Difference between revisions of "Team:HUST-China/Description"

(Prototype team page)
 
Line 1: Line 1:
{{HUST-China}}
+
<html lang="en">
<html>
+
<head>
 +
<meta charset="utf-8">
 +
<title>HillSide Multi purpose HTML5 Template</title>
 +
<meta name="viewport" content="width=device-width, initial-scale=1.0" />
 +
<meta name="description" content="" />
 +
<meta name="author" content="http://webthemez.com" />
 +
<!-- css -->
 +
<link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
 +
<link rel="stylesheet" href="https://2018.igem.org/Team:HUST-China/materialize/css/materialize?action=raw&amp;ctype=text/css" media="screen,projection" />
 +
<link href="https://2018.igem.org/Team:HUST-China/css/bootstrap?action=raw&amp;ctype=text/css" rel="stylesheet" />
 +
<link href="https://2018.igem.org/Team:HUST-China/css/fancybox/jquery?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
<link href="https://2018.igem.org/Team:HUST-China/css/flexslider?action=raw&amp;ctype=text/css" rel="stylesheet" />
 +
<!--slider-->
 +
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:HUST-China/css/zoomslider?action=raw&amp;ctype=text/css" />
  
 +
<link href="https://2018.igem.org/Team:HUST-China/css/font-awesome?action=raw&amp;ctype=text/css" rel="stylesheet" />
 +
<link href="https://2018.igem.org/Team:HUST-China/css/animate?action=raw&amp;ctype=text/css" rel="stylesheet" />
  
 +
<!--<link href="https://2018.igem.org/Team:HUST-China/css/style?action=raw&amp;ctype=text/css" rel="stylesheet" /> -->
 +
<style type="text/css">
 +
/*
 +
Author URI: http://webthemez.com/
 +
Note: Licence under Creative Commons Attribution 3.0 
 +
-------------------------------------------------------*/
  
<div class="column full_size">
+
@import url('http://fonts.googleapis.com/css?family=Noto+Serif:400,400italic,700|Open+Sans:400,600,700');
<h1>Description</h1>
+
@import url('font-awesome.css'); 
 +
@import url('animate.css');
  
<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
+
body {
 +
font-family:'Open Sans', Arial, sans-serif;
 +
font-weight:300;
 +
line-height:1.6em;
 +
color:#656565;
 +
font-size: 1.4em;
 +
background: #ffffff;
 +
}
 +
a {
 +
    color: #0a0a0a;
 +
    text-decoration: none;
 +
}
 +
a:active {
 +
outline:0;
 +
}
  
</div>
+
.clear {
 +
clear:both;
 +
}
  
 +
h1,h2{
 +
font-weight: 600;
 +
line-height:1.1em;
 +
color: #e91e63;
 +
margin-bottom: 20px;
 +
text-transform: uppercase;
 +
}
 +
h3, h4, h5, h6 {
 +
line-height:1.1em;
 +
color: #212121;
 +
margin-bottom: 20px;
 +
}
 +
input, button, select, textarea {
 +
    border-radius: 0px !important;
 +
}
 +
.timetable{background: #2ab975;padding: 5px 23px;}
 +
.timetable hr{
 +
color:#fff;
 +
padding-top: 29px;
 +
margin-bottom: 20px !important;
 +
}
 +
.timetable dl{
 +
color:#fff;
 +
}
 +
.timetable dt{
 +
color:#fff;
 +
}
 +
.timetable dd{
 +
color:#fff;
 +
}
 +
#wrapper{
 +
width:100%;
 +
margin:0;
 +
padding:0;
 +
}
 +
ul.withArrow{
 +
 +
}
 +
ul.withArrow li{
 +
 +
}
 +
ul.withArrow li span{
 +
 +
margin-right: 10px;
 +
 +
font-weight: 700;
 +
 +
color: #0e0e0e;
 +
 +
border: 1px solid;
 +
 +
padding: 4px 8px;
 +
 +
font-size: 11px;
 +
 +
border-radius: 50%;
 +
 +
height: 20px;
 +
 +
width: 20px;
 +
}
 +
.widget {
 +
    color: #858585;
 +
}
 +
.row,.row-fluid {
 +
margin-bottom:30px;
 +
}
  
 +
.row .row,.row-fluid .row-fluid{
 +
margin-bottom:30px;
 +
}
  
<div class="column two_thirds_size">
+
.row.nomargin,.row-fluid.nomargin {
<h3>What should this page contain?</h3>
+
margin-bottom:0;
<ul>
+
}
<li> A clear and concise description of your project.</li>
+
.about-image img {
<li>A detailed explanation of why your team chose to work on this particular project.</li>
+
    width: 100%;
<li>References and sources to document your research.</li>
+
}
<li>Use illustrations and other visual resources to explain your project.</li>
+
img.img-polaroid {
</ul>
+
margin:0 0 20px 0;
</div>
+
}
 +
.img-box {
 +
max-width:100%;
 +
}
 +
.navbar-default .navbar-toggle {
 +
    border-color: #ddd;
 +
    margin-top: 16px;
 +
}
 +
/*  Header
 +
==================================== */
 +
.topbar{
 +
background-color: #e91e63;
 +
padding: 0;
 +
/* color:#fff; */
 +
}
 +
.topbar .container .row {
 +
    margin: 0;
 +
padding:0;
 +
}
 +
.topbar .container .row .col-md-12 {
 +
padding:0;
 +
}
 +
.topbar p{
 +
margin:0;
 +
display:inline-block;
 +
font-size: 13px;
 +
color: #f1f6ff;
 +
}
 +
.topbar p > i{
 +
margin-right:5px;
 +
}
 +
.topbar p:last-child{
 +
text-align:right;
 +
}
 +
header .navbar {
 +
    margin-bottom: 0;
 +
}
 +
.topbar li.topbar {
 +
    display: inline;
 +
    padding-right: 18px;
 +
    line-height: 52px;
 +
    transition: all .3s linear;
 +
}
  
<div class="column third_size" >
+
.topbar li.topbar:hover {
<div class="highlight decoration_A_full">
+
    color: #1bbde8;
<h3>Inspiration</h3>
+
}
<p>See how other teams have described and presented their projects: </p>
+
  
<ul>
+
.topbar ul.info i {
<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
+
    color: #131313;
<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
+
    font-size: 16px;
<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
+
    font-style: normal;
<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
+
    margin-right: 8px;
</ul>
+
    display: inline-block;
</div>
+
    position: relative;
</div>
+
    top: 4px;
 +
}
  
 +
.topbar ul.info span {
 +
    /* line-height: 42px; */
 +
    /* display: inline-block; */
 +
}
  
 +
.topbar ul.info li {
 +
    float: right;
 +
    padding-left: 30px;
 +
    color: #ffffff;
 +
    font-size: 13px;
 +
    line-height: 44px;
 +
}
  
 +
.topbar ul.info i span {
 +
    color: #aaa;
 +
    font-size: 13px;
 +
    font-family: "Rpboto", sans-serif;
 +
    font-weight: 400;
 +
    line-height: 50px;
 +
    padding-left: 18px;
 +
}
 +
.navbar-default {
 +
    border: none;
 +
}
  
<div class="column two_thirds_size" >
+
.navbar-brand {
<h3>Advice on writing your Project Description</h3>
+
    color: #222;
 +
    text-transform: uppercase;
 +
    font-size: 24px;
 +
    font-weight: 700;
 +
    line-height: 1em;
 +
    letter-spacing: -1px;
 +
    margin-top: 21px;
 +
    padding: 0 0 0 15px;
 +
}
 +
.navbar-default .navbar-brand{color: #e91e63;font-size: 24px;text-transform: uppercase;}
 +
.navbar-default .navbar-brand:hover {
 +
    color: #fff;
 +
}
 +
header .navbar-collapse  ul.navbar-nav {
 +
    float: right;
 +
    margin-right: 0;
 +
}
 +
header .navbar {min-height: 70px;padding: 18px 0;background: #1b1b1b;}
 +
.home-page header .navbar-default{
 +
    background: #1b1b1b;
 +
    /* position: absolute; */
 +
    width: 100%;
 +
}
  
<p>
+
header .nav li a:hover,
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.  
+
header .nav li a:focus,
</p>
+
header .nav li.active a,
 +
header .nav li.active a:hover,
 +
header .nav li a.dropdown-toggle:hover,
 +
header .nav li a.dropdown-toggle:focus,
 +
header .nav li.active ul.dropdown-menu li a:hover,
 +
header .nav li.active ul.dropdown-menu li.active a{
 +
    -webkit-transition: all .3s ease;
 +
    -moz-transition: all .3s ease;
 +
    -ms-transition: all .3s ease;
 +
    -o-transition: all .3s ease;
 +
    transition: all .3s ease;
 +
}
  
</div>
 
  
<div class="column third_size">
+
header .navbar-default .navbar-nav > .open > a,
<h3>References</h3>
+
header .navbar-default .navbar-nav > .open > a:hover,
<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
+
header .navbar-default .navbar-nav > .open > a:focus {
 +
    -webkit-transition: all .3s ease;
 +
    -moz-transition: all .3s ease;
 +
    -ms-transition: all .3s ease;
 +
    -o-transition: all .3s ease;
 +
    transition: all .3s ease;
 +
}
  
</div>
 
  
 +
header .navbar {
 +
    min-height: 62px;
 +
    padding: 0;
 +
}
  
 +
header .navbar-nav > li  {
 +
    padding-bottom: 12px;
 +
    padding-top: 12px;
 +
    padding: 0 !important;
 +
}
  
