
+ | } | ||
+ | |||
+ | ul.portfolio li article a i{ | ||
+ | width:20px; | ||
+ | height:20px; | ||
+ | font-size:14px; | ||
+ | } | ||
+ | |||
+ | |||
+ | } | ||
+ | .tab-section .col{ | ||
+ | |||
+ | } | ||
+ | .tab-section .container{ | ||
+ | padding: 15px; | ||
+ | border: 1px solid #eaeaea; | ||
+ | background: #fff; | ||
+ | } | ||
+ | .active .collapsible-header{color: #e91e63;} | ||
+ | span.color { | ||
+ | color: #e91e63; | ||
+ | text-transform: uppercase; | ||
+ | } | ||
+ | .input-field label { | ||
+ | color: #888; | ||
+ | font-size:14px; | ||
+ | } | ||
+ | .input-field { | ||
+ | position: relative; | ||
+ | margin-top: 1rem; | ||
+ | float: left; | ||
+ | width: 100%; | ||
+ | } | ||
+ | .alert-danger { | ||
+ | float: left; | ||
+ | } | ||
+ | |||
+ | |||
+ | /********************** | ||
+ | ****iGEM************************************************************* | ||
+ | **************/ | ||
+ | <script> | ||
+ | if ($('.firstHeading')) $('.firstHeading').remove(); | ||
+ | </script> | ||
+ | <style> | ||
+ | html { | ||
+ | overflow-x: hidden; | ||
+ | width: 100%; | ||
+ | } | ||
+ | |||
+ | .banner-top { | ||
+ | margin-left: 15%; | ||
+ | } | ||
+ | |||
+ | #igem_home_logo { | ||
+ | width: 100%; | ||
+ | margin-top: 80px; | ||
+ | } | ||
+ | |||
+ | @media (min-width: 992px) { | ||
+ | .work-content .col-md-4 { | ||
+ | width: 25%; | ||
+ | margin-right: 8%; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | .carousel-control.left, | ||
+ | .carousel-control.right { | ||
+ | background-image: none; | ||
+ | padding-top: 18%; | ||
+ | } | ||
+ | |||
+ | .carousel-indicators li { | ||
+ | background-color: #fff; | ||
+ | border: 1px solid #a94442; | ||
+ | } | ||
+ | |||
+ | .carousel-indicators li.active { | ||
+ | background-color: #f0ad4e; | ||
+ | } | ||
+ | |||
+ | #contentSub, | ||
+ | #contentcontainer, | ||
+ | #footer-box, | ||
+ | #sitesub, | ||
+ | #catlinks, | ||
+ | #search-controls, | ||
+ | #p-logo, | ||
+ | .h3, | ||
+ | .printfooter, | ||
+ | .firstHeading, | ||
+ | .visualClear { | ||
+ | display: none; | ||
+ | box-sizing: content-box; | ||
+ | } | ||
+ | |||
+ | #sideMenu, | ||
+ | #top_title { | ||
+ | display: none; | ||
+ | box-sizing: content-box; | ||
+ | } | ||
+ | |||
+ | #sideMenu .mainMenu_toggle, | ||
+ | #sideMenu .mainMenu_Title, | ||
+ | #sideMenu .sideMenuTitle, | ||
+ | #sideMenu .expand_subMenus { | ||
+ | box-sizing: content-box; | ||
+ | } | ||
+ | |||
+ | #bodyContent h1, | ||
+ | #bodyContent h2, | ||
+ | #bodyContent h3, | ||
+ | #bodyContent h4, | ||
+ | #bodyContent h5 { | ||
+ | margin-bottom: 0px; | ||
+ | } | ||
+ | |||
+ | #search_text { | ||
+ | color: #000; | ||
+ | } | ||
+ | |||
+ | #globalWrapper, | ||
+ | #content { | ||
+ | position: absolute; | ||
+ | left: 0; | ||
+ | right: 0; | ||
+ | top: 0; | ||
+ | width: 100%; | ||
+ | height: 100%; | ||
+ | border: 0px; | ||
+ | background-color: transparent; | ||
+ | margin: 0px; | ||
+ | padding: 0px; | ||
+ | } | ||
+ | |||
+ | @media (min-width: 1200px) { | ||
+ | .container-fluid .container { | ||
+ | width: 969px; | ||
+ | /* margin-left: 300px; */ | ||
+ | } | ||
+ | .head-container { | ||
+ | width: 1170px; | ||
+ | } | ||
+ | } | ||
+ | /* article */ | ||
+ | |||
+ | .text-content article p { | ||
+ | text-indent: 20px; | ||
+ | margin: 10px 0; | ||
+ | font-size: 15px; | ||
+ | line-height: 24px; | ||
+ | } | ||
+ | |||
+ | .text-content article img { | ||
+ | width: 80%; | ||
+ | padding-left: 20%; | ||
+ | /* margin-left: 20%; */ | ||
+ | } | ||
+ | /* .mw-content-ltr ol{ | ||
+ | margin:0; | ||
+ | } */ | ||
+ | |||
+ | ol.carousel-indicators { | ||
+ | position: absolute; | ||
