Difference between revisions of "Team:Tacoma RAINmakers/Safety"
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<ul> | <ul> | ||
<li>Choosing a non-pathogenic chassis</li> | <li>Choosing a non-pathogenic chassis</li> | ||
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| + | RAINmakers are employing 5-alpha competent E. coli cells (DH5-Alpha strain). According to the Globally Harmonized Systems (GHS), this organism is not a dangerous substance or mixture. and this organism can be handled at Biology Safety Level One (BSL-1). The CDC goes on to state that 5-alpha competent E. coli cells present no risk of disease in immunocompetent adult humans and no hazard to laboratory personnel and the environment. There are certain first aid measures that must be taken if the 5-alpha E. coli cells come in contact with lab personnel. If the cells come in contact with skin, washing with soap and water is sufficient. If there is eye contact, rising thoroughly with water for 15 minutes is imperative, as well as consultation of a physician. In the case of inhalation, exposure to fresh air is all that is necessary. All members of Tacoma RAINmakers are versed in these simple first aid measures. In the case of accidental release into the environment, cells should be soaked up with inert absorbent material, transferred to a properly labelled container, and disposed of as hazardous waste. Still, it is important to recall that this organism presents no environmental threat. | ||
| + | </li> | ||
| + | <li>Since pure arsenic powder and solution are significantly toxic, we used the derivatives of arsenic, sodium arsenate and sodium | ||
| + | arsenite that are less dangerous than pure arsenic.</li> | ||
<li>Choosing parts that will not harm humans / animals / plants</li> | <li>Choosing parts that will not harm humans / animals / plants</li> | ||
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li> | <li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li> | ||
<li>Including an "induced lethality" or "kill-switch" device</li> | <li>Including an "induced lethality" or "kill-switch" device</li> | ||
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</ul> | </ul> | ||
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p> | <p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p> | ||
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<h3>Safe Shipment</h3> | <h3>Safe Shipment</h3> | ||
Revision as of 21:28, 16 September 2018
Team:ECUST/Lab/Notebook
Safety
Please visit the Safety Hub to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.
On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)
Safe Project Design
Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:
- Choosing a non-pathogenic chassis
- RAINmakers are employing 5-alpha competent E. coli cells (DH5-Alpha strain). According to the Globally Harmonized Systems (GHS), this organism is not a dangerous substance or mixture. and this organism can be handled at Biology Safety Level One (BSL-1). The CDC goes on to state that 5-alpha competent E. coli cells present no risk of disease in immunocompetent adult humans and no hazard to laboratory personnel and the environment. There are certain first aid measures that must be taken if the 5-alpha E. coli cells come in contact with lab personnel. If the cells come in contact with skin, washing with soap and water is sufficient. If there is eye contact, rising thoroughly with water for 15 minutes is imperative, as well as consultation of a physician. In the case of inhalation, exposure to fresh air is all that is necessary. All members of Tacoma RAINmakers are versed in these simple first aid measures. In the case of accidental release into the environment, cells should be soaked up with inert absorbent material, transferred to a properly labelled container, and disposed of as hazardous waste. Still, it is important to recall that this organism presents no environmental threat.
- Since pure arsenic powder and solution are significantly toxic, we used the derivatives of arsenic, sodium arsenate and sodium arsenite that are less dangerous than pure arsenic.
- Choosing parts that will not harm humans / animals / plants
- Substituting safer materials for dangerous materials in a proof-of-concept experiment
- Including an "induced lethality" or "kill-switch" device
Safe Lab Work
What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!
Safe Shipment
Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?