 +
header  .navbar-nav > li > a {
 +
    /* padding-bottom: 6px; */
 +
    /* padding-top: 5px; */
 +
    margin-left: 2px;
 +
    line-height: 40px;
 +
    font-weight: 700;
 +
    -webkit-transition: all .3s ease;
 +
    -moz-transition: all .3s ease;
 +
    -ms-transition: all .3s ease;
 +
    -o-transition: all .3s ease;
 +
    transition: all .3s ease;
 +
}
 +
section.dishes h2 {
 +
    color: #fff;
 +
}
 +
.services{
 +
 +
}
 +
.dishes{
 +
 +
background: #E91E63;
 +
 +
padding: 35px 0;
 +
 +
color: #ffffff;
 +
}
 +
.dropdown-menu li a:hover {
 +
    color: #fff !important;
 +
    background: #e91e63 !important;
 +
}
  
 +
header .nav .caret {
 +
    border-bottom-color: #e91e63;
 +
    border-top-color: #e91e63;
 +
}
 +
.navbar-default .navbar-nav > .active > a,
 +
.navbar-default .navbar-nav > .active > a:hover,
 +
.navbar-default .navbar-nav > .active > a:focus {
 +
  background-color: #fff;
 +
}
 +
.navbar-default .navbar-nav > .open > a,
 +
.navbar-default .navbar-nav > .open > a:hover,
 +
.navbar-default .navbar-nav > .open > a:focus {
 +
  background-color: #e91e63;
 +
  color: #fff;
 +
}
 +
  
 +
.dropdown-menu  {
 +
    box-shadow: none;
 +
    border-radius: 0;
 +
border: none;
 +
}
  