+ | bottom: 10px; | ||
+ | left: 50%; | ||
+ | z-index: 15; | ||
+ | width: 60%; | ||
+ | padding-left: 0; | ||
+ | margin-left: -30%; | ||
+ | text-align: center; | ||
+ | list-style: none; | ||
+ | } | ||
+ | |||
+ | |||
+ | @charset "utf-8"; | ||
+ | body { | ||
+ | font-family: "Helvetica Neue", Helvetica, Arial, "Microsoft Yahei UI", "Microsoft YaHei", SimHei, "\5B8B\4F53", simsun, sans-serif; | ||
+ | color: #666; | ||
+ | width:100%; | ||
+ | } | ||
+ | .logo { | ||
+ | padding:0; | ||
+ | } | ||
+ | |||
+ | #myCarousel { | ||
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+ | <h2 class="pageTitle">Discription</h2> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
+ | |||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | <div class="col-md-6"> | ||
+ | <div class="about-text"> | ||
+ | <h3><span class="color">Shewanella</span></h3> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <p>In the part of Shewanella, we employ Shewanella oneidensis MR-1 as our chassis and pyydt as the plasmid. Shewanella could utilize the lactate as its carbon source and produce electricity with the help of a multiprotein porin-cytochrome complex on its outer membrane called MtrABC. MtrABC could transfer the electron from Shewanella’s inner membrane to the surrounding. pYYDT is a shuttle plasmid between E.Coli and Shewanella. We constructed this plasmid in E.Coli and transferred it into Shewanella by conjugating these two kinds of bacteria.</p> | ||
+ | <div class="col-md-6"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b6/T--HUST-China--2018-description-logo1.png" alt="showcase image"> | ||
+ | </div> | ||
+ | |||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <p>Our design of constructing Shewanella is devided into two parts: improvement of utilization of lactate and increment of the amount of NADH in Shewanella.</p> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <p>(1). Improvement of utilization of lactate: | ||
+ | To make the use of lactate more efficiently, we overexpress four genes: dld-Ⅱ, lldE, lldF, lldG.</p> | ||
+ | </div> | ||
+ | <p>①. dld-Ⅱ: dld-Ⅱ refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. </p> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <p>②. lldE/lldF/lldG: They could encode a L-lactate dehydrogenase complex which could catalyze D-lactate’s transformation into pyruvate. | ||
+ | We constructed them separately in pYYDT and found out if there was any improvement at the amount of electricty produced by our Shewanella.</p> | ||
+ | <div class="col-md-6"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/eb/T--HUST-China--2018-description-logo2.png" alt="showcase image"> | ||
+ | |||
+ | </div> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/6/66/T--HUST-China--2018-description-logo3.png" alt="showcase image"> | ||
+ | </div> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <p>Then we put these four genes in one circuit to level up the production of electricity.</p> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/4b/T--HUST-China--2018-description-logo4.png" alt="showcase image"> | ||
+ | </div> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <p>(2). Increment of the amount of NADH in Shewanella</p> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <p>To achieve this goal, we overexpress these four genes: gapA2, mdh, pflB, fdh*. We chose them according to our reference.</p> | ||
+ | <p>①. gapA2: It encodes glyceraldehyde-3-phosphate dehydrogenase which could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate</p> | ||
+ | <p>②. mdh: It encodes NAD dependent malate dehydrogenase which transforms malate into pyruvate</p> | ||