 +
.dropdown-menu li:last-child  {
 +
padding-bottom: 0 !important;
 +
margin-bottom: 0;
 +
}
 +
 +
header .nav li .dropdown-menu  {
 +
  padding: 0;
 +
}
 +
 +
header .nav li .dropdown-menu li a {
 +
  line-height: 28px;
 +
  padding: 3px 12px;
 +
}
 +
 +
/* --- menu --- */
 +
.flex-caption p {
 +
    line-height: 22px;
 +
    font-weight: 300;
 +
    color: #ffffff;
 +
    text-transform: capitalize;
 +
}
 +
header .navigation {
 +
float:right;
 +
}
 +
 +
header ul.nav li {
 +
border:none;
 +
margin:0;
 +
}
 +
 +
header ul.nav li a {
 +
font-size: 13px;
 +
border:none;
 +
font-weight: 600;
 +
text-transform:uppercase;
 +
}
 +
 +
header ul.nav li ul li a {
 +
font-size:12px;
 +
border:none;
 +
font-weight:300;
 +
text-transform:uppercase;
 +
}
 +
 +
 +
.navbar .nav > li > a {
 +
  color: #9a9a9a;
 +
  text-shadow: none;
 +
  border: 1px solid rgba(255, 255, 255, 0) !important;
 +
}
 +
 +
.navbar .nav a:hover {
 +
background:none;
 +
color: #2e8eda;
 +
}
 +
 +
.navbar .nav > .active > a,.navbar .nav > .active > a:hover {
 +
background:none;
 +
font-weight:700;
 +
}
 +
 +
.navbar .nav > .active > a:active,.navbar .nav > .active > a:focus {
 +
background:none;
 +
outline:0;
 +
font-weight:700;
 +
}
 +
 +
.navbar .nav li .dropdown-menu {
 +
z-index:2000;
 +
}
 +
 +
header ul.nav li ul {
 +
margin-top:1px;
 +
}
 +
header ul.nav li ul li ul {
 +
margin:1px 0 0 1px;
 +
}
 +
.dropdown-menu .dropdown i {
 +
position:absolute;
 +
right:0;
 +
margin-top:3px;
 +
padding-left:20px;
 +
}
 +
 +
.navbar .nav > li > .dropdown-menu:before {
 +
  display: inline-block;
 +
  border-right: none;
 +
  border-bottom: none;
 +
  border-left: none;
 +
  border-bottom-color: none;
 +
  content:none;
 +
}
 +
.navbar-default .navbar-nav>.active>a, .navbar-default .navbar-nav>.active>a:hover, .navbar-default .navbar-nav>.active>a:focus {color: #ffffff;background: #10cdff;border-radius: 0;}
 +
.navbar-default .navbar-nav>li>a:hover, .navbar-default .navbar-nav>li>a:focus {
 +
  color: #ffffff;
 +
  background-color: transparent;
 +
}
 +
 +
ul.nav li.dropdown a {
 +
z-index:1000;
 +
display:block;
 +
}
 +
 +
select.selectmenu {
 +
display:none;
 +
}
 +
.pageTitle{color: #fff;margin: 30px 0 3px;display: inline-block;font-size: 28px;font-weight: 700;text-transform: uppercase;}
 +
 +
#banner{
 +
width: 100%;
 +
background:#000;
 +
position:relative;
 +
margin:0;
 +
padding:0;
 +
}
 +
 +
/*  Sliders
 +
==================================== */
 +
/* --- flexslider --- */
 +
#main-slider:before {content: '';width: 100%;height: 100%;background: rgba(0, 0, 0, 0.65);z-index: 1;position: absolute;background: rgba(30, 30, 30, 0.31);}
 +
.flex-direction-nav a{
 +
display:none;
 +
}
 +
.flexslider {
 +
padding:0; 
 +
background: #fff;
 +
position: relative;
 +
zoom: 1;
 +
}
 +
.flex-direction-nav .flex-prev{
 +
left:0px;
 +
}
 +
.flex-direction-nav .flex-next{
 +
right:0px;
 +
}
 +
.flex-caption {zoom: 1;bottom: 198px;background-color: transparent;color: #fff;margin: 0;padding: 2px 25px 0px 30px;/* position: absolute; */left: 0;text-align: center;margin: 0 auto;right: 0px;display: inline-block;bottom: 10%;width: 50%;right: 0 !important;border-radius: 15px 15px 0 0;}
 +
.flex-caption h3 {color: #fff;letter-spacing: 0px;margin-bottom: 8px;text-transform: uppercase;font-size: 70px;font-weight: 700;}
 +
.flex-caption p {margin: 12px 0 18px;font-size: 20px;font-weight: 400;}
 +
.skill-home{
 +
margin-bottom:50px;
 +
}
 +
.c1{
 +
border: #ed5441 1px solid;
 +
background:#ed5441;
 +
}
 +
.c2{
 +
border: #24c4db 1px solid;
 +
background:#24c4db;
 +
}
 +
.c3{
 +
border: #1891EC 1px solid;
 +
background:#1891EC;
 +
}
 +
.c4{
 +
border: #609cec 1px solid;
 +
background:#609cec;
 +
}
 +
.skill-home .icons {padding: 38px;width: 94px;height: 94px;color: #fff;font-size: 42px;font-size: 38px;text-align: center;-ms-border-radius: 50%;-moz-border-radius: 50%;-webkit-border-radius: 50%;border-radius: 0;display: inline-table;border: 4px solid #efefef;color: #ff5722;background: transparent;border-radius: 50%;background: #ffffff;}
 +
.skill-home h2 {
 +
padding-top: 20px;
 +
font-size: 36px;
 +
font-weight: 700;
 +
}
 +
.testimonial-solid {
 +
padding: 50px 0 60px 0;
 +
margin: 0 0 0 0;
 +
background: #FFFFFF;
 +
text-align: center;
 +
}
 +
.testi-icon-area {
 +
text-align: center;
 +
position: absolute;
 +
top: -84px;
 +
margin: 0 auto;
 +
width: 100%;
 +
}
 +
.testi-icon-area .quote {
 +
padding: 15px 0 0 0;
 +
margin: 0 0 0 0;
 +
background: #ffffff;
 +
text-align: center;
 +
color: #1891EC;
 +
display: inline-table;
 +
width: 70px;
 +
height: 70px;
 +
-ms-border-radius: 50%;
 +
-moz-border-radius: 50%;
 +
-webkit-border-radius: 50%;
 +
border-radius: 50%;
 +
font-size: 42px;
 +
border: 1px solid #1891EC;
 +
display: none;
 +
}
 +
 +
.testi-icon-area .carousel-inner {
 +
margin: 20px 0;
 +
}
 +
.carousel-indicators {
 +
bottom: -30px;
 +
}
 +
.text-center img {
 +
margin: auto;
 +
}
 +
.aboutUs{
 +
padding:40px 0;
 +
background: #F2F2F2;
 +
}
 +
img.img-center {
 +
margin: 0 auto;
 +
display: block;
 +
max-width: 100%;
 +
}
 +
.home-about{
 +
padding: 60px 0 0px;
 +
}
 +
.home-about hr{
 +
padding:0;
 +
margin:0;
 +
}
 +
.home-about .info-box{
 +
 +
}
 +
.projects {
 +
    padding: 60px 0;
 +
    background: #1b1b1b;
 +
    color: #a7a7a7;
 +
    border-top: 4px solid #e91e63;
 +
}
 +
 +
.card-content .price {
 +
    font-size: 14px;
 +
    color: #e91e63;
 +
}
 +
 +
.projects h4 {
 +
    font-size: 16px;
 +
    margin: 8px 0;
 +
}
 +
 +
.projects h5 {
 +
    font-weight: bold;
 +
    font-size: 14px;
 +
    margin: 0;
 +
}
 +
/* Testimonial
 +
----------------------------------*/
 +
.testimonial-area {
 +
padding: 0 0 0 0;
 +
margin:0;
 +
background: url(../img/low-poly01.jpg) fixed center center;
 +
background-size: cover;
 +
-webkit-background-size: cover;
 +
-moz-background-size: cover;
 +
-ms-background-size: cover;
 +
}
 +
.testimonial-solid p {
 +
color: #000000;
 +
font-size: 16px;
 +
line-height: 30px;
 +
font-style: italic;
 +
}
 +
section.hero-text {
 +
background: #F4783B;
 +
padding:50px 0 50px 0;
 +
color: #fff;
 +
}
 +
section.hero-text h1{
 +
color: #fff;
 +
}
 +
 +
/* Clients
 +
------------------------------------ */
 +
#clients {
 +
  padding: 60px 0;
 +
  }
 +
  #clients .client .img {
 +
    height: 76px;
 +
    width: 138px;
 +
    cursor: pointer;
 +
    -webkit-transition: box-shadow .1s linear;
 +
    -moz-transition: box-shadow .1s linear;
 +
    transition: box-shadow .1s linear; }
 +
    #clients .client .img:hover {
 +
      cursor: pointer;
 +
      /*box-shadow: 0px 0px 2px 0px rgb(155, 155, 155);*/
 +
      border-radius: 8px; }
 +
  #clients .client .client1 {
 +
    background: url("../img/client1.png") 0 -75px; }
 +
    #clients .client .client1:hover {
 +
      background-position: 1px 0px; }
 +
  #clients .client .client2 {
 +
    background: url("../img/client2.png") 0 -75px; }
 +
    #clients .client .client2:hover {
 +
      background-position: -1px 0px; }
 +
  #clients .client .client3 {
 +
    background: url("../img/client3.png") 0 -76px; }
 +
    #clients .client .client3:hover {
 +
      background-position: 0px 0px; }
 +
 +
 +
/* Content
 +
==================================== */
 +
 +
#content {
 +
position:relative;
 +
background: #ffffff;
 +
padding:50px 0 0px 0;
 +
}
 +
 +
.cta-text {
 +
text-align: center;
 +
margin-top:10px;
 +
}
 +
 +
 +
.big-cta .cta {
 +
margin-top:10px;
 +
}
 +
 +
.box {
 +
width: 100%;
 +
}
 +
.box-gray  {
 +
background: #f8f8f8;
 +
padding: 20px 20px 30px;
 +
}
 +
.box-gray  h4,.box-gray  i {
 +
margin-bottom: 20px;
 +
}
 +
.box-bottom {
 +
padding: 20px 0;
 +
text-align: center;
 +
}
 +
.box-bottom a {
 +
color: #fff;
 +
font-weight: 700;
 +
}
 +
.box-bottom a:hover {
 +
color: #eee;
 +
text-decoration: none;
 +
}
 +
 +
 +
/* Bottom
 +
==================================== */
 +
 +
#bottom {
 +
background:#fcfcfc;
 +
padding:50px 0 0;
 +
 +
}
 +
/* twitter */
 +
#twitter-wrapper {
 +
    text-align: center;
 +
    width: 70%;
 +
    margin: 0 auto;
 +
}
 +
#twitter em {
 +
    font-style: normal;
 +
    font-size: 13px;
 +
}
 +
 +
#twitter em.twitterTime a {
 +
font-weight:600;
 +
}
 +
 +
#twitter ul {
 +
    padding: 0;
 +
list-style:none;
 +
}
 +
#twitter ul li {
 +
    font-size: 20px;
 +
    line-height: 1.6em;
 +
    font-weight: 300;
 +
    margin-bottom: 20px;
 +
    position: relative;
 +
    word-break: break-word;
 +
}
 +
 +
 +
/* page headline
 +
==================================== */
 +
 +
#inner-headline{background: #e4e4e4;position: relative;margin: 0;padding: 0;color: #1891EC;}
 +
 +
 +
#inner-headline h2.pageTitle{
 +
color: #e91e63;
 +
padding: 5px 0;
 +
display:block;
 +
text-align: center;
 +
}
 +
 +
/* --- breadcrumbs --- */
 +
#inner-headline ul.breadcrumb {
 +
margin:40px 0;
 +
float:left;
 +
}
 +
 +
#inner-headline ul.breadcrumb li {
 +
margin-bottom:0;
 +
padding-bottom:0;
 +
}
 +
#inner-headline ul.breadcrumb li {
 +
font-size:13px;
 +
color:#fff;
 +
}
 +
 +
#inner-headline ul.breadcrumb li i{
 +
color:#dedede;
 +
}
 +
 +
#inner-headline ul.breadcrumb li a {
 +
color:#fff;
 +
}
 +
 +
ul.breadcrumb li a:hover {
 +
text-decoration:none;
 +
}
 +
 +
/* Forms
 +
============================= */
 +
 +
/* --- contact form  ---- */
 +
form#contactform input[type="text"] {
 +
  width: 100%;
 +
  border: 1px solid #f5f5f5;
 +
  min-height: 40px;
 +
  padding-left:20px;
 +
  font-size:13px;
 +
  padding-right:20px;
 +
  -webkit-box-sizing: border-box;
 +
    -moz-box-sizing: border-box;
 +
          box-sizing: border-box;
 +
 +
}
 +
 +
form#contactform textarea {
 +
border: 1px solid #f5f5f5;
 +
  width: 100%;
 +
  padding-left:20px;
 +
  padding-top:10px;
 +
  font-size:13px;
 +
  padding-right:20px;
 +
  -webkit-box-sizing: border-box;
 +
    -moz-box-sizing: border-box;
 +
          box-sizing: border-box;
 +
 +
}
 +
 +
form#contactform .validation {
 +
font-size:11px;
 +
}
 +
 +
#sendmessage {
 +
border:1px solid #e6e6e6;
 +
background:#f6f6f6;
 +
display:none;
 +
text-align:center;
 +
padding:15px 12px 15px 65px;
 +
margin:10px 0;
 +
font-weight:600;
 +
margin-bottom:30px;
 +
 +
}
 +
 +
#sendmessage.show,.show  {
 +
display:block;
 +
}
 +
 +
form#commentform input[type="text"] {
 +
  width: 100%;
 +
  min-height: 40px;
 +
  padding-left:20px;
 +
  font-size:13px;
 +
  padding-right:20px;
 +
  -webkit-box-sizing: border-box;
 +
    -moz-box-sizing: border-box;
 +
          box-sizing: border-box;
 +
-webkit-border-radius: 2px 2px 2px 2px;
 +
-moz-border-radius: 2px 2px 2px 2px;
 +
border-radius: 2px 2px 2px 2px;
 +
 +
}
 +
 +
form#commentform textarea {
 +
  width: 100%;
 +
  padding-left:20px;
 +
  padding-top:10px;
 +
  font-size:13px;
 +
  padding-right:20px;
 +
  -webkit-box-sizing: border-box;
 +
    -moz-box-sizing: border-box;
 +
          box-sizing: border-box;
 +
-webkit-border-radius: 2px 2px 2px 2px;
 +
-moz-border-radius: 2px 2px 2px 2px;
 +
border-radius: 2px 2px 2px 2px;
 +
}
 +
 +
 +
/* --- search form --- */
 +
.search{
 +
float:right;
 +
margin:35px 0 0;
 +
padding-bottom:0;
 +
}
 +
 +
#inner-headline form.input-append {
 +
margin:0;
 +
padding:0;
 +
}
 +
 +
.about-text h3 {
 +
font-size: 18px;
 +
padding-bottom: 0;
 +
font-weight: bold;
 +
}
 +
 +
.about-text .btn {
 +
margin-top: 20px;
 +
//font-size: 15px;
 +
//text-align: justify;
 +
}
 +
 +
.about-image img {
 +
}
 +
 +
.about-text ul {
 +
margin-top: 30px;
 +
padding: 0;
 +
}
 +
 +
.about-text li {
 +
margin-bottom: 10px;
 +
//
 +
font-weight: bold;
 +
list-style: none;
 +
}
 +
 +
 +
.about-text-2 img {
 +
border: 5px solid #ddd;
 +
margin-bottom: 20px;
 +
}
 +
 +
.about-text-2 p {
 +
margin-bottom: 20px;
 +
}
 +
 +
#call-to-action {
 +
background: url(../images/bg/about.jpg) no-repeat;
 +
background-attachment: fixed;
 +
background-position: top center;
 +
padding: 100px 0;
 +
color: #fff;
 +
}
 +
 +
.call-to-action h3 {
 +
font-size: 40px;
 +
letter-spacing: 15px;
 +
color: #fff;
 +
padding-bottom: 30px;
 +
}
 +
 +
.call-to-action p {
 +
color: #999;
 +
padding-bottom: 20px;
 +
}
 +
 +
.call-to-action .btn-primary {
 +
background-color: transparent;
 +
border-color: #999;
 +
}
 +
 +
.call-to-action .btn-primary:hover {
 +
background-color: #151515;
 +
color: #999;
 +
border-color: #151515;
 +
}
 +
 +
#call-to-action-2 {
 +
background-color: #efefef;
 +
padding: 50px 0;
 +
}
 +
#call-to-action-2 h3 {
 +
color: #05142b;
 +
font-size: 24px;
 +
font-weight: 600;
 +
text-transform: uppercase;
 +
}
 +
#call-to-action-2 p {
 +
color: #5f5f5f;
 +
font-size: 15px;
 +
}
 +
#call-to-action-2 .btn-primary {
 +
border-color: #FFFFFF;
 +
/* background: transparent; */
 +
margin-top: 50px;
 +
color: #fff;
 +
}
 +
section.section-padding.gray-bg {
 +
    background: #ffffff;
 +
    padding: 60px;
 +
    color: #484848;
 +
}
 +
 +
  #accordion-alt3 .panel-heading h4 {
 +
font-size: 13px;
 +
line-height: 28px;
 +
}
 +
.panel .panel-heading h4 {
 +
font-weight: 400;
 +
}
 +
.panel-title {
 +
margin-top: 0;
 +
margin-bottom: 0;
 +
font-size: 15px;
 +
color: inherit;
 +
}
 +
.panel-group .panel {
 +
margin-bottom: 0;
 +
border-radius: 2px;
 +
}
 +
.panel {margin-bottom: 18px;background-color: #FFFFFF;border: 1px solid #f3f3f3;border-radius: 2px;-webkit-box-shadow: 0 1px 1px rgba(0,0,0,0.05);box-shadow: 0 1px 1px rgba(0,0,0,0.05);}
 +
#accordion-alt3 .panel-heading h4 a i {font-size: 17px;line-height: 18px;width: 18px;height: 18px;margin-right: 8px;color: #A0A0A0;text-align: center;border-radius: 50%;margin-left: 6px;font-weight: bold;} 
 +
.progress.pb-sm {
 +
height: 6px!important;
 +
}
 +
.progress {
 +
box-shadow: inset 0 0 2px rgba(0,0,0,.1);
 +
}
 +
.progress {
 +
overflow: hidden;
 +
height: 18px;
 +
margin-bottom: 18px;
 +
background-color: #f5f5f5;
 +
border-radius: 2px;
 +
-webkit-box-shadow: inset 0 1px 2px rgba(0,0,0,0.1);
 +
box-shadow: inset 0 1px 2px rgba(0,0,0,0.1);
 +
}
 +
.progress .progress-bar.progress-bar-red {
 +
background: #ed5441;
 +
}
 +
.progress .progress-bar.progress-bar-green {
 +
background: #51d466;
 +
}
 +
.progress .progress-bar.progress-bar-lblue {
 +
background: #32c8de;
 +
}
 +
/* --- portfolio detail --- */
 +
.top-wrapper {
 +
margin-bottom:20px;
 +
}
 +
.info-blocks {margin-bottom: 15px;/* background: #efefef; */}
 +
.info-blocks i.icon-info-blocks {float: left;color: #e91e63;font-size: 40px;min-width: 50px;margin-top: 11px;text-align: center;background: #f5f5f5;width: 72px;height: 72px;/* padding-top: 21px; *//* border: 1px solid #e0e0e0; */line-height: 75px;}
 +
.info-blocks .info-blocks-in {padding: 15px;overflow: hidden;background: #f5f5f5;}
 +
.info-blocks .info-blocks-in h3 {color: #202020;font-size: 20px;line-height: 28px;margin:0px;margin-bottom: 8px;/* font-weight: bold; */}
 +
.info-blocks .info-blocks-in p {font-size: 14px;}
 +
 
 +
blockquote {
 +
font-size:16px;
 +
font-weight:400;
 +
font-family:'Noto Serif', serif;
 +
font-style:italic;
 +
padding-left:0;
 +
color:#a2a2a2;
 +
line-height:1.6em;
 +
border:none;
 +
}
 +
 +
blockquote cite { display:block; font-size:12px; color:#666; margin-top:10px; }
 +
blockquote cite:before { content:"\2014 \0020"; }
 +
blockquote cite a,
 +
blockquote cite a:visited,
 +
blockquote cite a:visited { color:#555; }
 +
 +
/* --- pullquotes --- */
 +
 +
.pullquote-left {
 +
display:block;
 +
color:#a2a2a2;
 +
font-family:'Noto Serif', serif;
 +
font-size:14px;
 +
line-height:1.6em;
 +
padding-left:20px;
 +
}
 +
 +
.pullquote-right {
 +
display:block;
 +
color:#a2a2a2;
 +
font-family:'Noto Serif', serif;
 +
font-size:14px;
 +
line-height:1.6em;
 +
padding-right:20px;
 +
}
 +
 +
/* --- button --- */
 +
.btn{text-align: center;color: #FFFFFF;background: #e91e63;border-color: #e91e63;width: 120px;border-radius: 0;}
 +
.btn-theme {
 +
color: #fff;
 +
background: transparent;
 +
border: 1px solid #fff;
 +
padding: 12px 30px;
 +
font-weight: bold;
 +
}
 +
a.btn.btn-details {
 +
    float: right;
 +
    margin-top: -34px;
 +
    font-size: 14px;
 +
    height: 34px;
 +
    width: 96px;
 +
    text-transform: capitalize;
 +
}
 +
.btn-theme:hover {
 +
color: #eee;
 +
}
 +
 +
/* --- list style --- */
 +
 +
ul.general {
 +
list-style:none;
 +
margin-left:0;
 +
}
 +
 +
ul.link-list{
 +
margin:0;
 +
padding:0;
 +
list-style:none;
 +
}
 +
 +
ul.link-list li{
 +
margin:0;
 +
padding:2px 0 2px 0;
 +
list-style:none;
 +
}
 +
footer{background: #0e0e0e;}
 +
footer ul.link-list li a{
 +
color: #8C8C8C;
 +
}
 +
footer ul.link-list li a:hover {
 +
color: #e91e63;
 +
}
 +
/* --- Heading style --- */
 +
 +
h4.heading {
 +
font-weight:700;
 +
}
 +
 +
.heading { margin-bottom: 30px; }
 +
 +
.heading {
 +
position: relative;
 +
 +
}
 +
 +
 +
.widgetheading {
 +
width:100%;
 +
padding:0;
 +
font-weight: bold;
 +
}
 +
 +
#bottom .widgetheading {
 +
position: relative;
 +
border-bottom: #e6e6e6 1px solid;
 +
padding-bottom: 9px;
 +
}
 +
 +
aside .widgetheading {
 +
position: relative;
 +
border-bottom: #e9e9e9 1px solid;
 +
padding-bottom: 9px;
 +
}
 +
 +
footer .widgetheading {
 +
position: relative;
 +
}
 +
 +
footer .widget .social-network {
 +
position:relative;
 +
}
 +
 +
.team-member h4{
 +
  text-align: center;
 +
  margin-bottom: 5px;
 +
}
 +
.team-member .deg{
 +
text-align:center;
 +
display:block;
 +
}
 +
#bottom .widget .widgetheading span, aside .widget .widgetheading span, footer .widget .widgetheading span {
 +
position: absolute;
 +
width: 60px;
 +
height: 1px;
 +
bottom: -1px;
 +
right:0;
 +
 +
}
 +
.panel-title>a {
 +
    color: inherit;
 +
    text-decoration: none;
 +
    font-weight: 600;
 +
}
 +
.box-area{padding: 15px;background: #ffffff;margin-top: -21px;border: 2px solid #f3f3f3;}
 +
.box-area h3{font-size: 17px;font-weight: 600;}
 +
/* --- Map --- */
 +
.map{
 +
position:relative;
 +
margin-top:-50px;
 +
margin-bottom:40px;
 +
}
 +
 +
.map iframe{
 +
width:100%;
 +
height:450px;
 +
border:none;
 +
}
 +
 +
.map-grid iframe{
 +
width:100%;
 +
height:350px;
 +
border:none;
 +
margin:0 0 -5px 0;
 +
padding:0;
 +
}
 +
 +
 +
ul.team-detail{
 +
margin:-10px 0 0 0;
 +
padding:0;
 +
list-style:none;
 +
}
 +
 +
ul.team-detail li{
 +
border-bottom:1px dotted #e9e9e9;
 +
margin:0 0 15px 0;
 +
padding:0 0 15px 0;
 +
list-style:none;
 +
}
 +
 +
ul.team-detail li label {
 +
font-size:13px;
 +
}
 +
 +
ul.team-detail li h4, ul.team-detail li label{
 +
margin-bottom:0;
 +
}
 +
 +
ul.team-detail li ul.social-network {
 +
border:none;
 +
margin:0;
 +
padding:0;
 +
}
 +
 +
ul.team-detail li ul.social-network li {
 +
border:none;
 +
margin:0;
 +
}
 +
ul.team-detail li ul.social-network li i {
 +
margin:0;
 +
}
 +
 +
.navbar-brand i {
 +
    color: #ffffff;
 +
    font-size: 26px;
 +
    margin-right: 5px;
 +
    position: relative;
 +
    top: 3px;
 +
}
 +
.pricing-title{
 +
background:#fff;
 +
text-align:center;
 +
padding:10px 0 10px 0;
 +
}
 +
 +
.pricing-title h3{
 +
font-weight:600;
 +
margin-bottom:0;
 +
}
 +
 +
.pricing-offer{
 +
background: #fcfcfc;
 +
text-align: center;
 +
padding:40px 0 40px 0;
 +
font-size:18px;
 +
border-top:1px solid #e6e6e6;
 +
border-bottom:1px solid #e6e6e6;
 +
}
 +
 +
.pricing-box.activeItem .pricing-offer{
 +
color:#fff;
 +
}
 +
 +
.pricing-offer strong{
 +
font-size:78px;
 +
line-height:89px;
 +
}
 +
 +
.pricing-offer sup{
 +
font-size:28px;
 +
}
 +
 +
.pricing-container{
 +
background: #fff;
 +
text-align:center;
 +
font-size:14px;
 +
}
 +
 +
.pricing-container strong{
 +
color:#353535;
 +
}
 +
 +
.pricing-container ul{
 +
list-style:none;
 +
padding:0;
 +
margin:0;
 +
}
 +
 +
.pricing-container ul li{
 +
border-bottom: 1px solid #CFCFCF;
 +
list-style: none;
 +
padding: 15px 0 15px 0;
 +
margin: 0 0 0 0;
 +
color: #222;
 +
}
 +
 +
.pricing-action{
 +
margin:0;
 +
background: #fcfcfc;
 +
text-align:center;
 +
padding:20px 0 30px 0;
 +
}
 +
 +
.pricing-wrapp{
 +
margin:0 auto;
 +
width:100%;
 +
background:#fd0000;
 +
}
 +
.pricing-box-item {border: 1px solid #e6e6e6;background:#fcfcfc;position:relative;margin:0 0 20px 0;padding:0;-webkit-box-shadow: 0 2px 0 rgba(0,0,0,0.03);-moz-box-shadow: 0 2px 0 rgba(0,0,0,0.03);box-shadow: 0 2px 0 rgba(0,0,0,0.03);-webkit-box-sizing: border-box;-moz-box-sizing: border-box;box-sizing: border-box;overflow: hidden;}
 +
 +
.pricing-box-item .pricing-heading {
 +
background: #ffffff;
 +
text-align: center;
 +
padding: 50px 0;
 +
display:block;
 +
}
 +
.pricing-box-item.activeItem .pricing-heading {
 +
/* background: #f3f3f3; */
 +
text-align: center;
 +
border-bottom:none;
 +
display:block;
 +
color:#fff;
 +
}
 +
.pricing-box-item.activeItem .pricing-heading h3 {
 +
 +
color: #fff;
 +
}
 +
 +
.pricing-box-item .pricing-heading h3 strong {
 +
font-size:28px;
 +
font-weight:700;
 +
letter-spacing:-1px;
 +
color: #05142b;
 +
}
 +
.pricing-box-item .pricing-heading h3 {
 +
font-size:32px;
 +
font-weight:300;
 +
letter-spacing:-1px;
 +
}
 +
 +
.pricing-box-item .pricing-terms {
 +
text-align: center;
 +
background: #fff;
 +
display: block;
 +
overflow: hidden;
 +
padding: 11px 0 5px;
 +
/* border-top: 2px solid #BBBBBB; */
 +
border-bottom: 2px dotted #CDCDCD;
 +
}
 +
 +
.pricing-box-item .pricing-terms  h6 {
 +
margin-top: 16px;
 +
color: #ff5722;
 +
font-size: 22px;
 +
}
 +
 +
.pricing-box-item .icon .price-circled {
 +
    margin: 10px 10px 10px 0;
 +
    display: inline-block !important;
 +
    text-align: center !important;
 +
    color: #fff;
 +
    width: 68px;
 +
    height: 68px;
 +
padding:12px;
 +
    font-size: 16px;
 +
font-weight:700;
 +
    line-height: 68px;
 +
    text-shadow:none;
 +
    cursor: pointer;
 +
    background-color: #888;
 +
    border-radius: 64px;
 +
    -moz-border-radius: 64px;
 +
    -webkit-border-radius: 64px;
 +
}
 +
 +
.pricing-box-item  .pricing-action{
 +
margin:0;
 +
text-align:center;
 +
padding:30px 0 30px 0;
 +
}
 +
.pricing-action .btn{
 +
background: #ff5722;
 +
color: #fff;
 +
}
 +
.menuItem .menu{font-size:20px;font-weight:600;list-style:none;padding:0 0 15px;margin-bottom:20px;display:inline-block;color: #e91e63;}
 +
.menuItem .menu li{padding: 16px 30px 0px;margin-bottom: 0;display:inline-block;width:100%;float:left;background: #ffffff;line-height: 30px;border-left: 2px solid #e91e63;border-radius: 0;}
 +
.menuItem .detail{
 +
font-size:13px;
 +
color:#444;
 +
font-weight:normal;
 +
}
 +
.menuItem .price{
 +
font-size:13px;
 +
float:right;
 +
font-weight:600;
 +
}
 +
/* Testimonials */
 +
.testimonials blockquote {
 +
    background: #ffffff none repeat scroll 0 0;
 +
    border: medium none;
 +
    color: #666;
 +
    display: block;
 +
    font-size: 13px;
 +
    line-height: 20px;
 +
    padding: 15px;
 +
    position: relative;
 +
}
 +
.testimonials blockquote::before {
 +
    width: 0;
 +
    height: 0;
 +
right: 0;
 +
bottom: 0;
 +
content: " ";
 +
display: block;
 +
position: absolute;
 +
    border-bottom: 20px solid #fff;   
 +
border-right: 0 solid transparent;
 +
border-left: 15px solid transparent;
 +
border-left-style: inset; /*FF fixes*/
 +
border-bottom-style: inset; /*FF fixes*/
 +
}
 +
.testimonials blockquote::after {
 +
    width: 0;
 +
    height: 0;
 +
    right: 0;
 +
    bottom: 0;
 +
    content: " ";
 +
    display: block;
 +
    position: absolute;
 +
    border-style: solid;
 +
    border-width: 20px 20px 0 0;
 +
    border-color: #c5c5c5 transparent transparent transparent;
 +
}
 +
.testimonials .carousel-info img {
 +
    border: 1px solid #cccccc;
 +
    border-radius: 150px !important;
 +
    height: 75px;
 +
    padding: 3px;
 +
    width: 75px;
 +
}
 +
.testimonials .carousel-info {
 +
    overflow: hidden;
 +
}
 +
.testimonials .carousel-info img {
 +
    margin-right: 15px;
 +
}
 +
.testimonials .carousel-info span {
 +
    display: block;
 +
}
 +
.testimonials span.testimonials-name {
 +
    color: #e91e63;
 +
    font-size: 16px;
 +
    font-weight: 300;
 +
    margin: 23px 0 7px;
 +
}
 +
.testimonials span.testimonials-post {
 +
    color: #656565;
 +
    font-size: 12px;
 +
}
 +
/* ===== Widgets ===== */
 +
 +
/* --- flickr --- */
 +
.widget .flickr_badge {
 +
width:100%;
 +
}
 +
.widget .flickr_badge img { margin: 0 9px 20px 0; }
 +
 +
footer .widget .flickr_badge {
 +
    width: 100%;
 +
}
 +
footer .widget .flickr_badge img {
 +
    margin: 0 9px 20px 0;
 +
}
 +
 +
.flickr_badge img {
 +
    width: 50px;
 +
    height: 50px;
 +
    float: left;
 +
margin: 0 9px 20px 0;
 +
}
 +
 +
/* --- Recent post widget --- */
 +
 +
.recent-post{
 +
margin:20px 0 0 0;
 +
padding:0;
 +
line-height:18px;
 +
}
 +
 +
.recent-post h5 a:hover {
 +
text-decoration:none;
 +
}
 +
 +
.recent-post .text h5 a {
 +
color:#353535;
 +
}
 +
 +
 