+ | <p>③. pflB: It encodes pyruvate formate-lyase to transform pyruvate into Acetyl-CoA</p> | ||
+ | <p>③. pflB: It encodes pyruvate formate-lyase to transform pyruvate into Acetyl-CoA</p> | ||
+ | <div class="col-md-6"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/26/T--HUST-China--2018-description-logo5.png" alt="showcase image"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b7/T--HUST-China--2018-description-logo6.png" alt="showcase image"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/f/f0/T--HUST-China--2018-description-logo7.png" alt="showcase image"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/2a/T--HUST-China--2018-description-logo8.png" alt="showcase image"> | ||
+ | </div> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <p>According to our model, we divided these four genes into two groups: gapA2/mdh and pflB/fdh*. </p> | ||
+ | <div class="col-md-6"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/23/T--HUST-China--2018-description-logo9.png" alt="showcase image"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/47/T--HUST-China--2018-description-logo10.png" alt="showcase image"> | ||
+ | </div> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <p>Finally, we put all these genes into one circuit and found out if the amount of electricity could reach the maximum. </p> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/82/T--HUST-China--2018-description-logo11.png" alt="showcase image"> | ||
+ | |||
+ | |||
+ | |||
+ | |||
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Revision as of 13:30, 16 September 2018
Discription
Shewanella
In the part of Shewanella, we employ Shewanella oneidensis MR-1 as our chassis and pyydt as the plasmid. Shewanella could utilize the lactate as its carbon source and produce electricity with the help of a multiprotein porin-cytochrome complex on its outer membrane called MtrABC. MtrABC could transfer the electron from Shewanella’s inner membrane to the surrounding. pYYDT is a shuttle plasmid between E.Coli and Shewanella. We constructed this plasmid in E.Coli and transferred it into Shewanella by conjugating these two kinds of bacteria.
Our design of constructing Shewanella is devided into two parts: improvement of utilization of lactate and increment of the amount of NADH in Shewanella.
(1). Improvement of utilization of lactate: To make the use of lactate more efficiently, we overexpress four genes: dld-Ⅱ, lldE, lldF, lldG.
①. dld-Ⅱ: dld-Ⅱ refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate.
②. lldE/lldF/lldG: They could encode a L-lactate dehydrogenase complex which could catalyze D-lactate’s transformation into pyruvate. We constructed them separately in pYYDT and found out if there was any improvement at the amount of electricty produced by our Shewanella.
Then we put these four genes in one circuit to level up the production of electricity.
(2). Increment of the amount of NADH in Shewanella
To achieve this goal, we overexpress these four genes: gapA2, mdh, pflB, fdh*. We chose them according to our reference.
①. gapA2: It encodes glyceraldehyde-3-phosphate dehydrogenase which could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate
②. mdh: It encodes NAD dependent malate dehydrogenase which transforms malate into pyruvate
③. pflB: It encodes pyruvate formate-lyase to transform pyruvate into Acetyl-CoA
③. pflB: It encodes pyruvate formate-lyase to transform pyruvate into Acetyl-CoA
According to our model, we divided these four genes into two groups: gapA2/mdh and pflB/fdh*.
Finally, we put all these genes into one circuit and found out if the amount of electricity could reach the maximum.