 +
footer{
 +
padding:50px 0 0 0;
 +
color:#f8f8f8;
 +
}
 +
 +
footer a {
 +
color:#fff;
 +
}
 +
 +
footer a:hover {
 +
color: #e91e63;
 +
}
 +
 +
footer h1, footer h2, footer h3, footer h4, footer h5, footer h6{
 +
color: #d3d3d3;
 +
}
 +
 +
footer address {
 +
line-height:1.6em;
 +
color: #797979;
 +
}
 +
 +
footer h5 a:hover, footer a:hover {
 +
text-decoration:none;
 +
}
 +
 +
ul.social-network {
 +
list-style:none;
 +
margin: 4px 0 0 -25px;
 +
}
 +
 +
ul.social-network li {
 +
display:inline;
 +
margin: 0 5px;
 +
/* border: 1px solid #2D2D2D; */
 +
padding: 5px 0 0;
 +
width: 32px;
 +
display: inline-block;
 +
text-align: center;
 +
height: 32px;
 +
vertical-align: baseline;
 +
}
 +
 +
#sub-footer{
 +
text-shadow:none;
 +
color: #dedede;
 +
padding:0;
 +
padding-top:30px;
 +
margin:20px 0 0 0;
 +
background: #0a0a0a;
 +
border-top: 1px solid #3e3e3e;
 +
}
 +
 +
#sub-footer p{
 +
margin:0;
 +
padding:0;
 +
}
 +
 +
#sub-footer span{
 +
color: #9e9e9e;
 +
}
 +
 +
.copyright {
 +
text-align:left;
 +
font-size:12px;
 +
}
 +
 +
#sub-footer ul.social-network {
 +
float:right;
 +
}
 +
 +
 
 +
 +
/* scroll to top */
 +
.scrollup{
 +
    position:fixed;
 +
    width:32px;
 +
    height:32px;
 +
    bottom:0px;
 +
    right:20px;
 +
    background: #e91e63;
 +
}
 +
 +
a.scrollup {
 +
outline:0;
 +
text-align: center;
 +
}
 +
 +
a.scrollup:hover,a.scrollup:active,a.scrollup:focus {
 +
opacity:1;
 +
text-decoration:none;
 +
}
 +
a.scrollup i {
 +
margin-top: 10px;
 +
color: #fff;
 +
}
 +
a.scrollup i:hover {
 +
text-decoration:none;
 +
}
 +
 +
 +
 +
 +
.absolute{
 +
position:absolute;
 +
}
 +
 +
.relative{
 +
position:relative;
 +
}
 +
 +
.aligncenter{
 +
text-align:center;
 +
}
 +
 +
.aligncenter span{
 +
margin-left:0;
 +
}
 +
 +
.floatright {
 +
float:right;
 +
}
 +
 +
.floatleft {
 +
float:left;
 +
}
 +
 +
.floatnone {
 +
float:none;
 +
}
 +
 +
.aligncenter {
 +
text-align:center;
 +
}
 +
 +
img.pull-left, .align-left{
 +
float:left;
 +
margin:0 15px 15px 0;
 +
}
 +
 +
.widget img.pull-left {
 +
float:left;
 +
margin:0 15px 15px 0;
 +
}
 +
 +
img.pull-right, .align-right {
 +
float:right;
 +
margin:0 0 15px 15px;
 +
}
 +
 +
article img.pull-left, article .align-left{
 +
float:left;
 +
margin:5px 15px 15px 0;
 +
}
 +
 +
article img.pull-right, article .align-right{
 +
float:right;
 +
margin:5px 0 15px 15px;
 +
}
 +
============================= */
 +
 +
.clear-marginbot{
 +
margin-bottom:0;
 +
}
 +
 +
.marginbot10{
 +
margin-bottom:10px;
 +
}
 +
.marginbot20{
 +
margin-bottom:20px;
 +
}
 +
.marginbot30{
 +
margin-bottom:30px;
 +
}
 +
.marginbot40{
 +
margin-bottom:40px;
 +
}
 +
 +
.clear-margintop{
 +
margin-top:0;
 +
}
 +
 +
.margintop10{
 +
margin-top:10px;
 +
}
 +
 +
.margintop20{
 +
margin-top:20px;
 +
}
 +
 +
.margintop30{
 +
margin-top:30px;
 +
}
 +
 +
.margintop40{
 +
margin-top:40px;
 +
}
 +
.flex-control-paging li a {
 +
  width: 14px;
 +
  height: 14px;
 +
  border-radius: 50%;
 +
  background: #ffffff;
 +
  text-indent: 50px;
 +
  overflow: hidden;
 +
}
 +
.btn:hover, .btn:focus {
 +
  color: #FFF;
 +
  text-decoration: none;
 +
  background: #d41a59;
 +
  border-color: #d41a59;
 +
}
 +
/*  Media queries
 +
============================= */
 +
 +
@media (min-width: 768px) and (max-width: 979px) {
 +
 +
a.detail{
 +
background:none;
 +
width:100%;
 +
}
 +
 +
header  .navbar-nav > li > a{
 +
 +
}
 +
 +
footer .widget form  input#appendedInputButton {
 +
  display: block;
 +
  width: 91%;
 +
  -webkit-border-radius: 4px 4px 4px 4px;
 +
-moz-border-radius: 4px 4px 4px 4px;
 +
  border-radius: 4px 4px 4px 4px;
 +
}
 +
 +
footer .widget form  .input-append .btn {
 +
  display: block;
 +
  width: 100%;
 +
  padding-right: 0;
 +
  padding-left: 0;
 +
  -webkit-box-sizing: border-box;
 +
-moz-box-sizing: border-box;
 +
  box-sizing: border-box;
 +
  margin-top:10px;
 +
}
 +
 +
ul.related-folio li{
 +
width:156px;
 +
margin:0 20px 0 0;
 +
}
 +
}
 +
 +
@media (max-width: 767px) {
 +
.navbar-default .navbar-collapse{border-color: none;/* background: #05142b; */}
 +
.navbar-default .navbar-toggle .icon-bar {background-color: #868686;border-radius: 0;}
 +
.navbar-default .navbar-toggle:hover, .navbar-default .navbar-toggle:focus {
 +
background-color: rgba(221, 221, 221, 0);
 +
}
 +
.navbar-default .navbar-collapse, .navbar-default .navbar-form {
 +
border-color: rgba(255, 255, 255, 0.58);
 +
margin-top: 15px;
 +
}
 +
.flex-caption h3{
 +
font-size:28px;
 +
}
 +
  body {
 +
    padding-right: 0;
 +
    padding-left: 0;
 +
  }
 +
.navbar-brand {
 +
margin-top: 18px;
 +
border-bottom: none;
 +
}
 +
.navbar-header {
 +
/* margin-top: 20px; */
 +
border-bottom: none;
 +
}
 +
 +
.navbar-nav {
 +
border-top: none;
 +
float: none;
 +
width: 100%;
 +
}
 +
.navbar .nav > .active > a, .navbar .nav > .active > a:hover {background: none;font-weight: 700;color: #10cdff;}
 +
header .navbar-nav > li {padding-bottom: 0;padding-top: 0;}
 +
header .nav li .dropdown-menu  {
 +
margin-top: 0;
 +
}
 +
header  .navbar-nav > li > a{
 +
line-height: 20px;
 +
}
 +
.dropdown-menu {
 +
  position: absolute;
 +
  top: 0;
 +
  left: 40px;
 +
  z-index: 1000;
 +
  display: none;
 +
  float: left;
 +
  min-width: 160px;
 +
  padding: 5px 0;
 +
  margin: 2px 0 0;
 +
  font-size: 13px;
 +
  list-style: none;
 +
  background-color: #fff;
 +
  background-clip: padding-box;
 +
  border: 1px solid #f5f5f5;
 +
  border: 1px solid rgba(0, 0, 0, .15);
 +
  border-radius: 0;
 +
  -webkit-box-shadow: 0 6px 12px rgba(0, 0, 0, .175);
 +
  box-shadow: 0 6px 12px rgba(0, 0, 0, .175);
 +
}
 +
 +
 +
 +
li.active  {
 +
border: none;
 +
overflow: hidden;
 +
/* background: #2e8eda; */
 +
}
 +
 +
 +
.box {
 +
border-bottom:1px solid #e9e9e9;
 +
padding-bottom:20px;
 +
}
 +
 +
.flexslider .slide-caption {
 +
width: 90%;
 +
padding: 2%;
 +
position: absolute;
 +
left: 0;
 +
bottom: -40px;
 +
}
 +
 +
 +
#inner-headline .breadcrumb {
 +
float:left;
 +
clear:both;
 +
width:100%;
 +
}
 +
 +
.breadcrumb > li {
 +
font-size:13px;
 +
}
 +
 +
 +
ul.portfolio li article a i.icon-48{
 +
width:20px;
 +
height:20px;
 +
font-size:16px;
 +
line-height:20px;
 +
}
 +
 +
 +
.left-sidebar{
 +
border-right:none;
 +
padding:0 0 0 0;
 +
border-bottom: 1px dotted #e6e6e6;
 +
padding-bottom:10px;
 +
margin-bottom:40px;
 +
}
 +
 +
.right-sidebar{
 +
margin-top:30px;
 +
border-left:none;
 +
padding:0 0 0 0;
 +
}
 +
 +
 +
footer .col-lg-1, footer .col-lg-2, footer .col-lg-3, footer .col-lg-4, footer .col-lg-5, footer .col-lg-6,
 +
footer .col-lg-7, footer .col-lg-8, footer .col-lg-9, footer .col-lg-10, footer .col-lg-11, footer .col-lg-12{
 +
margin-bottom:20px;
 +
}
 +
 +
#sub-footer ul.social-network {
 +
float:left;
 +
}
 +
 +
 +
 +
  [class*="span"] {
 +
margin-bottom:20px;
 +
  }
 +
 +
}
 +
 +
@media (max-width: 480px) {
 +
.bottom-article a.pull-right {
 +
float:left;
 +
margin-top:20px;
 +
}
 +
 +
 +
.search{
 +
float:left;
 +
}
 +
 +
.flexslider .flex-caption {
 +
display:none;
 +
}
 +
 +
 +
.cta-text {
 +
margin:0 auto;
 +
text-align:center;
 +
}
 +
 +
ul.portfolio li article a i{
 +
width:20px;
 +
height:20px;
 +
font-size:14px;
 +
}
 +
 +
 +
}
 +
.tab-section .col{
 +
 +
}
 +
.tab-section .container{
 +
padding: 15px;
 +
border: 1px solid #eaeaea;
 +
background: #fff;
 +
}
 +
.active .collapsible-header{color: #e91e63;}
 +
span.color {
 +
    color: #e91e63;
 +
    text-transform: uppercase;
 +
}
 +
.input-field label {
 +
    color: #888;
 +
font-size:14px;
 +
}
 +
.input-field {
 +
    position: relative;
 +
    margin-top: 1rem;
 +
    float: left;
 +
    width: 100%;
 +
}
 +
.alert-danger {
 +
    float: left;
 +
}
 +
 +
 +
/**********************
 +
  ****iGEM*************************************************************
 +
**************/
 +
<script>
 +
    if ($('.firstHeading')) $('.firstHeading').remove();
 +
    </script>
 +
    <style>
 +
    html {
 +
        overflow-x: hidden;
 +
        width: 100%;
 +
    }
 +
   
 +
    .banner-top {
 +
        margin-left: 15%;
 +
    }
 +
   
 +
    #igem_home_logo {
 +
        width: 100%;
 +
        margin-top: 80px;
 +
    }
 +
   
 +
    @media (min-width: 992px) {
 +
        .work-content .col-md-4 {
 +
            width: 25%;
 +
            margin-right: 8%;
 +
        }
 +
    }
 +
   
 +
    .carousel-control.left,
 +
    .carousel-control.right {
 +
        background-image: none;
 +
        padding-top: 18%;
 +
    }
 +
   
 +
    .carousel-indicators li {
 +
        background-color: #fff;
 +
        border: 1px solid #a94442;
 +
    }
 +
   
 +
    .carousel-indicators li.active {
 +
        background-color: #f0ad4e;
 +
    }
 +
   
 +
    #contentSub,
 +
    #contentcontainer,
 +
    #footer-box,
 +
    #sitesub,
 +
    #catlinks,
 +
    #search-controls,
 +
    #p-logo,
 +
    .h3,
 +
    .printfooter,
 +
    .firstHeading,
 +
    .visualClear {
 +
        display: none;
 +
        box-sizing: content-box;
 +
    }
 +
   
 +
    #sideMenu,
 +
    #top_title {
 +
        display: none;
 +
        box-sizing: content-box;
 +
    }
 +
   
 +
    #sideMenu .mainMenu_toggle,
 +
    #sideMenu .mainMenu_Title,
 +
    #sideMenu .sideMenuTitle,
 +
    #sideMenu .expand_subMenus {
 +
        box-sizing: content-box;
 +
    }
 +
   
 +
    #bodyContent h1,
 +
    #bodyContent h2,
 +
    #bodyContent h3,
 +
    #bodyContent h4,
 +
    #bodyContent h5 {
 +
        margin-bottom: 0px;
 +
    }
 +
   
 +
    #search_text {
 +
        color: #000;
 +
    }
 +
   
 +
    #globalWrapper,
 +
    #content {
 +
        position: absolute;
 +
        left: 0;
 +
        right: 0;
 +
        top: 0;
 +
        width: 100%;
 +
        height: 100%;
 +
        border: 0px;
 +
        background-color: transparent;
 +
        margin: 0px;
 +
        padding: 0px;
 +
    }
 +
   
 +
    @media (min-width: 1200px) {
 +
        .container-fluid .container {
 +
            width: 969px;
 +
            /* margin-left: 300px; */
 +
        }
 +
        .head-container {
 +
            width: 1170px;
 +
        }
 +
    }
 +
    /* article */
 +
   
 +
    .text-content article p {
 +
        text-indent: 20px;
 +
        margin: 10px 0;
 +
        font-size: 15px;
 +
        line-height: 24px;
 +
    }
 +
   
 +
    .text-content article img {
 +
        width: 80%;
 +
        padding-left: 20%;
 +
        /* margin-left: 20%; */
 +
    }
 +
    /*    .mw-content-ltr ol{
 +
        margin:0;
 +
    } */
 +
   
 +
    ol.carousel-indicators {
 +
        position: absolute;
 +
        bottom: 10px;
 +
        left: 50%;
 +
        z-index: 15;
 +
        width: 60%;
 +
        padding-left: 0;
 +
        margin-left: -30%;
 +
        text-align: center;
 +
        list-style: none;
 +
    }
 +
 +
       
 +
        @charset "utf-8";
 +
    body {
 +
        font-family: "Helvetica Neue", Helvetica, Arial, "Microsoft Yahei UI", "Microsoft YaHei", SimHei, "\5B8B\4F53", simsun, sans-serif;
 +
        color: #666;
 +
        width:100%;
 +
    }
 +
    .logo {
 +
        padding:0;
 +
    }
 +
 +
    #myCarousel {
 +
        margin: 50px 0 0 0;
 +
    }
 +
    #navbar-collapse ul {
 +
        margin-top:0;
 +
    }
 +
    .carousel-inner img {
 +
        margin: 0 auto;
 +
    }
 +
    /*.carousel-control {
 +
        font-size: 100px;
 +
    }*/
 +
    .tab-h1 {
 +
        width:100%;
 +
        font-size: 30px;
 +
        text-align: center;
 +
        letter-spacing: 1px;
 +
        color: #ffffff;
 +
        background-color:#97477c;
 +
        padding: 30px;
 +
        margin: 0;
 +
    }
 +
 +
    .tab-p {
 +
        font-size: 20px;
 +
        text-align: center;
 +
        letter-spacing: 1px;
 +
        color: #999;
 +
        margin: 20px 0 40px 0;
 +
    }
 +
 +
    .container-fluid{
 +
        width:100%;
 +
    }
 +
 +
    .tab1 {
 +
        width:100%;
 +
        margin: 0 0;
 +
        color: #666;
 +
        background:#333;
 +
    }
 +
 +
    .tab1 .text-muted {
 +
        color: #999;
 +
        text-decoration: line-through;
 +
    }
 +
    .tab1 .media-heading {
 +
        margin: 5px 0 20px 0;
 +
    }
 +
    .tab1 .col {
 +
        padding: 20px;
 +
    }
 +
 +
    .text span{
 +
        color: #97477c;
 +
    }
 +
    .text p {
 +
        font-size: 14px;
 +
    }
 +
    #footer {
 +
        background-color: #eee;
 +
        border-top: 1px solid #ccc;
 +
        padding: 20px;
 +
        text-align: center;
 +
    }
 +
    .text h4{
 +
        padding: 5px 10px;
 +
    }
 +
    .text p{
 +
        padding: 2px 10px;
 +
    }
 +
    .text h5{
 +
        padding: 3px 10px;
 +
    }
 +
    .dropdown button{
 +
        margin: 2px 20px;
 +
    }
 +
    /*没有小于768px,是因为Bootstrap3以移动端优先设计*/
 +
 +
    /* 小屏幕(平板,大于等于768px) */
 +
    @media (min-width: 768px) {
 +
        .tab-h1 {
 +
            font-size: 26px;
 +
        }
 +
        .tab-p {
 +
            font-size: 16px;
 +
        }
 +
        .text h3 {
 +
            font-size: 22px;
 +
        }
 +
        .text p {
 +
            font-size: 10px;
 +
            text-indent: 20px;
 +
        }
 +
        .tab2-text {
 +
            float: left;
 +
        }
 +
        .tab2-img {
 +
            float: right;
 +
        }
 +
    }
 +
 +
    /* 中等屏幕(桌面显示器,大于等于992px) */
 +
    @media (min-width: 992px) {
 +
        .tab-h1 {
 +
            font-size: 28px;
 +
        }
 +
        .tab-p {
 +
            font-size: 17px;
 +
        }
 +
        .text h3 {
 +
            font-size: 24px;
 +
        }
 +
        .text p {
 +
            font-size: 12px;
 +
            text-indent: 24px;
 +
        }
 +
    }
 +
 +
    /* 大屏幕(大桌面显示器,大于等于1200px) */
 +
    @media (min-width: 1200px) {
 +
        .tab-h1 {
 +
            font-size: 30px;
 +
        }
 +
        .tab-p {
 +
            font-size: 18px;
 +
        }
 +
        .text h3 {
 +
            font-size: 26px;
 +
        }
 +
        .text p {
 +
            font-size: 14px;
 +
            text-indent: 28px;
 +
        }
 +
    }
 +
       
 +
 +
    body {
 +
            position: relative;
 +
        }
 +
        ul.nav-pills {
 +
            top: 200px;
 +
            position: fixed;
 +
        }
 +
        ul.nav-pills2 {
 +
            bottom:50px;
 +
            position: fixed;
 +
        }
 +
        div.col-sm-10 div {
 +
            min-height: 10px;
 +
            font-size: 14px;
 +
            text-indent: 28px;
 +
            color:#0f0f0f;
 +
        }
 +
 +
        @media screen and (max-width: 992px) {
 +
            #section1, #section2, #section3, #section4, #section5 {
 +
            background-color: #eee; color:#171717;
 +
            }
 +
        }
 +
 +
        body {
 +
                position: relative;
 +
            }
 +
        .menu_logo_1 {
 +
                top: 18px;
 +
                left:80px;
 +
                z-index: -1000;
 +
                opacity: 0.8;
 +
                position: fixed;
 +
        }
 +
        .menu_logo_2 {
 +
                top: 18px;
 +
                left:170px;
 +
                z-index: -1000;
 +
                opacity: 0.8;
 +
                position: fixed;
 +
        }
 +
    </style>
 +
 +
 +
 +
 +
 +
</style>
 +
 +
<!-- HTML5 shim, for IE6-8 support of HTML5 elements -->
 +
<!--[if lt IE 9]>
 +
      <script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script>
 +
    <![endif]-->
 +
<link rel="stylesheet" href="https://2018.igem.org/Team:HUST-China/css/menu-slider?action=raw&amp;ctype=text/css" type="text/css" />
 +
<script type="text/javascript" src="https://2018.igem.org/Team:HUST-China/js/slider-jquery?action=raw&amp;ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2018.igem.org/Team:HUST-China/js/slider-jquery-flexslider-min?action=raw&amp;ctype=text/javascript"></script>
 +
</head>
 +
<body>
 +
<div id="wrapper" class="home-page">
 +
<!-- start header -->
 +
<header style="margin-top: 12px">
 +
        <div class="navbar navbar-default navbar-static-top">
 +
            <div class="container">
 +
                <div class="navbar-header">
 +
                    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse">
 +
                        <span class="icon-bar"></span>
 +
                        <span class="icon-bar"></span>
 +
                        <span class="icon-bar"></span>
 +
                    </button>
 +
                    <a class="navbar-brand" href="index.html"><i class="icon-info-blocks material-icons">account_balance</i>2018-HUST-China</a>
 +
                </div>
 +
                <div class="navbar-collapse collapse ">
 +
                    <ul class="nav navbar-nav">
 +
                      <li class="HillSide"><a class="waves-effect waves-dark" href="index.html">Home</a></li>
 +
          <li class="dropdown">
 +
                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">PROJECT<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu">
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/project/background">Background</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Description">Description</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Safety">Safety</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Demonstrate">Demonstrate</a></li>
 +
                          </ul>
 +
                      </li>
 +
                      <li class="dropdown">
 +
                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">WETLAB<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu">
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Experiments">Experiments</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Results">Results</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/InterLab">Interlab</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Notebook">Notebook</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/wetlab/protocols">Protocols</a></li>
 +
                          </ul>
 +
                      </li>
 +
          <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Model">MODEL</a></li>
 +
                      <li class="dropdown">
 +
                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">PARTS<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu">
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Basic part">Basic part</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Composite part">Composite part</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Part collection">Part collection</a></li>
 +
                          </ul>
 +
                      </li>
 +
                      <li class="dropdown">
 +
                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">HP<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu">
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/HP/Silver">HP/Silver</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Engagement">Engagement</a></li>
 +
                          </ul>
 +
                      </li>
 +
                      <li class="dropdown">
 +
                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">TEAM<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu">
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Team">Team Roster</a></li>
 +
                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/HP/Silver">Attributions</a></li>
 +
                          </ul>
 +
                      </li>
 +
                    </ul>
 +
                </div>
 +
            </div>
 +
        </div>
 +
</header>
 +
<!-- end header -->
 +
 +
          <section id="inner-headline">
 +
            <div class="container">
 +
              <div class="row">
 +
                <div class="col-lg-12">
 +
                  <h2 class="pageTitle">Discription</h2>
 +
                </div>
 +
              </div>
 +
            </div>
 +
          </section>
 +
 +
    <div class="container">
 +
      <div class="row">
 +
       
 +
        <div class="col-md-6">
 +
          <div class="about-text">
 +
            <h3><span class="color">Shewanella</span></h3>
 +
            <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
 +
            <p>In the part of Shewanella, we employ Shewanella oneidensis MR-1 as our chassis and pyydt as the plasmid. Shewanella could utilize the lactate as its carbon source and produce electricity with the help of a multiprotein porin-cytochrome complex on its outer membrane called MtrABC. MtrABC could transfer the electron from Shewanella’s inner membrane to the surrounding. pYYDT is a shuttle plasmid between E.Coli and Shewanella. We constructed this plasmid in E.Coli and transferred it into Shewanella by conjugating these two kinds of bacteria.</p>
 +
            <div class="col-md-6">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b6/T--HUST-China--2018-description-logo1.png" alt="showcase image">
 +
        </div>
 +
           
 +
            <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
       
 +
            <p>Our design of constructing Shewanella is devided into two parts: improvement of utilization of lactate and increment of the amount of NADH in Shewanella.</p>
 +
            <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
            <p>(1). Improvement of utilization of lactate:
 +
To make the use of lactate more efficiently, we overexpress four genes: dld-Ⅱ, lldE, lldF, lldG.</p>
 +
          </div>
 +
          <p>①. dld-Ⅱ: dld-Ⅱ refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. </p>
 +
          <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
            <p>②. lldE/lldF/lldG: They could encode a L-lactate dehydrogenase complex which could catalyze D-lactate’s transformation into pyruvate.
 +
We constructed them separately in pYYDT and found out if there was any improvement at the amount of electricty produced by our Shewanella.</p>
 +
<div class="col-md-6">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/eb/T--HUST-China--2018-description-logo2.png" alt="showcase image">
 +
         
 +
        </div>
 +
        <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
<div class="col-md-6">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/6/66/T--HUST-China--2018-description-logo3.png" alt="showcase image">
 +
        </div>
 +
        <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
         
 +
        <p>Then we put these four genes in one circuit to level up the production of electricity.</p>
 +
        <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
        <div class="col-md-6">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/4b/T--HUST-China--2018-description-logo4.png" alt="showcase image">
 +
        </div>
 +
        <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
        <p>(2). Increment of the amount of NADH in Shewanella</p>
 +
        <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
            <p>To achieve this goal, we overexpress these four genes: gapA2, mdh, pflB, fdh*. We chose them according to our reference.</p>
 +
            <p>①. gapA2: It encodes glyceraldehyde-3-phosphate dehydrogenase which could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate</p>
 +
            <p>②. mdh: It encodes NAD dependent malate dehydrogenase which transforms malate into pyruvate</p>
 +
              <p>③. pflB: It encodes pyruvate formate-lyase to transform pyruvate into Acetyl-CoA</p>
 +
              <p>③. pflB: It encodes pyruvate formate-lyase to transform pyruvate into Acetyl-CoA</p>
 +
              <div class="col-md-6">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/26/T--HUST-China--2018-description-logo5.png" alt="showcase image">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b7/T--HUST-China--2018-description-logo6.png" alt="showcase image">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/f/f0/T--HUST-China--2018-description-logo7.png" alt="showcase image">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/2a/T--HUST-China--2018-description-logo8.png" alt="showcase image">
 +
        </div>
 +
        <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
        <p>According to our model, we divided these four genes into two groups: gapA2/mdh and pflB/fdh*. </p>
 +
        <div class="col-md-6">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/23/T--HUST-China--2018-description-logo9.png" alt="showcase image">
 +
          <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/47/T--HUST-China--2018-description-logo10.png" alt="showcase image">
 +
        </div>
 +
        <div class="container">
 +
              <div class="row">
 +
               
 +
              </div>
 +
            </div>
 +
        <p>Finally, we put all these genes into one circuit and found out if the amount of electricity could reach the maximum. </p>
 +
        <img  class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/82/T--HUST-China--2018-description-logo11.png" alt="showcase image">
 +
 +
 +
 +
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </section>
 +
</section>  
 +
 +
 +
<footer>
 +
<div class="container">
 +
<div class="row">
 +
<div class="col-sm-3">
 +
<div class="widget">
 +
<h5 class="widgetheading">Our Contact</h5>
 +
<address>
 +
<strong>Bootstrap company Inc</strong><br>
 +
JC Main Road, Near Silnile tower<br>
 +
Pin-21542 NewYork US.</address>
 +
<p>
 +
<i class="icon-phone"></i> (123) 456-789 - 1255-12584 <br>
 +
<i class="icon-envelope-alt"></i> email@domainname.com
 +
</p>
 +
</div>
 +
</div>
 +
<div class="col-sm-3">
 +
<div class="widget">
 +
<h5 class="widgetheading">Quick Links</h5>
 +
<ul class="link-list">
 +
<li><a class="waves-effect waves-dark" href="#">Latest Events</a></li>
 +
<li><a class="waves-effect waves-dark" href="#">Terms and conditions</a></li>
 +
<li><a class="waves-effect waves-dark" href="#">Privacy policy</a></li>
 +
<li><a class="waves-effect waves-dark" href="#">Career</a></li>
 +
<li><a class="waves-effect waves-dark" href="#">Contact us</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
<div class="col-sm-3">
 +
<div class="widget">
 +
<h5 class="widgetheading">Latest posts</h5>
 +
<ul class="link-list">
 +
<li><a class="waves-effect waves-dark" href="#">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</a></li>
 +
<li><a class="waves-effect waves-dark" href="#">Pellentesque et pulvinar enim. Quisque at tempor ligula</a></li>
 +
<li><a class="waves-effect waves-dark" href="#">Natus error sit voluptatem accusantium doloremque</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
<div class="col-sm-3">
 +
<div class="widget">
 +
<h5 class="widgetheading">Recent News</h5>
 +
<ul class="link-list">
 +
<li><a class="waves-effect waves-dark" href="#">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</a></li>
 +
<li><a class="waves-effect waves-dark" href="#">Pellentesque et pulvinar enim. Quisque at tempor ligula</a></li>
 +
<li><a class="waves-effect waves-dark" href="#">Natus error sit voluptatem accusantium doloremque</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<section id="banner">
 +
 +
</section> 
 +
<div id="sub-footer">
 +
<div class="container">
 +
<div class="row">
 +
<div class="col-lg-6">
 +
<div class="copyright">
 +
<p>
 +
<span>&copy; Bootstrap Template 2018 All right reserved. Template By </span><a href="https://webthemez.com/responsive-website/" target="_blank">WebThemez</a>
 +
</p>
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
</footer>
 +
</div>
 +
<!-- javascript
 +
    ================================================== -->
 +
<!-- Placed at the end of the document so the pages load faster -->
 +
<script src="https://2018.igem.org/Team:HUST-China/js/jquery?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2018.igem.org/Team:HUST-China/js/jquery-easing-1-3?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2018.igem.org/Team:HUST-China/materialize/js/materialize-min?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2018.igem.org/Team:HUST-China/js/bootstrap-min?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2018.igem.org/Team:HUST-China/js/jquery-fancybox-pack?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2018.igem.org/Team:HUST-China/js/jquery-fancybox-media?action=raw&amp;ctype=text/javascript"></script> 
 +
<script src="https://2018.igem.org/Team:HUST-China/js/jquery-flexslider?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2018.igem.org/Team:HUST-China/js/animate?action=raw&amp;ctype=text/javascript"></script>
 +
<!-- Vendor Scripts -->
 +
<script src="https://2018.igem.org/Team:HUST-China/js/modernizr-custom?action=raw&amp;ctype=text/javascript"></script>
 +
<!--
 +
<script src="js/modernizr.custom.js"></script>
 +
-->
 +
<script src="js/jquery.zoomslider.min.js"></script>
 +
<script src="js/jquery.isotope.min.js"></script>
 +
<script src="js/jquery.magnific-popup.min.js"></script>
 +
<script src="js/animate.js"></script>
 +
<script src="js/custom.js"></script>
 +
<script type="text/javascript">
 +
 +
</script>
 +
</body>
 
</html>
 
</html>

Revision as of 13:30, 16 September 2018

HillSide Multi purpose HTML5 Template

Discription

Shewanella

In the part of Shewanella, we employ Shewanella oneidensis MR-1 as our chassis and pyydt as the plasmid. Shewanella could utilize the lactate as its carbon source and produce electricity with the help of a multiprotein porin-cytochrome complex on its outer membrane called MtrABC. MtrABC could transfer the electron from Shewanella’s inner membrane to the surrounding. pYYDT is a shuttle plasmid between E.Coli and Shewanella. We constructed this plasmid in E.Coli and transferred it into Shewanella by conjugating these two kinds of bacteria.

showcase image

Our design of constructing Shewanella is devided into two parts: improvement of utilization of lactate and increment of the amount of NADH in Shewanella.

(1). Improvement of utilization of lactate: To make the use of lactate more efficiently, we overexpress four genes: dld-Ⅱ, lldE, lldF, lldG.

①. dld-Ⅱ: dld-Ⅱ refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate.

②. lldE/lldF/lldG: They could encode a L-lactate dehydrogenase complex which could catalyze D-lactate’s transformation into pyruvate. We constructed them separately in pYYDT and found out if there was any improvement at the amount of electricty produced by our Shewanella.

showcase image
showcase image

Then we put these four genes in one circuit to level up the production of electricity.

showcase image

(2). Increment of the amount of NADH in Shewanella

To achieve this goal, we overexpress these four genes: gapA2, mdh, pflB, fdh*. We chose them according to our reference.

①. gapA2: It encodes glyceraldehyde-3-phosphate dehydrogenase which could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate

②. mdh: It encodes NAD dependent malate dehydrogenase which transforms malate into pyruvate

③. pflB: It encodes pyruvate formate-lyase to transform pyruvate into Acetyl-CoA

③. pflB: It encodes pyruvate formate-lyase to transform pyruvate into Acetyl-CoA

showcase image showcase image showcase image showcase image

According to our model, we divided these four genes into two groups: gapA2/mdh and pflB/fdh*.

showcase image showcase image

Finally, we put all these genes into one circuit and found out if the amount of electricity could reach the maximum.

showcase image